Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary traj...Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary trajectory is poorly understood. Here, by reanalysing the T.sinensis genome, we found evidence of a tetraploidization event(T. sinensis special tetraploidization, TST) that occurred approximately 15-17 million years ago(MYA) after the core eudicot-common hexaploidization(ECH or gamma) event. We characterized the synonymous nucleotide substitution rates(Ks values) of collinear genes and found that T. sinensis genes affected by the TST evolve at a slower rate than Acer yangbiense genes. Furthermore, we identified homologous genes related to polyploidization and speciation and constructed multiple alignments with different reference genomes. Notably, the significant balance of gene retention and loss characterized in the two TST-derived subgenomes suggests an autopolyploid nature of the TST. Moreover, we deduced the chromosomal karyotypes of the two subgenomes and identified 7chromosomal fusions that have shaped the T. sinensis genome;more information is available on a newly constructed karyotype platform(http://www.cgrpoee.top/Toona_sinensis/index.html). The T. sinensis genome preserves the ancestral chromosome structure of dicotyledons well and could serve as a good reference for understanding genomic changes in other Meliaceae and Sapindales plants. In addition, we verified that tandem duplication and the ECH have promoted the expansion of terpene synthase(TPS) genes;conversely, the TST seems to have inhibited expansion of these genes. This present effort has clarified the polyploidy events of the T. sinensis genome, filled gaps in the history of karyotype evolution, and laid a solid foundation for further genomic studies in the Meliaceae research community and beyond.展开更多
Three new limonoid-type triterpenoids,namely toonasins A–C(1–3)with a rare lactam E ring,along with six known compounds(4–9)were isolated from the barks of Toona sinensis.The structures of new compounds were elucid...Three new limonoid-type triterpenoids,namely toonasins A–C(1–3)with a rare lactam E ring,along with six known compounds(4–9)were isolated from the barks of Toona sinensis.The structures of new compounds were elucidated by interpretation of spectroscopic data,and the relative configuration of compound 1 was further characterized by X-ray crystallographic analyses.The isolated compounds were evaluated for their cytotoxic activities against five human tumor cell lines(HL-60,SMMC-7721,A-549,MCF-7 and SW480),and compounds 3 and 5 showed weak cytotoxicities.展开更多
In order to prolong the supply period and increase the economic value of Toona sinensis,the protected cultivation of T. sinensis was carried out on mountainous slope to achieve stagger-season planting and the year rou...In order to prolong the supply period and increase the economic value of Toona sinensis,the protected cultivation of T. sinensis was carried out on mountainous slope to achieve stagger-season planting and the year round supply. The results showed that in late October to midNovember,the temperature was moderate( 1-10℃) and suitable for the lifting of seedlings and temporary planting dormancy. The line spacing of rooted seedlings in greenhouse was( 22-24) cm ×( 4-5) cm and planting density was 900 000-1 200 000 seedlings/ha. The line spacing of seedlings was( 14-13) cm ×( 3-4) cm and planting density was 1 950 000-2 100 000 seedlings/ha. T. sinensis began to sprout at 8-10℃ and stretch at 10-12℃. When the temperature was stable at 14℃ or above,T. sinensis started bolting and emerging leaves. The terminal buds sprouted in 40-50 d for the T. sinensis planted in greenhouse with the day temperature of 15-22℃ and night temperature over 10℃; the terminal buds sprouted in about 15 d for those planted in the greenhouse with day temperature of 20-25℃ and night temperature over 10℃. T. sinensis could be picked 3 times when planted in greenhouse,and the yield was about 24 720 kg/ha.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32170236 and 31501333)the Natural Science Foundation of Hebei Province(Grant No.C2020209064)+2 种基金the Project of Youth Fund for National Natural Science Foundation of China(Grant No.32001791)the Tangshan Science and Technology Planning Project(Grant No.20150209C)the innovation and entrepreneurship training program for college students of North China University of Science and Technology(Grant No.X2019256).
