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Studies on Amylase and Degradation of Starch and Pigment of Tobacco Leaf During Process of Flue-Curing 被引量:17
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作者 GONGChang-rong YUANHong-tao +1 位作者 CHENJiang-hua SONGZhao-peng 《Agricultural Sciences in China》 CAS CSCD 2004年第12期931-936,共6页
The changes in the activity of amylase and amylase-isoenzyme and the degradation of starch and pigment of tobacco leaf during flue-curing were studied by using the electric- heated flue-curing barn designed and made... The changes in the activity of amylase and amylase-isoenzyme and the degradation of starch and pigment of tobacco leaf during flue-curing were studied by using the electric- heated flue-curing barn designed and made by the Henan Agricultural University. The temperature and humidity of the barn were controlled automatically. The results indicated that starch in tobacco leaf decreased rapidly and leveled off after 48 h of curring, in the meantime, the content of soluble sugar increased accordingly and reached a peak at the stage of color-fixing. Both of them had a rapid-changing stage in the first 36 hours of yellowing. The changes of starch and soluble sugar contents had highly significant negative-correlation at 1 % level (rNC89 = -0.8962**, rYY85 = -0.9704**). The activity of amylase increased with the proceeding of curing and reached a peak after 36 hours of curing, then decreased. But the activity of amylase kept at a high level when the humidity of curing-environment was very low, even if the tobacco leaf had been dried. The rapid degradation of starch showed a significantly negative correlation with the increase of activity of amylase at 5 % level (rNC89 = -0.8495*, rYY85 = -0.7839*). The degradation of starch and pigment had the same regulation and had highly significant correlation at 1 % level (rNC89= 0.9649**, rYY85= 0.9428**). There were mainly three amylase-isoenzyme bands -A, B, C respectively, in tobacco leaf during flue curing. They were identified as α-AMY, β-AMY, R-AMY, and the activity of β-AMY was the highest. The changes in amylase activity and contents of starch and pigment were affected by the tobacco leaf moisture and environmental humidity during curing. 展开更多
关键词 tobacco leaf Flue-curing STARCH AMYLASE PIGMENT
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Dynamics of Environmental Humidity and Water Content in Tobacco Leaf and Metabolism of Starch During Curing 被引量:1
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作者 GONGChang-rong CHENJiang-hua 《Agricultural Sciences in China》 CAS CSCD 2003年第2期149-153,共5页
Effect of environmental humidity and water content in tobacco leaf on starch metabolism was studied by using the electric-heated auto-controlled flue-curing barn supplied by Henan Agricultural University, China. The r... Effect of environmental humidity and water content in tobacco leaf on starch metabolism was studied by using the electric-heated auto-controlled flue-curing barn supplied by Henan Agricultural University, China. The results indicated that the degradation of starch and decrease of water content in tobacco leaf during early yellowing at low humidity was the most rapid, and the water loss was the highest while the lowest under high humidity. The duration for starch degradation under low humidity was longer than that of CK. So the starch residue in cured leaf of different treatment took the order of high humidity yellowing treatment> low humidity yellowing treatment > CK. When the leaf water content was decreased to around 50 %, the starch degradation became slow and the content of starch was stable. Starch degradation and decrease of leaf water content was not synchronous. Starch in tobacco leaf during yellowing degraded more rapidly when humidity was decreased at a high speed, but the degradation stopped earlier at late stage. There was a quicker and higher degradation of starch under high environmental humidity. When the humidity decreased to 70 %, the content of starch was stable. The activity of amylase began to decrease when relative humidity was below 75 %, but it kept a high level of activity when the environmental humidity was below 70 %. 展开更多
关键词 tobacco leaf Flue-curing STARCH Relative humidity AMYLASE
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Occurrence Regularity and Life History of Cigarette Beetle Lasioderrma serricorne(Fabricius)in Tobacco Leaf Threshing and Redrying Workshop
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作者 Ding CHEN Yingjie JIANG +9 位作者 Zijun ZHAO Chaojun LUO Xingling WANG Jie XIE Pengchao CHEN Fei XU Yongan REN Lin LU Xueqin ZHANG Shunxian LIANG 《Plant Diseases and Pests》 CAS 2022年第3期1-4,共4页
