BACKGROUND Lumbar disc herniation(LDH)commonly occurs during spinal surgery;LDH is on the increase in younger patients and is classified as"paralysis"and"back pain."Sanhanchushi Tongbi(SPST)is a cu...BACKGROUND Lumbar disc herniation(LDH)commonly occurs during spinal surgery;LDH is on the increase in younger patients and is classified as"paralysis"and"back pain."Sanhanchushi Tongbi(SPST)is a customized prescription.It disperses cold,relieves pain,removes cold from the meridians and viscera,and treats neuropathic pain.However,few studies have investigated its mechanism of pain relief.AIM To observe the clinical therapeutic effects on LDH treated with self-prescribed SPST.METHODS A total of 211 patients with LDH syndrome were divided into two groups:107 patients in the control group were treated with conventional massage combined with traction,and 104 patients in the observation group were treated with a combination of the control regimen and self-prescribed oral SPST.The patients were treated for 4 wk.Indices of traditional Chinese medicine(TCM)syndrome score and serum inflammatory factor levels were measured.RESULTS After therapy,the TCM syndrome score in the observation group was significantly lower than that in the control group(P<0.05).The main symptoms,clinical signs,daily activities,and Japanese Orthopedic Association scores in the observation group were significantly higher than those in the control group after therapy(P<0.05).The levels of tumor necrosis factor-α,interleukin-6,and C-reactive protein were lower in the observation group than in the control group(P<0.05).In the observation group,superoxide dismutase levels were significantly higher,whereas malondialdehyde levels were significantly lower,compared with the control group(P<0.05).The overall efficacy rate in the observation group was 96.15%,which was substantially higher than that in the control group(88.79%;P<0.05).CONCLUSION Self-prescribed SPST can reduce the levels of inflammatory and pain-causing factors as well as lumbar pain in patients with LDH.展开更多
目的研究通痹颗粒对胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠铁调素(hepcidin,Hepc)、Janus激酶(janus kinase,JAK)2/信号转导子和转录激活子(signal transduction and activator of transcription,STAT)3信号通路的影响...目的研究通痹颗粒对胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠铁调素(hepcidin,Hepc)、Janus激酶(janus kinase,JAK)2/信号转导子和转录激活子(signal transduction and activator of transcription,STAT)3信号通路的影响。方法选取36只雌性SD大鼠随机分成空白组、模型组、阳性对照组和通痹颗粒低、中、高剂量组,每组6只。空白组不予处理,其余组用牛Ⅱ型胶原建立CIA模型。造模完成后,空白组、模型组予生理盐水灌胃,其余各组分别以巴瑞替尼片和低、中、高剂量通痹颗粒灌胃。每天1次,连续4周。HE染色行滑膜组织病理学观察;酶联免疫吸附法测定血清Hepc、白细胞介素6(interleukin 6,IL-6)水平;逆转录-聚合酶链反应法测定滑膜中JAK2、STAT3、细胞信号因子传导抑制体(suppressor of cytokine signaling,SOCS)1、SOCS3的mRNA相对表达量;Western blot法检测滑膜中JAK2、p-JAK2、STAT3、p-STAT3、SOCS1、SOCS3的蛋白表达量。结果模型组见滑膜上皮结构缺损,滑膜重度增生,排列紊乱,并有大量炎症细胞浸润和多个血管翳形成;各给药组滑膜炎症均有所减轻,阳性对照组优于通痹颗粒高剂量组,通痹颗粒中、高剂量组优于低剂量组。与模型组相比,各给药组关节炎指数评分、血清Hepc和IL-6水平均显著降低(P<0.01);与阳性对照组相比,通痹颗粒中、低剂量组关节炎指数评分、血清Hepc和IL-6水平均升高(P<0.05)。与模型组比较,阳性对照组和通痹颗粒低、中、高剂量组JAK2、STAT3 mRNA和蛋白以及p-JAK2、p-STAT3的蛋白表达量均降低(P<0.05),而通路抑制因子SOCS1、SOCS3 mRNA和蛋白的表达均升高(P<0.05);与阳性对照组比较,通痹颗粒各剂量组JAK2、STAT3 mRNA和蛋白以及p-JAK2、p-STAT3的蛋白表达量均升高(P<0.05),而SOCS1、SOCS3 mRNA和蛋白的表达均降低(P<0.05)。结论通痹颗粒能够改善CIA大鼠滑膜炎症,其机制可能与抑制JAK2/STAT3信号通路而减少Hepc的表达有关。展开更多
