The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulce...The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.展开更多
Tilapia is a freshwater fish group with a sustainable prospect but suffers off-notes appearing during cooking processes.To promote pleasant odorants by thermal cooking processes,tilapia fillets were cooked in differen...Tilapia is a freshwater fish group with a sustainable prospect but suffers off-notes appearing during cooking processes.To promote pleasant odorants by thermal cooking processes,tilapia fillets were cooked in different ways(roasting,microwave-heating,boiling and steaming).Their aroma profiles were analysed with special focus on off-notes and umami-enhancing odorants by principal component analysis,and correlated with the heating time,colour,moisture and water activity by partial least squares regression analysis.Results showed that the“green”and“earthy”off-notes were highly correlated with the boiling process(excess of water,short heating time),while most of the umami-enhancing odorants had a high association with the roasting process(low water content,long heating time,better Maillard reaction).This study indicated that roasting is the most adapted cooking process promoting Maillard-derived aromas,umami-enhancing aromas and meanwhile,reducing off-notes.This research helps in understanding the off-note generation in tilapia and promoting desirable umami-enhancing odorants.展开更多
Fish feed is one of the main constraints on the growth of aquaculture in Kenya. The lack of high-quality and competitively priced feeds means that the fish farmers are unable to expand their production. To ensure that...Fish feed is one of the main constraints on the growth of aquaculture in Kenya. The lack of high-quality and competitively priced feeds means that the fish farmers are unable to expand their production. To ensure that aquaculture remains viable and sustainable, it is necessary to optimize production through the utilization of locally available feeding materials. Insect-based feeds are alternative least-cost feeding resource in fish production. Using alternative protein sources such as black soldier fly larvae (BSF) and daphnia meal in place of fishmeal (Omena and Dagaa) is an ingenious strategy for sustainable fish aquaculture. This study evaluated the effect of four treatments: BSF, daphnia, Omena from Lake Victoria and Dagaa from Lake Tanganyika on the fish pond environmental parameters and fish fries’ performance (body weight and length). The experiment was carried out in a greenhouse with 30 fingerlings in each experimental unit. The feeding trials took 23 days. Results indicated that the four treatments have a varied effect on the BW and L of fish fries. A high growth rate was observed in Daphia, followed by BSF, Omena and Dagaa in that order. On the other hand, the treatments have varied effects on pond parameters with BSF promoting too much growth of algae. BSF have higher amounts of ammonia, nitrates and phosphorus, which stimulate the high growth of algae.展开更多
Pampanga is the top tilapia-producing province in Philippines. One of its municipalities, Minalin, is considered as one of its major centers of production. This study aims to determine the impacts of climate change ha...Pampanga is the top tilapia-producing province in Philippines. One of its municipalities, Minalin, is considered as one of its major centers of production. This study aims to determine the impacts of climate change hazards on the economic performance of tilapia-intensive pond culture in Minalin, Pampanga considering the threats to the industry, livelihoods, and food security. Results revealed that the economic performance of tilapia pond operations using semi-intensive large-scale culture was affected by climate change, as reflected in the reduction of yield among tilapia pond operators. This makes intensive tilapia pond farming in Minalin still a viable venture but will now require more than a year to pay for investment (variable and fixed) costs if under climate change risks. The study recommends strengthening capacity building related to climate-smart tilapia farming technologies for pond operators and to implement appropriate technologies to address climate change risks.展开更多
This study was carried out in 2022 at the Itato agriculture community estate of fish hatchery in eastern Senegal with the aim of finding an alternative method to 17-α-methyl testosterone (MT) by using Garcinia kola ...This study was carried out in 2022 at the Itato agriculture community estate of fish hatchery in eastern Senegal with the aim of finding an alternative method to 17-α-methyl testosterone (MT) by using Garcinia kola “Bitter kola” powder to obtain Oreochromis niloticus male sex populations. 1800 fry of average size between 0.01 and 0.02 g were distributed in 12 aboveground tanks of 1 m3 each at a rate of 150 individuals/tank, following 6 duplicate treatments. The negative (B1 and B2) and positive (B3 and B4) control diets, to which between 0 and 60 mg/kg of 17alpha-methyl testosterone had been added respectively, were mixed with industrial feed containing 40% protein;the tank diets (B5 and B6), (B7 and B8), (B9 and B10) and (B11 and B12) contained 10 g, 20 g, 30 g and 40 g of Garcinia kola powder respectively for 1 kg of industrial feed con-taining 40% protein. These different treatments resulted in high proportions of males in the order of 97.03%;98.09% for the hormone and proportions of 94.03%;93.26% for 20 g of Garcinia kola extracts. In terms of weight growth, the best average weights for the hormone and Garcinia kola were 0.896 g and 0.980 g respectively. With regard to fish survival, the following results were recorded: 95.53% and 94.56% respectively for additions of 20 g and 30 g of Garcinia kola extracts to the feed supplied to the fish. On the other hand, a significant difference in the mortality rate of 35% and 5% respectively for the conventional hormone and 20 g of Garcinia kola extracts was observed. To conclude the study, it is recommended that further studies be carried out to determine the quality of the flesh, their life stages and the profile of the amino acids present in their proteins.展开更多
基金supported by the National Key R&D Pro-grams of China(No.2018YFD0901103)the Hainan Provincial Natural Science Foundation of China(No.2019 RC093).
