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Reaction of antibodies to Campylobacter jejuni and cytolethal distending toxin B with tissues and food antigens 被引量:3
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作者 Aristo Vojdani Elroy Vojdani 《World Journal of Gastroenterology》 SCIE CAS 2019年第9期1050-1066,共17页
BACKGROUND The bacteria Campylobacter jejuni(C. jejuni) is commonly associated with GuillaneBarré syndrome(GBS) and irritable bowel syndrome(IBS), but studies have also linked it with Miller Fisher syndrome, reac... BACKGROUND The bacteria Campylobacter jejuni(C. jejuni) is commonly associated with GuillaneBarré syndrome(GBS) and irritable bowel syndrome(IBS), but studies have also linked it with Miller Fisher syndrome, reactive arthritis and other disorders, some of which are autoimmune. It is possible that C. jejuni and its toxins may be crossreactive with some human tissues and food antigens, potentially leading to autoimmune responses.AIM To measure the immune reactivity of C. jejuni and C. jejuni cytolethal distending toxin(Cdt) antibodies with tissue and food antigens to examine their role in autoimmunities.METHODS Using enzyme-linked immunosorbent assay(ELISA) methodology, specific antibodies made against C. jejuni and C. jejuni Cdt were applied to a variety of microwell plates coated with 45 tissues and 180 food antigens. The resulting immunoreactivities were compared to reactions with control wells coated with human serum albumin(HSA) which were used as negative controls and with wells coated with C. jejuni lysate or C. jejuni Cdt which served as positive controls.RESULTS At 3 SD above the mean of control wells coated with HSA or 0.41 OD, the mouse monoclonal antibody made against C. jejuni showed moderate to high reactions with zonulin, somatotropin, acetylcholine receptor, β-amyloid and presenilin.This immune reaction was low with an additional 25 tissue antigens including asialoganglioside, and the same antibody did not react at all with another 15 tissue antigens. Examining the reaction between C. jejuni antibody and 180 food antigens, we found insignificant reactions with 163 foods but low to high immune reactions with 17 food antigens. Similarly, we examined the reaction of C. jejuni Cdt with the same tissues and food antigens. The strongest reactions were observed with zonulin, intrinsic factor and somatotropin. The reaction was moderate with 9 different tissue antigens including thyroid peroxidase, and reaction was low with another 10 different antigens, including neuronal antigens.The reaction of C. jejuni Cdt antibody with an additional 23 tissue antigens was insignificant. Regarding the reaction of C. jejuni Cdt antibody with different food antigens, 160 out of 180 foods showed insignificant reactions, while 20 foods showed reactions ranging from low to high.CONCLUSION Our findings indicate that C. jejuni and its Cdt may play a role in inflammation and autoimmunities beyond the gut. 展开更多
关键词 CAMPYLOBACTER JEJUNI Cytolethal distending TOXIN tissue antigenS Food antigenS Autoimmune REACTIVITIES CROSS-REACTIVITY
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Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues 被引量:7
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作者 Bin Yan An-Chun Cheng +5 位作者 Ming-Shu Wang Shu-Xuan Deng Zhen-Hua Zhang Nian-Chun Yin Ping Cao Sheng-Yan Cao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第5期776-781,共6页
