Objective:To predict the mechanism of Tongxie Yaofang in the treatment of colorectal cancer(CRC).Methods:The active compounds and targets of Tongxie Yaofang were screened by the TCMSP database,and the MalaCards databa...Objective:To predict the mechanism of Tongxie Yaofang in the treatment of colorectal cancer(CRC).Methods:The active compounds and targets of Tongxie Yaofang were screened by the TCMSP database,and the MalaCards database used“Colorectal cancer”as the key word to search for CRC targets.After the two database were mapped,the overlapping targets are merged.In David 6.8 database,the GO function and KEGG pathway of intersection targets were analyzed.Cytoscape 3.7.2 was used to construct the networks of“Active compounds-Targets”and“Herbs-Active compounds-CRC-Targets”,and the core compounds and the key targets of Tongxie Yaofang in treating CRC were selected through Network Analysis.THE HUMAN PROTEIN ATLAS database was used to verify the protein expression of key genes.Using AutoDock Vina and Python scripts,the core compounds are interfaced with the key target protein receptors.Results:TCMSP database indicated that there were 34 active compounds and 114 targets of Tongxie Yaofang,while MalaCards database indicated that there were 838 targets closely related to CRC,and 52 cross targets were obtained from the two database.Go enrichment analysis showed that 1410 items were involved in the cross targets(P<0.05),and the biological process(BP)was mainly concentrated in Lipid metabolism,hormone reaction,proliferation and apoptosis of cancer cells;Cell composition(CC)mainly affected the mitochondrial membrane potential and endoplasmic reticulum stress of CRC cells;while Molecular Function(MF)mainly involves oxidoreductase activity,protein kinase activity,etc.KEGG pathway enrichment analysis indicated that 104 signaling pathways were involved in the cross targets(P<0.05)including PI3K/Akt,TNF,TLR and so on.THE HUMAN PROTEIN ATLAS database showed that PTGS2,PTGS1,and ESR1 showed stronger positive protein expression in CRC tissues than normal intestinal tissues.The combination of Wogonin,Kaempferol and beta-sitosterol with PTGS2,PTGS1 and ESR1 were lower than≤−5.0 kJ/mol that showing good affinity.Conclusion:The Tongxie Yaofang in treating CRC involves many signal pathways and biological processes,and the main active components including Wogonin,Kaempferol,beta-sitosterol,and the key targets PTGS2,PTGS1,ESR1,may be one of the important mechanisms.展开更多
Objective:To study the clinical effect of Tongxie Yaofang(痛泻要方,TXYF) Granule in treating diarrhea-predominate irritable bowel syndrome(D-IBS) and its possible mechanism.Methods:A total of 120 patients were assigne...Objective:To study the clinical effect of Tongxie Yaofang(痛泻要方,TXYF) Granule in treating diarrhea-predominate irritable bowel syndrome(D-IBS) and its possible mechanism.Methods:A total of 120 patients were assigned to two groups using stratified block randomization,80 in the intervention group and 40 in the control group.To the intervention group the TXYF granule was given at one package each time,twice a day; the control group was treated with Miyarisan three times a day,two tablets each time.The course of...展开更多
腹泻型肠易激综合征(irritable bowel syndrome with diarrhoea,IBS-D)是最常见的消化系统疾病之一,发病机制复杂,临床表现个体差异性大。目前西药主要是对症治疗,但仍存在停药后反复发作等弊端。中药治疗IBS-D辨证施治,疗效显著。IBS-...腹泻型肠易激综合征(irritable bowel syndrome with diarrhoea,IBS-D)是最常见的消化系统疾病之一,发病机制复杂,临床表现个体差异性大。目前西药主要是对症治疗,但仍存在停药后反复发作等弊端。中药治疗IBS-D辨证施治,疗效显著。IBS-D属于中医学“肠郁”“腹痛”“泄泻”等范畴,病变部位主要在肝脾。中医药疏肝健脾方法治疗IBS-D从改善脾胃功能、调节情志出发,对肝郁脾虚型IBS-D腹痛腹泻等临床症状疗效确切,其中以痛泻要方为代表。痛泻要方能够缓解肠肌痉挛导致的腹痛,改善腹泻的症状;且具有降低IBS-D模型大鼠的内脏高敏感、改善免疫功能、调节脑肠肽等作用。近几年的研究表明痛泻要方对改善肠道菌群失调、降低肠道通透性以及表观遗传等方面亦有影响。该文章全面综述了目前关于痛泻要方治疗IBS-D作用机制,为经方治疗IBS-D提供最新的理论依据。展开更多
