Selected suitable seed cell, scaffold and growth factor could maximize the repair effect using tissue engineering method in spinal cord injury

Spinal cord injury usually leads to permanent disability, which could cause a huge financial problem to the patient. Up to now there is no effective method to treat this disease. The key of the treatment is to enable the damage zone axonal regeneration and luckily it could go through the damage zone; last a connection can be established with the target neurons. This study attempts to combine stem cell, material science and genetic modification technology together, by preparing two genes modified adipose-derived stem cells and inducing them into neuron direction; then by compositing them on the silk fibroin/chitosan scaffold and implanting them into the spinal cord injury model, seed cells can have features of neuron cells. At the same time, it could stably express the brain-derived neurotrophic factor and neurotrophin-3, both of which could produce synergistic effects, which have a positive effect on the recovery of spinal cord. The spinal cord scaffold bridges the broken end of the spinal cord and isolates with the surrounding environment, which could avoid a scar effect on the nerve regeneration and provide three-dimensional space for the seed cell growth, and at last we hope to provide a new treatment for spinal cord injury with the tissue engineering technique.

Biomaterials and tissue engineering in traumatic brain injury:novel perspectives on promoting neural regeneration

Traumatic brain injury is a serious medical condition that can be attributed to falls, motor vehicle accidents, sports injuries and acts of violence, causing a series of neural injuries and neuropsychiatric symptoms. However, limited accessibility to the injury sites, complicated histological and anatomical structure, intricate cellular and extracellular milieu, lack of regenerative capacity in the native cells, vast variety of damage routes, and the insufficient time available for treatment have restricted the widespread application of several therapeutic methods in cases of central nervous system injury. Tissue engineering and regenerative medicine have emerged as innovative approaches in the field of nerve regeneration. By combining biomaterials, stem cells, and growth factors, these approaches have provided a platform for developing effective treatments for neural injuries, which can offer the potential to restore neural function, improve patient outcomes, and reduce the need for drugs and invasive surgical procedures. Biomaterials have shown advantages in promoting neural development, inhibiting glial scar formation, and providing a suitable biomimetic neural microenvironment, which makes their application promising in the field of neural regeneration. For instance, bioactive scaffolds loaded with stem cells can provide a biocompatible and biodegradable milieu. Furthermore, stem cells-derived exosomes combine the advantages of stem cells, avoid the risk of immune rejection, cooperate with biomaterials to enhance their biological functions, and exert stable functions, thereby inducing angiogenesis and neural regeneration in patients with traumatic brain injury and promoting the recovery of brain function. Unfortunately, biomaterials have shown positive effects in the laboratory, but when similar materials are used in clinical studies of human central nervous system regeneration, their efficacy is unsatisfactory. Here, we review the characteristics and properties of various bioactive materials, followed by the introduction of applications based on biochemistry and cell molecules, and discuss the emerging role of biomaterials in promoting neural regeneration. Further, we summarize the adaptive biomaterials infused with exosomes produced from stem cells and stem cells themselves for the treatment of traumatic brain injury. Finally, we present the main limitations of biomaterials for the treatment of traumatic brain injury and offer insights into their future potential.

Research Progress of Osteochondral Composite Scaffolds in Tissue Engineering Cartilage Repair

Repair and regeneration of articular cartilage has always been a major challenge in the medical field due to its peculiar structure(e.g.sparsely distributed chondrocytes,no blood supply).Cartilage tissue engineering is one promising strategy for cartilage repair,however,one critical issue for cartilage tissue engineering is the integration between tissue-engineered and native cartilage.In recent years,osteochondral tissue engineering has attracted growing interest for overcoming this problem.Herein,we review the development of osteochondral tissue engineering.Firstly,currently used seed cells in osteochondral tissue engineering will be described.Secondly,several types of scaffolds and their(dis)advantage for osteochondral tissue engineering will be introduced.Thirdly,the growth factors currently used in osteochondral tissue engineering will be presented and discussed.