AIM: To detect Salmonella enteritidis (S. enteritidis) in paraffin slices and antigen location in infected duck tissues. METHODS: The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific... AIM: To detect Salmonella enteritidis (S. enteritidis) in paraffin slices and antigen location in infected duck tissues. METHODS: The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis. RESULTS: The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.CONCLUSION: IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium. 展开更多
关键词 Indirect immuno-fluorescent antibody staining Salrnonella enteritidis Paraffin tissues DETECTION Localization of antigens
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Profile of Autoantibodies and Clinical Symptoms in Guinean Patients with Connective Tissue Diseases
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作者 Mariama Sadjo Diallo Doufin Traore +13 位作者 Adame Diouf Djiba Kaba Issiaga Diallo Doudou Georges Massar Niang Alimou Camara Oumar Mouctar Diallo Boh Fanta Diane Abdoulaye Barry Ouo-Ouo Yaramon Kolie Moustapha Mbow Aly Badra Kamissoko Djibril Sylla Babacar Mbengue Mohamed Cisse 《Open Journal of Immunology》 2024年第1期1-10,共10页
Connective tissue diseases (CTDs) are Autoimmune diseases (AIDs) characterized by the appearance of autoantibodies, which are diagnostic markers. Investigations of these autoantibodies play a major role in the managem... Connective tissue diseases (CTDs) are Autoimmune diseases (AIDs) characterized by the appearance of autoantibodies, which are diagnostic markers. Investigations of these autoantibodies play a major role in the management of several autoimmune diseases. The objective of this study was to describe the profile of anti-ENA antibodies according to the clinical symptoms of mixed CTDs in Conakry teaching Hospital. We performed a cross-sectional study during six months. A total of 20 patients was recruited and we measured antibodies using the ELISA technique. The mean age of our patients was 36.5 years, with a predominance of females. Cutaneous and rheumatological signs were the main clinical manifestations. SLP was the most frequent CTDs;the threshold of ENA antibodies positivity was higher in scleroderma with and SLP. Anti-ENA identification reveals the frequency of anti-SSA (83.33%), anti-U1RNP (66.66%) and anti-histone (50%) antibodies. Antinuclear antibodies (ANA) react with various components of the cell nucleus. Their detection is of major interest in the diagnosis of CTDs. Our results highlight the importance of determining the specificity of these antibodies to guide differential diagnosis. 展开更多
关键词 AUTOANTIBODIES Extractible Nuclear antigen (ENA) Connective tissue Diseases ELISA
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Pathological changes and viral antigen distribution in the lymphatic tissue of Patients with epidemic hemorrhagic fever
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作者 刘彦仿 杨守京 +2 位作者 黄高昇 晏培松 徐志凯 《Journal of Medical Colleges of PLA(China)》 CAS 1994年第1期69-71,共3页
Pathological changes and viral antigen distribution in the lymphatic tissue of Patients with epidemic hemorr... Pathological changes and viral antigen distribution in the lymphatic tissue of Patients with epidemic hemorrhagic feverLiuYan... 展开更多
关键词 EPIDEMIC HEMORRHAGIC FEVER lymphoid tissue antigens immunohistochemistry PATHOLOGICAL CHANGES patients
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Cross-Reaction between Gliadin and Different Food and Tissue Antigens
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作者 Aristo Vojdani Igal Tarash 《Food and Nutrition Sciences》 2013年第1期20-32,共13页
