Patients with primary biliary cirrhosis have increased serum total antioxidant capacity measured with the crocin bleaching assay

AIM： The balance between oxidants and antioxidants can play an important role in the initiation and development of liver diseases. Recently, we have described a new automated method for the determination of total antioxidant capacity （TAC） in human serum and plasma.METHODS： We measured TAC and corrected TAC （CTACabstraction of interactions due to endogenous uric acid,bilirubin and albumin） in 52 patients with chronic liver diseases （41 patients with primary biliary cirrhosis （PBC）,10 patients with chronic hepatitis C and 13 patients with viral HCV cirrhosis） as well as in 10 healthy controls. In 23 PBC patients measurement were also done 6 mo after treatment with ursodeoxycholic acid （UDCA）. The TAC assay was based on a modification of the crocin bleaching assay. The results were correlated with routine laboratory measurements and the histological stage of PBC.RESULTS： There were no significant differences in TAC between the various groups. However, CTAC was considerably increased in the PBC group compared to controls and cirrhotics. Analysis of these patients according to disease stages showed that this increase was an early phenomenon observed only in stages I and II compared to controls, cirrhotics and patients with chronic hepatitis C）.After 6 mo of treatment with UDCA, levels of CTAC decreased to those similar to that of controls.CONCLUSION： Patients in the early stages of PBC present with high levels of corrected total antioxidant capacity and this maybe related to the pathophysiology of the disease. UDCA treatment restores the levels of CTAC to control levels.

Changes of total antioxidant capacity and total oxidant status of aqueous humor in diabetes patients and correlations with diabetic retinopathy

AIM:To measure changes of total oxidant status(TOS)and total antioxidant capacity(TAC) of aqueous humor(AH) in diabetic retinopathy(DR) patients,and to determine if there were any differences in TOS and TAC of AH in diabetic patients without retinopathy compared with non-diabetic patients.·METHODS:One hundred and three eyes of 103patients who were enrolled for cataract surgery were included in this study.Patients were grouped according to presence of diabetes and stage of DR.Prior to cataract surgery,0.1mL to 0.2mL of AH was aspirated and analyzed for TAC and TOS level using a colorimetric method.·RESULTS:TOS levels were highest among proliferative diabetic retinopathy(PDR) patients and lowest in patients with only cataracts.Results were statistically significant between all groups(P 【0.05).Whereas result between diabetic without retinopathy patients and non-proliferative diabetic retinopathy(NPDR) patients was not statistically significant(P =0.757).TAC levels were highest in patients with only cataract and lowest among PDR patients and results were statistically significant between all groups(P 【0.05).·CONCLUSION:Aqueous humor TAC levels are low in diabetic patients and reduced further in DR patients,TOS levels are increased in diabetic patients and this is exacerbated in DR patients.

The effect of fitness level on cardiac autonomic regulation, IL-6,total antioxidant capacity, and muscle damage responses to a single bout of high-intensity interval training

Purpose:The aim of this study was to investigate the influence of the cardiorespiratory fitness level on the response to high-intensity interval training(HIIT)with an individually adjusted running speed of the same relative intensity.The evaluation focused on acute cardiorespiratory response,postexercise cardiac autonomic modulation(heart rate variability(HRV))and biochemical markers of inflammation,oxidative stress,and muscle damage.Methods:Thirty participants were divided into 3 subgroups:well trained,moderately trained,and untrained.All the participants performed30 min HIIT composed of 6×2 min interval exercise with work-to-relief ratio=1 and work intensity 100%of individual velocity at maximal oxygen consumption(VO2max).Acute cardiorespiratory variables,postexercise HRV,lactate,interleukin-6(IL-6),total antioxidant capacity(TAC),creatine kinase,and myoglobin up to 4h after HIIT were monitored.Results:The differences in relatively expressed cardiorespiratory variables(heart rate,VO2)during HIIT were at most moderate,with the most pronounced between-group differences in absolute VO2 values.The disruption of the postexercise HRV was the most pronounced in untrained individuals,and this difference persisted 1 h after HIIT.The highest postexercise IL-6 and TAC concentrations and the lowest changes in creatine kinase and myoglobin were revealed in well-trained individuals.Conclusion:The higher fitness level was associated with the less pronounced postexercise cardiac autonomic changes and their faster restoration,even when there were similar acute cardiorespiratory responses.These findings were simultaneously accompanied by the higher postexercise IL-6 and TAC concentrations and less significant changes in muscle damage biochemical markers in well-trained individuals.