A novel porcine acellular dermal matrix scaffold used in periodontal regeneration

Regeneration of periodontal tissue is the most promising method for restoring periodontal structures.To find a suitable bioactive three- dimensional scaffold promoting cell proliferation and differentiation is critical in periodontal tissue engineering.The objective of this study was to evaluate the biocompatibility of a novel porcine acellular dermal matrix as periodontal tissue scaffolds both in vitro and in vivo.The scaffolds in this study were purified porcine acellular dermal matrix(PADM) and hydroxyapatite-treated PADM(HA-PADM). The biodegradation patterns of the scaffolds were evaluated in vitro.The biocompatibility of the scaffolds in vivo was assessed by implanting them into the sacrospinal muscle of 20 New Zealand white rabbits.The hPDL cells were cultured with PADM or HA-PADM scaffolds for 3,7,14,21 and 28 days.Cell viability assay,scanning electron microscopy(SEM),hematoxylin and eosin(H&E) staining, immunohistochemistry and confocal microscopy were used to evaluate the biocompatibility of the scaffolds.In vitro,both PADM and HA-PADM scaffolds displayed appropriate biodegradation pattern,and also,demonstrated favorable tissue compatibility without tissue necrosis,fibrosis and other abnormal response.The absorbance readings of the WST-1 assay were increased with the time course, suggesting the cell proliferation in the scaffolds.The hPDL cells attaching,spreading and morphology on the surface of the scaffold were visualized by SEM,H&E staining,immnuohjstochemistry and confocal microscopy,demonstrated that hPDL cells were able to grow into the HA-PADM scaffolds and the amount of cells were growing up in the course of time.This study proved that HA-PADM scaffold had good biocompatibility in animals in vivo and appropriate biodegrading characteristics in vitro.The hPDL cells were able to proliferate and migrate into the scaffold.These observations may suggest that HA-PADM scaffold is a potential cell carrier for periodontal tissue regeneration.

The Use of Zein and Shuanghuangbu for Periodontal Tissue Engineering

Aim Tissue engineering is a promising area with a broad range of applications in the fields of regenerative medicine and human health. The emergence of periodontal tissue engineering for clinical treatment of periodontal disease has opened a new therapeutic avenue. The choice of scaffold is crucial. This study was conducted to prepare zein scaffold and explore the suitability of zein and Shuanghuangbu for periodontal tissue engineering.Methodology A zein scaffold was made using the solvent casting/particulate leaching method with sodium chloride （NaC1） particles as the porogen. The physical properties of the zein scaffold were evaluated by observing its shape and determining its pore structure and porosity. Cytotoxicity testing of the scaffold was carried out via in vitro cell culture experiments, including a liquid extraction experi- ment and the direct contact assay. Also, the Chinese medicine Shuanghuangbu, as a growth factor, was diluted by scaffold extract into different concentrations. This Shuanghuangbu-scaffold extract was then added to periodontal ligament cells （PDLCs） in order to determineits effect on cell proliferation. Results The zein scaffold displayed a sponge-like structure with a high porosity and sufficient thickness. The porosity and pore size of the zein scaffold can be controlled by changing the porogen particles dosage and size. The porosity was up to 64.1%-78.0%. The pores were well-distributed, interconnected, and porous. The toxicity of the zein scaffold was graded as 0-1. Furthermore, PDLCs displayed full stretching and vigorous growth under scanning electronic microscope （SEM）. Shuanghuangbu-scaffold extract could reinforce proliferation activity of PDLCs compared to the control group, especially at 100 μg.mL^-1 （P〈0.01）. Conclusion A zein scaffold with high porosity, open pore wall structure, and good biocompatibility is conducive to the growth of PDLCs. Zein could be used as scaffold to repair periodontal tissue defects. Also, Shuanghuangbuscaffold extract can enhance the proliferation activity of PDLCs. Altogether, these findings provide the basis for in vivo testing on animals.

组织工程技术修复颞下颌关节:问题与挑战

背景:颞下颌关节疾病的传统疗法受限于疾病严重程度和个体差异。相比之下,组织工程作为一种新兴治疗方法,可以根据患者具体情况定制个性化治疗方案,减少手术过程中的不确定性,提高治疗效果。目的:综述组织工程修复颞下颌关节的最新研究成果和进展。方法:以“颞下颌关节,组织工程,种子细胞,支架,生长因子,动物模型”为中文检索词,以“temporomandibular joint,tissue engineering,seed cell,scaffold,growth factor,animal model”为英文检索词,分别在PubMed数据库和中国知网进行文献检索,检索时限为各数据库建库时间至2024年3月。通过分析和阅读文献进行筛选,按照排除筛选标准纳入文献,最终纳入57篇文献进行综述。结果与结论:(1)随着生物学、材料学与工程学等技术的发展,颞下颌关节组织工程己取得较大进展,例如种子细胞的筛选、新型支架的开发、生长因子作用机制的探索、多种动物模型的构建等,目前大多数研究尚处于体外实验阶段,动物实验等体内研究尚未大规模开展,组织工程修复颞下颌关节的临床应用还需更多证据支持。(2)尽管颞下颌关节组织工程研究仍存在许多问题和挑战等待解决,依然展现出广阔的临床应用前景,有望在将来成为颞下颌关节疾病出色高效的治疗方式。