A subgroup of coeliac disease patients continues to experience symptoms even on a gluten-free diet (GFD). We attempted to determine whether these symptoms could be due to either cross-contamination with gluten-contain... A subgroup of coeliac disease patients continues to experience symptoms even on a gluten-free diet (GFD). We attempted to determine whether these symptoms could be due to either cross-contamination with gluten-containing foods or cross-reactivity between α-gliadin and non-gluten foods consumed on a GFD. We measured the reactivity of affinity-purified polyclonal and monoclonal α-gliadin 33-mer peptide antibodies against gliadin and additional food antigens commonly consumed by patients on a GFD using ELISA and dot-blot. We also examined the immune reactivity of these antibodies with various tissue antigens. We observed significant immune reactivity when these antibodies were applied to cow’s milk, milk chocolate, milk butyrophilin, whey protein, casein, yeast, oats, corn, millet, instant coffee and rice. To investigate whether there was cross-reactivity between α-gliadin antibody and different tissue antigens, we measured the degree to which this antibody bound to these antigens. The most significant binding occurred with asialoganglioside, hepatocyte, glutamic acid decarboxylase 65, adrenal 21-hydroxylase, and various neural antigens. The specificity of anti-α-gliadin binding to different food and tissue antigens was demonstrated by absorption and inhibition studies. We also observed significant cross-reactivity between α-gliadin 33-mer and various food antigens, but some of these reactions were associated with the contamination of non-gluten foods with traces of gluten. The consumption of cross-reactive foods as well as gluten-contaminated foods may be responsible for the continuing symptoms presented by a subgroup of patients with coeliac disease. The lack of response of some CD patients may also be due to antibody cross-reactivity with non-gliadin foods. These should then be treated as gluten-like peptides and should also be excluded from the diet when the GFD seems to fail. 展开更多
关键词 CROSS-REACTION GLIADIN FOOD antigenS tissue antigenS CELIAC Disease GLUTEN-FREE Diet
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Cryptococcal antigen testing of lung tissue homogenate improves pulmonary cryptococcosis diagnosis:Two case reports
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作者 Wei-Yi Wang Yu-Lu Zheng Li-Bin Jiang 《World Journal of Clinical Cases》 SCIE 2022年第12期3893-3898,共6页
BACKGROUND Pulmonary cryptococcosis(PC)is an opportunistic infectious disease of the respiratory system.Lung tissue biopsies,culture of respiratory samples(e.g.,sputum,lung tissue,pleural fluid,and bronchoalveolar lav... BACKGROUND Pulmonary cryptococcosis(PC)is an opportunistic infectious disease of the respiratory system.Lung tissue biopsies,culture of respiratory samples(e.g.,sputum,lung tissue,pleural fluid,and bronchoalveolar lavage fluid),and cryptococcal antigen(CrAg)testing are helpful for a definitive diagnosis.However,these tests are sometimes falsely negative.PC is often misdiagnosed or underdiagnosed owing to the absence of obvert symptoms,poor imaging specificity,and false-negative laboratory tests.CASE SUMMARY We report two female patients who underwent computed tomography-guided percutaneous needle pulmonary biopsy of a lung nodule for a confirmed diagnosis.In both patients,the CrAg test on the lung biopsy tissue homogenate was positive,while the serum CrAg test was negative.Combined with the lung tissue pathology,we made the diagnosis of PC.Antifungal therapy was effective in both patients.CONCLUSION Given the findings of our cases and the literature review,lung tissue homogenate CrAg testing can be helpful in improving the diagnosis of PC. 展开更多