目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,...目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选苍膝通痹胶囊的活性成分及对应靶点;运用Drugbank、人类基因数据库(the human gene database,GeneCards)、药物靶标数据库(therapeutic targetdatabase,TTD)、比较毒理基因组学数据库(comparative toxicogenomics database,CTD)检索OA的已知疾病靶点;使用Venny平台得到苍膝通痹胶囊治疗OA的药效靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络,运用STRING数据库建立蛋白-蛋白互作网络(protein-protein interactions,PPI),利用CytoHubba工具对所得PPI网络进行拓扑分析筛选出关键靶点。借助DAVID数据库对作用靶点进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)获取其潜在作用机制,构建苍膝通痹胶囊“通路-靶点”作用网络。从RCSBPDB数据库获取靶点的晶体结构文件,从Pubchem数据库获取活性成分的结构文件,利用Auto vina软件进行分子对接验证。结果:共得到苍膝通痹胶囊活性成分60个、作用靶点140个。OA疾病靶点469个,苍膝通痹胶囊治疗OA的靶点52个。核心成分有槲皮素、β-谷甾醇、豆甾醇、木犀草素等。核心靶点包括IL-1β、IL-6、VEGFA、MAPK、JUN等。通路主要涉及PI3K-Akt、NF-κB、FoxO、雌激素、MAPK通路等。有效成分在与核心靶点对接中具有较强的结合能力。结论:苍膝通痹胶囊通过调控细胞增殖、抑制炎症等多成分、多靶点、多通路治疗OA。展开更多
文摘BACKGROUND Lumbar disc herniation(LDH)commonly occurs during spinal surgery;LDH is on the increase in younger patients and is classified as"paralysis"and"back pain."Sanhanchushi Tongbi(SPST)is a customized prescription.It disperses cold,relieves pain,removes cold from the meridians and viscera,and treats neuropathic pain.However,few studies have investigated its mechanism of pain relief.AIM To observe the clinical therapeutic effects on LDH treated with self-prescribed SPST.METHODS A total of 211 patients with LDH syndrome were divided into two groups:107 patients in the control group were treated with conventional massage combined with traction,and 104 patients in the observation group were treated with a combination of the control regimen and self-prescribed oral SPST.The patients were treated for 4 wk.Indices of traditional Chinese medicine(TCM)syndrome score and serum inflammatory factor levels were measured.RESULTS After therapy,the TCM syndrome score in the observation group was significantly lower than that in the control group(P<0.05).The main symptoms,clinical signs,daily activities,and Japanese Orthopedic Association scores in the observation group were significantly higher than those in the control group after therapy(P<0.05).The levels of tumor necrosis factor-α,interleukin-6,and C-reactive protein were lower in the observation group than in the control group(P<0.05).In the observation group,superoxide dismutase levels were significantly higher,whereas malondialdehyde levels were significantly lower,compared with the control group(P<0.05).The overall efficacy rate in the observation group was 96.15%,which was substantially higher than that in the control group(88.79%;P<0.05).CONCLUSION Self-prescribed SPST can reduce the levels of inflammatory and pain-causing factors as well as lumbar pain in patients with LDH.
文摘目的:采用网络药理学方法及分子对接技术探讨苍膝通痹胶囊治疗骨关节炎(osteoarthritis,OA)的分子机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)筛选苍膝通痹胶囊的活性成分及对应靶点;运用Drugbank、人类基因数据库(the human gene database,GeneCards)、药物靶标数据库(therapeutic targetdatabase,TTD)、比较毒理基因组学数据库(comparative toxicogenomics database,CTD)检索OA的已知疾病靶点;使用Venny平台得到苍膝通痹胶囊治疗OA的药效靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络,运用STRING数据库建立蛋白-蛋白互作网络(protein-protein interactions,PPI),利用CytoHubba工具对所得PPI网络进行拓扑分析筛选出关键靶点。借助DAVID数据库对作用靶点进行基因本体论功能富集分析(gene ontology,GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)获取其潜在作用机制,构建苍膝通痹胶囊“通路-靶点”作用网络。从RCSBPDB数据库获取靶点的晶体结构文件,从Pubchem数据库获取活性成分的结构文件,利用Auto vina软件进行分子对接验证。结果:共得到苍膝通痹胶囊活性成分60个、作用靶点140个。OA疾病靶点469个,苍膝通痹胶囊治疗OA的靶点52个。核心成分有槲皮素、β-谷甾醇、豆甾醇、木犀草素等。核心靶点包括IL-1β、IL-6、VEGFA、MAPK、JUN等。通路主要涉及PI3K-Akt、NF-κB、FoxO、雌激素、MAPK通路等。有效成分在与核心靶点对接中具有较强的结合能力。结论:苍膝通痹胶囊通过调控细胞增殖、抑制炎症等多成分、多靶点、多通路治疗OA。
