This study investigated the effects of propofol on the mRNA expression of Toll-like receptor-4 （TLR4） in BV-2 cells during mimic ischemia-reperfusion （I/R） injury in vitro. BV-2 cells, a mouse microglia line, were...This study investigated the effects of propofol on the mRNA expression of Toll-like receptor-4 （TLR4） in BV-2 cells during mimic ischemia-reperfusion （I/R） injury in vitro. BV-2 cells, a mouse microglia line, were cultured and divided into 4 groups at random： control group （group C）, ischemia/reperfusion group （group I/R）, low-dose propofol （25 μmol/L） intervention group （group PF25） and high-dose propofol （100 μmol/L） intervention group （group PF100）. The mRNA expression of TLR4 and NF-κB was measured by means of RT-PCR. TNF-α levels in the supernatants of BV-2 cells were detected by ELISA. The results showed that the mRNA expression of TLR4 and NF-κB was significantly higher in groups I/R, PF25 and PF100 than in group C （P〈0.01）. And the TNF-α level in the supernatants was elevated in groups I/R, PF25 and PF100 as compared with that in group C （P〈0.01）. After pre-treatment with propofol, the mRNA expressions of TLR4 and NF-κB and the TNF-α level were significantly decreased in groups PF25 and PF100 in comparison to those in group I/R （P〈0.01）. And the decrease in those indicators was more significant in group PF100 than in group PF25 （P〈0.01）. It was concluded that propofol exerted brain-protecting effects during I/R injury by suppressing the mRNA expressions of TLR4 and NF-κB and deceasing the TNF-α level.展开更多
Objective: To explore the effects of transforming growth factor β1(TGF-β1) on dendritic cells (DC). Methods: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF-β1-treated DC (TGFβ-DC). T...Objective: To explore the effects of transforming growth factor β1(TGF-β1) on dendritic cells (DC). Methods: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF-β1-treated DC (TGFβ-DC). Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneicstimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method and IL-12p70 protein was detected by ELISA. The expression of Toll-like receptor 4 (TLR4) was analyzed by semi quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and FCM. Results: Compared to immature DC (imDC) cultured by GM-CSF alone, the TGFβ-DC express lower CD80, CD86, I-A^b and CD40. The TGFβ-DC were resistant to maturation with LPS. Maturation resistance was evident from a failure to up-regulate co-stimulatory molecules (CMs), to stimulate larger T cells proliferation and to enhance secretion of IL-12p70. We also found that TGF-β1 could down-regulate TLR4 expression on TGFβ-DC. Conclusion: TGFβ-DC are resistant to maturation stimulus (LPS) and might have some correlation with the down-modulation of TLR4 expression.展开更多
Objective:To investigate whether remifentanil induced cardioprotecting effect is associated with expression of toll-like receptor 4(TLR4),nuclear factor κB(NF-κB) and serum interleukin-6(IL-6).Methods:Fifty rabbits ...Objective:To investigate whether remifentanil induced cardioprotecting effect is associated with expression of toll-like receptor 4(TLR4),nuclear factor κB(NF-κB) and serum interleukin-6(IL-6).Methods:Fifty rabbits were randomly divided into 5 groups(n=10) according to the treatment:sham operation group(group A),ischemia-reperfusion group(group B),low-dose remifentanil group(group C),mediate-dose remifentanil group(group D),and high-dose remifentanil group(group E).Myocardial TLR4 mRNA levels,NF-κB protein expression and serum levels of IL-6 were observed in 120 min after reperfusion.Results:The myocardial expressions of TLR4 mRNA,NF-κB protein and IL-6 level in sera of groups B,C,D and E were elevated compared with group A.However,remifentanil significantly reduced the levels of TLR4 mRNA,NF-κB protein expression and serum IL-6 in groups C,D and E compared with group B.There were remarkable differences between the groups(P<0.01).Conclusion:Intravenous remifentanil has protective effect against rabbit myocardial ischemia/reperfusion injury.This effect may be associated with TLR4,NF-κB expressions on myocytes and serum level of IL-6 in a dose-dependent manner.展开更多