关键词 Lung tissue homogenate Cryptococcal antigen test Pulmonary cryptococcosis Lung biopsy DIAGNOSIS Case report
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DIAGNOSTIC VALUE OF SERUM PANCREATIC TISSUE CARCINOMA ANTIGEN IN PANCREATIC CARCINOMA
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作者 孟济明 许国铭 +3 位作者 沈茜 张洪富 吴月琴 姚光弼 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第3期71-73,共3页
Serum pancreatic tissue carcinoma antigen (PCA) was measured by ELISA in 396 patients with pancreatic carcinoma (PC) and other diseases diagnosed by clinical examination, histopathology and operation. Serum PCA more t... Serum pancreatic tissue carcinoma antigen (PCA) was measured by ELISA in 396 patients with pancreatic carcinoma (PC) and other diseases diagnosed by clinical examination, histopathology and operation. Serum PCA more than 300 U/ml was considered as diagnostic value for PC and 30-299 U/ml as probable index for PC. The sensitivity of PCA for PC was 78.2% (43/55), and the specifity 94.6%. The positive rate of PCA in other diseases were 0-13.3%. Combining the determination of PCA with B-mode ultrasonic scanning (BU). CT and/or ERCP (endos-copic retrograde cholangiopancreatography) may increase the diagnostic rate of PC to 89-91%, and with the "cocktail" of CA19-9, CEA (carcino-embryonic antigen) and RNase-C, PC be diagnosed in 88.9%. Therefore, it is suggest that PCA si a promising tumor market of PC with high sensitivity and relative specificity. 展开更多
关键词 DIAGNOSTIC VALUE OF SERUM PANCREATIC tissue CARCINOMA antigen IN PANCREATIC CARCINOMA PC ERCP
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Tissu plasminogen-activator antigen (t-pAAg) thrombotic marker in patients with myoc ardial infarction
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《中国输血杂志》 CAS CSCD 2001年第S1期367-,共1页
关键词 tissu plasminogen-activator antigen t-pAAg
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Study on Distribution of HFRS Virus in the Tissuses and Cells of Human Body
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作者 杨为松 姚志强 +6 位作者 陈思毅 王晶翼 唐永明 张文彬 白宪光 白雪帆 朱平 《Journal of Medical Colleges of PLA(China)》 CAS 1989年第2期105-107,共3页
Immunochemical rnethods,viral tissue culture and dlectron microscopic observationtechiniques were used to study the distribution of HFRS virus in the tissues and cells of patients withHFRS and sorne of their abotive f... Immunochemical rnethods,viral tissue culture and dlectron microscopic observationtechiniques were used to study the distribution of HFRS virus in the tissues and cells of patients withHFRS and sorne of their abotive faus.The results showed that HFRS virus could infect many hu-man organs and cells and pass through plaoenta and blood-brain barrier,and was characteristic ofpantropical infection,the duration of the viremia of the patients being mostly near a week.Thestudy is valuable for elucidation pathogenesis of the disease. 展开更多
关键词 HEMORRHAGIC FEVER with renal syndrome(JFRS) IMMUNOHISTOCHEMICAL VIRAL antigen VIRAL tissue culture electron microscope
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False positive detection of serum cryptococcal antigens due to insufficient sample dilution:A case series