文摘The aim of this experiment was to investigate the ameliorative effect and molecular mechanism of tilapia head glycolipid(TH-GL)on indomethacin(IDM)-induced gastric ulcer in male Sprague Dawley(SD)rats.The gastric ulcer model was established by oral administration of 30mgkg^(-1) IDM after 7 days of TH-GL or omeprazole(OME)administration in rats.Then the macroscopic gastric injury symptoms,gastric mucosa protective factor cyclooxygenase 1(COX-1),cyclooxygenase 2(COX-2),prostaglandin E_(2)(PGE_(2)),the levels of oxidative stress,and inflammatory cytokine expression levels in the rats were analyzed.The experimental results showed that multiple ulcers appeared on the gastric surface of the rats in the model group.Compared to the model group,TH-GL significantly alleviated gastric ulcers and reduced the gastric damage index in rats.In addition,TH-GL significantly promoted the expression of constitutive enzyme COX-1 while inhibited the expression of inducible enzyme COX-2,and make PGE2 maintain at normal levels.TH-GL also inhibited oxidative stress and inflammatory responses,increased superoxide dismutase(SOD)activity and glutathione(GSH)content,decreased the level of malondialdehyde(MDA)and the content of pro-inflammatory factor.In conclusion,these results suggested that TH-GL could maintain the expression levels of COX-1 and PGE2 while inhibit the expression of COX-2 in the gastric of rat and then prevent IDM-induced gastric ulcer,which may be related to the regulation of oxidative stress and inflammatory response.Therefore,TH-GL might be a new option for the prevention of gastric diseases induced by IDM.
基金supported in part by the China Scholarship Council Fund
文摘Tilapia is a freshwater fish group with a sustainable prospect but suffers off-notes appearing during cooking processes.To promote pleasant odorants by thermal cooking processes,tilapia fillets were cooked in different ways(roasting,microwave-heating,boiling and steaming).Their aroma profiles were analysed with special focus on off-notes and umami-enhancing odorants by principal component analysis,and correlated with the heating time,colour,moisture and water activity by partial least squares regression analysis.Results showed that the“green”and“earthy”off-notes were highly correlated with the boiling process(excess of water,short heating time),while most of the umami-enhancing odorants had a high association with the roasting process(low water content,long heating time,better Maillard reaction).This study indicated that roasting is the most adapted cooking process promoting Maillard-derived aromas,umami-enhancing aromas and meanwhile,reducing off-notes.This research helps in understanding the off-note generation in tilapia and promoting desirable umami-enhancing odorants.
文摘Fish feed is one of the main constraints on the growth of aquaculture in Kenya. The lack of high-quality and competitively priced feeds means that the fish farmers are unable to expand their production. To ensure that aquaculture remains viable and sustainable, it is necessary to optimize production through the utilization of locally available feeding materials. Insect-based feeds are alternative least-cost feeding resource in fish production. Using alternative protein sources such as black soldier fly larvae (BSF) and daphnia meal in place of fishmeal (Omena and Dagaa) is an ingenious strategy for sustainable fish aquaculture. This study evaluated the effect of four treatments: BSF, daphnia, Omena from Lake Victoria and Dagaa from Lake Tanganyika on the fish pond environmental parameters and fish fries’ performance (body weight and length). The experiment was carried out in a greenhouse with 30 fingerlings in each experimental unit. The feeding trials took 23 days. Results indicated that the four treatments have a varied effect on the BW and L of fish fries. A high growth rate was observed in Daphia, followed by BSF, Omena and Dagaa in that order. On the other hand, the treatments have varied effects on pond parameters with BSF promoting too much growth of algae. BSF have higher amounts of ammonia, nitrates and phosphorus, which stimulate the high growth of algae.
文摘Pampanga is the top tilapia-producing province in Philippines. One of its municipalities, Minalin, is considered as one of its major centers of production. This study aims to determine the impacts of climate change hazards on the economic performance of tilapia-intensive pond culture in Minalin, Pampanga considering the threats to the industry, livelihoods, and food security. Results revealed that the economic performance of tilapia pond operations using semi-intensive large-scale culture was affected by climate change, as reflected in the reduction of yield among tilapia pond operators. This makes intensive tilapia pond farming in Minalin still a viable venture but will now require more than a year to pay for investment (variable and fixed) costs if under climate change risks. The study recommends strengthening capacity building related to climate-smart tilapia farming technologies for pond operators and to implement appropriate technologies to address climate change risks.
文摘This study was carried out in 2022 at the Itato agriculture community estate of fish hatchery in eastern Senegal with the aim of finding an alternative method to 17-α-methyl testosterone (MT) by using Garcinia kola “Bitter kola” powder to obtain Oreochromis niloticus male sex populations. 1800 fry of average size between 0.01 and 0.02 g were distributed in 12 aboveground tanks of 1 m3 each at a rate of 150 individuals/tank, following 6 duplicate treatments. The negative (B1 and B2) and positive (B3 and B4) control diets, to which between 0 and 60 mg/kg of 17alpha-methyl testosterone had been added respectively, were mixed with industrial feed containing 40% protein;the tank diets (B5 and B6), (B7 and B8), (B9 and B10) and (B11 and B12) contained 10 g, 20 g, 30 g and 40 g of Garcinia kola powder respectively for 1 kg of industrial feed con-taining 40% protein. These different treatments resulted in high proportions of males in the order of 97.03%;98.09% for the hormone and proportions of 94.03%;93.26% for 20 g of Garcinia kola extracts. In terms of weight growth, the best average weights for the hormone and Garcinia kola were 0.896 g and 0.980 g respectively. With regard to fish survival, the following results were recorded: 95.53% and 94.56% respectively for additions of 20 g and 30 g of Garcinia kola extracts to the feed supplied to the fish. On the other hand, a significant difference in the mortality rate of 35% and 5% respectively for the conventional hormone and 20 g of Garcinia kola extracts was observed. To conclude the study, it is recommended that further studies be carried out to determine the quality of the flesh, their life stages and the profile of the amino acids present in their proteins.