Dynamic Analysis of Nitric Oxide and Total Oxidant Capacity in Cow Uterine Secretion with Subclinical Endometritis

Subclinical endometritis is a physiological inflammation that serves to clear persistent contaminants from the uterus. To investigate the alteration of antioxidant, such as vitamin E（VE） and vitamin C（VC）, total oxidant capacity（TOC） and nitric oxide（NO） in cows with normal and subclinical endometritis（SCE）, we examined the concentrations of NO, VC and VE, TOC and polymorphonuclear neutrophils（PMN） percentage in uterine secretion. The cows were divided into two groups, normal（n=20） and subclinical endometritis（SCE, n=60）, based on endometrial cytology（presence of PMN≥5%）. Uterine secretion and blood were collected as described previously. Griess reaction was used to determine the concentration of NO. The concentrations of TOC, VC and VE were detected by a commercially available assay kit. The results showed that the concentrations of NO, TOC and PMN percentage were significantly higher（P〈0.01, P〈0.05, and P〈0.01, respectively） in uterine secretion with SCE compared to those from normal; however, the levels of VC and VE were significantly lesser（P〈0.01）. In conclusion, the concentrations of NO, TOC, VC, VE and PMN percentage differed between normal and SCE cows. Meanwhile, the relationship between the concentration of NO and PMN percentage from uterine secretion in cows with subclinical endometritis were positively correlated. Consequently, these alterations in NO, TOC, VC, VE levels and PMN percentage contributed to as a diagnostic index of the uterine inflammation, with the aim to increase the reproduction of the cows and the decrease economic losses.

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main reducing substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the color of the reagent, which is determined photometrically at 630 nm. This new method quantifies the overall capacity of the sample to reduce a redox chromogen and it requires the minimum volumes and time. The assay can be used on a wide variety of biological samples including whole blood, serum, plasma, tissue extracts and homogenates, food, wine, fruit juice and other beverages. Results are expressed in terms of mmol/L glutathione equivalents. TAC can be assayed manually, on automatic analyzers or micro-plate readers.

Variability in total antioxidant capacity, antioxidant leaf pigments and foliage yield of vegetable amaranth

Twenty vegetable amaranth genotypes were evaluated for total antioxidant capacity, antioxidant leaf pigments, vitamins, and selection of suitable genotypes for extraction of juice in a randomized complete block design（RCBD） with three replications. Vegetable amaranth was rich in chlorophyll, β-cyanins, β-xanthins, betalains, carotene, ascorbic acid and total antioxidant. The genotypes VA14, VA16, VA18, VA15, and VA20 could be selected as amaranth vegtable varieties with high yields and abundance antioxidant leaf pigments and vitamins to produce juice. The genotypes VA13 and VA19 had above-average foliage yield and high antioxidant profiles while the genotypes VA2, VA3, VA9, VA11, VA12, and VA17 had a high antioxidant profiles and below-average foliage yield. These genotypes could be used as a donor parent for integration of potential high antioxidant profiles genes into other genotypes. The correlation study revealed a strong positive association among all the antioxidant leaf pigments, total antioxidant capacity and foliage yield. Selection based on total antioxidant capacity, antioxidant leaf pigments could economically viable to improve the yield potential of vegetable amaranth genotypes. Total carotene and ascorbic acid exhibited insignificant genotypic correlation with all the traits except total antioxidant capacity. This indicates that selection for antioxidant vitamins might be possible without compromising yield loss.