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作者 Wen-Yu Chen Cheng Zhong +1 位作者 Jian-Ying Zhou Hua Zhou 《World Journal of Clinical Cases》 SCIE 2023年第8期1837-1846,共10页
At present,with the development of technology,the detection of cryptococcal antigen(CRAG)plays an increasingly important role in the diagnosis of cryptococcosis.However,the three major CRAG detection technologies,late... At present,with the development of technology,the detection of cryptococcal antigen(CRAG)plays an increasingly important role in the diagnosis of cryptococcosis.However,the three major CRAG detection technologies,latex agglutination test(LA),lateral flow assay(LFA)and Enzyme-linked Immunosorbent Assay,have certain limitations.Although these techniques do not often lead to false-positive results,once this result occurs in a particular group of patients(such as human immunodeficiency virus patients),it might lead to severe consequences. 展开更多
关键词 CRYPTOCOCCOSIS Capsular antigen detection False positive tissue Case report
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Transcription Activity of Ectogenic Human Carcinoembryonic Antigen Promoter in Lung Adenocarcinoma Cells A549
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作者 熊维宁 方慧娟 +5 位作者 徐永健 熊盛道 曹勇 宋青凤 曾大雄 张惠兰 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第5期517-519,共3页
The transcription activity of ectogenic human carcinoembryonic antigen (CEA) promoter in lung adenocarcinoma cells A549 was investigated for the further gene-targeting therapy. The reporter gene green fluorescent pr... The transcription activity of ectogenic human carcinoembryonic antigen (CEA) promoter in lung adenocarcinoma cells A549 was investigated for the further gene-targeting therapy. The reporter gene green fluorescent protein (GFP) driven by CEA promoter and human cytomegalovirus (CMV) promoter were relatively constructed and named plasmid pCEA-EGFP and pCMV-GFP respectively. The intensity of fluorescence was detected by fluorescence microscope and flow cytometry analysis after the pCEA-GFP and pSNAV-GFP plasmids were transfected into A549 cells through liposome respectively. The results showed (4,08±0.63) % of the A549 cells transfected with pCEA-AFP plasmid expressed, significantly lower than that of the A549 cells transfected with pCMV-GFP [(43.27±3.54) %]. It was suggested that ectogenic human CEA promoter in lung adenocarcinoma cells A549 was weakly expressed. The distinct specificity of CEA promoter in CEA high expression cells was regarded as a tool in selective gene therapy, but the transcription activity of ectogenic human CEA promoter was needed to increase in the future. 展开更多
关键词 tissue-specific promoter carcinoembryonic antigen lung carcinoma
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Leukocyte function-associated antigen-1 deficiency impairs responses to polymicrobial sepsis
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作者 Jia-Ren Liu Xiaohui Han +1 位作者 Sulpicio G Soriano Koichi Yuki 《World Journal of Clinical Cases》 SCIE 2015年第9期793-806,共14页
AIM: To determine the role of leukocyte functionassociated antigen-1(LFA-1) in polymicrobial sepsis model in mice.METHODS: Cecal ligation and puncture model was used to study polymicrobial sepsis in wild type and LFA-... AIM: To determine the role of leukocyte functionassociated antigen-1(LFA-1) in polymicrobial sepsis model in mice.METHODS: Cecal ligation and puncture model was used to study polymicrobial sepsis in wild type and LFA-1 knockout(KO)(= CD11 a KO) mice. Their survivals were examined. Neutrophil recruitment to the abdominal