Tomato spotted wilt virus(TSWV)is an important virus that has rapidly spread throughout the world.TSWV seriously hinders the production of tomato(Solanum lycopersicum)and other plants.In order to discover more new gen...Tomato spotted wilt virus(TSWV)is an important virus that has rapidly spread throughout the world.TSWV seriously hinders the production of tomato(Solanum lycopersicum)and other plants.In order to discover more new genes and metabolites related to TSWV resistance in tomato plants,the genes and metabolites related to the resistance of tomato plants inoculated with TSWV were identified and studied herein.The tomato TSWV-resistance line YNAU335(335)and TSWV-susceptible lines NO5 and 96172I(961)were used as the transcriptome and metabolome research materials.Transcriptomic and metabolomic techniques were used to analyze the gene and metabolite response mechanisms to TSWV inoculation.A total of 3566,2951,and 2674 differentially expressed genes(DEGs)were identified in lines 335,NO5,and961,respectively.Meanwhile,208,228,and 273 differentially accumulated metabolites(DAMs)were identified in lines 335,NO5,and 961,respectively.In line 335,the number of DEGs was the highest,but the number of DAMs was lowest.Furthermore,903 DEGs and 94 DAMs were common to the response to TSWV in the three inbred lines.The 903 DEGs and 94 DAMs were mainly enriched in the plant hormone signal transduction and flavonoid synthesis pathways.In addition,many nucleotide-binding site-leucine-rich repeat genes and transcription factors were found that might be involved in the TSWV response.These results provide new insights into TSWV resistance mechanisms.展开更多
Tomato spotted wilt(TSW)is a serious virus disease of peanut in the United States.Breeding for TSWV resistance would be facilitated by the implementation of marker-assisted selection in breeding programs;however,genes...Tomato spotted wilt(TSW)is a serious virus disease of peanut in the United States.Breeding for TSWV resistance would be facilitated by the implementation of marker-assisted selection in breeding programs;however,genes associated with resistance have not been identified.Association mapping is a type of genetic mapping that can exploit relationships between markers and traits in many lineages.The objectives of this study were to examine genetic diversity and population structure in the U.S.peanut mini-core collection using simple sequence repeat(SSR)markers,and to conduct association mapping between SSR markers and TSWV resistance in cultivated peanuts.One hundred and thirty-three SSR markers were used for genotyping 104 accessions.Four subpopulations,generally corresponding to botanical varieties,were classified by population structure analysis.Association mapping analysis indicated that five markers:pP GPseq5D5,GM1135,GM1991,TC23C08,and TC24C06,were consistently associated with TSW resistance by the Q,PCA,Q+K,and PCA+K models.These markers together explained 36.4%of the phenotypic variance.Moreover,pP GPseq5D5 and GM1991 were associated with both visual symptoms of TSWV and ELISA values with a high R^2.The potential of these markers for use in a marker-assisted selection program to breed peanut for resistance to TSWV is discussed.展开更多
Tomato spotted wilt orthotospovirus(TSWV)causes substantial economic losses to vegetables and other crops.TSWV is mainly transmitted by thrips in a persistent and proliferative manner,and its most efficient vector is ...Tomato spotted wilt orthotospovirus(TSWV)causes substantial economic losses to vegetables and other crops.TSWV is mainly transmitted by thrips in a persistent and proliferative manner,and its most efficient vector is the western flower thrips,Frankliniella occidentalis(Pergande).In moving from the thrips midgut to the salivary glands in preparation for transmission,the virions must overcome multiple barriers.Although several proteins that interact with TSWV in thrips have been characterized,we hypothesized that additional thrips proteins interact with TSWV and facilitate its transmission.In the current study,67 F occidentalis proteins that interact with GN(a structural glycoprotein)were identified using a split-ubiquitin membrane-based yeast 2-hybrid(MbY2H)system.Three proteins,apolipoprotein-D(ApoD),orai-2-like(Orai),and obstructor-E-like isoform X2(Obst),were selected for further study based on their high abundance and interaction strength;their interactions with Gn were confirmed by MbY2H,yeastβ-galactosidase and luciferase complementation assays.The relative expressions of ApoD and Orai were significantly down-regulated but that of Obst was significantly up-regulated in viruliferous thrips.When interfering with Obst in larval stage,the TSWV acquisition rate in 3 independent experiments was significantly decreased by 26%,40%,and 35%,respectively.In addition,when Obst was silenced in adults,the virus titer was significantly decreased,and the TSWV transmission rate decreased from 66.7%to 31.9%using the leaf disk method and from 86.67%to 43.33%using the living plant method.However,the TSWV acquisition and transmission rates were not affected by interference with the ApoD or Orai gene.The results indicate that Obst may play an important role in TSWV acquisition and transmission in Frankliniella occidentalis.展开更多
