Experimental Study on Effect of Tonifying Kidney Herbs on Pituitary, Ovary, and Adrenal Gland in Androgen Sterilized Rats

Objective: To observe the effect of tonifying Kidney herbs (TKH) on pituitary gland, ovary, and adrenal gland of androgen sterilized rats (ASR).Methods: ASR model was established by injecting testosterone propionate subcutaneously to Sprague-Dawley (SD) female rats of 9 days age. Morphological and endocrinological change of pituitary gland, ovary and adrenal gland in rats of 100–107 days old before and after feeding TKH extract were observed by light and electron microscope, cell cultures, immunohistochemical studies, and radioimmunoassay.Results: In the ASR groups, there were intracytoplasmic lipid drops, crinophagy, vacuole, and autophagy in pituitary gland, anovulation in ovary, and hyperplasia of reticular zone in adrenal gland. At the same time, serum follicular stimulating hormone and luteinizing hormone decreased (P < 0.05-0.005) and serum testosterone and dehydroepiandrosterone levels increased significantly (P < 0.01-0.001). These morphologiacl and endocrinological changes were all returned to normal range after the TKH treatment.

浅谈中成药再配伍治疗的可行性及意义

目的:探讨临床上应用中成药再配伍治疗疾病的方法的可行性,及在临床应用中的推广对中医药发展的意义。方法:以中医理论基础为指导,在诊断明确的前提下,应用中成药再配伍的方法对临床常见病多发病进行探索性治疗。结果:中成药再配伍在临床上对一些常见病多发病的治疗效果较为满意。结论:中成药再配伍在临床上有广阔的发展空间,且有很大的社会效益。

补肾解毒方治疗无症状HBeAg阳性慢性HBV携带者34例

目的:观察补肾解毒方治疗肾虚型乙型肝炎病毒e抗原(hepatitis Be antigen,HBe Ag)阳性慢性乙型肝炎病毒(hepatitis B virus,HBV)携带者的临床疗效。方法:将68例肾虚型乙型肝炎病毒e抗原(HBeAg)阳性的慢性HBV携带者,采用随机、双盲、安慰剂对照的临床研究方法,随机分为观察组和对照组,每组34例。观察组予补肾解毒方治疗,口服,1剂/d;对照组使用安慰剂治疗,疗程60周。观察其抗HBV-DNA疗效及对血清乙型肝炎病毒表面抗原(hepatitis B surface antigen,HBsAg)、HBeAg定量的影响。结果:60周后观察组HBe Ag转阴率、HBV-DNA阴转率均优于对照组,差异有统计学意义(P<0.05)。补肾解毒方治疗期间,未见明显不良反应发生。HBV-DNA和HBsAg定量观察组治疗前后比较,差异有统计学意义(P<0.05),治疗后2组比较差异有统计学意义(P<0.05)。结论:补肾解毒方治疗慢性HBV携带者的抗病毒疗效确切。

New Insights into the Molecular Basis of Kidney Governing Bone Theory

Kidney governing bone theory plays an important role in treating bone metabolic disease such as osteoporosis, and many tonifying kidney prescriptions/herbs are widely used in Traditional Chinese Medicine(TCM). However, the exact biological basis of kidney governing bone theory in the context of new advances in biology is still not fully established. In this paper, the content of kidney governing bone theory in biology has been fully demonstrated from different aspects. We first propose that bone and kidney mutually affect each other in pathology and physiology, particularly through homeostasis of calcium, phosphorus and fibroblast growth factor-23(FGF-23). Next, we identify that tonifying kidney prescriptions/herbs exert bone protective effects, thus treating osteoporosis by regulating bone formation and bone resorption.Furthermore, the exact molecular mechanisms of tonifying kidney prescriptions, herbs and their effective components in treating osteoporosis have been systematically reviewed. Finally, we come into the conclusion that kidney regulating bone mineral homeostasis, bone protective effects of tonifying kidney herbs and regulatory effects on bone homeostasis are all the manifestations of kidney governing bone theory.Therefore, the new insights into kidney governing bone theory in biology will promote the development of clinical practices, and drugs discovery in treating osteoporosis.