cavity, bacterial tissue load and bacterial killing by neutrophils, tissue cytokine profiles, and serum cytokines were examined. Apoptosis of tissues was assessed using cleaved-caspase 3 and TUNNEL staining. The recruitment of neutrophils to various tissues was assessed using myeloperoxidase staining or measuring myeloperoxidase activity. RESULTS: LFA-1 deficiency significantly decreased survival(P = 0.0024) with the reduction of neutrophil recruitment to the abdominal cavity and higher bacterial load in blood. It was also associated with increased apoptosis in spleen and more organ injuries probed by interleukin-6 m RNA level. However, the deficiency of LFA-1 did not prevent neutrophil recruitment to lung, liver, spleen or kidney, which suggested the existence of LFA-1 independent recruitment mechanism in these organs. CONCLUSION: LFA-1 deficiency did not attenuate neutrophil recruitment to various organs to adequately mitigate secondary tissue injury in sepsis. It was associated with decreased neutrophil recruitment to the abdominal cavity, higher bacterial load, leading to increased mortality in an abdominal, polymicrobial sepsis. 展开更多
关键词 LEUKOCYTE function-associated antigen-1 tissue injury NEUTROPHIL RECRUITMENT POLYMICROBIAL sepsis Apoptosis
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EBNA2免疫组化在EB病毒阳性淋巴组织疾病中的表达意义
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作者 张燕林 贺文 +3 位作者 谢建兰 郑晓丹 郑媛媛 周小鸽 《诊断病理学杂志》 2024年第4期300-303,共4页
目的观察EBNA2免疫组化在不同EB病毒阳性淋巴组织疾病中的表达差异、分析其在病理诊断与鉴别诊断中的价值。方法收集344例EB病毒阳性的淋巴组织疾病,总结EBNA2免疫组化在病变中的表达特点。结果不同的EBV阳性淋巴组织病变,EBNA2的阳性... 目的观察EBNA2免疫组化在不同EB病毒阳性淋巴组织疾病中的表达差异、分析其在病理诊断与鉴别诊断中的价值。方法收集344例EB病毒阳性的淋巴组织疾病,总结EBNA2免疫组化在病变中的表达特点。结果不同的EBV阳性淋巴组织病变,EBNA2的阳性表达不同:IM中阳性39例(39/46),EBV阳性的弥漫大B细胞淋巴瘤3例(3/30),淋巴瘤样肉芽肿1例(1/5),纤维素相关弥漫大B细胞淋巴瘤2例(2/2),浆母细胞淋巴瘤1例(1/14),免疫相关淋巴组织增殖性疾病32例(32/58)(包括移植后淋巴组织增殖性疾病21例,HIV相关Burkitt淋巴瘤1例,HIV相关弥漫大B细胞淋巴瘤2例,原发免疫缺陷疾病相关淋巴组织疾病6例,医源性免疫相关淋巴组织增殖性疾病2例)。其余均显示EBNA2阴性。②不同病变中的EBNA2阳性细胞表达明显不同:传染性单核细胞增多症中的EBNA2阳性细胞相对较少,并且多呈散在分布,几乎很少见到簇状聚集的情况。EBNA2阳性细胞多表现为≤10个/HPF,少见11~50个/HPF,仅有个别病例显示大于50个/HPF。免疫相关淋巴组织增殖性疾病中,出现大量EBNA2阳性细胞,阳性细胞较弥漫分布,大部分为强阳性,并且阳性细胞多显示大于50个/HPF。结论EBNA2阳性在疾病的诊断及鉴别诊断提供了新的依据,具有一定的价值;当病变表达EBNA2时,提示患者可能存在免疫缺陷或处于免疫失调状态,对临床诊断及治疗具有一定的指导意义。 展开更多
关键词 EB病毒 淋巴组织疾病 传染性单核细胞增多症 EBV核抗原2
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皮肤石蜡组织Ⅳ型胶原免疫荧光抗原修复方法的评估
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作者 张彬 李治 +1 位作者 金晶 吴滢 《实用皮肤病学杂志》 2024年第2期89-92,共4页
目的 寻找皮肤石蜡包埋组织Ⅳ型胶原免疫荧光(IF-PE)最佳抗原修复方法。方法以2021年1月至2022年12月在上海交通大学医学院附属儿童医院确诊的X-连锁Alport综合征患儿和父(母)亲共14例皮肤组织,冰冻组织免疫荧光(IF-FT)为自身对照,比较... 目的 寻找皮肤石蜡包埋组织Ⅳ型胶原免疫荧光(IF-PE)最佳抗原修复方法。方法以2021年1月至2022年12月在上海交通大学医学院附属儿童医院确诊的X-连锁Alport综合征患儿和父(母)亲共14例皮肤组织,冰冻组织免疫荧光(IF-FT)为自身对照,比较不同抗原修复法IF-PE染色部位和强度。结果 与Ⅳ型胶原IF-FT自身对照比较,采用蛋白酶K修复,仅部分患儿基膜正常分布(6/14),而角质层均异常表达;微波、高温水浴和高压蒸汽3种修复方法,基膜均无正常分布,角质层异常分布(分别为2/14,11/14,12/14);微波联合酶修复法仅2例基膜正常分布,未见角质层异常表达;高压蒸汽联合酶和高温水浴联合酶2种修复法基膜全部正常分布,角质层异常表达(分别为14/14和2/14),表达强度两者接近。结论高温水浴联合蛋白酶K可作为皮肤石蜡包埋组织Ⅳ型胶原免疫荧光理想的抗原修复法。 展开更多
关键词 皮肤组织 石蜡包埋免疫荧光 抗原修复 Ⅳ型胶原
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乳腺癌患者HER2、CA153表达与声触诊组织成像定量技术参数的相关性分析
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作者 丁颖 李家菊 王国亮 《中国医学装备》 2024年第3期78-81,107,共5页