组织工程治疗股骨头坏死软骨下分离的适用技术

背景:股骨头坏死出现新月征是病情进程的“分水岭”,修复和稳定骨-软骨界面对阻止病情继续进展和预防股骨头塌陷尤为重要。利用组织工程学同步修复、整合骨-软骨界面具有潜在优势。目的:综述探讨解决股骨头坏死软骨下分离的潜在适宜技术。方法:检索1970年1月至2023年4月PubMed、Web of Science及中国知网、万方数据库中发表的相关文献,英文检索词:“Femoral head necrosis,Avascular necrosis of femoral head,Osteonecrosis of femoral head”等,中文检索词:“股骨头坏死,软骨下骨,软骨,软骨与软骨下骨整合”等,最终纳入114篇文献进行综述分析。结果与结论:①结构缺陷、缺血缺氧环境、炎症因素和应力集中可能造成股骨头坏死软骨下分离现象,软骨下骨分离会造成塌陷进展,并且可能与保髋手术失败相关,利用组织工程支架实现支架与骨-软骨界面的整合是治疗股骨头坏死软骨下分离的潜在方法之一。②目前的文献研究表明,多相、梯度支架和复合材料在促进骨、软骨细胞黏附与增殖,骨软骨基质的沉积方面均有提升,有助于支架与骨-软骨界面的整合,对治疗股骨头坏死软骨下分离有参考价值。③通过对支架表面进行修饰可以提高与界面整合的效率,但有各自不同的优缺点,提供不同环境的支架能够诱导同种间充质干细胞差异分化,有助于不同界面之间的整合。④未来有望应用于股骨头坏死软骨下分离的支架应为复合材料,具有梯度化和差异化的仿生结构,通过表面修饰和干细胞加载促进骨-软骨界面与支架的整合以实现治疗目的,但仍需进一步研究验证,而支架的降解速率与修复进度同步和不同界面之间的稳定性是未来需要解决的主要问题。

食管组织工程:研究、应用与展望

食管作为人体消化系统的重要组成部分,其损伤与疾病严重影响着患者的健康与生活质量。近年来,组织工程技术在食管修复和重建中取得显著进展。本文旨在探讨食管组织工程在食管损伤修复和重建中的应用研究,以应对传统食管替代术带来的高并发症风险。本文综述了支架材料、种子细胞、生长因子等关键要素在组织工程食管构建中的一些最新研究进展,并总结了组织工程技术在内镜黏膜下剥离术后食管修复中的应用情况。最后,本文讨论了食管组织工程面临的挑战与未来研究方向,以期为食管组织工程的发展及转化提供参考。

石墨烯族纳米材料调控骨再生微环境的研究进展

石墨烯族纳米材料(graphene‐family nanomaterials,GFNs)以其卓越的机械性能、生物相容性和促进干细胞成骨分化的能力而在骨组织工程领域备受关注。GFNs在调控骨再生微环境中发挥了多方面的作用。首先,GFNs本身微观形态能够激活黏着斑激酶/细胞外调节蛋白激酶(focal adhesion kinase/extracellular regulated protein kinase,FAK/ERK)信号通路,促进成骨相关基因的表达。其次,GFNs适应骨组织的机械强度,有助于维持骨整合,并通过调整细胞外基质的硬度,借助黏着斑(focal adhesions,FAs)将基质的力学信号传递到胞内,创造良好的理化微环境;此外,它们还能调节骨缺损部位的免疫微环境,引导巨噬细胞向M2型极化,并影响相关细胞因子的分泌。GFNs还可作为生物活性分子的缓释载体,兼具促进血管形成和抗菌能力,从而加速骨缺损的修复过程。同时,GFNs通过多种形式调控骨再生微环境,包括支架材料、水凝胶、生物薄膜和植入物涂层等。尽管GFNs在骨组织工程领域引起广泛关注,但其在骨组织再生方面的应用仍处于基础实验阶段。为了推动GFNs进入临床应用阶段,需要提供更充分的生物相容性证据,明确诱导干细胞成骨分化的机制,并开发更高效的材料应用形式。

