BACKGROUND Lumbar disc herniation(LDH)commonly occurs during spinal surgery;LDH is on the increase in younger patients and is classified as"paralysis"and"back pain."Sanhanchushi Tongbi(SPST)is a cu...BACKGROUND Lumbar disc herniation(LDH)commonly occurs during spinal surgery;LDH is on the increase in younger patients and is classified as"paralysis"and"back pain."Sanhanchushi Tongbi(SPST)is a customized prescription.It disperses cold,relieves pain,removes cold from the meridians and viscera,and treats neuropathic pain.However,few studies have investigated its mechanism of pain relief.AIM To observe the clinical therapeutic effects on LDH treated with self-prescribed SPST.METHODS A total of 211 patients with LDH syndrome were divided into two groups:107 patients in the control group were treated with conventional massage combined with traction,and 104 patients in the observation group were treated with a combination of the control regimen and self-prescribed oral SPST.The patients were treated for 4 wk.Indices of traditional Chinese medicine(TCM)syndrome score and serum inflammatory factor levels were measured.RESULTS After therapy,the TCM syndrome score in the observation group was significantly lower than that in the control group(P<0.05).The main symptoms,clinical signs,daily activities,and Japanese Orthopedic Association scores in the observation group were significantly higher than those in the control group after therapy(P<0.05).The levels of tumor necrosis factor-α,interleukin-6,and C-reactive protein were lower in the observation group than in the control group(P<0.05).In the observation group,superoxide dismutase levels were significantly higher,whereas malondialdehyde levels were significantly lower,compared with the control group(P<0.05).The overall efficacy rate in the observation group was 96.15%,which was substantially higher than that in the control group(88.79%;P<0.05).CONCLUSION Self-prescribed SPST can reduce the levels of inflammatory and pain-causing factors as well as lumbar pain in patients with LDH.展开更多
Objective： To study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule （清络通痹颗粒, QTG） on osteoclast differentiation in rheumatoid arthritis in rats. Methods： Fibroblast and monocyte co-c...Objective： To study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule （清络通痹颗粒, QTG） on osteoclast differentiation in rheumatoid arthritis in rats. Methods： Fibroblast and monocyte co-culture were used to induce osteoclast differentiation in adjuvant-induced arthritic （AIA） rats. Serum containing QTG was prepared and added to the osteoclasts, and activation of the tumor necrosis factor receptorassociated factor 6/mitogen-activated protein kinase/nuclear factor of activated T cells, cytoplasmic1 （TRAF6/ MAPK/NFATcl） pathways was examined. Results： The induced osteoclasts were multinucleated and stained positive for tartrate-resistant acid phosphatase （TRAP） staining. Serum containing QTG at 14.4, 7.2 or 3.6 g/kg inhibited the activation of TRAF6, extracellular regulated protein kinase （ERK）1/2, c-Jun N-terminal kinase （JNK） and p38 and decreased the percentage of cells with nuclear NFATcl in a dose-dependent manner, the high and middle doses exhibited clear inhibitory activity （P〈0.01 and P〈0.05, respectively）. After the addition of MAPK inhibitors, the NFATcl expression showed no significant difference compared with the control group （P〉0.05）. Conclusions： Serum containing QTG could generally inhibit the TRAF6/MAPK pathways and possibly inhibit the NFATcl pathway. In addition, QTG may regulate other signaling pathways that are related to osteoclast differentiation and maturation.展开更多
目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,...目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选苍膝通痹胶囊的活性成分及对应靶点;运用Drugbank、人类基因数据库(the human gene database,GeneCards)、药物靶标数据库(therapeutic targetdatabase,TTD)、比较毒理基因组学数据库(comparative toxicogenomics database,CTD)检索OA的已知疾病靶点;使用Venny平台得到苍膝通痹胶囊治疗OA的药效靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络,运用STRING数据库建立蛋白-蛋白互作网络(protein-protein interactions,PPI),利用CytoHubba工具对所得PPI网络进行拓扑分析筛选出关键靶点。借助DAVID数据库对作用靶点进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)获取其潜在作用机制,构建苍膝通痹胶囊“通路-靶点”作用网络。从RCSBPDB数据库获取靶点的晶体结构文件,从Pubchem数据库获取活性成分的结构文件,利用Auto vina软件进行分子对接验证。结果:共得到苍膝通痹胶囊活性成分60个、作用靶点140个。OA疾病靶点469个,苍膝通痹胶囊治疗OA的靶点52个。核心成分有槲皮素、β-谷甾醇、豆甾醇、木犀草素等。核心靶点包括IL-1β、IL-6、VEGFA、MAPK、JUN等。通路主要涉及PI3K-Akt、NF-κB、FoxO、雌激素、MAPK通路等。有效成分在与核心靶点对接中具有较强的结合能力。结论:苍膝通痹胶囊通过调控细胞增殖、抑制炎症等多成分、多靶点、多通路治疗OA。展开更多