Effect of Colchicine on Total Antioxidant Capacity,Antioxidant Enzymes and Oxidative Stress Markers in Patients with Knee Osteoarthritis

Background/Aim: We aimed to investigate the effects of colchicine on clinical recovery, as well as oxidative stress markers and total antioxidant capacity (TAC) in whole blood of patients with knee osteoarthritis (OA). Materials and methods: Sixty patients with grade 2 - 3 knee OA according to ACR knee OA criteria whom examination of the knee joint synovial fluid by polarized light microscopy demonstrated CPDD crystals existence were included in the study. Fifty healthy subjects were included as a control group. Patients were divided randomly into two groups. The first group (paracetamol group) was given only paracetamol 3 gr daily p.o and the second group (colchicine and paracetamol group) was given colchicine 1,5 gr and paracetamol 3 gr daily p.o for six months. For outcome measures WOMAC and VAS were used. Superoxide dismutase (SOD), Catalase (CAT) ezyme activities and Glutathione (GSH) and Malondialdehyde (MDA) levels and TAC all were measured. Results: WOMAC scores were improved in both patient groups compared with pre-treatment evaluation (p 0.05). TAC was signifcantly increased only in colchicine/paracetamol group. Oxidant parameter MDA levels were significantly decreased in both paracetamol group and colchicine/paracetamol group. CAT, SOD enzyme activities and GSH levels did not change before and after treatment protocols in both patient groups. Conclusion: Both paracetamol 3000 mg/day and 3000 mg paracetamol plus 1,5 gr/day colchicine is effective in the treatment of patients with knee osteoarthritis. But the addition of colchicine to paracetamol produced significantly greater symptomatic benefit than paracetamol alone. Our study also showed that colchicine lowers whole blood MDA which is a lipid peroxidation compound and elevates TAC levels in patients with knee OA. This may show probable disease modifying effect of colchicine in knee OA which require further long period laboratory and radiologic investigations.

血清小而密低密度脂蛋白胆固醇和总抗氧化能力水平与脑梗死患者认知功能障碍的相关性分析

目的:探究脑梗死患者血清小而密低密度脂蛋白胆固醇(small dense low-density lipoprotein cholesterol, sdLDL-C)和总抗氧化能力(total antioxidant capacity, T-AOC)水平变化及其与认知功能障碍发生的关系。方法:选择148例脑梗死患者,依据发病后7 d是否出现认知功能障碍,分为非障碍组(n=77)和障碍组(n=71);分别采用过氧化氢酶法和比色法测定两组血清sdLDL-C和T-AOC水平,并进行比较;Pearson相关系数法分析脑梗死患者血清sdLDL-C、T-AOC水平与蒙特利尔认知评估(MoCA)评分相关性;二元Logistic回归分析影响脑梗死患者认知功能障碍发生的危险因素。结果:与非障碍组相比,障碍组血清sdLDL-C水平明显升高(P<0.05),T-AOC水平明显降低(P<0.05)。相关性分析显示,血清sdLDL-C水平与MoCA评分呈负相关(r=-0.516,P<0.05),T-AOC水平与MoCA评分呈正相关(r=0.446,P<0.05)。Logistic回归分析显示,高血压、糖尿病、病情严重程度、血清sdLDL-C和T-AOC是脑梗死患者认知功能障碍发生的独立影响因素(P<0.05)。结论:脑梗死患者血清sdLDL-C水平升高,T-AOC水平降低,二者是影响脑梗死患者并发认知功能障碍的独立影响因素。

发酵全混合日粮的发酵品质及其对鲁西黑头羊生长性能和抗氧化指标的影响

试验旨在探究发酵全混合日粮(FTMR)的发酵品质及其对鲁西黑头羊生长性能和抗氧化指标的影响。试验以花生秧为粗饲料,与精料按照4∶6混合,发酵30 d制备FTMR,测定其营养成分和发酵品质。选用健康、体重(20.76±1.56)kg的鲁西黑头羊32只,随机分为2组,每组4个重复,每个重复4只羊。对照组饲喂全混合日粮(TMR),试验组饲喂FTMR。预试期15 d,正式试验期63 d,测定其生长性能、养分表观消化率和血清抗氧化指标。结果表明:随着发酵时间延长,FTMR中水分和粗蛋白含量均呈先增加后逐步稳定的趋势,粗灰分含量呈先降低后逐步稳定的趋势;pH值呈先降低后趋于稳定的趋势,乙酸(AA)和丙酸(PA)含量呈先增加后稳定的趋势,氨态氮(NH3-N)含量显著增加(P<0.05)。整个发酵过程中未检出丁酸(BA),玉米赤霉烯酮(ZEN)、黄曲霉毒素B1(AFB1)和呕吐毒素(DON)含量均低于国家标准。与对照组相比,试验组鲁西黑头羊平均日干物质采食量显著降低(P<0.05);试验组鲁西黑头羊对粗蛋白、中性洗涤纤维、酸性洗涤纤维的表观消化率显著提高(P<0.05),干物质的表观消化率显著降低(P<0.05);试验组鲁西黑头羊血清丙二醛(MDA)含量显著降低(P<0.05),总抗氧化能力(T-AOC)显著升高(P<0.05)。研究表明,FTMR发酵品质优良,与TMR相比,饲喂FTMR能够提高鲁西黑头羊生长性能和抗氧化能力。