基金Jiangsu Provincial Health Commission Guidance Project(No.Z2017012)Jiangsu Province key talent project of Youth Medicine(No.QNRC2016239)。
文摘Objective:To predict the mechanism of Tongxie Yaofang in the treatment of colorectal cancer(CRC).Methods:The active compounds and targets of Tongxie Yaofang were screened by the TCMSP database,and the MalaCards database used“Colorectal cancer”as the key word to search for CRC targets.After the two database were mapped,the overlapping targets are merged.In David 6.8 database,the GO function and KEGG pathway of intersection targets were analyzed.Cytoscape 3.7.2 was used to construct the networks of“Active compounds-Targets”and“Herbs-Active compounds-CRC-Targets”,and the core compounds and the key targets of Tongxie Yaofang in treating CRC were selected through Network Analysis.THE HUMAN PROTEIN ATLAS database was used to verify the protein expression of key genes.Using AutoDock Vina and Python scripts,the core compounds are interfaced with the key target protein receptors.Results:TCMSP database indicated that there were 34 active compounds and 114 targets of Tongxie Yaofang,while MalaCards database indicated that there were 838 targets closely related to CRC,and 52 cross targets were obtained from the two database.Go enrichment analysis showed that 1410 items were involved in the cross targets(P<0.05),and the biological process(BP)was mainly concentrated in Lipid metabolism,hormone reaction,proliferation and apoptosis of cancer cells;Cell composition(CC)mainly affected the mitochondrial membrane potential and endoplasmic reticulum stress of CRC cells;while Molecular Function(MF)mainly involves oxidoreductase activity,protein kinase activity,etc.KEGG pathway enrichment analysis indicated that 104 signaling pathways were involved in the cross targets(P<0.05)including PI3K/Akt,TNF,TLR and so on.THE HUMAN PROTEIN ATLAS database showed that PTGS2,PTGS1,and ESR1 showed stronger positive protein expression in CRC tissues than normal intestinal tissues.The combination of Wogonin,Kaempferol and beta-sitosterol with PTGS2,PTGS1 and ESR1 were lower than≤−5.0 kJ/mol that showing good affinity.Conclusion:The Tongxie Yaofang in treating CRC involves many signal pathways and biological processes,and the main active components including Wogonin,Kaempferol,beta-sitosterol,and the key targets PTGS2,PTGS1,ESR1,may be one of the important mechanisms.
基金Supported by the Fund of Hangzhou Municipal Science and Technology Administration(No.20070433Q24)
文摘Objective:To study the clinical effect of Tongxie Yaofang(痛泻要方,TXYF) Granule in treating diarrhea-predominate irritable bowel syndrome(D-IBS) and its possible mechanism.Methods:A total of 120 patients were assigned to two groups using stratified block randomization,80 in the intervention group and 40 in the control group.To the intervention group the TXYF granule was given at one package each time,twice a day; the control group was treated with Miyarisan three times a day,two tablets each time.The course of...
文摘腹泻型肠易激综合征(irritable bowel syndrome with diarrhoea,IBS-D)是最常见的消化系统疾病之一,发病机制复杂,临床表现个体差异性大。目前西药主要是对症治疗,但仍存在停药后反复发作等弊端。中药治疗IBS-D辨证施治,疗效显著。IBS-D属于中医学“肠郁”“腹痛”“泄泻”等范畴,病变部位主要在肝脾。中医药疏肝健脾方法治疗IBS-D从改善脾胃功能、调节情志出发,对肝郁脾虚型IBS-D腹痛腹泻等临床症状疗效确切,其中以痛泻要方为代表。痛泻要方能够缓解肠肌痉挛导致的腹痛,改善腹泻的症状;且具有降低IBS-D模型大鼠的内脏高敏感、改善免疫功能、调节脑肠肽等作用。近几年的研究表明痛泻要方对改善肠道菌群失调、降低肠道通透性以及表观遗传等方面亦有影响。该文章全面综述了目前关于痛泻要方治疗IBS-D作用机制,为经方治疗IBS-D提供最新的理论依据。