文摘BACKGROUND Lumbar disc herniation(LDH)commonly occurs during spinal surgery;LDH is on the increase in younger patients and is classified as"paralysis"and"back pain."Sanhanchushi Tongbi(SPST)is a customized prescription.It disperses cold,relieves pain,removes cold from the meridians and viscera,and treats neuropathic pain.However,few studies have investigated its mechanism of pain relief.AIM To observe the clinical therapeutic effects on LDH treated with self-prescribed SPST.METHODS A total of 211 patients with LDH syndrome were divided into two groups:107 patients in the control group were treated with conventional massage combined with traction,and 104 patients in the observation group were treated with a combination of the control regimen and self-prescribed oral SPST.The patients were treated for 4 wk.Indices of traditional Chinese medicine(TCM)syndrome score and serum inflammatory factor levels were measured.RESULTS After therapy,the TCM syndrome score in the observation group was significantly lower than that in the control group(P<0.05).The main symptoms,clinical signs,daily activities,and Japanese Orthopedic Association scores in the observation group were significantly higher than those in the control group after therapy(P<0.05).The levels of tumor necrosis factor-α,interleukin-6,and C-reactive protein were lower in the observation group than in the control group(P<0.05).In the observation group,superoxide dismutase levels were significantly higher,whereas malondialdehyde levels were significantly lower,compared with the control group(P<0.05).The overall efficacy rate in the observation group was 96.15%,which was substantially higher than that in the control group(88.79%;P<0.05).CONCLUSION Self-prescribed SPST can reduce the levels of inflammatory and pain-causing factors as well as lumbar pain in patients with LDH.
基金Supported by the National Natural Science Foundation of China(No.81072749)the Ministry of Education of Overseas Returnees Research Fund(No.2006331)+3 种基金Six Talent Peaks Subject of Jiangsu Province(No.2007)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.20116)Jiangsu Province Ordinary University Innovative Research ProjectOrdinary University Innovative Research Project of Jiangsu Province(No.2011)
文摘Objective： To study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule （清络通痹颗粒, QTG） on osteoclast differentiation in rheumatoid arthritis in rats. Methods： Fibroblast and monocyte co-culture were used to induce osteoclast differentiation in adjuvant-induced arthritic （AIA） rats. Serum containing QTG was prepared and added to the osteoclasts, and activation of the tumor necrosis factor receptorassociated factor 6/mitogen-activated protein kinase/nuclear factor of activated T cells, cytoplasmic1 （TRAF6/ MAPK/NFATcl） pathways was examined. Results： The induced osteoclasts were multinucleated and stained positive for tartrate-resistant acid phosphatase （TRAP） staining. Serum containing QTG at 14.4, 7.2 or 3.6 g/kg inhibited the activation of TRAF6, extracellular regulated protein kinase （ERK）1/2, c-Jun N-terminal kinase （JNK） and p38 and decreased the percentage of cells with nuclear NFATcl in a dose-dependent manner, the high and middle doses exhibited clear inhibitory activity （P〈0.01 and P〈0.05, respectively）. After the addition of MAPK inhibitors, the NFATcl expression showed no significant difference compared with the control group （P〉0.05）. Conclusions： Serum containing QTG could generally inhibit the TRAF6/MAPK pathways and possibly inhibit the NFATcl pathway. In addition, QTG may regulate other signaling pathways that are related to osteoclast differentiation and maturation.
文摘目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选苍膝通痹胶囊的活性成分及对应靶点;运用Drugbank、人类基因数据库(the human gene database,GeneCards)、药物靶标数据库(therapeutic targetdatabase,TTD)、比较毒理基因组学数据库(comparative toxicogenomics database,CTD)检索OA的已知疾病靶点;使用Venny平台得到苍膝通痹胶囊治疗OA的药效靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络,运用STRING数据库建立蛋白-蛋白互作网络(protein-protein interactions,PPI),利用CytoHubba工具对所得PPI网络进行拓扑分析筛选出关键靶点。借助DAVID数据库对作用靶点进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)获取其潜在作用机制,构建苍膝通痹胶囊“通路-靶点”作用网络。从RCSBPDB数据库获取靶点的晶体结构文件,从Pubchem数据库获取活性成分的结构文件,利用Auto vina软件进行分子对接验证。结果:共得到苍膝通痹胶囊活性成分60个、作用靶点140个。OA疾病靶点469个,苍膝通痹胶囊治疗OA的靶点52个。核心成分有槲皮素、β-谷甾醇、豆甾醇、木犀草素等。核心靶点包括IL-1β、IL-6、VEGFA、MAPK、JUN等。通路主要涉及PI3K-Akt、NF-κB、FoxO、雌激素、MAPK通路等。有效成分在与核心靶点对接中具有较强的结合能力。结论:苍膝通痹胶囊通过调控细胞增殖、抑制炎症等多成分、多靶点、多通路治疗OA。