基于线粒体功能揭示《黄帝内经》“七七”理论内涵及高龄IVF-ET患者的中药干预机制

目的:研究不同年龄阶段女性卵巢颗粒细胞线粒体功能的变化及补肾中药二至天癸方对高龄妇女体外受精-胚胎移植技术(IVF-ET)结局的影响,从而验证《黄帝内经》“七七”理论的科学内涵。方法:收集山东中医药大学附属医院中西医结合生殖与遗传中心行辅助生殖技术的不孕症患者150例,按照《黄帝内经》“七七”理论分为三七、四七组(30例),五七组(60例)和六七组(60例),五七组和六七组采用随机数字+信封法分为对照组和中药组各30例,中药组自促排卵周期启动日给予二至天癸方至扳机日。观察各组之间的IVF结局,使用蛋白免疫印迹法(Western blot)测定各组卵巢颗粒细胞中线粒体融合蛋白1(Mfn1)、线粒体融合蛋白2(Mfn2),线粒体分裂蛋白1(Drp1)的含量。结果:与三七、四七组比较,五七对照组和六七对照组患者获卵数、双原核(2PN)数、可用胚胎数、优质胚胎数、临床妊娠率和活产率均明显下降(P<0.05),六七对照组鲜胚移植率明显下降(P<0.05)。与五七对照组比较,六七对照组患者患者获卵数、2PN数、可用胚胎数、优质胚胎数和临床妊娠率均明显下降(P<0.05)。与五七对照组比较,五七中药组患者获卵数、2PN数、可用胚胎数明显升高(P<0.05);与六七对照组比较,六七中药组患者获卵数明显升高(P<0.05)。与三七、四七组比较,五七和六七对照组患者卵巢颗粒细胞中Mfn1和Mfn2蛋白表达明显降低,Drp1蛋白表达升高(P<0.05);与五七对照组比较,六七对照组患者卵巢颗粒细胞中Mfn1和Mfn2蛋白表达明显降低,Drp1蛋白表达升高(P<0.05)。与五七对照组比较,五七中药组患者卵巢颗粒细胞中Mfn2蛋白表达升高,Drp1蛋白表达降低(P<0.05);与六七对照组比较,六七中药组患者卵巢颗粒细胞中Mfn1和Mfn2蛋白表达升高,Drp1蛋白表达降低(P<0.05)。结论:随着年龄的增长,女性在“五七”后IVF结局预后变差,卵巢颗粒细胞中线粒体融合能力下降,而裂变增多,从线粒体功能验证了《黄帝内经》“七七”理论的内涵。二至天癸方可以调节高龄女性的卵巢颗粒细胞线粒体功能,提高Mfn1、Mfn2表达水平,促进线粒体融合,抑制Drp1而减少线粒体分裂,缓解年龄带来的卵巢功能减退,提高卵母细胞发育潜能,改善高龄女性的IVF结局,但对于“六七”之年的超高龄女性临床效果欠佳。

Shen-Jing as a Chinese Medicine Concept Might Be a Counterpart of Stem Cells in Regenerative Medicine

As the epitome of the modern regenerative medicine, stem cells were proposed in the basic sense no more than 200 years ago. However, the concept of "stem cells" existed long before the modern medical description. The hypothesis that all things, including our sentient body, were generated from a small origin was shared between Western and Chinese people. The ancient Chinese philosophers considered Jing(also known as essence) as the origin of life. In Chinese medicine(CM), Jing is mainly stored in Kidney(Shen) and the so-called Shen-Jing(Kidney essence). Here, we propose that Shen-Jing is the CM term used to express the meaning of "origin and regeneration". This theoretical discovery has at least two applications. First, the actions underlying causing Shen-Jing deficiency, such as excess sexual intercourse, chronic diseases, and aging, might damage the function of stem cells. Second, a large number of Chinese herbs with Shen-Jing-nourishing efficacy had been proven to affect stem cell proliferation and differentiation. Therefore, if Shen-Jing in CM is equivalent with stem cells in regenerative medicine, higher effective modulators for regulating stem-cell behaviors from Kidney-tonifying herbs would be expected.