AIM:TT virus (TTV) is a newly described DNA virus related to postransfusion hepatitis that produces persistent viremia in the absence of clinical manifestations.PEG-IFN plus ribavirin have been useful in the treatment...AIM:TT virus (TTV) is a newly described DNA virus related to postransfusion hepatitis that produces persistent viremia in the absence of clinical manifestations.PEG-IFN plus ribavirin have been useful in the treatment of chronic hepatitis C infection.This study investigated the responses of TT virus (TTV) and hepatitis C virus (HCV) to PEG-IFN plus ribavirin therapy. METHODS:Fifteen patients infected with HCV were treated with PEG-IFN(0.5 μg/body weight/week) and ribavirin (1000 mg-1 200 mg/daily) for 48 weeks,Blood samples were drawn at the beginning and the end of the therapy.Serum TTV DNA and HCV RNA were quantified by real time PCR. RESULTS:At the beginning of treatment,TTV infection was detected in 10/15 (66.6%) of HCV-infected patients.Loss of serum TTV DNA at the end of therapy occurred in 6/10 (60%) patients.Out of these 6 patients,4 (67%) became positive for TTV DNA after 6 months of therapy.Regarding HCV viremia,11/15 (73%) patients were negative for serum HCV RNA after 48 weeks of therapy,7/11 (64%) of these cases also became negative for TTV DNA following the combined treatment.In the 3/4 (75%) patients who were positive for HCV RNA at the end of therapy,TTV DNA was detected as well.Sustained HCV response at 6 months after treatment was 53% (8/15). CONCLUSION:No TTV sustained response can be achieved in any patient after PEG-IFN plus ribavirin administration.展开更多
AIM: To investigate the state of infection, replication site, pathogenicity and clinical significance of transfusion transmitted virus (TTV) in patients with hepatitis, especially in patients of unknown etiology. METH...AIM: To investigate the state of infection, replication site, pathogenicity and clinical significance of transfusion transmitted virus (TTV) in patients with hepatitis, especially in patients of unknown etiology. METHODS: Liver tissues taken from 136 cases of non-A non-G hepatitis were tested for TT virus antigen and nucleic acid by in situ hybridization (ISH) and nested-polymerase chain reaction (PCR). Among them, TT virus genome and its complemental strand were also detected in 24 cases of autopsy liver and extrahepatic tissues with ISH. Meanwhile, TTV DNA was detected in the sera of 187 hepatitis patients by nested-PCR. The pathological and clinical data of the cases infected with TTV only were analyzed. RESULTS: In liver, the total positive rate of TTV DNA was 32.4% and the positive signals were located in the nuclei of hepatocytes. In serus, TTV DNA was detected in 21.4% cases of hepatitis A-G, 34.4% of non-A non-G hepatitis and 15% of healthy donors. The correspondence rate of TTV DNA detection between liver tissue with ISH and sera with PCR was 63.2% and 89.3% in the same liver tissues by ISH and by PCR, respectively.Using double-strand probes and single-strand probes designed to detect TTV genome, the correspondence rate of TTV DNA detected in liver and extrahepatic tissues was 85.7%. Using single-strand probes, TTV genome could be detected in liver and extrahepatic tissues by PCR, but its complemental strands (replication strands) could be observed only in livers. The liver function of most cases infected with TTV alone was abnormal and the liver tissues had different pathological damage such as ballooning, acidophilia degeneration, formation of apoptosis bodies and focus of necrosis, but the inflammation in the lobule and portal area was mild. CONCLUSION: The positive rate of TTV DNA among cases of hepatitis was higher than that of donors, especially in patients with non-A non-G hepatitis, but most of them were coinfected with other hepatitis viruses. TTV can infect not only hepatocytes, but also extrahepatic tissues. However, the chief replication place may be liver. The infection of TTV may have some pathogenicity. Although the pathogenicity is comparatively weak, it can still damage the liver tissues. The lesions in acute hepatitis (AH) and chronic hepatitis (CH) are mild, but in severe hepatitis (SH), it can be very serious and cause liver function failure, therefore, we should pay more attention to TTV when studying the possible pathogens of so-called "liver hepatitis of unknown etiology".展开更多
猪输血传播病毒(Torque teno sus virus,TTSuV)是一种单链,无包膜的DNA病毒。在健康的或患有某些疾病的猪中,都能检测到它的存在,具有很高的流行率。目前,针对TTSuV的检测方法有多种,如PCR方法,血清免疫学方法等,但是各有优缺点,因此本...猪输血传播病毒(Torque teno sus virus,TTSuV)是一种单链,无包膜的DNA病毒。在健康的或患有某些疾病的猪中,都能检测到它的存在,具有很高的流行率。目前,针对TTSuV的检测方法有多种,如PCR方法,血清免疫学方法等,但是各有优缺点,因此本文就现有的TTSuV检测方法做一个简单综述,使读者能够对TTSuV的检测方法有一个全面了解。展开更多
