The manipulation of cell behaviors is essential to maintaining cell functions,which plays a critical role in repairing and regenerating damaged tissue.To this end,a rich variety of tissue-engineered scaffolds have bee...The manipulation of cell behaviors is essential to maintaining cell functions,which plays a critical role in repairing and regenerating damaged tissue.To this end,a rich variety of tissue-engineered scaffolds have been designed and fabricated to serve as matrix for supporting cell growth and functionalization.Among others,scaffolds made of electrospun fibers showed great potential in regulating cell behaviors,mainly owing to their capability of replicating the dimension,composition,and function of the natural extracellular matrix.In particular,electrospun fibers provided both topological cues and biofunctions simply by adjusting the electrospinning parameters and/or post-treatment.In this review,we summarized the most recent applications and advances in electrospun nanofibers for manipulating cell behaviors.First,the engineering of the secondary structures of individual fibers and the construction of two-dimensional nanofiber mats and nanofiber-based,three-dimensional scaffolds were introduced.Then,the functionalization strategies,such as endowing the fibers with bioactive,physical,and chemical cues,were explored.Finally,the typical applications of electrospun fibers in controlling cell behaviors(i.e.,cell adhesion and proliferation,infiltration,migration,neurite outgrowth,stem cell differentiation,and cancer cell capture and killing)were demonstrated.Taken together,this review will provide valuable information to the specific design of nanofiber-based scaffolds and extend their use in controlling cell behaviors for the purpose of tissue repair and regeneration.展开更多
基金National Natural Science Foundation of China(32171322,82001970)Natural Science Foundation of Shandong Province(ZR2021QC063,ZR2021YQ17)+3 种基金Young Elite Scientists Sponsorship Program by CAST(No.YESS20200097)State Key Laboratory for Modification of Chemical Fibers and Polymer Materials(KF2215)Qingdao Key Health Discipline Development Fund(2020-2022)Qingdao Clinical Research Center for Oral Diseases(22-3-7-lczx-7-nsh)。
文摘The manipulation of cell behaviors is essential to maintaining cell functions,which plays a critical role in repairing and regenerating damaged tissue.To this end,a rich variety of tissue-engineered scaffolds have been designed and fabricated to serve as matrix for supporting cell growth and functionalization.Among others,scaffolds made of electrospun fibers showed great potential in regulating cell behaviors,mainly owing to their capability of replicating the dimension,composition,and function of the natural extracellular matrix.In particular,electrospun fibers provided both topological cues and biofunctions simply by adjusting the electrospinning parameters and/or post-treatment.In this review,we summarized the most recent applications and advances in electrospun nanofibers for manipulating cell behaviors.First,the engineering of the secondary structures of individual fibers and the construction of two-dimensional nanofiber mats and nanofiber-based,three-dimensional scaffolds were introduced.Then,the functionalization strategies,such as endowing the fibers with bioactive,physical,and chemical cues,were explored.Finally,the typical applications of electrospun fibers in controlling cell behaviors(i.e.,cell adhesion and proliferation,infiltration,migration,neurite outgrowth,stem cell differentiation,and cancer cell capture and killing)were demonstrated.Taken together,this review will provide valuable information to the specific design of nanofiber-based scaffolds and extend their use in controlling cell behaviors for the purpose of tissue repair and regeneration.
