The interaction between titanocene glycine complex and DNA has been investigat-ed by UV-Vis spectra and fluorescence probe method. The results suggest that the complex forms an irreversible adduct with DNA in molar ra...The interaction between titanocene glycine complex and DNA has been investigat-ed by UV-Vis spectra and fluorescence probe method. The results suggest that the complex forms an irreversible adduct with DNA in molar ratio 1:1, and induces the comformation changes of DNA at high ratio of the complex to DNA,leading to the disruption of DNA du-plexical structure.展开更多
Titanocene alanine complex, Cp2Ti(O2CCH(CH3)NH3+Cl-)(2), has been found to have in vitro antitumor activity against Ehrlich ascites tumor cells. Study of the interaction between Cp2Ti(O2CCH(CH3)NH3+Cl-)(2) and DNA by ...Titanocene alanine complex, Cp2Ti(O2CCH(CH3)NH3+Cl-)(2), has been found to have in vitro antitumor activity against Ehrlich ascites tumor cells. Study of the interaction between Cp2Ti(O2CCH(CH3)NH3+Cl-)(2) and DNA by UV-VIS spectra, fluorescence spectra, agarose gel electrophoresis reveals that Cp2Ti(O2CCH(CH3)NH3+Cl-)(2) forms a stable compound with DNA in molar ratio 1:1 and reduces the migration rate of ccc-DNA band and oc-DNA band. However, unlike cisplatin, the complex does not induce nicking of DNA even after a long incubation at a high ratio of the complex to DNA. The results suggest that titanocene-based antitumor agents have different mechanism of action from cisplatin-like antitumor agents.展开更多
文摘The interaction between titanocene glycine complex and DNA has been investigat-ed by UV-Vis spectra and fluorescence probe method. The results suggest that the complex forms an irreversible adduct with DNA in molar ratio 1:1, and induces the comformation changes of DNA at high ratio of the complex to DNA,leading to the disruption of DNA du-plexical structure.
文摘Titanocene alanine complex, Cp2Ti(O2CCH(CH3)NH3+Cl-)(2), has been found to have in vitro antitumor activity against Ehrlich ascites tumor cells. Study of the interaction between Cp2Ti(O2CCH(CH3)NH3+Cl-)(2) and DNA by UV-VIS spectra, fluorescence spectra, agarose gel electrophoresis reveals that Cp2Ti(O2CCH(CH3)NH3+Cl-)(2) forms a stable compound with DNA in molar ratio 1:1 and reduces the migration rate of ccc-DNA band and oc-DNA band. However, unlike cisplatin, the complex does not induce nicking of DNA even after a long incubation at a high ratio of the complex to DNA. The results suggest that titanocene-based antitumor agents have different mechanism of action from cisplatin-like antitumor agents.