目的:分析乳腺癌患者人表皮生长因子受体2(HER2)、糖类抗原153(CA153)表达与声触诊组织成像定量(VTIQ)技术参数的相关性。方法:选取2018年5月至2020年5月合肥市第三人民医院收治的80例女性乳腺癌患者,其中世界卫生组织(WHO)分期Ⅰ期14... 目的:分析乳腺癌患者人表皮生长因子受体2(HER2)、糖类抗原153(CA153)表达与声触诊组织成像定量(VTIQ)技术参数的相关性。方法:选取2018年5月至2020年5月合肥市第三人民医院收治的80例女性乳腺癌患者,其中世界卫生组织(WHO)分期Ⅰ期14例、Ⅱ期22例、Ⅲ期31例、Ⅳ期13例;另选取同时间段收治的53例女性乳腺良性疾病患者为对照。所有患者先行常规超声检查,随后进入超声VTIQ成像模式获得剪切波速度(SWV)均值参数;采用免疫组织化学法检测乳腺组织中HER2表达,采用罗氏E411电化学发光免疫分析仪检测血清CA153水平;采用Pearson法分析乳腺癌患者血清CA153水平与SWV均值的相关性。结果:与良性患者比较,乳腺癌患者VTIQ技术参数SWV均值、血清CA153水平及HRR2阳性表达率均显著升高,差异有统计学意义(F=39.107,78.353,P<0.05);与乳腺癌Ⅰ+Ⅱ期患者比较,乳腺癌Ⅲ、Ⅳ期患者VTIQ技术参数SWV均值、血清CA153水平、HRR2阳性表达率均显著升高,差异有统计学意义(t=2.685、3.556、8.326、10.455,P<0.05);与乳腺癌Ⅲ期比较,乳腺癌Ⅳ期患者VTIQ技术参数SWV均值、血清CA153水平、HRR2阳性表达率均显著升高,差异有统计学意义(t=4.632、8.659,P<0.05)。与HER2阴性表达乳腺癌患者SWV均值比较,HER2阳性表达乳腺癌患者SWV均值升高,差异有统计学意义(χ^(2)=59.751,P<0.05)。乳腺癌患者血清CA153水平与SWV均值呈正相关(r=0.501,P<0.05)。结论:乳腺癌患者VTIQ参数SWV均值与乳腺癌患者生物标志物HER2、CA153表达水平密切相关。 展开更多
关键词 乳腺癌 声触诊组织成像定量技术 人表皮生长因子受体2(HER2) 糖类抗原153(CA153)
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养血散寒通脉方治疗血虚寒凝型子宫内膜异位症的临床研究 被引量:2
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作者 黄艳辉 吴绘春 《广州中医药大学学报》 CAS 2024年第3期618-625,共8页
【目的】观察养血散寒通脉方(由当归四逆加吴茱萸生姜汤加减而成)治疗血虚寒凝型子宫内膜异位症(EMS)的临床疗效。【方法】将120例血虚寒凝型EMS患者随机分为研究组和对照组,每组各60例。研究组给予养血散寒通脉方治疗,对照组给予少腹... 【目的】观察养血散寒通脉方(由当归四逆加吴茱萸生姜汤加减而成)治疗血虚寒凝型子宫内膜异位症(EMS)的临床疗效。【方法】将120例血虚寒凝型EMS患者随机分为研究组和对照组,每组各60例。研究组给予养血散寒通脉方治疗,对照组给予少腹逐瘀颗粒治疗,疗程为3个月,并于疗程结束后随访1年。观察2组患者治疗前后各种疼痛评分[包括经期腹痛视觉模拟量表(VAS)评分和痛经、非经期盆腔痛、性交痛、盆腔压痛、骶韧带结节触痛分级评分]、卵巢子宫内膜异位囊肿大小及血清糖类抗原125(CA125)、血管内皮生长因子(VEGF)、可溶性细胞间黏附分子1(SICAM-1)、基质金属蛋白酶9(MMP-9)、组织金属蛋白酶抑制因子2(TIMP-2)水平的变化情况,并观察2组患者的临床疗效、安全性、复发情况及妊娠情况。【结果】(1)研究过程中,研究组脱落3例,对照组脱落5例,最终共112例患者纳入统计分析,其中研究组57例,对照组55例。(2)治疗3个月后,研究组的总有效率为92.98%(53/57),对照组为85.45%(47/55),组间比较,研究组的疗效明显优于对照组(P<0.05)。(3)治疗后,2组患者的各种疼痛评分(包括经期腹痛VAS评分和痛经、非经期盆腔痛、性交痛、盆腔压痛、骶韧带结节触痛分级评分)均较治疗前明显下降(P<0.05),且研究组的下降幅度均明显优于对照组(P<0.05)。(4)治疗后,2组患者的卵巢子宫内膜异位囊肿均略有缩小,但组内治疗前后及治疗后组间比较,差异均无统计学意义(P>0.05)。(5)治疗后,2组患者血清CA125、VEGF、SICAM-1、MMP-9水平均较治疗前下降(P<0.05),血清TIMP-2水平均较治疗前升高(P<0.05),且研究组对血清CA125、VEGF、SICAM-1、MMP-9水平的下降幅度及对血清TIMP-2水平的升高幅度均明显优于对照组(P<0.05)。(6)随访1年,研究组的复发率为30.19%(16/53),明显低于对照组的68.09%(32/47),组间比较,差异有统计学意义(P<0.05)。研究组21例有生育要求,其中14例妊娠,妊娠率为66.67%(14/21);对照组20例有生育要求,其中4例妊娠,妊娠率为20.00%(4/20);组间比较,研究组的妊娠率明显高于对照组,差异有统计学意义(P<0.05)。(7)治疗期间,2组患者均无明显不良反应发生,且患者的血、尿、大便常规及心电图、肝肾功能等安全性指标均无异常变化。【结论】养血散寒通脉方治疗血虚寒凝型EMS患者疗效确切,能够显著缓解患者各种疼痛症状,改善妊娠结局,有效调节血清CA125、VEGF、SICAM-1、MMP-9、TIMP-2水平。 展开更多
关键词 子宫内膜异位症 血虚寒凝 养血散寒通脉方 当归四逆加吴茱萸生姜汤 痛经 妊娠结局 糖类抗原125 血管内皮生长因子 可溶性细胞间黏附分子1 基质金属蛋白酶9
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STEAP1在膀胱移形细胞癌中的表达及其潜在诊断价值
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作者 万玛草 白洁 +3 位作者 赵宪龙 常斌 王建平 杨立昌 《国际检验医学杂志》 CAS 2024年第3期272-276,共5页
目的探索前列腺六段扩膜上皮抗原1(STEAP1)在膀胱移行细胞癌中的表达及其潜在诊断价值。方法选取2021年6月至2022年12月于中国人民解放军联勤保障部队第九四O医院行外科手术治疗的52例膀胱移行细胞癌患者作为观察组,另外选取同期年龄、... 目的探索前列腺六段扩膜上皮抗原1(STEAP1)在膀胱移行细胞癌中的表达及其潜在诊断价值。方法选取2021年6月至2022年12月于中国人民解放军联勤保障部队第九四O医院行外科手术治疗的52例膀胱移行细胞癌患者作为观察组,另外选取同期年龄、性别、基础疾病患病率等基础临床资料匹配的膀胱良性肿瘤患者52例作为对照组。通过酶联免疫吸附试验法、实时荧光定量聚合酶链反应测定两组患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平,以及对比不同病理参数患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平。通过Spearman相关性分析、二元Logistic回归分析筛选膀胱移行细胞癌发生及临床分期的危险因素,通过受试者工作特征(ROC)曲线及曲线下面积(AUC)评价各指标对膀胱移行细胞癌的诊断及预测价值。结果观察组膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平均显著高于对照组,差异有统计学意义(P<0.05)。中晚期膀胱移行细胞癌患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平均显著高于早期膀胱移行细胞癌患者,差异有统计学意义(P<0.05)。通过二元Logistic回归分析表明,患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平增高均是膀胱移行细胞癌发生及中晚期膀胱移行细胞癌的独立危险因素(P<0.05)。ROC曲线分析显示,患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA独立预测膀胱移行细胞癌发生的AUC分别为0.841(95%CI:0.760~0.922,P<0.001)、0.936(95%CI:0.893~0.980,P<0.001),均具有较高的预测效能。结论膀胱移行细胞癌患者膀胱肿瘤组织中STEAP1及STEAP1 mRNA相对表达水平与膀胱移行细胞癌发生及中晚期膀胱移行细胞癌呈正相关,提示STEAP1可作为诊断、预测膀胱移行细胞癌发生、发展的潜在标志物。 展开更多
关键词 膀胱移行细胞癌 前列腺六段扩膜上皮抗原1 膀胱肿瘤组织
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基于血清CA50、TSGF、TPA的中晚期乳腺癌放化疗敏感性列线图预测模型的建立与评价 被引量:1
