'产品的功能与情感因素是不可分割的,只有两者并存,才能实现创新。而形状、材料和色彩,都是用来创造功能和情感的元素。''Function and emotion are inseparable. Together they form the door to innovation.Form, materia...'产品的功能与情感因素是不可分割的,只有两者并存,才能实现创新。而形状、材料和色彩,都是用来创造功能和情感的元素。''Function and emotion are inseparable. Together they form the door to innovation.Form, material and color are the elements with which one creates that function and emotion.'德国时间3月15日晚.展开更多
Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use of these varie...Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use of these varieties. To overcome this limitation, we sought to develop an effective transformation system for hull-less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%, using a modified Agrobacterium transformation method. This method was used to over-express genes encoding synthases for the important dietary fiber component, 0,3;1,4）-β-glucan （mixed-linkage glucan）, primarily present in starchy endosperm cell walls. Over-expression of the HvCslF6 gene, driven by an endosperm-specific promoter, produced lines where mixed-linkage glucan content increased on average by 45%, peaking at 70% in some lines, with smaller increases in transgenic HvCsIH1 grain. Transgenic HvCslF6 lines displayed alterations where grain had a darker color, were more easily crushed than wild type and were smaller. This was associated with an enlarged cavity in the central endosperm and changes in cell morphology, including aleurone and sub-aleurone cells. This work provides proof-of-concept evidence that mixed-linkage glucan content in hull-less barley grain can be increased by over-expression of the HvCslF6 gene, but also indicates that hull-less cultivars may be more sensitive to attempts to modify cell wall composition.展开更多
文摘'产品的功能与情感因素是不可分割的,只有两者并存,才能实现创新。而形状、材料和色彩,都是用来创造功能和情感的元素。''Function and emotion are inseparable. Together they form the door to innovation.Form, material and color are the elements with which one creates that function and emotion.'德国时间3月15日晚.
基金supported by funding from the Adelaide Graduate Research Scholarship(AGRS)Australian Research Council Centre of Excellence in Plant Cell Wallsthe CSIRO Flagship Collaboration Fund
文摘Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use of these varieties. To overcome this limitation, we sought to develop an effective transformation system for hull-less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%, using a modified Agrobacterium transformation method. This method was used to over-express genes encoding synthases for the important dietary fiber component, 0,3;1,4）-β-glucan （mixed-linkage glucan）, primarily present in starchy endosperm cell walls. Over-expression of the HvCslF6 gene, driven by an endosperm-specific promoter, produced lines where mixed-linkage glucan content increased on average by 45%, peaking at 70% in some lines, with smaller increases in transgenic HvCsIH1 grain. Transgenic HvCslF6 lines displayed alterations where grain had a darker color, were more easily crushed than wild type and were smaller. This was associated with an enlarged cavity in the central endosperm and changes in cell morphology, including aleurone and sub-aleurone cells. This work provides proof-of-concept evidence that mixed-linkage glucan content in hull-less barley grain can be increased by over-expression of the HvCslF6 gene, but also indicates that hull-less cultivars may be more sensitive to attempts to modify cell wall composition.