[Objectives]The paper was to study the occurrence regularity and life history of cigarette beetle Lasioderrma serricorne(Fabricius)in tobacco leaf threshing and redrying workshop.[Methods]With the tobacco leaf threshi... [Objectives]The paper was to study the occurrence regularity and life history of cigarette beetle Lasioderrma serricorne(Fabricius)in tobacco leaf threshing and redrying workshop.[Methods]With the tobacco leaf threshing and redrying workshop as the survey site,the occurrence regularity of L.serricorne was investigated,and the life history table was inferred according to its biological characteristics.The temperature,humidity and duration of tobacco leaf threshing and redrying were simulated in the laboratory to study the survival rate of all states of L.serricorne in this environment.[Results]The lifecycle of L.serricorne in tobacco leaf threshing and redrying workshop was:the overwintering generation pupated in late February;the pupa began to emerge in early March,and reached its peak in late March.The first-generation eggs started hatching in late March,pupated in late May,emerged in early June,and reached the peak eclosion of the first-generation adults in mid-July.The second-generation eggs began to hatch in late July,pupated in mid-August,emerged in late August,and reached the peak eclosion of the second-generation adults in mid-September.The third-generation eggs began to hatch in mid-September;most of the larvae began to overwinter,some pupated in mid-November and survived the winter as pupae,and some pupae emerged to adults.The survival test results of different states of L.serricorne under simulated temperature,humidity and duration in the tobacco leaf threshing and redrying process showed that the mortality rates of eggs in simulated 1,2 and 3 conditions were about 51.22%,90.24% and 100%,and the mortalities of larvae in simulated 1,2 and 3 conditions were about 18.30%,81.25%and 100%,respectively.The mortalities of pupae in simulated 1,2 and 3 conditions were about 69.39%,100% and 100%,and the mortalities of adults in simulated 1,2 and 3 conditions were about 100%,100% and 100%,respectively.[Conclusions]L.serricorne of different states can be killed by appropriately raising the temperature during threshing and redrying. 展开更多
关键词 Cigarette beetle LIFECYCLE tobacco leaf threshing and redrying workshop Mortality rate REGULARITY
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Low Co-Cultivation Temperature at 20°C Resulted in the Reproducible Maximum Increase in Both the Fresh Weight Yield and Stable Expression of GUS Activity after <i>Agrobacterium tumefaciens</i>-Mediated Transformation of Tobacco Leaf Disks 被引量:3
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作者 Guiying Su Sunjung Park +1 位作者 Seokhyun Lee Norimoto Murai 《American Journal of Plant Sciences》 2012年第4期537-545,共9页
The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Ni... The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks. 展开更多
关键词 Agrobacterium TUMEFACIENS CO-CULTIVATION TEMPERATURE Fresh Weight Yield Stable GUS Gene Expression tobacco leaf DISKS Transformation
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How to Comprehensively Utilize the Discarded Fresh Tobacco Leaf ?
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作者 Xiqing LIU Weiping FENG +3 位作者 Zhifu XU Dongyang HU Zairan DENG Wenyu LI 《Asian Agricultural Research》 2015年第12期74-76,共3页
This paper aims to explore comprehensive utilization way of discarded fresh tobacco leaf. Based on modern tobacco demands on green,environmental protection and sustainable development,as well as actual situation of bi... This paper aims to explore comprehensive utilization way of discarded fresh tobacco leaf. Based on modern tobacco demands on green,environmental protection and sustainable development,as well as actual situation of biogas production facility in some villages of local tobacco area,this paper selects Longwan Village of Fengshiyan Town as the experimental site and explores the rational way of discarded fresh tobacco leaf biogas fermentation. Results show that cutting and fermentation of discarded fresh tobacco leaf before adding into biogas digester is conductive to improving p H in fermentation process,reducing crust amount,and effectively improving biogas production amount and rate. The study can provide reference for effective processing and rational utilization of discarded fresh tobacco leaf. 展开更多
关键词 Discarded FRESH tobacco leaf BIOGAS FERMENTATION COMPREHENSIVE utilization
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Identification of a novel DNA molecule associated with Tobacco leaf curl virus 被引量:6
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作者 XIE Yan ZHOU Xueping +2 位作者 LI Zhenghe ZHANG Zhongkai LI Guixin 《Chinese Science Bulletin》 SCIE EI CAS 2002年第15期1273-1276,共4页
A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp... A circular DNA molecule, designated as DNAp, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAp sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAP consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβhas little sequence homology to DNA-A of TLCV, except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%-65%) with the reported DNAβ molecules associated with Agera-tum yellow vein virus and Cotton leaf 展开更多
关键词 DNAp tobacco leaf CURL VIRUS sequence BEGOMOVIRUS Bemisa tabaci.
