以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列...以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列比对分析,设计了检测小麦印度腥黑穗病菌及黑麦草腥黑穗病菌的TaqM an MGB实时荧光PCR引物和探针,优化了反应条件,筛选出特异性探针,分别建立了小麦印度腥黑穗病菌和黑麦草腥黑穗病菌实时荧光单重PCR和实时荧光双重PCR检测方法,其中实时荧光双重PCR检测方法实现了在同一PCR管中仅用5μL的反应体系,进行1次PCR反应就能特异性检测出小麦印度腥黑穗病菌或黑麦草腥黑穗病菌。本研究所建立的检测方法特异性强、结果可靠、检测速度快、成本明显降低,在实际应用中具有推广价值。展开更多
The role of lignifications and enzymes involved in the phenylpropanoid (PP) biosynthesis i.e. phenylalanine ammonia lyase (PAL), Peroxidase (POD), Polyphenol oxidase (PPO) in providing resistance to Karnal Bunt (KB) d...The role of lignifications and enzymes involved in the phenylpropanoid (PP) biosynthesis i.e. phenylalanine ammonia lyase (PAL), Peroxidase (POD), Polyphenol oxidase (PPO) in providing resistance to Karnal Bunt (KB) during different developmental stages of resistant (HD-29) and susceptible genotype (WH-542) and its recombinant inbred lines (RILs) of wheat were investigated. The enzymes of PP pathway were expressed constitutively in both the susceptible and resistant genotype. However, the activity was higher in all the developmental stages of resistant genotype and its RILs, indicating that this genotype has a significant higher basal level of these enzymes as compared to the susceptible line and could be used as marker(s) to define KB resistance. The activity of PAL and POD was significantly higher in WSv stage (Z = 16) while the specific activity of PPO was higher in WS3 (Z = 77) stage as compared to the other physiological stages in both the genotypes. In resistant genotype the lignin content increased two-fold and three-fold at WS2 and WS3 stage, respectively, while in susceptible genotype no significant increase in lignin content was observed. The pathway might be associated with the enhancement of structural defense barrier due to lignifications of cell wall as evident from the enhanced synthesis of lignin in all the stages of resistant genotype. Our results clearly indicate the possible role of enzymes of PP metabolism provides genotype and stage dependant structural barrier resistance in wheat against KB.展开更多
文摘以小麦印度腥黑穗病菌9个菌株和黑麦草腥黑穗病菌5个菌株及其近似种或相关种:稻粒黑粉菌、狼尾草腥黑粉菌、狗尾草腥黑粉菌、苏玛特腥黑粉菌、狐尾草腥黑粉菌、小麦网腥黑穗病菌和小麦矮化腥黑穗病菌共9种22个菌株为研究对象,通过序列比对分析,设计了检测小麦印度腥黑穗病菌及黑麦草腥黑穗病菌的TaqM an MGB实时荧光PCR引物和探针,优化了反应条件,筛选出特异性探针,分别建立了小麦印度腥黑穗病菌和黑麦草腥黑穗病菌实时荧光单重PCR和实时荧光双重PCR检测方法,其中实时荧光双重PCR检测方法实现了在同一PCR管中仅用5μL的反应体系,进行1次PCR反应就能特异性检测出小麦印度腥黑穗病菌或黑麦草腥黑穗病菌。本研究所建立的检测方法特异性强、结果可靠、检测速度快、成本明显降低,在实际应用中具有推广价值。
文摘利用光学显微镜和扫描电镜对小麦印度腥黑粉菌及其近似种的形态学特征进行了系统研究。T. indica和T. horrida不同菌株冬孢子大小变化范围均较大。在所研究的种中,T. indica与T. walkeri最近似,前者冬孢子大小平均值比后者略大,分别为:38.35?5.92祄和32.86?1.53祄。T. indica与其它具有疣状或刺状突起的腥黑粉菌:T. horrida, T. barclayana, T. setariae, T. opaca, T. sumatii和T. savilei等区别明显,T. indica冬孢子大小平均值明显大于这些腥黑粉菌,前者大于30祄,后者则小于30祄, T. indica孢壁纹饰与这些腥黑粉菌也有一定区别。应用光学显微镜和扫描电镜,能将T. indica与除T. walkeri之外的其它近似种区别开,但在区别T. indica与T. walkeri方面则有一定的局限性。
文摘The role of lignifications and enzymes involved in the phenylpropanoid (PP) biosynthesis i.e. phenylalanine ammonia lyase (PAL), Peroxidase (POD), Polyphenol oxidase (PPO) in providing resistance to Karnal Bunt (KB) during different developmental stages of resistant (HD-29) and susceptible genotype (WH-542) and its recombinant inbred lines (RILs) of wheat were investigated. The enzymes of PP pathway were expressed constitutively in both the susceptible and resistant genotype. However, the activity was higher in all the developmental stages of resistant genotype and its RILs, indicating that this genotype has a significant higher basal level of these enzymes as compared to the susceptible line and could be used as marker(s) to define KB resistance. The activity of PAL and POD was significantly higher in WSv stage (Z = 16) while the specific activity of PPO was higher in WS3 (Z = 77) stage as compared to the other physiological stages in both the genotypes. In resistant genotype the lignin content increased two-fold and three-fold at WS2 and WS3 stage, respectively, while in susceptible genotype no significant increase in lignin content was observed. The pathway might be associated with the enhancement of structural defense barrier due to lignifications of cell wall as evident from the enhanced synthesis of lignin in all the stages of resistant genotype. Our results clearly indicate the possible role of enzymes of PP metabolism provides genotype and stage dependant structural barrier resistance in wheat against KB.
