This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene co...This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin （Tf）, alkaline phosphatase （Alp）, leucine aminopeptidase （Lap）, arylesterase （Ary-Es）, hemoglobin-β （Hb-β）, X-protein （X-p）, carbonic anhydrase （CA）, catalase （Cat）, malate dehydrogenase （MDH）, and lysine （Ly）, whereas, albumin （A1） and postalbumin （Po） loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the ＂Mongolia group＂, and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.展开更多
Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated freque...Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated frequencies of 27 alleles reached 0.95 except for PoF, TfA,Tff,Hb-βA and CAF which had reliabilities of 0.5222,0.7478,0.5222,0.6212 and 0.899, respectively. Of the 12 loci tested in Tong sheep, 11 loci were polymorphic. Reliability of the estimated frequency of 25 alleles reached 0.95 except for TfA, TfE and CAF which had reliabilities of 0.931,0.6922 and 0.7924, respectively. The average heterozygosity () and average homozygosity (J) was computed and the J of the two sheep colonies was 0.6619 and 0.6448, respectively. Consistent with our conclusions based on genetic data, previous research divided the native sheep populations of East and South Central Asia into three group: the“Mongolian group”,“South-Asian group” and“European group”. Consequently, the degree of genetic similarity between populations and known groups would seem to provide a reliable means of determining the genetic relationships between populations and may reflect the true genetic origin of Hu sheep and Tong sheep in China [Acta Zoologica Sinica 49(1):134-138,2003].展开更多
基金the International Cooperation Item of the National Natural Science Foundation of China (No. 30213009, 30310103007, 30410103150)Natural Science Foundation of Jiangsu Province of China (No. BK2007556)+1 种基金Basic Natura Science Foundation for Colleges and Universities Jiangsu Province (No. NK051039) the New Century Talent Project of Yangzhou University in China.
文摘This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin （Tf）, alkaline phosphatase （Alp）, leucine aminopeptidase （Lap）, arylesterase （Ary-Es）, hemoglobin-β （Hb-β）, X-protein （X-p）, carbonic anhydrase （CA）, catalase （Cat）, malate dehydrogenase （MDH）, and lysine （Ly）, whereas, albumin （A1） and postalbumin （Po） loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the ＂Mongolia group＂, and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.
文摘Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated frequencies of 27 alleles reached 0.95 except for PoF, TfA,Tff,Hb-βA and CAF which had reliabilities of 0.5222,0.7478,0.5222,0.6212 and 0.899, respectively. Of the 12 loci tested in Tong sheep, 11 loci were polymorphic. Reliability of the estimated frequency of 25 alleles reached 0.95 except for TfA, TfE and CAF which had reliabilities of 0.931,0.6922 and 0.7924, respectively. The average heterozygosity () and average homozygosity (J) was computed and the J of the two sheep colonies was 0.6619 and 0.6448, respectively. Consistent with our conclusions based on genetic data, previous research divided the native sheep populations of East and South Central Asia into three group: the“Mongolian group”,“South-Asian group” and“European group”. Consequently, the degree of genetic similarity between populations and known groups would seem to provide a reliable means of determining the genetic relationships between populations and may reflect the true genetic origin of Hu sheep and Tong sheep in China [Acta Zoologica Sinica 49(1):134-138,2003].