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Preliminary Study on Molecular Evolution of Tobacco Leaf Curl Geminivirus 被引量:2
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作者 洪益国 蔡健和 +3 位作者 王小凤 王文慧 黄谊 田波 《Chinese Science Bulletin》 SCIE EI CAS 1994年第6期520-523,共4页
Leaf curl is a serious disease of tobacco in tropical and subropical regions. Itscausative agent is tobacco leaf curl geminivirus (TLCV)which can be transmitted bywhitefly (Bemisia tabaci) and infect many other plant ... Leaf curl is a serious disease of tobacco in tropical and subropical regions. Itscausative agent is tobacco leaf curl geminivirus (TLCV)which can be transmitted bywhitefly (Bemisia tabaci) and infect many other plant species of Solanaceae andCaricaceae.The occurrence, host range and insect vector of TbLCV as well as itsserological reaction with monoclonal antibody of African cassava mosaic virus havebeen reported in China, and more recently, tobacco leaf curl disease has becomeendemic in southern areas. Here we first present the nucleotide sequence ofTbLCV coat protein (CP) gene and molecular evolutionary relationship with 展开更多
关键词 tobacco leaf CURL GEMINIVIRUS COAT protein gene molecular evolution.
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Effects of plastic mulching film-induced leaf burning on seedling growth in tobacco cultivation:Different findings beyond conservation view 被引量:2
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作者 LIN Ying-chao WEI Ke-su +5 位作者 GAO Wei-chang CHEN Yi LIN Ye-chun CHEN Wei LI Hong-xun PAN Wen-jie 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第6期1327-1337,共11页
Solving high-temperature plastic mulching film-induced leaf burning in the first week during tobacco cultivation would take much time and effort. In the present study, the growth as well as the leaf sugar and nicotine... Solving high-temperature plastic mulching film-induced leaf burning in the first week during tobacco cultivation would take much time and effort. In the present study, the growth as well as the leaf sugar and nicotine contents of seedlings with or without leaf burning induced by high-temperature plastic mulching film were tested at two independent sites in 2015 and 2016 to identify the influence of leaf burning on seedling growth. The results showed that the growth of seedlings with leaf burning was improved with increased leaf area, leaf number and plant height compared to those without leaf burning, combined with an increased seedling survival rate at two sites in two years. In seedlings with leaf burning, the contents of fructose and glucose increased and peaked at 11:00 and 13:00 in the leaf and root, respectively, with an increased root nicotine content beginning at 13:00, highlighting the signalling role of sugars. Activities of antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were all increased in seedlings with leaf burning. More plant biomass was allocated to roots in seedlings with leaf burning with increased root volume compared to control seedlings, which might facilitate the absorption of water and nutrients from the soil. Our findings demonstrate that high-temperature plastic mulching film-induced leaf burning not inhibited but benefited seedling survival and growth, suggesting that the time and labour-consuming manual plucking of burnt leaves can be avoided during tobacco cultivation. 展开更多
关键词 plastic mulching film tobacco cultivation leaf burning NICOTINE
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There is the second virus that causes tobacco leaf curl disease (not TbLCV-CHI) in the field
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作者 GONG Qianhong LIU Yule +3 位作者 HONG Yiguo WANG Huanyu CHEN Gang CAI Jianhe 《Chinese Science Bulletin》 SCIE EI CAS 2000年第12期1131-1137,共7页
Sequence analysis of virus isolation DNA of tobacco leaf curl disease shows that there is the second geminivirus (not Chinese Tobacco Leaf Curl Virus, TbLCV-CHI) that causes tobacco leaf curl disease in the field in t... Sequence analysis of virus isolation DNA of tobacco leaf curl disease shows that there is the second geminivirus (not Chinese Tobacco Leaf Curl Virus, TbLCV-CHI) that causes tobacco leaf curl disease in the field in the Guangxi Zhuang Autonomous Region, China. This virus DNA-A contains 2 734 nt. Large intergenic region (LIR) contains 269 nt, the virus sense strand contains 2 open reading frames (ORFs): AV1 (115 aa) and AV2 (coat protein gene, CP, 256 aa), and the complementary sense strand contains 4 ORFs: AC1 (replicase gene, 361 aa), AC2 (transactivator, 134 aa), ACS (134 aa) and AC4 (97 aa). The virus belongs to one kind of subgroup III gemini- viruses from old world, and could be the Chinese tomato yellow leaf curl virus (TYLCV-CHI). 展开更多
关键词 tobacco leaf CURL DISEASE geminivirus DNA sequence CHINESE tomato yellow leaf CURL VIRUS (TYLCV-CHI) CHINESE tobacco leaf CURL VIRUS (TbLCV-CHI).