仿野生种植三叶青不同部位总黄酮分析及其抗炎、抗氧化能力比较

目的:比较仿野生种植三叶青不同部位中总黄酮含量、抗氧化活性以及抗炎能力差异。方法:采用醇提工艺对仿野生种植三叶青茎叶、块根、根须进行提取,比较不同部位间总黄酮含量差异;通过DPPH自由基、ABTS^(+)自由基、羟自由基清除实验以及铁离子还原力测定,比较仿野生种植三叶青不同部位间抗氧化能力的强弱;采用脂多糖(LPS)诱导RAW264.7细胞作为炎症模型,通过细胞NO的释放量比较仿野生种植三叶青不同部位间的抗炎能力差异。结果:在最佳提取工艺条件下,测得仿野生种植三叶青茎叶、块根、根须总黄酮含量分别为11.86±0.23、8.48±0.10、7.52±0.02 mg·g^(-1);建立三叶青HPLC指纹图谱,共标定了10个共有峰,通过对照品指认出6号峰为芦丁、8号峰为槲皮素、9号峰为山奈酚,并对其含量进行检测,结果显示块根中三种指标成分含量均最高,其次根须,茎叶最低。检测仿野生种植三叶青不同部位对DPPH自由基、ABTS^(+)自由基以及羟自由基的IC_(50)值,茎叶分别为0.2107、0.2315、0.7625 mg/mL;块根分别为0.3134、0.3758、0.8967 mg/mL;根须分别为0.2058、0.2587、0.7856 mg/mL。三叶青茎叶、块根、根须对铁离子还原能力的吸光度值分别为0.172、0.153、0.184。当仿野生种植三叶青三部位的质量浓度在25~200μg/mL之间时,它们不会对RAW264.7细胞造成毒性,能够有效地抑制LPS诱导的NO释放,从而达到良好的抗炎效果。结论:本研究结果为仿野生种植三叶青不同部位的质量评价提供参考,并对仿野生种植三叶青非药用部位废物再利用及后续全资源开发提供理论依据。

响应面法优化沙枣叶多酚提取工艺及体内外抗氧化活性研究

采用单因素实验和响应面法优化了新疆沙枣叶多酚的超声辅助提取工艺,并通过测定沙枣叶多酚对DPPH自由基的清除能力及对小鼠血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)的影响评价其抗氧化活性。结果表明,沙枣叶多酚的最佳提取工艺为:料液比1∶40(g∶mL)、乙醇体积分数58%、提取时间21 min、提取功率250 W,在此条件下,多酚含量为36.45 mg·g^(-1);当浓度为0.20 mg·mL^(-1)时,沙枣叶多酚和VC对DPPH自由基的清除率分别为90.3%、96.5%;与空白对照组比较,模型对照组小鼠血清中MDA水平极显著升高(P<0.01),SOD、GSH-Px、T-AOC的水平均极显著降低(P<0.01),且模型对照组的肾脏系数和肝脏系数与空白对照组差异极显著(P<0.01);与模型对照组比较,沙枣叶多酚高剂量组血清中MDA水平极显著降低(P<0.01),SOD、GSH-Px、T-AOC的水平均极显著升高(P<0.01),且肾脏系数和肝脏系数与模型对照组无显著差异(P>0.05)。

