Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ...Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.展开更多
为了解决传统ASN.1(abstract syntax notation one)编译码工作中存在的缺陷,根据TD-SCDMA中ASN.1编译码原理,提出利用编译器自动生成工具another tool for language recognition(ANTLR),设计了一个ASN.1描述代码的编译器,实现从ASN.1源...为了解决传统ASN.1(abstract syntax notation one)编译码工作中存在的缺陷,根据TD-SCDMA中ASN.1编译码原理,提出利用编译器自动生成工具another tool for language recognition(ANTLR),设计了一个ASN.1描述代码的编译器,实现从ASN.1源代码到CSharp(C#)语言数据结构的映射,其中包含完整的编译码所需信息,且便于访问。通过调用独立的编译码算法函数,从数据结构中提取相应的参数完成编译码。实际应用表明该编译系统减省了繁复的人工翻译描述代码工作,提高了ASN.1编译码的效率和准确率。展开更多
目的探讨DLC-1(deleted in liver cancer 1,DLC-1)、编码原钙黏蛋白10(protocadherin-10,PCDH10)表达水平和依赖还原型辅酶Ⅰ/Ⅱ:醌氧化还原酶NAD(P)H:quinone oxidoreductase l,NQO1基因多态性与吸烟诱导胃癌行内镜黏膜下剥离术(ESD)...目的探讨DLC-1(deleted in liver cancer 1,DLC-1)、编码原钙黏蛋白10(protocadherin-10,PCDH10)表达水平和依赖还原型辅酶Ⅰ/Ⅱ:醌氧化还原酶NAD(P)H:quinone oxidoreductase l,NQO1基因多态性与吸烟诱导胃癌行内镜黏膜下剥离术(ESD)术后复发的相关性。方法选取于2015年1月至2018年1月进行ESD术的202例胃癌患者作为研究对象,根据患胃癌前是否吸烟分为吸烟组(n=102)和不吸烟组(n=100),比较2组患者DLC-1、PCDH10表达水平以及NQO1基因多态性。根据患者术后复发情况分为复发组和未复发组,并分析上述基因多态性与患者复发之间的相关性。结果吸烟组DLC-1阳性表达比例显著低于不吸烟组患者(P<0.05)。另外染色结果表明:DLC-1在正常胃组织、癌旁组织和癌组织中表达水平分别呈现高表达、中表达、低表达,免疫反应性逐渐减弱;吸烟组患者PCDH10 mRNA表达量显著高于不吸烟组(P<0.05),且2组患者胃癌组织PCDH10 mRNA表达量显著高于癌旁组织(P<0.05);术后发生胃癌复发患者的DCL-1阴性率、胃癌组织PCDH10 mRNA水平以及NQO1基因CT和TT型比例均显著高于未复发组患者(P<0.05);Logistic回归分析结果发现DCL-1阴性率、胃癌组织PCDH10 mRNA水平以及NQO1基因多态性均与胃癌患者ESD术后复发存在密切相关性(P<0.05)。结论吸烟、NQOI基因多态性、DLC-1阴性和PCDH10高表达会增加胃癌发生风险,DLC-1阴性与胃癌ESD术后复发存在显著负相关,吸烟、PCDH10表达水平以及NQO1基因多态性与胃癌ESD术后复发存在显著正相关。展开更多
基金funded by the National Key Research and Development Program of China(Nos.2019YFE0190900,2018YFD0900705).
文摘Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.
文摘为了解决传统ASN.1(abstract syntax notation one)编译码工作中存在的缺陷,根据TD-SCDMA中ASN.1编译码原理,提出利用编译器自动生成工具another tool for language recognition(ANTLR),设计了一个ASN.1描述代码的编译器,实现从ASN.1源代码到CSharp(C#)语言数据结构的映射,其中包含完整的编译码所需信息,且便于访问。通过调用独立的编译码算法函数,从数据结构中提取相应的参数完成编译码。实际应用表明该编译系统减省了繁复的人工翻译描述代码工作,提高了ASN.1编译码的效率和准确率。