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基于YOLOv5的烟草叶部病害智能识别
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作者 刘春菊 刘延鑫 +11 位作者 李斐 王俊峰 刘中庆 聂威 王大海 刘洋 田海东 罗政刚 孙松 杜玉海 马强 姜红花 《中国烟草科学》 CSCD 北大核心 2024年第3期93-101,共9页
为提高烟草病害的智能识别精度和判别效率,提出基于YOLOv5网络改进的烟草病害识别模型,针对原模型对小目标病斑特征提取能力弱的问题提出改进模型,分别为添加多尺度增强模块的YOLOv5-ME、添加小目标检测层的YOLOv5-LT和同时添加小目标... 为提高烟草病害的智能识别精度和判别效率,提出基于YOLOv5网络改进的烟草病害识别模型,针对原模型对小目标病斑特征提取能力弱的问题提出改进模型,分别为添加多尺度增强模块的YOLOv5-ME、添加小目标检测层的YOLOv5-LT和同时添加小目标检测层和多尺度增强模块的YOLOv5-ME-LT,对从田间采集的赤星病、黄瓜花叶病、普通花叶病、气候斑点病和野火病等5874幅病害图像进行识别验证。结果表明,3种改进模型的检测精度均优于原始模型,YOLOv5-ME的mAP为88.7%;YOLOv5-LT的mAP为88.1%;YOLOv5-ME-LT的mAP为91%,远高于原模型的78%。本文改进的烟草叶部病害识别算法相对原模型性能有明显提高,但研究中仍存在相似病害难区分等问题,后续研究将结合生命化学指标和多光谱技术,对早期烟草病害进行检测。 展开更多
关键词 烟草 叶部病害 检测 YOLOv5 深度学习
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微波预处理对加热卷烟用烤烟烟叶常规化学成分及挥发性香气成分的影响
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作者 田金虎 袁颖 +5 位作者 卢昕博 汤晓东 潘力 王雨凝 叶兴乾 蒋健 《轻工学报》 CAS 北大核心 2024年第2期69-79,共11页
以河南产区的下部烤烟样品为原料,采用微波干燥和微波水热两种方法预处理原料,利用连续流动分析、高效液相色谱、气相色谱-离子迁移谱(GC-IMS)等技术对不同微波预处理烤烟烟叶中的常规化学成分及挥发性香气成分进行分析。结果表明:微波... 以河南产区的下部烤烟样品为原料,采用微波干燥和微波水热两种方法预处理原料,利用连续流动分析、高效液相色谱、气相色谱-离子迁移谱(GC-IMS)等技术对不同微波预处理烤烟烟叶中的常规化学成分及挥发性香气成分进行分析。结果表明:微波水热处理可降低烤烟烟叶中水溶性糖、还原糖、植物碱及酚类物质含量,提高总氮、石油醚提取物含量,并显著增加烤烟原叶的氮碱比(P≤0.05),其中微波水热700 W-6 min处理组氮碱比高达1.65;而经微波干燥处理后,烤烟烟叶中常规化学成分整体仍处于优质烟叶指标范畴,且挥发碱含量显著增加(P≤0.05),部分预处理组中绿原酸和芸香苷含量增加;此外,GC-IMS共鉴定出56种挥发性香气成分,微波水热处理可增加烤烟的新鲜清香,微波干燥处理则可增加其甜香味。 展开更多
关键词 微波水热处理 微波干燥处理 烤烟烟叶 加热卷烟 挥发性香气成分
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不同叶长·叶宽和单叶重对广元烤后烟叶质量的影响
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作者 石刚 王晶 +6 位作者 王鹏 叶协锋 刘腾飞 吴勇 蒲飞 吴明 张波 《安徽农业科学》 CAS 2024年第14期156-160,共5页