黑苦荞固态发酵富集总酚、总黄酮工艺优化及其抗氧化活性研究

探究固态发酵促进黑苦荞生物活性成分多酚和黄酮的高效释放,并提高其抗氧化活性。以总酚、总黄酮含量为评价指标,先筛选发酵菌种,然后以发酵时间、装料量、接种量为变量,采用单因素和响应面试验对黑苦荞固态发酵工艺进行优化,分析在最优菌种及最佳发酵条件下固态发酵对黑苦荞营养成分及体外抗氧化活性的影响。结果表明:最优发酵菌种为米曲霉,固态发酵黑苦荞最佳工艺条件为接种量11.0 mL/100 g、装料量28.6 g、发酵时间3.6 d,在此条件下,总酚和总黄酮含量最高,为(27.00±0.43)mg/g和(2.50±0.02)mg/g,分别是空白组、对照组的2.61、2.72倍和2.02、2.16倍,淀粉含量显著降低,蛋白质、脂肪和灰分含量显著升高(P<0.05);发酵黑苦荞多酚清除DPPH和ABTS自由基的IC50((33.08±2.21)μg/mL、(16.41±2.21)μg/mL)显著低于发酵前((50.67±3.13)μg/mL、(28.07±3.42)μg/mL),总抗氧化和总还原能力显著提高(P<0.05)。固态发酵可以显著提高黑苦荞总酚、总黄酮含量和抗氧化活性,为开发黑苦荞功能性食品提供理论依据。

Cd^(2+)对毛蚶的毒性效应及对其SOD活性和TAOC的影响

在实验室条件下研究了Cd Cl2对毛蚶的急性毒性效应及对其超氧化物歧化酶(SOD)活性和总抗氧化能力(TAOC)的影响.Cd Cl2对毛蚶24、48、72、96,h的半致死浓度(LC50)分别为24.88、14.52、10.54、6.20,mg/L,随着暴露时间的增加LC50逐渐变小,说明Cd2+对毛蚶的毒性逐渐增加,毛蚶长时间暴露在较低质量浓度的Cd Cl2溶液或短时间暴露在高质量浓度的Cd Cl2溶液中均可产生致死效应.同时测定了不同质量浓度Cd2+(100、500,μg/L)胁迫9,d和清洁海水恢复后毛蚶体内SOD活性和TAOC值的变化,毛蚶体内SOD活性和TAOC值总体被诱导,SOD活性在第9天达到最大值,TAOC诱导率在染毒第7天达到峰值,随后下降.消除Cd2+的胁迫进行清水恢复后,毛蚶SOD活性降低,TAOC变化不明显(P>0.05),表明Cd2+对毛蚶的氧化胁迫作用不会在短时间之内得到恢复.

黑牛肝菌多糖组分对其抗氧化能力的贡献研究

从黑牛肝菌中提取出的多糖可用于美容、医疗、保健等。将晾干后的黑牛肝菌进行脱脂处理,通过超声波辅助提取、传统热水提取和强碱提取,分级提取出黑牛菌中的粗多糖。对粗多糖进行脱色处理后,检测多糖含量和纯度以及单糖组成;然后采用紫外、红外和三维螺旋结构分析等方法对多糖结构进行分析;最后采用铁氰化钾显色法和水杨酸显色法对多糖和五种组成单糖标准样品对Fe^(3+)的还原力和对羟基自由基的清除率进行测定。结果表明,三步分级提取出的粗多糖含量不同,单糖种类有一定差异,但纯度都较高,都具有多糖的结构特征,都具有良好的抗氧化性能;对Fe^(3+)的还原力主要由其组分甘露糖和半乳糖贡献,但组成多糖对氧自由基的清除能力贡献不大。

温针对大鼠糖尿病周围神经病变坐骨神经传导速度及MDA、SOD、TAOC的作用

目的运用温针治疗糖尿病周围神经病变(Diabetic Peripheral Neuropathy)模型大鼠,观察该法对大鼠坐骨神经传导速度以及氧化和抗氧化能力的影响,从神经保护的角度探讨温针治疗DPN的可能机制。方法链尿佐菌素(Streptozotocin,STZ)肌肉注射诱导形成糖尿病周围神经病变模型,随机分为模型组、弥可保组、温针组。温针组每日针刺大鼠双侧脾俞、肾俞、环跳和后三里穴,得气后在针尾缠绕艾绒点燃施灸30 min,弥可保组每日肌肉注射弥可保50μg/kg。另设正常组进行对照,模型组和正常组只作捆绑处理。共治疗8星期。分别于治疗前及治疗后观察一般状态、血糖,测定坐骨神经传导速度,用化学比色法测定坐骨神经丙二醛(MDA)、超氧化物歧化酶(SOD)和总抗氧化能力(TAOC)。结果治疗后,弥可保组和温针组坐骨神经感觉传导速度明显优于模型组(P<0.01),MDA水平明显低于模型组(P<0.01),SOD和TAOC水平明显高于模型组(P<0.01)。而温针组与弥可保组相比较,以上各项亦有明显差异(P<0.05)。结论温针能有效提高DPN模型大鼠神经传导速度,并且能有效地减轻氧化应激对神经的损伤程度。

