表面增强激光解吸电离飞行时间质谱(Surface-enhanced laser desorption/ionization-time of flight,SELDI-TOF-MS)技术是目前蛋白质组学研究较为有效的手段之一,已被广泛的应用于肿瘤、传染性疾病、心血管病和神经系统等疾病的研究.然...表面增强激光解吸电离飞行时间质谱(Surface-enhanced laser desorption/ionization-time of flight,SELDI-TOF-MS)技术是目前蛋白质组学研究较为有效的手段之一,已被广泛的应用于肿瘤、传染性疾病、心血管病和神经系统等疾病的研究.然而SELDI-TOF-MS技术本身存在着一些问题,如分析结果有效性差和实验重复验证率低等,使其不能完全的应用于常规的临床诊断,现有许多研究致力于解决这些问题,如规范化实验室操作、预处理原始标本和数据、提高统计学方法等极大的改善和优化了这项技术.展开更多
To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni- cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry ...To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni- cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry （UPLC/Q-TOF- MS） and discuss their fragmentation regularity, the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data, tandem characteristic fragment ions and standards. In the end, 12 alkaloids were identi- fied from Ranunculus japonicus for the first time, and their fragmentation regularity was discussed. Thus, a rapid and effec- tive analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established.展开更多
煤矿井下人员定位系统种类千变万化,但主要是在硬件和软件上的差异,因此,选择合适的硬件结构和软件架构会有效地避免井下巷道复杂,环境恶劣,干扰严重等因素.本文设计的基于NXP最新推出的集成片上TOF引擎的JN5168无线微控制器的井下人员...煤矿井下人员定位系统种类千变万化,但主要是在硬件和软件上的差异,因此,选择合适的硬件结构和软件架构会有效地避免井下巷道复杂,环境恶劣,干扰严重等因素.本文设计的基于NXP最新推出的集成片上TOF引擎的JN5168无线微控制器的井下人员定位前端控制系统,支持IEEE802.15.4无线通信协议,只需要设计芯片正常工作必要的外围电路,再通过改进TOF(Time of Flight)测距软件定位算法,便可构成一个精确定位系统.该系统满足成本低、开发时间短、定位误差低、功耗低、实时性快等优点,对煤矿井下复杂环境的人员定位系统的研制具有一定的理论和应用价值.展开更多
物体表面积是物体的基本物理参数,在大学物理实验中有着广泛的应用。然而复杂物体的表面积测量是非常有难度的工作。为实现复杂不规则物体表面积的快速测量,采用脉冲激光TOF(Time Of Flight)技术采集待测物体的三维点云信息,然后对点云...物体表面积是物体的基本物理参数,在大学物理实验中有着广泛的应用。然而复杂物体的表面积测量是非常有难度的工作。为实现复杂不规则物体表面积的快速测量,采用脉冲激光TOF(Time Of Flight)技术采集待测物体的三维点云信息,然后对点云数据进行处理来实现三维模型的重建,最终实现模型的优化以及表面积数据的快速测量。实验结果表明系统能在较短的时间内实现模型重建并快速有效地实现表面积测量。这样的测量方法与传统的测量方法相比具有极大的创新性,系统成本低、不仅操作方便而且简单直观。其新颖性更是大大激发了大学生物理实验的学习兴趣。展开更多
介绍一种基于3D成像的车载客流计数系统,通过飞行时间(Time of Flight,TOF)方式成像,实现对人员、物体、行李的识别。该系统可在每一个车门处对上下车人数进行准确统计,并将统计全车结果实时上传到服务器提供给其他系统使用,实现人员数...介绍一种基于3D成像的车载客流计数系统,通过飞行时间(Time of Flight,TOF)方式成像,实现对人员、物体、行李的识别。该系统可在每一个车门处对上下车人数进行准确统计,并将统计全车结果实时上传到服务器提供给其他系统使用,实现人员数量的准确检测。实验表明,该系统检测准确率高达95%以上,满足业主的需求。展开更多
Cerium （III） tetraphenylporphyrin nitrate Ce（TPP）NO3 was synthesized by using meso- tetraphenylporphyrin （TPP） and Ce（NO3）.6H20 in mixture solution of CHC13 and C2HsOH （V：V=1：1）. The complex was characteri...Cerium （III） tetraphenylporphyrin nitrate Ce（TPP）NO3 was synthesized by using meso- tetraphenylporphyrin （TPP） and Ce（NO3）.6H20 in mixture solution of CHC13 and C2HsOH （V：V=1：1）. The complex was characterized by UV-Vis, FT-IR, conventional fluorescence, MALDI-TOF-MS, and ^1H NMR spectral techniques. The structure of complex was proposed viaSpectral analyses, in which tetraphenylporphyrin was coordinated to a cerium ion in a tetradentate fashion, while one nitrate was coordinated to the same cerium ion. After bubbling NO to the solution of Ce（TPP）NO3 in CH2Cl2, spectral analyses suggested that Ce（TPP）NO3 could interact with NO to form a novel complex of Ce（TPP）（NO）NO3, and NO was coordinated to the center cerium ion. When nitrogen was poured into the Ce（TPP）（NO）（NO3） solution, the complex could be reduced to Ce（TPP）NO3.展开更多
文摘表面增强激光解吸电离飞行时间质谱(Surface-enhanced laser desorption/ionization-time of flight,SELDI-TOF-MS)技术是目前蛋白质组学研究较为有效的手段之一,已被广泛的应用于肿瘤、传染性疾病、心血管病和神经系统等疾病的研究.然而SELDI-TOF-MS技术本身存在着一些问题,如分析结果有效性差和实验重复验证率低等,使其不能完全的应用于常规的临床诊断,现有许多研究致力于解决这些问题,如规范化实验室操作、预处理原始标本和数据、提高统计学方法等极大的改善和优化了这项技术.