为明确广元优质烤后烟叶适宜的叶长、叶宽和单叶重范围,为广元地区生产优质烤烟提供数据支撑,2021年在四川省广元市剑阁县5个收购线分别挑选10户种植水平具有代表性的烟农,取X2F、C2F、C3F和B2F等级烟叶样品各2 kg,按等级划分不同叶长... 为明确广元优质烤后烟叶适宜的叶长、叶宽和单叶重范围,为广元地区生产优质烤烟提供数据支撑,2021年在四川省广元市剑阁县5个收购线分别挑选10户种植水平具有代表性的烟农,取X2F、C2F、C3F和B2F等级烟叶样品各2 kg,按等级划分不同叶长、叶宽和单叶重进行烟叶质量评价。结果表明:随着叶长的增加,X2F、B2F等级烟叶总糖、还原糖含量呈减少趋势。随着叶长的增加,C3F烟叶烟碱、钾含量升高;随着叶宽的增加,C3F烟叶总糖、还原糖含量逐渐增加。X2F和C3F等级烟叶随着单叶重的增加,总糖含量逐渐增加,钾含量减少。随着烟叶叶宽和单叶重的增加,X2F烟叶外观质量总分均表现为先增加后降低。随着叶长的增加,C2F和C3F烟叶外观质量总分均表现为逐渐增加,外观质量总分从高到低依次为>75 cm、>65~75 cm、>55~65 cm、≤55 cm。随着叶长、叶宽和单叶重的增加,C2F、C3F、B2F烟叶感官质量得分大多表现为先上升后下降。随着叶长、叶宽的增加,C2F、B2F烟叶感官质量得分表现为先上升后下降。当X2F烟叶叶长控制在>50~60 cm、叶宽控制在>15~19 cm、单叶重控制在>7~11 g时,X2F烟叶综合品质较好;当C2F烟叶叶长控制在>55~65 cm、叶宽控制在>15~23 cm、单叶重控制在>9~13 g时,C2F烟叶综合品质较好;当C3F烟叶叶长控制在>65~75 cm、叶宽控制在>15~23 cm、单叶重控制在>8~12 g时,C3F烟叶综合品质较好;当B2F叶长控制在>55~65 cm、叶宽控制在>15~23 cm、单叶重控制在>9~15 g时,B2F烟叶综合品质较好。 展开更多
关键词 烤后烟叶 叶长 叶宽 单叶重 烟叶质量
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不同尺寸片烟游离氨基酸和阿马杜里化合物差异分析
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作者 陈红丽 周航 +4 位作者 杨永锋 胡静宜 刘向真 刘茂林 贾涛 《西北农林科技大学学报(自然科学版)》 CSCD 北大核心 2024年第8期60-69,共10页
【目的】明确打叶复烤后不同尺寸片烟游离氨基酸和阿马杜里(Amadori)化合物的变化规律,为稳定卷烟质量和调整配方提供理论依据。【方法】以河南中烟工业有限责任公司6个中间香型烤烟多等级配打模块打叶后的片烟为研究对象,经叶片振动分... 【目的】明确打叶复烤后不同尺寸片烟游离氨基酸和阿马杜里(Amadori)化合物的变化规律,为稳定卷烟质量和调整配方提供理论依据。【方法】以河南中烟工业有限责任公司6个中间香型烤烟多等级配打模块打叶后的片烟为研究对象,经叶片振动分选筛筛分后得到大片、中片、小片、碎片和碎末5种不同尺寸的片烟,分别测定其游离氨基酸和阿马杜里化合物含量,并对其分别进行差异显著性分析、主成分分析和聚类分析。【结果】(1)不同尺寸片烟21种游离氨基酸总量为14 586.82~18 614.04μg/g,其中脯氨酸、天冬酰胺、丙氨酸和天冬氨酸含量较高;不同尺寸片烟17种阿马杜里化合物总量为21 674.68~29 371.17μg/g,其中果糖-脯氨酸(Fru-Pro)、果糖-丙氨酸(Fru-Ala)、果糖-氨基丁酸(Fru-Amb)和果糖-天冬酰胺(Fru-Asn)含量较高;不同尺寸片烟在多个游离氨基酸组分、阿马杜里化合物组分及阿马杜里化合物总量上存在显著差异。(2)主成分分析表明,游离氨基酸前3个主成分的累积方差贡献率为89.89%,表明这3个主成分可以反映21个变量(游离氨基酸成分)的大部分信息;阿马杜里化合物前4个主成分的累积方差贡献率为88.95%,表明这4个主成分能够反映17个变量(阿马杜里化合物)的大部分信息。(3)聚类分析结果表明,根据游离氨基酸主成分综合得分,可将不同尺寸片烟聚为3类,其中大片、中片和小片烟聚为一类;碎片烟和碎末烟各自单独聚为一类;根据阿马杜里化合物主成分综合得分,可将不同尺寸片烟聚为2类,其中大片、中片和小片烟聚为一类;碎片烟和碎末烟聚为一类。【结论】大片片烟和中片片烟具有较好的品质,工业可用性较高;碎片烟和碎末烟品质较差,在打叶复烤时应尽量减少其产出。 展开更多
关键词 片烟尺寸 游离氨基酸 阿马杜里化合物 烟叶加工
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施用侧孢短芽孢杆菌对烤后烟叶钾含量及烟株生理特征的影响