响应面法优化3味山姜属中药挥发油提取工艺及总抗氧化能力研究

目的优化高良姜、大高良姜、红豆蔻3味山姜属中药的挥发油提取工艺,并研究其体外抗氧化能力。方法基于单因素实验结果,以3味山姜属中药挥发油得率为指标,料液比、浸泡时间、提取时间为考察因素,水蒸气蒸馏法为提取方法,采用Box-Behnken响应面法确定3味山姜属中药挥发油的最优提取条件;采用FRAP法与ABTS自由基清除法对3味山姜属中药挥发油进行体外总抗氧化能力测定。结果高良姜挥发油最优提取工艺为料液比1∶10.4,浸泡时间31 min,提取时间6.2 h;大高良姜挥发油最优提取工艺为料液比1∶12.4,浸泡时间84 min,提取时间7.3 h;红豆蔻挥发油最优提取工艺为料液比1∶12.3,浸泡时间90 min,提取时间6.5 h。相同浓度下,3味山姜属中药挥发油Fe3+还原能力排序为红豆蔻>大高良姜≈高良姜,Trolox当量总抗氧化能力排序为红豆蔻≈大高良姜>高良姜。结论Box-Behnken响应面法操作简便,优化结果直观,预测准确;所得3味山姜属中药挥发油提取最优工艺方案稳定性高,重复性好;3味山姜属中药挥发油具有较好的体外抗氧化活性。

产褥期感染危险因素分析及TAC、IFN-γ及α1-AG预测价值

目的:分析产褥期感染危险因素并探讨总抗氧化能力(TAC)、干扰素γ(IFN-γ)及α1酸性糖蛋白(α1-AG)预测价值。方法:回顾性收集2020年6月-2023年1月本院接诊的250例产妇临床资料,根据产褥期感染发生情况分为感染组(n=31)和未感染组(n=219),收集临床资料,分析产褥期感染危险因素,检测血清TAC、IFN-γ、α1-AG水平并采用受试者工作特征曲线(ROC)分析其预测产褥期感染价值。结果:单因素分析,两组年龄、孕周、产次、内分泌疾病比较无差异(P>0.05);两组产程、妇科炎症、妊娠高血压疾病、妊娠期糖尿病、产后出血、居住地、分娩方式、胎膜早破、卧床时间比较有差异(P<0.05);感染组血清IFN-γ(150.05±36.25 pg/ml)、α1-AG(156.35±40.91 mg/dl)高于未感染组(134.71±30.56 pg/ml、116.25±38.36 mg/dl),TAC(8.20±1.50 kU/ml)低于未感染组(10.83±2.45 kU/ml)(均P<0.05);白细胞计数、C反应蛋白及降钙素原水平产前两组无差异(P>0.05),产后两组均升高且感染组高于未感染组(P<0.05)。多因素非条件logistic分析,产程长、妇科炎症、妊娠高血压疾病、妊娠期糖尿病、产后出血、居住地为乡村、分娩方式为剖宫产、胎膜早破、卧床时间≥3d、TAC降低、IFN-γ升高及α1-AG升高均是产褥期感染发生的独立危险因素(P<0.05);ROC结果显示,预测产褥期感染血清TAC的曲线下面积(AUC)为0.808,灵敏度76.3%,特异度79.5,截断值9.56 kU/ml;血清IFN-γ的AUC为0.651,灵敏度61.5%,特异度69.4%,截断值141.24 pg/ml;血清α1-AG的AUC为0.753,灵敏度73.4%,特异度70.8%,截断值130.35 mg/dl。结论:产程长、妇科炎症、妊娠高血压疾病、妊娠期糖尿病、产后出血、居住地为乡村、剖宫产、胎膜早破、卧床时间长均是产褥期感染的独立危险因素,血清TAC、IFN-γ、α1-AG在产褥期感染者血清中表达异常且可对产褥期感染的发生有一定预测价值。