基金funded by the National Natural Science Foundation of China(Grant Nos.32160715,31660576,31760583)the Joint Project of Basic Agricultural Research in Yunnan Province(Grant No.2018FG001-004)+3 种基金Yunnan Luxi County Vegetable Industry Science and Technology Mission project(Grant No.202204BI090006)the General Project of Yunnan Science and Technology Plan(Grant No.2016FB064)High-level Scientific Research Foundation of Yunnan Agricultural University(Grant No.KY2022-27)Research and Integrated Applications of Key Technology in Standardized Production of Facility Vegetables(Grant No.202102AE090005)。
文摘Tomato spotted wilt virus(TSWV)is an important virus that has rapidly spread throughout the world.TSWV seriously hinders the production of tomato(Solanum lycopersicum)and other plants.In order to discover more new genes and metabolites related to TSWV resistance in tomato plants,the genes and metabolites related to the resistance of tomato plants inoculated with TSWV were identified and studied herein.The tomato TSWV-resistance line YNAU335(335)and TSWV-susceptible lines NO5 and 96172I(961)were used as the transcriptome and metabolome research materials.Transcriptomic and metabolomic techniques were used to analyze the gene and metabolite response mechanisms to TSWV inoculation.A total of 3566,2951,and 2674 differentially expressed genes(DEGs)were identified in lines 335,NO5,and961,respectively.Meanwhile,208,228,and 273 differentially accumulated metabolites(DAMs)were identified in lines 335,NO5,and 961,respectively.In line 335,the number of DEGs was the highest,but the number of DAMs was lowest.Furthermore,903 DEGs and 94 DAMs were common to the response to TSWV in the three inbred lines.The 903 DEGs and 94 DAMs were mainly enriched in the plant hormone signal transduction and flavonoid synthesis pathways.In addition,many nucleotide-binding site-leucine-rich repeat genes and transcription factors were found that might be involved in the TSWV response.These results provide new insights into TSWV resistance mechanisms.
基金the Peanut Foundation (04-811-16)the National Peanut Board (RIA16PID456BID1426-CC)+1 种基金Alabama Peanut Producers Associationthe Hatch program of the USDA-NIFA
文摘Tomato spotted wilt(TSW)is a serious virus disease of peanut in the United States.Breeding for TSWV resistance would be facilitated by the implementation of marker-assisted selection in breeding programs;however,genes associated with resistance have not been identified.Association mapping is a type of genetic mapping that can exploit relationships between markers and traits in many lineages.The objectives of this study were to examine genetic diversity and population structure in the U.S.peanut mini-core collection using simple sequence repeat(SSR)markers,and to conduct association mapping between SSR markers and TSWV resistance in cultivated peanuts.One hundred and thirty-three SSR markers were used for genotyping 104 accessions.Four subpopulations,generally corresponding to botanical varieties,were classified by population structure analysis.Association mapping analysis indicated that five markers:pP GPseq5D5,GM1135,GM1991,TC23C08,and TC24C06,were consistently associated with TSW resistance by the Q,PCA,Q+K,and PCA+K models.These markers together explained 36.4%of the phenotypic variance.Moreover,pP GPseq5D5 and GM1991 were associated with both visual symptoms of TSWV and ELISA values with a high R^2.The potential of these markers for use in a marker-assisted selection program to breed peanut for resistance to TSWV is discussed.
基金the China Agriculture Research System(CARS-24-C-02)the National Natural Science Foundation of China(32272548)+1 种基金the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)the Hainan Major Science and Technology Project(ZDKJ2021007)。
文摘Tomato spotted wilt orthotospovirus(TSWV)causes substantial economic losses to vegetables and other crops.TSWV is mainly transmitted by thrips in a persistent and proliferative manner,and its most efficient vector is the western flower thrips,Frankliniella occidentalis(Pergande).In moving from the thrips midgut to the salivary glands in preparation for transmission,the virions must overcome multiple barriers.Although several proteins that interact with TSWV in thrips have been characterized,we hypothesized that additional thrips proteins interact with TSWV and facilitate its transmission.In the current study,67 F occidentalis proteins that interact with GN(a structural glycoprotein)were identified using a split-ubiquitin membrane-based yeast 2-hybrid(MbY2H)system.Three proteins,apolipoprotein-D(ApoD),orai-2-like(Orai),and obstructor-E-like isoform X2(Obst),were selected for further study based on their high abundance and interaction strength;their interactions with Gn were confirmed by MbY2H,yeastβ-galactosidase and luciferase complementation assays.The relative expressions of ApoD and Orai were significantly down-regulated but that of Obst was significantly up-regulated in viruliferous thrips.When interfering with Obst in larval stage,the TSWV acquisition rate in 3 independent experiments was significantly decreased by 26%,40%,and 35%,respectively.In addition,when Obst was silenced in adults,the virus titer was significantly decreased,and the TSWV transmission rate decreased from 66.7%to 31.9%using the leaf disk method and from 86.67%to 43.33%using the living plant method.However,the TSWV acquisition and transmission rates were not affected by interference with the ApoD or Orai gene.The results indicate that Obst may play an important role in TSWV acquisition and transmission in Frankliniella occidentalis.
