[ Objective] The paper was explore the control effects of combination of plant induced resistant agents against tobacco mosaic vires （TMV）. [ Method ] The control effects of 6 different combinations of plant induced...[ Objective] The paper was explore the control effects of combination of plant induced resistant agents against tobacco mosaic vires （TMV）. [ Method ] The control effects of 6 different combinations of plant induced resistant agents against TMV of flue-cured tobacco cultivar HangDa were studied under the environ- ment of simulated disease nursery. [ Result] The combination of 2 induced agents polypeptide-agent and 3-acetonyl-3-hydroxyoxindole （AHO） had good control effect against TMV, which could obviously delay the incidence time of TMV in infected tobacco plants. With water and Duxiao as control, their average control effects against TMV of tobacco plants during field period reached 69.64% and 43.25% after transplanting for 70 d. They also showed significant superiority accord- ing to Duncan＇s test （p = 0.05 ） in the aspects of plant height and leaf number, and the growth and development condition of leaves was good. Tobacco seedlings carrying TMV virus had no direct correlation with whether the symptoms performed, the seedlings carrying virus would perform symptom only when the incidence condition was suitable. The peak period for the incidence of TMV in seedlings carrying virus was during 19 d after transplanting. Spraying effective agents during nursery stage and field period, as well as promoting quick growth at the initial stage of tobacco seedlings after transplanting were the key measures to control its inci- dence. [ Conclusion] The study provided theoretical basis for preparing the control measures against TMV.展开更多
[ Objectives] The paper was to study effects of different microbial agents on occurrence of tobacco bacterial wilt. [ Methods I Antagonistic strains against Ralstonia solanacearum were screened from in situ soil, and ...[ Objectives] The paper was to study effects of different microbial agents on occurrence of tobacco bacterial wilt. [ Methods I Antagonistic strains against Ralstonia solanacearum were screened from in situ soil, and prepared into four different combinations. The number of soil microorganisms and incidence of bacterial wilt of four combinations were observed. [ Results] The incidence rate of bacterial wilt treated by microbial agent OR-1 was significantly lower than that in control, and the control efficacy against bacterial wilt reached 67%. [ Conclusions] Application of microbial agent OR-1 could effectively reduce the incidence rate and disease index of bacterial wilt, thus improving the quality of tobacco.展开更多
The tobacco Ralstonia Solanacearum were both cultured on nutrient agar plates and inoculated in seedling stage of tobacco, then treated with K1 and K2, two anti-bacterial agents, at a serial con-centrations to study t...The tobacco Ralstonia Solanacearum were both cultured on nutrient agar plates and inoculated in seedling stage of tobacco, then treated with K1 and K2, two anti-bacterial agents, at a serial con-centrations to study their inhibitory efficiency. The result indicated that K1 can inhibit R. Solanacearum growth entirely, at the concentration range from 1/50 to 1/5000. K2 can reach the same result at the concentration range from 1/50 to 1/50000. Compared with the control plates, K1, at the concentration 1/50000, had no significant differences, and the average number of colony per plate was 112-115. The immature tobacco shown wilt as soon as inoculated with R. Solanacearum, and recovered gradually after using K1, K2. The densities of microbial suspension, handled by K1, K2 within 10 hs, were both significantly lower than the controlled ones. The optical microscopy also shown that handled microbial body differed from the controlled, whose body was regular short, rod shape as opposed to the handled ones with irregular rod shape and damaged body. All the results indicated that K1 and K2 both had inhibitory effects on tobacco R. Solanacearum, and K2 was more efficient than K1.展开更多
基金Supported by Key Project of China National Tobacco Company(110200801017)Co-funded Project of Yunnan Tobacco Company KunmingBranch(2010076)~~
文摘[ Objective] The paper was explore the control effects of combination of plant induced resistant agents against tobacco mosaic vires （TMV）. [ Method ] The control effects of 6 different combinations of plant induced resistant agents against TMV of flue-cured tobacco cultivar HangDa were studied under the environ- ment of simulated disease nursery. [ Result] The combination of 2 induced agents polypeptide-agent and 3-acetonyl-3-hydroxyoxindole （AHO） had good control effect against TMV, which could obviously delay the incidence time of TMV in infected tobacco plants. With water and Duxiao as control, their average control effects against TMV of tobacco plants during field period reached 69.64% and 43.25% after transplanting for 70 d. They also showed significant superiority accord- ing to Duncan＇s test （p = 0.05 ） in the aspects of plant height and leaf number, and the growth and development condition of leaves was good. Tobacco seedlings carrying TMV virus had no direct correlation with whether the symptoms performed, the seedlings carrying virus would perform symptom only when the incidence condition was suitable. The peak period for the incidence of TMV in seedlings carrying virus was during 19 d after transplanting. Spraying effective agents during nursery stage and field period, as well as promoting quick growth at the initial stage of tobacco seedlings after transplanting were the key measures to control its inci- dence. [ Conclusion] The study provided theoretical basis for preparing the control measures against TMV.
基金Supported by Science and Technology Project of Nanping Branch of Fujian Tobacco Company(NYK2012-14-3)
文摘[ Objectives] The paper was to study effects of different microbial agents on occurrence of tobacco bacterial wilt. [ Methods I Antagonistic strains against Ralstonia solanacearum were screened from in situ soil, and prepared into four different combinations. The number of soil microorganisms and incidence of bacterial wilt of four combinations were observed. [ Results] The incidence rate of bacterial wilt treated by microbial agent OR-1 was significantly lower than that in control, and the control efficacy against bacterial wilt reached 67%. [ Conclusions] Application of microbial agent OR-1 could effectively reduce the incidence rate and disease index of bacterial wilt, thus improving the quality of tobacco.
文摘The tobacco Ralstonia Solanacearum were both cultured on nutrient agar plates and inoculated in seedling stage of tobacco, then treated with K1 and K2, two anti-bacterial agents, at a serial con-centrations to study their inhibitory efficiency. The result indicated that K1 can inhibit R. Solanacearum growth entirely, at the concentration range from 1/50 to 1/5000. K2 can reach the same result at the concentration range from 1/50 to 1/50000. Compared with the control plates, K1, at the concentration 1/50000, had no significant differences, and the average number of colony per plate was 112-115. The immature tobacco shown wilt as soon as inoculated with R. Solanacearum, and recovered gradually after using K1, K2. The densities of microbial suspension, handled by K1, K2 within 10 hs, were both significantly lower than the controlled ones. The optical microscopy also shown that handled microbial body differed from the controlled, whose body was regular short, rod shape as opposed to the handled ones with irregular rod shape and damaged body. All the results indicated that K1 and K2 both had inhibitory effects on tobacco R. Solanacearum, and K2 was more efficient than K1.