基于脂肪干细胞的软骨组织工程的研究新进展

软骨缺损的修复一直是临床上的难题。近年来,以脂肪干细胞(Adipose stem cells,ASCs)为种子细胞的软骨组织工程取得了重要进展,为软骨的修复提供了新方法,但尚不成熟。本文通过查阅国内外文献,就ASCs、相关的细胞因子以及各种新型的仿生支架等在软骨组织工程中的最新研究进展及存在的问题进行综述。文献复习结果表明,ASCs具有来源丰富、免疫原性低等优势,尤其向软骨细胞分化的能力,使其有希望成为软骨组织工程理想的种子细胞。研究发现ASCs分泌的相关细胞因子,尤其是ASCs分泌的多种细胞外囊泡、外泌体(其内含有的多种非编码RNA)具有促进ASCs软骨分化、抑制软骨细胞凋亡等作用。另外,近年来软骨组织工程支架的研发也得到很大进展,如3D打印支架、氧化石墨烯的纳米材料支架、多种天然和人工合成材料构建的复合仿生支架等。目前存在的问题是:ASCs的成软骨诱导效率尚需进一步提高,以及开发出更适合体内微环境的3D仿生支架等,这将是软骨组织工程今后研究的重点。

应用细胞-支架构建方式的组织工程方法促进牙周组织再生的实验研究

目的:观察评价细胞 -支架构建方式的组织工程方法对牙周组织再生修复的影响和意义,探讨自体牙周膜细胞(PDLCs)和纳米羟基磷灰石材料 -纳米羟晶 -胶原仿生骨材料 (nHAC)分别用作牙周组织工程种子细胞和支架材料的可行性。方法:改良组织块法体外培养动物自体PDLCs,传代扩增后接种到nHAC三维支架上,再植入动物人工牙周组织缺损,表面覆盖聚四氟乙烯 (e-PTFE)膜,以单纯翻瓣组和单纯GTR组作为对照。术后 8周进行组织学观察和测量,分析评价其牙周组织的再生情况。结果:自体PDLCs-nHAC-膜复合植入组较单纯翻瓣组和单纯GTR组有更多的新生牙槽骨、新生牙周膜和新生牙骨质生成,且未见上皮长入。结论:应用细胞-支架构建方式的牙周组织工程方法能更有效地促进牙周组织再生和重建,而自体PDLCs可作为牙周组织工程的种子细胞,nHAC可作为牙周组织工程的支架材料。

狗牙周膜细胞三维立体培养的体外实验研究

目的 :探讨异体脱矿松质骨基质 (CBM )和纳米羟基磷灰石材料 (nHAC)两种支架材料应用于牙周组织工程的可行性 ,为建立牙周组织工程的动物模型及下一步进行牙周组织工程的动物实验奠定实验基础。方法 :组织块法培养狗牙周膜细胞 ,传代扩增后 ,接种于CBM和nHAC 2种三维支架上 ,体外继续培养 3d ,进行细胞计数和扫描电镜观察。结果 :狗牙周膜细胞在 2种支架材料上均能形成良好贴附并增殖 ,扫描电镜可见 2种支架材料均具有良好的多孔网状结构 ,细胞在支架材料上生长旺盛 ,伸展充分。结论

一种新型纳米羟基磷灰石材料应用于牙周组织工程的可行性研究

目的:对一种新型纳米羟基磷灰石材料 -纳米羟晶 -胶原仿生骨材料 (nHAC)进行细胞相容性和细胞毒性的研究,以初步探讨其应用于牙周组织工程的可行性。方法:将体外培养的人牙周膜细胞(PDLCs)接种于nHAC三维支架上复合培养,采用细胞计数和扫描电镜观察PDLCs在nHAC支架上的附着、生长情况,并通过MTT测试法和碱性磷酸酶(ALP)活性检测法研究nHAC浸提液对PDLCs增殖和功能表达的影响。结果:人PDLCs在nHAC支架上形成良好贴附并增殖,扫描电镜可见nHAC具有良好的多孔网状结构,细胞在nHAC上生长旺盛,伸展充分,而PDLCs在不同浓度的材料浸提液中的生长、增殖及ALP活性与空白对照组相比无显著性差异。结论:nHAC具有良好的三维空间结构和细胞相容性,且无细胞毒性,有望成为牙周组织工程的支架材料。