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作者 宋鹏 李理想 +7 位作者 江厚龙 王茹 李慧 赵鹏宇 张均 秦平伟 任江波 陈庆明 《浙江农业学报》 CSCD 北大核心 2024年第3期494-502,共9页
为探究施用侧孢短芽孢杆菌(Brevibacillus laterosporus)对烤后烟叶钾含量及烟株生理特征的影响,在烟苗移栽期设置不施用侧孢短芽孢杆菌(T1)、侧孢短芽孢杆菌浸根(T2)、侧孢短芽孢杆菌灌根(T3)及侧孢短芽孢杆菌浸根+灌根(T4)4个处理,测... 为探究施用侧孢短芽孢杆菌(Brevibacillus laterosporus)对烤后烟叶钾含量及烟株生理特征的影响,在烟苗移栽期设置不施用侧孢短芽孢杆菌(T1)、侧孢短芽孢杆菌浸根(T2)、侧孢短芽孢杆菌灌根(T3)及侧孢短芽孢杆菌浸根+灌根(T4)4个处理,测定烤后烟叶钾含量、叶片光合色素含量、抗氧化酶活性及抗病性等指标的变化。结果表明:侧孢短芽孢杆菌的最佳施用方式为浸根+灌根,该处理烤后上部及中部叶的钾含量较T1处理分别显著(P<0.05)提高29.86%、67.36%,根系钾含量显著提高16.58%~28.85%,烟株气候斑病、炭疽病、赤星率、叶斑病的发病率和病情指数显著降低,烟叶的叶绿素、类胡萝卜素含量,及超氧化物歧化酶、过氧化物酶活性分别显著提高13.21%~18.81%、21.01%~35.41%、9.63%~18.31%、11.62%~13.81%,叶片丙二醛含量显著降低20.76%~30.99%。综上,在烟苗移栽期以浸根+灌根方式施用侧孢短芽孢杆菌可增强烟株的抗性,提高烤后烟叶的钾含量。 展开更多
关键词 侧孢短芽孢杆菌 烤后烟叶 抗氧化酶活性 钾含量 抗病性
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多感受野特征自适应融合及动态损失调整的初烤烟叶等级检测
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作者 何自芬 罗洋 +3 位作者 张印辉 陈光晨 陈东东 徐林 《光学精密工程》 EI CAS CSCD 北大核心 2024年第2期301-316,共16页
初烤烟叶等级的快速准确检测对开发烟叶智能分级设备以促进农产品精细化管理有着重要意义。针对相似度较高但等级不同的初烤烟叶难以区分的问题,本文提出多感受野特征自适应融合及动态损失调整的初烤烟叶等级检测网络(Flue-cured Tobacc... 初烤烟叶等级的快速准确检测对开发烟叶智能分级设备以促进农产品精细化管理有着重要意义。针对相似度较高但等级不同的初烤烟叶难以区分的问题,本文提出多感受野特征自适应融合及动态损失调整的初烤烟叶等级检测网络(Flue-cured Tobacco Leaf Grade Detection Network,FTGDNet)。首先,FTGDNet采用CSPNet作为特征提取主干网络,采用GhostNet作为辅助特征提取网络以增强模型的特征提取能力;其次,在主干网络末端嵌入显式视觉中心瓶颈模块(Explicit Visual Center Bottleneck module,EVCB)以实现全局特征信息与局部细节特征信息融合;然后,构建多感受野特征自适应融合模块(Multi-Receptive Field Feature Adaptive Fusion module,MRFA_d),利用注意力特征融合机制(Attention Feature Fusion,AFF)将不同感受野特征图进行自适应加权融合,在增强模型局部感受野的同时突出有效通道信息;最后,设计了一种新的定位损失函数(More Complete IoU Loss,MCIoU_Loss),结合预测框与真实框面积损失以解决在回归定位过程中二者宽高比相等且中心点重合时CIoU_Loss性能退化导致定位精度下降问题,此外,引入矩形相似度衰减系数在训练过程中对真实框与预测框的相似度判别项进行动态调整,加快模型拟合。实验结果表明,FTGDNet对十个等级的初烤烟叶的验证精度达到90.0%,测试精度达到87.4%,且推理时间仅为12.6 ms。相较于多种先进目标检测算法,FTGDNet具有更高的检测精度和更快的检测速度,可为高精度初烤烟叶等级检测提供关键技术支撑。 展开更多