文摘Chinese mahogany(Toona sinensis) is of considerable medical and economic importance, and its genome has been deciphered. However, the process underlying its polyploidy is unclear, and the chromosomal evolutionary trajectory is poorly understood. Here, by reanalysing the T.sinensis genome, we found evidence of a tetraploidization event(T. sinensis special tetraploidization, TST) that occurred approximately 15-17 million years ago(MYA) after the core eudicot-common hexaploidization(ECH or gamma) event. We characterized the synonymous nucleotide substitution rates(Ks values) of collinear genes and found that T. sinensis genes affected by the TST evolve at a slower rate than Acer yangbiense genes. Furthermore, we identified homologous genes related to polyploidization and speciation and constructed multiple alignments with different reference genomes. Notably, the significant balance of gene retention and loss characterized in the two TST-derived subgenomes suggests an autopolyploid nature of the TST. Moreover, we deduced the chromosomal karyotypes of the two subgenomes and identified 7chromosomal fusions that have shaped the T. sinensis genome;more information is available on a newly constructed karyotype platform(http://www.cgrpoee.top/Toona_sinensis/index.html). The T. sinensis genome preserves the ancestral chromosome structure of dicotyledons well and could serve as a good reference for understanding genomic changes in other Meliaceae and Sapindales plants. In addition, we verified that tandem duplication and the ECH have promoted the expansion of terpene synthase(TPS) genes;conversely, the TST seems to have inhibited expansion of these genes. This present effort has clarified the polyploidy events of the T. sinensis genome, filled gaps in the history of karyotype evolution, and laid a solid foundation for further genomic studies in the Meliaceae research community and beyond.
基金the National Knowledge Innovation of CAS(No.KSCX2-YW-G-038)the Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2015-ZZ09)。
文摘Three new limonoid-type triterpenoids,namely toonasins A–C(1–3)with a rare lactam E ring,along with six known compounds(4–9)were isolated from the barks of Toona sinensis.The structures of new compounds were elucidated by interpretation of spectroscopic data,and the relative configuration of compound 1 was further characterized by X-ray crystallographic analyses.The isolated compounds were evaluated for their cytotoxic activities against five human tumor cell lines(HL-60,SMMC-7721,A-549,MCF-7 and SW480),and compounds 3 and 5 showed weak cytotoxicities.
基金Supported by the Integration and Demonstration of High-efficient Production Technology of Facility Toona sinensis in Mountainous Areas,the Science and Technology Support Program of Shijiazhuang City(141520208A)the Environmental Regulation,Balanced Fertilization and Green Control Technology for Toona Sinensis,the Science and Technology Research and Development Project of Shijiazhuang City(161490282A)
文摘In order to prolong the supply period and increase the economic value of Toona sinensis,the protected cultivation of T. sinensis was carried out on mountainous slope to achieve stagger-season planting and the year round supply. The results showed that in late October to midNovember,the temperature was moderate( 1-10℃) and suitable for the lifting of seedlings and temporary planting dormancy. The line spacing of rooted seedlings in greenhouse was( 22-24) cm ×( 4-5) cm and planting density was 900 000-1 200 000 seedlings/ha. The line spacing of seedlings was( 14-13) cm ×( 3-4) cm and planting density was 1 950 000-2 100 000 seedlings/ha. T. sinensis began to sprout at 8-10℃ and stretch at 10-12℃. When the temperature was stable at 14℃ or above,T. sinensis started bolting and emerging leaves. The terminal buds sprouted in 40-50 d for the T. sinensis planted in greenhouse with the day temperature of 15-22℃ and night temperature over 10℃; the terminal buds sprouted in about 15 d for those planted in the greenhouse with day temperature of 20-25℃ and night temperature over 10℃. T. sinensis could be picked 3 times when planted in greenhouse,and the yield was about 24 720 kg/ha.
