[Objectives]The paper was to investigate and assess the species,distribution and hazard status of invasive alien plants on the campus of Tongren University.[Methods]A survey was conducted using line survey method,and ...[Objectives]The paper was to investigate and assess the species,distribution and hazard status of invasive alien plants on the campus of Tongren University.[Methods]A survey was conducted using line survey method,and the risk assessment index system of invasive alien plants was established by analytic hierarchy process(AHP).[Results]There were 11 species of invasive alien plants on the campus of Tongren University,belonging to 8 genera and 5 families.There were 6 species of invasive alien plants belonging to Asteraceae,accounting for 54.55%of the total.In terms of the origin of invasive plants,most of them were native to North America and Europe,accounting for 27.27%of the total.In terms of harmful level,there were no high-risk invasive plants on the campus of Tongren University,but Alternanthera philoxeroides had the highest score and was the most harmful.[Conclusions]The present situation of alien plant invasion on the campus of Tongren University is closely related to the ecological environment of the campus.It is suggested to strengthen the management of campus plants,focus on the prevention and control of A.philoxeroides,and carry out regular investigation of invasive alien plants on campus to ensure the ecological safety of the campus.展开更多
Objective The Cenozoic Indo-Asian collision caused significant crustal shortening and plateau uplift in the central Tibet. The extrusion tectonic model has been widely accepted to explain the strike-slip faults around...Objective The Cenozoic Indo-Asian collision caused significant crustal shortening and plateau uplift in the central Tibet. The extrusion tectonic model has been widely accepted to explain the strike-slip faults around the Tibetan Plateau. Previous studies indicate that the lower crust flow is the main drive force of the extrusion tectonics. Whether mantle extrusion process occurred during the Cenozoic uplift is a major problem to be addressed, which is significant for understanding the uplift mechanism and tectonic evolution of the Tibetan Plateau.展开更多
Objective:To evaluate the clinical effect of Tongren Dahuoluo Pill(同仁大活络丸)on knee osteoarthritis by musculoskeletal ultrasonography.Methods:From March 2019 to December 2019,45 patients with knee osteoarthritis w...Objective:To evaluate the clinical effect of Tongren Dahuoluo Pill(同仁大活络丸)on knee osteoarthritis by musculoskeletal ultrasonography.Methods:From March 2019 to December 2019,45 patients with knee osteoarthritis were collected from the outpatient department of the First Medical Center of PLA General Hospital.Tongren Dahuoluo Pill(同仁大活络丸)was given oral treatment for 12 weeks.VAS score,Lequesne score and WOMAC score were recorded before and after treatment,and the changes of knee joint effusion and synovial hyperplasia were observed by musculoskeletal ultrasonography.Results:Among the 45 patients,18 were male and 27 were female,with the average age of(62.07±9.08)years old,and average disease course of(6.83±7.01)years.The VAS score,Lequesne score and WOMAC score were significantly reduced compared with before treatment,and the difference was statistically significant(P<0.01).We observed that joint effusion range and joint synovial hyperplasia thickness of knee osteoarthritis significantly reduced with Tongren Dahuoluo Pill(同仁大活络丸)treatment by musculoskeletal ultrasonography,and the difference were statistically significant(P<0.01).Conclusion:Tongren Dahuoluo Pill(同仁大活络丸)can not only relieve the clinical symptoms of knee osteoarthritis,but also effectively inhibit synovitis hyperplasia of knee joint.展开更多
Tongren Dahuoluo Wan has been a popular traditional Chinese medicine in international pharmaceutical markets for hundreds of years. Leopard bone powder is the key element in its formulation. However, the leopard has b...Tongren Dahuoluo Wan has been a popular traditional Chinese medicine in international pharmaceutical markets for hundreds of years. Leopard bone powder is the key element in its formulation. However, the leopard has been listed for wildlife conservation, which limits the use of the leopard bone supplies. Therefore, an alternative formulation which substitutes leopard bone with zokor bone in the formula of Tongren Dahuoluo Wan is now manufactured. To develop a simple and reliable molecular method for authenticating the two patent medicines,mitochondrial nucleotide polymorphic sites of 12 S rRNA,COI and Cytb genes were screened in leopard and zokor bones, and nine pairs of species-specific primers were verified for discriminating the two species. For the patent medicine authentication, we set up a molecular diagnostic assay to resolve the difficulties of low concentration of target DNAs and presence of PCR-inhibitory substances in this complex medicine, and successfully confirmed leopard or zokor content using the nine pairs of species-specific primers. We recommend a common technical strategy for authentication of species origins in traditional Chinese medicine, and discuss the experimental solutions for technical problems of molecular diagnostic assays.展开更多
基金Supported by National Natural Science Foundation of China(31900271,32160086)Key Project of Guizhou Basic Research Program(QKHJC[2019]1455)Central Government Supporting Local Science and Technology Development Fund Project(QKZYD[2021]4010).