关键词 初烤烟叶 目标检测 多感受野特征融合 动态损失调整
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生物有机肥与化肥减量配施对土壤养分及烟叶产质量的影响
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作者 曾祥难 李思 +8 位作者 袁彩云 杨祝军 欧义 刘永斌 彭博 余育光 范鹏 蔺万煌 韦建玉 《中国农学通报》 2024年第18期66-72,共7页
为减少化肥的过量施用,提高湖南宜章植烟土壤肥力和烟叶生产效益,以烤烟品种‘K326’为材料,采用田间试验研究了不同比例生物有机肥与化肥减量配施对土壤养分及烟叶产量与品质的影响。结果表明:增施适当比例生物有机肥配合化肥减量能不... 为减少化肥的过量施用,提高湖南宜章植烟土壤肥力和烟叶生产效益,以烤烟品种‘K326’为材料,采用田间试验研究了不同比例生物有机肥与化肥减量配施对土壤养分及烟叶产量与品质的影响。结果表明:增施适当比例生物有机肥配合化肥减量能不同程度地改善土壤肥力质量,促进烟株的生长。随着生物有机肥比例的增加与化肥用量的减少,烤烟的各项生长农艺性状、感官评吸质量、产量、产值以及上等烟比例呈先升后降的趋势。其中,以减量20%化学追肥的T2处理对烟株的生长和烟叶的品质表现最好。与常规施肥的对照(CK)相比,烟草株高、有效叶片数和最大叶面积分别增加8.24%~17.00%、13.81%~23.22%和12.63%~16.86%,C3F烟叶中总糖和钾含量分别达到26.51%~30.01%和2.16%~2.86%,C3F和B2F烟叶的感官评吸质量分别提高1.67%~2.98%和3.12%~4.10%,烟叶产量、上等烟比例和产值分别提高3.76%~6.58%、3.38%~3.79%和8.24%~9.58%。研究结果为湘南植烟区应用生物有机肥与化肥减量配施提供了科学参考依据。 展开更多
关键词 烤烟 生物有机肥 化肥减量增效 土壤养分 烟叶产质量
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基于单料烟的加热卷烟与传统卷烟香气成分释放差异分析
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作者 何红梅 尤晓娟 +5 位作者 王鸣 郭宏霞 郑晓云 徐如彦 石怀彬 饶先立 《轻工学报》 CAS 北大核心 2024年第3期99-108,共10页
将10种单料烟分别制备成加热卷烟和传统卷烟两种类型卷烟样品,采用GC-MS分析和正交偏最小二乘法判别分析(OPLS-DA)模型评价加热卷烟气溶胶和传统卷烟主流烟气中香气成分的释放差异。结果表明:加热卷烟气溶胶和传统卷烟主流烟气中分别鉴... 将10种单料烟分别制备成加热卷烟和传统卷烟两种类型卷烟样品,采用GC-MS分析和正交偏最小二乘法判别分析(OPLS-DA)模型评价加热卷烟气溶胶和传统卷烟主流烟气中香气成分的释放差异。结果表明:加热卷烟气溶胶和传统卷烟主流烟气中分别鉴定出53种和77种香气成分;两者共有香气成分47种,其中13种香气成分在加热卷烟气溶胶中的释放量较为突出,除乙酸、γ-丁内酯外,其他11种均是醛酮类化合物;筛选出两种卷烟中存在显著释放差异的香气成分53种,包含吡嗪吡啶类7种、酮类12种、呋喃类4种、酸类9种、酯类3种、酚类8种、烃类9种、醇类1种,从差异性香气成分在该类别总鉴定成分的占比来看,酚类香气成分在两种卷烟中的释放差异性最为显著,其余依次为烃类、吡嗪吡啶类、酸类、酯类、酮类、呋喃类、醇类。 展开更多
关键词 单料烟 加热卷烟 传统卷烟 香气成分 正交偏最小二乘法判别分析
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烤烟上部叶成熟度对烟叶品质的影响
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作者 王鹏 陈炬廷 +5 位作者 石刚 叶协锋 吴明 吴少军 付龙 吴勇 《现代农业科技》 2024年第8期13-16,共4页