人牙周膜细胞体外三维立体培养模型的建立

目的 :建立人牙周膜细胞 (PDLC)体外三维立体培养模型 ,初步探讨两种支架材料应用于牙周组织工程的可行性 ,为进一步研究牙周组织生理病理及牙周组织工程奠定实验基础。方法 :组织块法培养人PDLC ,传代扩增后 ,接种于珊瑚和珊瑚转化羟基磷灰石 (CHA)两种三维支架上 ,体外继续培养 3d ,进行细胞计数和扫描电镜观察。结果 :细胞在两种支架材料上均能形成良好贴附并增殖 ,扫描电镜可见两种支架材料均具有良好的多孔网状结构 ,细胞在支架材料上生长旺盛 ,伸展充分。结论 :可通过将人PDLC接种到珊瑚或CHA上建立体外三维立体培养模型 ,珊瑚和CHA均有望成为牙周组织工程的支架材料。

去细胞猪主动脉瓣叶的获取和内皮细胞的种植

目的 探讨猪主动脉瓣叶去细胞后作为组织工程心脏瓣膜支架的可行性。 方法 经胰酶 - EDTA、表面活性剂和核酸酶处理 ,去除猪主动脉瓣叶的细胞成分 ,测定瓣叶去细胞前、后的生物力学特性 ,并在其表面种植新生牛主动脉内皮细胞 (BAECs) ;分别行大鼠皮下包埋实验。 结果 猪主动脉瓣叶中的细胞成分能完全去除 ,获得完整无细胞的纤维网状支架 ,断裂强度和断裂伸长率无明显变化 ;种植的 BAECs在去细胞瓣叶表面可形成一层连续的细胞层 ,其分泌前列环素 (PGI2 )的能力同直接种植在 2 4孔板中的比较 ,差异无统计学意义 (P>0 .0 5 )。 结论 猪主动脉瓣去细胞后获得的纤维支架可以用来构建组织工程瓣膜 ,适宜于血管内皮细胞的生长。

磷酸三钙-透明质酸-Ⅰ型胶原-骨髓基质细胞复合修复骨缺损的实验研究

目的观察由磷酸三钙人工骨(β-tricalciumphosphate,β-TCP)、透明质酸(hyaluronicacid,HA)、型胶原(type collagen,COL-)复合物作为诱导后的骨髓基质细胞(marrowstromalcells,MSCs)载体修复兔桡骨骨缺损的能力及作为自体骨移植替代物的可能性。方法获取新西兰大白兔MSCs,体外诱导培养后与β-TCP、HA、COL-结合形成复合物。将6月龄新西兰大白兔30只,手术制备双侧桡骨2cm骨缺损,8周后随机分为A、B及C组,A组(n=27侧),植入β-TCP-HA-COL--MSCs;B组(n=27侧),植入自体骨;C组(n=6侧),缺损空置作为空白对照。用扫描电镜观察β-TCP-HA-COL-结构。于4、8和12周各时间点分别处死动物6、9和15只;4、8周时A、B组各6侧,12周时A、B组各15侧;C组8周时6侧。进行大体观察、X线摄片、HE染色及无机质含量测定,对A、B组12周时标本进行成骨面积及生物力学测试,比较各组骨缺损的修复效果。结果MSCs在体外生长稳定,增殖能力强,可被诱导为成骨细胞。β-TCP-HA-COL-复合物呈多孔结构。各组各时间点大体观察、X线片、组织学及生物力学测试结果显示,随着时间的延长A、B组骨缺损可被修复,空白对照组不能修复。12周时成骨面积、生物力学测试,A、B组差异无统计学意义(P>0.05)。A组中的无机质含量在4、8和12周分别为75%、57%和42%。