目的：建立生物制品中猪细环病毒（ Torque teno sus virus，TTSuV）污染的检测方法，并进行方法学分析及初步的应用。方法针对TTSuV保守序列设计引物和探针，建立荧光PCR方法，对方法的特异性、线性、精密度、最低检测限等参数进行验...目的：建立生物制品中猪细环病毒（ Torque teno sus virus，TTSuV）污染的检测方法，并进行方法学分析及初步的应用。方法针对TTSuV保守序列设计引物和探针，建立荧光PCR方法，对方法的特异性、线性、精密度、最低检测限等参数进行验证，并对试验样本对检测的干扰性进行分析。利用该方法对猪全血样品、生产用细胞及轮状病毒疫苗样品进行检测，通过建立TTSuV分型检测的PCR方法对阳性样品进行分型。结果荧光PCR法的特异性较好，与不同种属的细小病毒、猴SV40病毒及猪圆环病毒无明显的交叉反应，TTSuV1和TTSuV2荧光PCR分别在109～103拷贝／μl和109～102拷贝／μl范围内线性较好，R2值达到0．993以上，TTSuV1和TTSuV2的最低检测限分别为1×103拷贝／μl和1×102拷贝／μl，试验内和试验间的Ct的精密度CV值均小于7％，试验内病毒拷贝数的CV值小于25％，试验间CV值小于45％。细胞样品成分对病毒检测无明显的干扰性。对20份猪全血样品进行检测，8份为阳性，其中1份为TTSuV1阳性，4份为TTSuV2阳性，3份为TTSuV1／2混合感染。 TTSuV1和TTSuV2型与标准株序列同源性分别为98％～99％和98％。对细胞样品和轮状病毒疫苗进行检测，结果均为阴性。结论成功建立了TTSuV荧光PCR检测法，能够用于生物制品TTSuV污染的检测，进一步提高了生物制品的安全性。展开更多
基金Supported by Fundacion Manchega de Investigacion y Docencia en Gastroenterologiapartially by Red Nacional en Investigacin de Hepatologa y Gastroenterologia (RNIHG) Javier MorenoGloria Moraleda contributed equally to this work
文摘AIM:TT virus (TTV) is a newly described DNA virus related to postransfusion hepatitis that produces persistent viremia in the absence of clinical manifestations.PEG-IFN plus ribavirin have been useful in the treatment of chronic hepatitis C infection.This study investigated the responses of TT virus (TTV) and hepatitis C virus (HCV) to PEG-IFN plus ribavirin therapy. METHODS:Fifteen patients infected with HCV were treated with PEG-IFN(0.5 μg/body weight/week) and ribavirin (1000 mg-1 200 mg/daily) for 48 weeks,Blood samples were drawn at the beginning and the end of the therapy.Serum TTV DNA and HCV RNA were quantified by real time PCR. RESULTS:At the beginning of treatment,TTV infection was detected in 10/15 (66.6%) of HCV-infected patients.Loss of serum TTV DNA at the end of therapy occurred in 6/10 (60%) patients.Out of these 6 patients,4 (67%) became positive for TTV DNA after 6 months of therapy.Regarding HCV viremia,11/15 (73%) patients were negative for serum HCV RNA after 48 weeks of therapy,7/11 (64%) of these cases also became negative for TTV DNA following the combined treatment.In the 3/4 (75%) patients who were positive for HCV RNA at the end of therapy,TTV DNA was detected as well.Sustained HCV response at 6 months after treatment was 53% (8/15). CONCLUSION:No TTV sustained response can be achieved in any patient after PEG-IFN plus ribavirin administration.
基金the National Natural Science Foundation of China,No.39900133Beijing Natural Science Foundation,No.7992023
文摘AIM: To investigate the state of infection, replication site, pathogenicity and clinical significance of transfusion transmitted virus (TTV) in patients with hepatitis, especially in patients of unknown etiology. METHODS: Liver tissues taken from 136 cases of non-A non-G hepatitis were tested for TT virus antigen and nucleic acid by in situ hybridization (ISH) and nested-polymerase chain reaction (PCR). Among them, TT virus genome and its complemental strand were also detected in 24 cases of autopsy liver and extrahepatic tissues with ISH. Meanwhile, TTV DNA was detected in the sera of 187 hepatitis patients by nested-PCR. The pathological and clinical data of the cases infected with TTV only were analyzed. RESULTS: In liver, the total positive rate of TTV DNA was 32.4% and the positive signals were located in the nuclei of hepatocytes. In serus, TTV DNA was detected in 21.4% cases of hepatitis A-G, 34.4% of non-A non-G hepatitis and 15% of healthy donors. The correspondence rate of TTV DNA detection between liver tissue with ISH and sera with PCR was 63.2% and 89.3% in the same liver tissues by ISH and by PCR, respectively.Using double-strand probes and single-strand probes designed to detect TTV genome, the correspondence rate of TTV DNA detected in liver and extrahepatic tissues was 85.7%. Using single-strand probes, TTV genome could be detected in liver and extrahepatic tissues by PCR, but its complemental strands (replication strands) could be observed only in livers. The liver function of most cases infected with TTV alone was abnormal and the liver tissues had different pathological damage such as ballooning, acidophilia degeneration, formation of apoptosis bodies and focus of necrosis, but the inflammation in the lobule and portal area was mild. CONCLUSION: The positive rate of TTV DNA among cases of hepatitis was higher than that of donors, especially in patients with non-A non-G hepatitis, but most of them were coinfected with other hepatitis viruses. TTV can infect not only hepatocytes, but also extrahepatic tissues. However, the chief replication place may be liver. The infection of TTV may have some pathogenicity. Although the pathogenicity is comparatively weak, it can still damage the liver tissues. The lesions in acute hepatitis (AH) and chronic hepatitis (CH) are mild, but in severe hepatitis (SH), it can be very serious and cause liver function failure, therefore, we should pay more attention to TTV when studying the possible pathogens of so-called "liver hepatitis of unknown etiology".
文摘猪输血传播病毒(Torque teno sus virus,TTSuV)是一种单链,无包膜的DNA病毒。在健康的或患有某些疾病的猪中,都能检测到它的存在,具有很高的流行率。目前,针对TTSuV的检测方法有多种,如PCR方法,血清免疫学方法等,但是各有优缺点,因此本文就现有的TTSuV检测方法做一个简单综述,使读者能够对TTSuV的检测方法有一个全面了解。