文摘This study investigated the effects of propofol on the mRNA expression of Toll-like receptor-4 （TLR4） in BV-2 cells during mimic ischemia-reperfusion （I/R） injury in vitro. BV-2 cells, a mouse microglia line, were cultured and divided into 4 groups at random： control group （group C）, ischemia/reperfusion group （group I/R）, low-dose propofol （25 μmol/L） intervention group （group PF25） and high-dose propofol （100 μmol/L） intervention group （group PF100）. The mRNA expression of TLR4 and NF-κB was measured by means of RT-PCR. TNF-α levels in the supernatants of BV-2 cells were detected by ELISA. The results showed that the mRNA expression of TLR4 and NF-κB was significantly higher in groups I/R, PF25 and PF100 than in group C （P〈0.01）. And the TNF-α level in the supernatants was elevated in groups I/R, PF25 and PF100 as compared with that in group C （P〈0.01）. After pre-treatment with propofol, the mRNA expressions of TLR4 and NF-κB and the TNF-α level were significantly decreased in groups PF25 and PF100 in comparison to those in group I/R （P〈0.01）. And the decrease in those indicators was more significant in group PF100 than in group PF25 （P〈0.01）. It was concluded that propofol exerted brain-protecting effects during I/R injury by suppressing the mRNA expressions of TLR4 and NF-κB and deceasing the TNF-α level.
基金Project supported by the National Natural Science Foundation of China (No. 30270571)Doctor Degree Point Foundation of Ministry of Education, China (No. 20020335080)
文摘Objective: To explore the effects of transforming growth factor β1(TGF-β1) on dendritic cells (DC). Methods: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF-β1-treated DC (TGFβ-DC). Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneicstimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method and IL-12p70 protein was detected by ELISA. The expression of Toll-like receptor 4 (TLR4) was analyzed by semi quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and FCM. Results: Compared to immature DC (imDC) cultured by GM-CSF alone, the TGFβ-DC express lower CD80, CD86, I-A^b and CD40. The TGFβ-DC were resistant to maturation with LPS. Maturation resistance was evident from a failure to up-regulate co-stimulatory molecules (CMs), to stimulate larger T cells proliferation and to enhance secretion of IL-12p70. We also found that TGF-β1 could down-regulate TLR4 expression on TGFβ-DC. Conclusion: TGFβ-DC are resistant to maturation stimulus (LPS) and might have some correlation with the down-modulation of TLR4 expression.
基金Supported by Shaanxi Provincial Scientific and Technological Research Projects (2008K13-02)
文摘Objective:To investigate whether remifentanil induced cardioprotecting effect is associated with expression of toll-like receptor 4(TLR4),nuclear factor κB(NF-κB) and serum interleukin-6(IL-6).Methods:Fifty rabbits were randomly divided into 5 groups(n=10) according to the treatment:sham operation group(group A),ischemia-reperfusion group(group B),low-dose remifentanil group(group C),mediate-dose remifentanil group(group D),and high-dose remifentanil group(group E).Myocardial TLR4 mRNA levels,NF-κB protein expression and serum levels of IL-6 were observed in 120 min after reperfusion.Results:The myocardial expressions of TLR4 mRNA,NF-κB protein and IL-6 level in sera of groups B,C,D and E were elevated compared with group A.However,remifentanil significantly reduced the levels of TLR4 mRNA,NF-κB protein expression and serum IL-6 in groups C,D and E compared with group B.There were remarkable differences between the groups(P<0.01).Conclusion:Intravenous remifentanil has protective effect against rabbit myocardial ischemia/reperfusion injury.This effect may be associated with TLR4,NF-κB expressions on myocytes and serum level of IL-6 in a dose-dependent manner.