聚乙烯醇编织构建组织工程前交叉韧带的初步研究

[目的]探讨聚乙烯醇体外编织构建组织工程前交叉韧带支架材料的可行性。[方法]用聚乙烯醇纺丝纤维编织构建韧带支架材料,在电子拉力机上测试该支架材料的力学性质;用组织块和胶原酶消化培养法在体外分离培养人前交叉韧带细胞并对细胞的生长形态和分泌胶原蛋白的特征进行检测,细胞经体外扩增后种植于编织构建的聚乙烯醇韧带支架材料上观察。[结果]韧带支架材料的柔韧性强,拉力测试的负荷—拉伸曲线与韧带的拉伸曲线相似,其最大负荷、极限应力和弹性模量分别为52.61 N、14.96 MPa和202.08 MPa;体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征,能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质;支架材料无细胞毒性,人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。[结论]支架材料具有一定的力学性能和优良的生物相容性,有望成为一种组织工程前交叉韧带支架材料。

骨髓间充质细胞复合胶原-壳聚糖材料联合骨搬移修复胫骨缺损:随机对照实验方案

背景:骨髓间充质干细胞发挥成骨作用需要支架材料的辅助,一方面支架材料不仅可将细胞运载至骨缺损区域,另一方面还可作为新骨生长的框架结构。胶原-壳聚糖复合材料是骨组织工程较为理想的支架材料之一,同时其具有骨诱导性,比常规支架材料更优越的成骨能力。骨搬移技术在临床上在修复长段骨缺损方面已得到广泛应用,但也存在成骨慢、外固定时间长、骨不连等缺憾。如何进一步加快骨形成速度,减少并发症发生,已成当前亟待解决的问题。实验假设:骨髓间充质干细胞复合胶原-壳聚糖支架移植能提高胫骨缺损骨搬移修复效果。方法/设计:随机对照动物实验。分为体外和体内实验两部分。体外实验中取月龄一两个月的新西兰大白兔股骨骨髓,提取骨髓间充质干细胞,培养至第3代,将细胞悬液滴于胶原-壳聚糖支架材料,构建骨髓间充质干细胞复合胶原-壳聚糖支架。体内实验选用24只三四月龄新西兰大白兔,被随机分配接受如下干预:骨搬移、支架植入、骨搬移联合支架植入。研究的主要观察指标为植入材料与骨缺损界面的生长情况、X射线检测的缺损区骨修复情况、苏木精-伊红染色及扫描电镜观察缺损区成骨情况、免疫组织化学染色检测成骨区Ⅰ型胶原蛋白的表达情况、扫描电子显微镜观察移植材料与宿主骨的界面键合情况、超微结构及新骨的生成。讨论:实验结果将有助于确定对骨缺损进行骨搬移治疗过程中,应用骨髓间充质干细胞复合胶原-壳聚糖支架移植促进骨缺损再生修复效果的可行性。实验方案获基金支持情况:获辽宁省科学技术计划项目资助(2012225019)。

新型生物可降解材料与骨髓间充质干细胞生物相容性研究

目的 探讨人骨髓间充质干细胞 (MSCs)与第 3代聚羟基烷酸酯 (PHA)类聚酯 :3-羟基丁酸、3-羟基己酸无规嵌段共聚物 [p(3HB- co- 3HH) ]的生物相容性 ,以及材料植入体内的组织相容性。 方法 将培养的人 MSCs分别接种于细胞载体 p(3HB- co- 3HH)上 ,通过相差显微镜和电镜 ,以及 HE、Von Kossa染色和 型胶原表达等分析 ,了解细胞载体复合物体外立体培养 2周、或裸鼠体内植入后经过培养的 MSCs在支架材料上生长、扩增和分泌基质等与材料的相容性。 结果 p(3HB- co- 3HH)具有良好的亲水性及细胞亲和力 ,材料表面不需特殊处理 (如卵磷脂、多聚赖氨酸包埋等 ) ,可以作为种子细胞的载体 ,接种的 MSCs能够在材料中均匀分布 ,贴壁生长良好 ,维持骨髓干细胞表型。在培养体系中加入成骨诱导分化试剂后 ,贴壁生长的 MSCs可向成骨细胞分化并分泌基质 ,表达 型胶原。 结论 p(3HB- co-3HH)具有良好的生物相容性和细胞亲和性 ,是较好的可降解的高分子生物材料。

组织工程细胞支架及其细胞亲和性改进研究进展

综述近年来组织工程中有关细胞在材料上粘附的机理研究并从生物学观点与材料学观点来分析影响细胞亲和性的因素 ,介绍了目前研究中的改性生物医学材料细胞亲和性的研究方法 。