为探究四川省广元产区上部烟叶适宜的采收成熟度,以云烟87为试验材料,设置4个成熟度处理,测定不同处理烤后烟外观质量、化学成分、感官质量及经济效益。结果表明:2021年较常规采收时间推迟3 d采收,上部烟叶烤后外观质量最好,化学成分较... 为探究四川省广元产区上部烟叶适宜的采收成熟度,以云烟87为试验材料,设置4个成熟度处理,测定不同处理烤后烟外观质量、化学成分、感官质量及经济效益。结果表明:2021年较常规采收时间推迟3 d采收,上部烟叶烤后外观质量最好,化学成分较为协调,但还原糖和总糖含量稍低于优质烟叶目标;2022年较常规采收时间推迟3 d采收,上部烟叶烤后外观质量、感官质量及经济效益均最好。综合来看,建议较常规采收时间推迟3 d,此时可达到广元烟区上部烟叶采收的最佳成熟度。 展开更多
关键词 上部烟叶 成熟度 外观质量 感官质量 经济效益
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山东省不同类型烟叶质量特征差异及原因分析
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作者 王永 孙延国 +3 位作者 王德权 戴华伟 王世建 吴会才 《现代农业科技》 2024年第10期141-150,共10页
为明确山东省不同类型烟叶质量特征差异及原因,本研究连续3年采集山东烟区烟叶样品,分析不同类型烟叶物理特性、化学成分和感官评吸质量,通过相关分析,解析影响评吸质量的主要因素。结果表明:中、上部叶物理特性方面,香吃味型最好,香味... 为明确山东省不同类型烟叶质量特征差异及原因,本研究连续3年采集山东烟区烟叶样品,分析不同类型烟叶物理特性、化学成分和感官评吸质量,通过相关分析,解析影响评吸质量的主要因素。结果表明:中、上部叶物理特性方面,香吃味型最好,香味型居其次,吃味型最低,表现在部分吃味型中、上部叶发育过旺,叶片大小、单叶重、叶面密度超出适宜范围。化学成分方面,香味型中部叶化学成分协调性最好,香吃味型中部叶居于其次,吃味型中部叶最低,主要是部分吃味型烟叶淀粉、氯离子含量高且钾氯比低;3种类型上部叶化学成分协调性基本一致。感官评吸质量方面,中部叶质量得分为香味型最高,香吃味型其次,吃味型最低,差异主要体现在香气质、余味、杂气、刺激性等指标;3种类型上部烟叶评吸质量差异不明显。相关分析表明,叶宽对中部叶评吸质量产生显著负效应,叶长、叶片厚度对上部叶评吸质量产生显著负效应;钾和钾氯比对中部叶评吸质量产生显著正效应,而还原糖、硫、氯离子、纤维素、半纤维素产生显著负效应;还原糖、总糖对上部叶评吸质量产生显著正效应,而烟碱、纤维素产生负效应。吃味型中部叶评吸质量较低,与其叶片发育过旺及硫、氯含量较高有关;3种类型上部叶评吸质量无显著差异,但其原因各有不同,香味型、香吃味型上部叶虽然钾含量高、氯离子含量低,但总糖含量低、烟碱含量高,糖碱比协调性变差,而吃味型上部叶钾含量低、氯离子含量高,总糖含量高、烟碱含量低,糖碱比协调。因此,需针对不同类型烟叶的问题,采取相应农艺措施,提高烟叶质量。 展开更多
关键词 烤烟 烟叶类型 物理特性 化学成分 感官质量 特征差异 山东省
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基于ADAMS的烟叶运输车自主校正机构仿真与试验
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作者 杨振纲 陈献勇 +2 位作者 郑仁富 钱勤智 彭宇峰 《福建农机》 2024年第2期18-22,共5页
丘陵山区烟田地形复杂,烟叶运输车在垄沟行驶时方向易发生偏离。针对福建丘陵烟区地形,通过对设计的烟叶运输车自主校正机构进行力学分析,并结合ADAMS仿真对比有自主校正机构与无自主校正机构两种情况下烟叶运输车在垄沟行驶的偏离程度... 丘陵山区烟田地形复杂,烟叶运输车在垄沟行驶时方向易发生偏离。针对福建丘陵烟区地形,通过对设计的烟叶运输车自主校正机构进行力学分析,并结合ADAMS仿真对比有自主校正机构与无自主校正机构两种情况下烟叶运输车在垄沟行驶的偏离程度,仿真结果表明:有自主校正机构的烟叶运输车比无自主校正机构烟叶运输车偏离程度小了10%。最后通过田间试验进一步验证了该机构发挥了自主校正的作用。该研究对丘陵烟田烟叶运输机械的研发具有一定的借鉴参考作用。 展开更多
关键词 烟叶运输 自主校正 ADAMS仿真 Origin数据分析
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