基于Fe-HHTP@ZIF-8构建可视化传感器测定饮料的总抗氧化能力

食品的总抗氧化能力是评价其功效的关键指标之一,建立快速灵敏的分析方法用于食品总抗氧化能力检测具有十分重要的意义。本文首次设计合成了一种具有类过氧化物酶活性的金属-有机框架复合物纳米酶(FeHHTP@ZIF-8),并对其形貌、结构及催化活性进行了详细表征。结果表明,Fe-HHTP@ZIF-8是一种具有二维超薄片层结构的纳米酶,计算得到Fe-HHTP@ZIF-8纳米酶对H_(2)O_(2)的Km和Vmax分别为0.67 mmol/L和3.55×10^(-8)mol/L·s^(-1)。进一步以抗坏血酸(Ascorbic acid,AA)作为典型抗氧化剂,基于其对纳米酶催化显色反应的抑制作用,构建了一种高效的可视化传感器方法。系统验证表明,该可视化传感器检测限低(1.67μmol/L),线性范围宽(5.0~3000.0μmol/L),准确度高(回收率为98.62%~103.13%)。利用此传感器对不同饮料的总抗氧化能力进行检测,结果与样品标示值基本一致。因此,本研究为食品总抗氧化能力的评价提供了一种简单、快捷、有效的可视化新方法。

天然抗氧化剂对辣椒鸡油抗氧化性能的影响

为了筛选出适合辣椒鸡油的天然抗氧化剂,以延长其保质期,研究并比较了6种天然抗氧化剂(迷迭香提取物、维生素E、茶多酚、维生素C、槲皮素和硫辛酸)对辣椒鸡油酸值、过氧化值、DPPH自由基清除能力和总还原能力的影响。结果表明:6种天然抗氧化剂在辣椒鸡油中均具有一定的抗氧化能力;在抑制酸值方面,槲皮素效果最佳,维生素C最差;在抑制过氧化值方面,槲皮素效果最佳,维生素E最差;在DPPH自由基清除能力方面,迷迭香提取物效果最佳,硫辛酸最差;在总还原能力方面,槲皮素效果最佳,维生素E最差;槲皮素、迷迭香提取物和茶多酚在酸值、过氧化值、DPPH自由基清除能力和总还原能力方面均位于前三。综合考虑抗氧化效果、抗氧化剂稳定性、成本等因素,可优先选择迷迭香提取物和茶多酚作为辣椒鸡油的抗氧化剂以提高其储藏性能。

云南主栽核桃和油橄榄16个品种油脂体外抗氧化活性研究

分析云南主栽核桃和油橄榄品种的体外抗氧化活性,为生产核桃和油橄榄的高端复配油提供理论依据。采用试剂盒法对液压榨取核桃油及西班牙橄榄油分析检测系统制备橄榄油脂的总抗氧化能力、羟自由基清除能力、ABTS自由基清除能力、DPPH自由基清除能力以及超氧阴离子清除能力进行分析。结果显示:8个主栽核桃品种的核桃油总抗氧化能力在1.70~10.18(μmol Trolox)·g^(-1)之间,ABTS自由基清除能力在264.04~2224.39(μg Trolox)·g^(-1)之间,DPPH自由基清除能力在388.62~2892.73(μg Trolox)·g^(-1)之间,羟自由基清除率在52.88%~77.77%之间,超氧阴离子清除率在16.25%~39.08%之间,总抗氧化能力、ABTS自由基清除能力及DPPH自由基清除能力品种间差异较大;8个品种油橄榄果油总抗氧化能力在0.73~4.20(μmol Trolox)·g^(-1)之间,ABTS自由基清除能力在153.57~610.91(μg Trolox)·g^(-1)之间,DPPH自由基清除能力在79.20~878.67(μg Trolox)·g^(-1)之间,羟自由基清除率在57.41%~62.83%之间,超氧阴离子清除率在16.69%~38.36%之间。结果表明:云南主栽8个核桃品种抗氧化性能最好品种为‘大姚三台核桃’,其次为‘细香核桃’,抗氧化性能与总酚含量呈显著正相关;油橄榄油脂体外抗氧化活性低于核桃油,其中抗氧化性能最好品种为‘金叶佛樨榄’,其次为‘科拉蒂’,抗氧化性能与总酚含量也呈显著正相关。