文摘[Objectives]The paper was to investigate and assess the species,distribution and hazard status of invasive alien plants on the campus of Tongren University.[Methods]A survey was conducted using line survey method,and the risk assessment index system of invasive alien plants was established by analytic hierarchy process(AHP).[Results]There were 11 species of invasive alien plants on the campus of Tongren University,belonging to 8 genera and 5 families.There were 6 species of invasive alien plants belonging to Asteraceae,accounting for 54.55%of the total.In terms of the origin of invasive plants,most of them were native to North America and Europe,accounting for 27.27%of the total.In terms of harmful level,there were no high-risk invasive plants on the campus of Tongren University,but Alternanthera philoxeroides had the highest score and was the most harmful.[Conclusions]The present situation of alien plant invasion on the campus of Tongren University is closely related to the ecological environment of the campus.It is suggested to strengthen the management of campus plants,focus on the prevention and control of A.philoxeroides,and carry out regular investigation of invasive alien plants on campus to ensure the ecological safety of the campus.
基金supported by the National Natural Science Foundation of China (grant No.41072052)
文摘Objective The Cenozoic Indo-Asian collision caused significant crustal shortening and plateau uplift in the central Tibet. The extrusion tectonic model has been widely accepted to explain the strike-slip faults around the Tibetan Plateau. Previous studies indicate that the lower crust flow is the main drive force of the extrusion tectonics. Whether mantle extrusion process occurred during the Cenozoic uplift is a major problem to be addressed, which is significant for understanding the uplift mechanism and tectonic evolution of the Tibetan Plateau.
文摘Objective:To evaluate the clinical effect of Tongren Dahuoluo Pill(同仁大活络丸)on knee osteoarthritis by musculoskeletal ultrasonography.Methods:From March 2019 to December 2019,45 patients with knee osteoarthritis were collected from the outpatient department of the First Medical Center of PLA General Hospital.Tongren Dahuoluo Pill(同仁大活络丸)was given oral treatment for 12 weeks.VAS score,Lequesne score and WOMAC score were recorded before and after treatment,and the changes of knee joint effusion and synovial hyperplasia were observed by musculoskeletal ultrasonography.Results:Among the 45 patients,18 were male and 27 were female,with the average age of(62.07±9.08)years old,and average disease course of(6.83±7.01)years.The VAS score,Lequesne score and WOMAC score were significantly reduced compared with before treatment,and the difference was statistically significant(P<0.01).We observed that joint effusion range and joint synovial hyperplasia thickness of knee osteoarthritis significantly reduced with Tongren Dahuoluo Pill(同仁大活络丸)treatment by musculoskeletal ultrasonography,and the difference were statistically significant(P<0.01).Conclusion:Tongren Dahuoluo Pill(同仁大活络丸)can not only relieve the clinical symptoms of knee osteoarthritis,but also effectively inhibit synovitis hyperplasia of knee joint.
基金supported by projects of Beijing Municipal Science & Technology Commission (D08080203640903)National Natural Science Foundation of China (31672379)
文摘Tongren Dahuoluo Wan has been a popular traditional Chinese medicine in international pharmaceutical markets for hundreds of years. Leopard bone powder is the key element in its formulation. However, the leopard has been listed for wildlife conservation, which limits the use of the leopard bone supplies. Therefore, an alternative formulation which substitutes leopard bone with zokor bone in the formula of Tongren Dahuoluo Wan is now manufactured. To develop a simple and reliable molecular method for authenticating the two patent medicines,mitochondrial nucleotide polymorphic sites of 12 S rRNA,COI and Cytb genes were screened in leopard and zokor bones, and nine pairs of species-specific primers were verified for discriminating the two species. For the patent medicine authentication, we set up a molecular diagnostic assay to resolve the difficulties of low concentration of target DNAs and presence of PCR-inhibitory substances in this complex medicine, and successfully confirmed leopard or zokor content using the nine pairs of species-specific primers. We recommend a common technical strategy for authentication of species origins in traditional Chinese medicine, and discuss the experimental solutions for technical problems of molecular diagnostic assays.