文摘To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni- cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry （UPLC/Q-TOF- MS） and discuss their fragmentation regularity, the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data, tandem characteristic fragment ions and standards. In the end, 12 alkaloids were identi- fied from Ranunculus japonicus for the first time, and their fragmentation regularity was discussed. Thus, a rapid and effec- tive analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established.
文摘煤矿井下人员定位系统种类千变万化,但主要是在硬件和软件上的差异,因此,选择合适的硬件结构和软件架构会有效地避免井下巷道复杂,环境恶劣,干扰严重等因素.本文设计的基于NXP最新推出的集成片上TOF引擎的JN5168无线微控制器的井下人员定位前端控制系统,支持IEEE802.15.4无线通信协议,只需要设计芯片正常工作必要的外围电路,再通过改进TOF(Time of Flight)测距软件定位算法,便可构成一个精确定位系统.该系统满足成本低、开发时间短、定位误差低、功耗低、实时性快等优点,对煤矿井下复杂环境的人员定位系统的研制具有一定的理论和应用价值.
文摘物体表面积是物体的基本物理参数,在大学物理实验中有着广泛的应用。然而复杂物体的表面积测量是非常有难度的工作。为实现复杂不规则物体表面积的快速测量,采用脉冲激光TOF(Time Of Flight)技术采集待测物体的三维点云信息,然后对点云数据进行处理来实现三维模型的重建,最终实现模型的优化以及表面积数据的快速测量。实验结果表明系统能在较短的时间内实现模型重建并快速有效地实现表面积测量。这样的测量方法与传统的测量方法相比具有极大的创新性,系统成本低、不仅操作方便而且简单直观。其新颖性更是大大激发了大学生物理实验的学习兴趣。
文摘介绍一种基于3D成像的车载客流计数系统,通过飞行时间(Time of Flight,TOF)方式成像,实现对人员、物体、行李的识别。该系统可在每一个车门处对上下车人数进行准确统计,并将统计全车结果实时上传到服务器提供给其他系统使用,实现人员数量的准确检测。实验表明,该系统检测准确率高达95%以上,满足业主的需求。
基金ACKNOWLEDGMENTS This work was supported by the ＂Western Light＂ Visiting Scholar Plan, the Program for New Century Excellent Talents in University （No.NCET-12-1017）, the Program for Grassland Excellent Talents of Inner Mongolia Autonomous Region, the Inner Mengolia Science Technology Plan, the Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region （No.NJYT-12-B13）, the Natural Science Foundation of Inner Mongolia Autonomous Region （No.2011BS0601, China）, the Inner Mongolia Talented People Development Fund, and Yongfeng Boyuan Industry Co., Ltd. Jiangxi Province, China.
文摘Cerium （III） tetraphenylporphyrin nitrate Ce（TPP）NO3 was synthesized by using meso- tetraphenylporphyrin （TPP） and Ce（NO3）.6H20 in mixture solution of CHC13 and C2HsOH （V：V=1：1）. The complex was characterized by UV-Vis, FT-IR, conventional fluorescence, MALDI-TOF-MS, and ^1H NMR spectral techniques. The structure of complex was proposed viaSpectral analyses, in which tetraphenylporphyrin was coordinated to a cerium ion in a tetradentate fashion, while one nitrate was coordinated to the same cerium ion. After bubbling NO to the solution of Ce（TPP）NO3 in CH2Cl2, spectral analyses suggested that Ce（TPP）NO3 could interact with NO to form a novel complex of Ce（TPP）（NO）NO3, and NO was coordinated to the center cerium ion. When nitrogen was poured into the Ce（TPP）（NO）（NO3） solution, the complex could be reduced to Ce（TPP）NO3.