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作者 陈国华 张浩宇 +1 位作者 韩升波 何建鑫 《中国医科大学学报》 CAS 北大核心 2024年第3期240-245,共6页
目的探究血清糖类抗原50(CA50)、肿瘤特异性生长因子(TSGF)、组织多肽抗原(TPA)表达对中晚期乳腺癌放化疗敏感性的预测价值,通过建立列线图模型进行验证与评价。方法选取82例中晚期乳腺癌患者为研究对象,所有患者均接受紫杉醇化疗联合放... 目的探究血清糖类抗原50(CA50)、肿瘤特异性生长因子(TSGF)、组织多肽抗原(TPA)表达对中晚期乳腺癌放化疗敏感性的预测价值,通过建立列线图模型进行验证与评价。方法选取82例中晚期乳腺癌患者为研究对象,所有患者均接受紫杉醇化疗联合放疗,根据实体瘤疗效评价标准分为敏感组(n=57)和不敏感组(n=25)。统计2组患者的一般资料,治疗前后血清CA50、TSGF、TPA表达水平及差值,构建列线图模型,绘制校准曲线、受试者操作特征(ROC)曲线、决策曲线,评估列线图模型的预测效能和临床效用。结果2组比较,肿瘤直径、脉管侵犯、TNM分期、淋巴结转移、分化程度的差异有统计学意义(P<0.05)。治疗后不敏感组血清CA50、TSGF、TPA表达水平高于敏感组,CA50、TSGF、TPA差值低于敏感组(P<0.05)。将上述有统计学差异的因素纳入LASSO回归,选出3个预测变量,分别为CA50、TSGF、TPA差值。logistic回归显示,CA50、TSGF、TPA差值是中晚期乳腺癌放化疗敏感性的影响因素(P<0.05);基于CA50、TSGF、TPA差值构建列线图模型,校准曲线、ROC曲线、决策曲线显示该模型具有良好的预测精准度和临床效用。结论中晚期乳腺癌放化疗不敏感的患者治疗后血清CA50、TSGF、TPA表达水平较高,且三者差值是影响放化疗敏感性的因素。基于血清CA50、TSGF、TPA构建的列线图预测模型具有良好的预测价值和临床效用。 展开更多
关键词 糖类抗原50 肿瘤特异性生长因子 组织多肽抗原 中晚期乳腺癌 放化疗敏感性 列线图模型
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三阴性乳腺癌患者血清组织多肽抗原和糖类抗原的表达及其对复发的预测价值
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作者 张志火 龚玲珍 冯梅 《广东医学》 CAS 2024年第5期636-640,共5页
目的探讨三阴性乳腺癌(triple-negative breast cancer,TNBC)患者血清组织多肽抗原(tissue polypeptide antigen,TPA)与糖类抗原(carbohydrate antigen)CA15-3表达的相关性及其对术后复发事件的预测价值。方法对组织多肽抗原TPA和糖类抗... 目的探讨三阴性乳腺癌(triple-negative breast cancer,TNBC)患者血清组织多肽抗原(tissue polypeptide antigen,TPA)与糖类抗原(carbohydrate antigen)CA15-3表达的相关性及其对术后复发事件的预测价值。方法对组织多肽抗原TPA和糖类抗原CA15-3在三阴性乳腺癌和非三阴乳腺癌患者血清中的表达水平进行组间比较。三阴性乳腺癌患者血清TPA、CA15-3表达的相关性及临床意义进行分析。依据TNBC患者稳定期与术后复发患者检测结果,分析TPA、CA15-3表达对短期复发事件的影响及术后短期复发事件的预测效能。结果三阴性乳腺癌与非三阴乳腺癌患者血清TPA、CA15-3表达水平及TNBC早期、中期患者组间比较,差异有统计学意义(P<0.05)。经Pearson相关性分析,三阴性乳腺癌患者血清TPA、CA15-3表达相关系数在0.01的水平上呈显著正相关。TNBC患者术后复发事件与稳定期组患者血清TPA、CA15-3表达水平比较,差异有统计学意义(P<0.01)。三阴性乳腺癌患者血清TPA、CA15-3表达水平的ROC曲线分析结果显示,预测效能敏感度、特异度有统计学意义。结论组织多肽抗原TPA与糖类抗原CA15-3可能是三阴性乳腺癌患者预后的预测靶基因,作为复发事件的生物学观察指标,具有重要的临床意义。 展开更多
关键词 三阴性乳腺癌 组织多肽抗原 糖类抗原CA15-3 短期复发风险
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类风湿关节炎患者关节滑液及滑膜组织中CCP/AST表达水平及临床意义 被引量:1
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作者 朱玉光 方丽佳 黄素玲 《现代检验医学杂志》 CAS 2024年第1期162-165,174,共5页
目的探究类风湿关节炎(rheumatoid arthritis,RA)患者关节滑液及滑膜组织中环瓜氨酸肽抗原特异性T细胞(cyclic citrullinated peptide antigen-specific T cells,CCP/AST)表达水平及临床意义。方法选取石家庄市中医院2021年1月~12月128... 目的探究类风湿关节炎(rheumatoid arthritis,RA)患者关节滑液及滑膜组织中环瓜氨酸肽抗原特异性T细胞(cyclic citrullinated peptide antigen-specific T cells,CCP/AST)表达水平及临床意义。方法选取石家庄市中医院2021年1月~12月128例RA患者为RA组,并取同期在该院因关节疼痛需做关节镜检查者50例为对照组。其中RA患者轻度组46例,中度组52例和重度组30例;通过蛋白印迹分析各组受试者滑膜组织类风湿因子(rheumatoid factors,RF)和抗瓜氨酸蛋白抗体(anticitrullinated protein antibodies,ACPA)蛋白表达水平;通过流式细胞仪分析受试者关节滑液中CCP/AST频率;双重免疫荧光染色/激光共聚焦扫描观察滑膜组织中CCP/AST染色强度;Pearson相关性分析评估关节滑液、滑膜组织中CCP/AST表达与RF和ACPA的相关性;采用Logistic回归分析RA发生的危险因素。结果对照组、轻度组、中度组和重度组患者滑膜组织中RF(1.01±0.01,1.53±0.03,2.01±0.08,2.66±0.12kDa)和ACPA蛋白(1.03±0.01,1.61±0.03,2.04±0.10,2.59±0.13 kDa)依次升高,差异具有统计学意义(F=14.207,12.446,均P<0.05)。对照组、轻度组、中度组和重度组患者关节滑液中CCP/AST表达依次升高(8.26%±1.68%,22.46%±3.28%,33.58%±4.37%,46.15%±5.44%),差异具有统计学意义(F=25.306,P<0.05)。对照组、轻度组、中度组和重度组患者滑膜组织CCP/AST染色强度依次升高(1.05±0.26,1.35±0.89,2.04±0.56,2.78±0.15分),差异具有统计学意义(F=70.67,P<0.05)。RA患者关节滑液、滑膜组织中CCP/AST表达与RF呈明显正相关(r=0.861,0.934,均P<0.05),与ACPA呈明显正相关(r=0.854,0.913,均P<0.05)。Logistic回归分析结果显示,高血压(OR=3.241,95%CI:1.491~6.752)、糖尿病(OR=2.565,95%CI:1.126~5.813)以及关节滑液(OR=4.450,95%CI:1.652~11.622)、滑膜组织(OR=5.629,95%CI:2.474~12.390)中CCP/AST表达均为RA发生的独立危险因素(P<0.05)。结论RA患者关节滑液及滑膜组织中CCP/AST呈现高表达,且与疾病活动度及关节破坏有关,可用于评估此类患者临床关节活动度及骨破坏程度。 展开更多
关键词 类风湿关节炎 关节滑液 滑膜组织 环瓜氨酸肽抗原特异性T细胞
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