Association of total bilirubin with depression risk in adults with diabetes:A cross-sectional study

BACKGROUND Individuals with diabetes mellitus are more likely to experience depression,although most patients remain undiagnosed.The relation between total bilirubin and depression has been increasingly discussed,but limited studies have examined the association of total bilirubin with depression risk in adults with diabetes,which warrants attention.AIM To investigate the association between total bilirubin levels and the risk of depression in adults with diabetes.METHODS The study included adults with diabetes from the National Health and Nutrition Examination Survey 2007-2018.Depression was determined using the Patient Health Questionnaire-9.Multivariable logistic regression,propensity scorematched analysis and restricted cubic spline models were utilized to investigate the association between total bilirubin levels and depression risk in adults with diabetes.RESULTS The study included 4758 adults with diabetes,of whom 602(12.7%)were diagnosed with depression.After adjusting for covariates,we found that diabetic adults with lower total bilirubin levels had a higher risk of depression(OR=1.230,95%CI:1.006-1.503,P=0.043).This association was further confirmed after propensity score matching(OR=1.303,95%CI:1.034-1.641,P=0.025).Subgroup analyses showed no significant dependence of age,body mass index,sex,race or hypertension on this association.Restricted cubic spline models displayed an inverted U-shaped association of total bilirubin levels with depression risk within the lower range of total bilirubin levels.The depression risk heightened with the increasing levels of total bilirubin,reaching the highest risk at 6.81μmol/L and decreasing thereafter.CONCLUSION In adults with diabetes,those with lower levels of total bilirubin were more likely to have depressive symptoms.Serum total bilirubin levels may be used as an additional indicator to assess depression risk in adults with diabetes.

Better performance of PIVKA-II for detecting hepatocellular carcinoma in patients with chronic liver disease with normal total bilirubin

BACKGROUND Serum protein induced by vitamin K absence or antagonist-Ⅱ(PIVKA-Ⅱ) is a promising biomarker for hepatocellular carcinoma(HCC) surveillance.AIM To identify the contributing factors related to the abnormal elevation of PIVKA-Ⅱ level and assess their potential influence on the performance of PIVKA-Ⅱ in detecting HCC.METHODS This study retrospectively enrolled in 784 chronic liver disease(CLD) patients and 267 HCC patients in Mengchao Hepatobiliary Hospital of Fujian Medical University from April 2016 to December 2019. Logistic regression and the area under the receiver operating characteristic curve(AUC) were used to evaluate the influencing factors and diagnostic performance of PIVKA-Ⅱ for HCC, respectively.RESULTS Elevated PIVKA-Ⅱ levels were independently positively associated with alcohol-related liver disease, serum alkaline phosphatase(ALP), and total bilirubin(TBIL) for CLD patients and aspartate aminotransferase(AST) and tumor size for HCC patients(all P < 0.05). Serum PIVKA-Ⅱ were significantly lower in patients with viral etiology, ALP ≤ 1 × upper limit of normal(ULN), TBIL ≤ 1 × ULN, and AST ≤ 1 × ULN than in those with nonviral disease and abnormal ALP, TBIL, or AST(all P < 0.05), but the differences disappeared in patients with early-stage HCC. For patients with TBIL ≤ 1 × ULN, the AUC of PIVKA-Ⅱ was significantly higher compared to that in patients with TBIL > 1 × ULN(0.817 vs 0.669, P = 0.015), while the difference between ALP ≤ 1 × ULN and ALP > 1 × ULN was not statistically significant(0.783 vs 0.729, P = 0.398). These trends were then more prominently perceived in subgroups of patients with viral etiology and HBV alone.CONCLUSION Serum PIVKA-Ⅱ has better performance in detecting HCC at an early stage for CLD patients with normal serum TBIL.

Predictive value of bilirubin and serum γ-glutamyltranspeptidase levels in type-2 diabetes mellitus patients with acute coronary syndrome

BACKGROUND Cardiovascular disease is a major complication of diabetes mellitus(DM).Type-2 DM(T2DM)is associated with an increased risk of cardiovascular events and mortality,while serum biomarkers may facilitate the prediction of these outcomes.Early differential diagnosis of T2DM complicated with acute coronary syndrome(ACS)plays an important role in controlling disease progression and improving safety.AIM To investigate the correlation of serum bilirubin andγ-glutamyltranspeptidase(γ-GGT)with major adverse cardiovascular events(MACEs)in T2DM patients with ACS.METHODS The clinical data of inpatients from January 2022 to December 2022 were analyzed retrospectively.According to different conditions,they were divided into the T2DM complicated with ACS group(T2DM+ACS,n=96),simple T2DM group(T2DM,n=85),and simple ACS group(ACS,n=90).The clinical data and laboratory indices were compared among the three groups,and the correlations of serum total bilirubin(TBIL)levels and serumγ-GGT levels with other indices were discussed.T2DM+ACS patients received a 90-day follow-up after discharge and were divided into event(n=15)and nonevent(n=81)groups according to the occurrence of MACEs;Univariate and multivariate analyses were further used to screen the independent influencing factors of MACEs in patients.RESULTS The T2DM+ACS group showed higherγ-GGT,total cholesterol,low-density lipoprotein cholesterol(LDL-C)and glycosylated hemoglobin(HbA1c)and lower TBIL and high-density lipoprotein cholesterol levels than the T2DM and ACS groups(P<0.05).Based on univariate analysis,the event and nonevent groups were significantly different in age(t=3.3612,P=0.0011),TBIL level(t=3.0742,P=0.0028),γ-GGT level(t=2.6887,P=0.0085),LDL-C level(t=2.0816,P=0.0401),HbA1c level(t=2.7862,P=0.0065)and left ventricular ejection fraction(LEVF)levels(t=3.2047,P=0.0018).Multivariate logistic regression analysis further identified that TBIL level and LEVF level were protective factor for MACEs,and age andγ-GGT level were risk factors(P<0.05).CONCLUSION Serum TBIL levels are decreased andγ-GGT levels are increased in T2DM+ACS patients,and the two indices are significantly negatively correlated.TBIL andγ-GGT are independent influencing factors for MACEs in such patients.

AFP联合ALT和T-Bil检测在肝细胞肝癌中的诊断价值

目的探讨肿瘤标志物甲胎蛋白(AFP)在肝细胞肝癌(HCC)患者血清中的表达水平及其联合ALT和T-Bil检测在HCC诊断中的应用价值。方法选取乐山市中医医院2017年1月~2019年12月确诊为肝细胞肝癌的初诊患者552例(HCC组),其中早期HCC患者196例(早期HCC组),另选取肝脏良性疾病1857例作为对照组,检测和比较HCC患者和对照组患者血清AFP、ALT和T-Bil水平,以AFP/(ALT×T-Bil)作为AFP联合ALT和T-Bil诊断HCC的联合诊断方式,运用受试者工作特征(ROC)曲线分析和比较AFP和联合诊断方式对HCC的诊断效能。结果HCC组患者血清AFP水平明显高于对照组(P<0.001),而血清ALT和T-Bil水平均明显低于对照组(P<0.001)。AFP血清水平和联合诊断方式计算结果与HCC的肿瘤大小呈明显的正相关(r=0.325,P<0.001;r=0.217,P<0.001)。联合诊断方式诊断HCC和早期HCC的ROC曲线下面积(AUROC)分别为0.869(95%CI:0.852~0.886)和0.841(95%CI:0.812~0.870),均明显高于AFP的0.782(95%CI:0.758~0.806)和0.741(95%CI:0.702~0.779)(P<0.001)。以AFP≥20 ng/ml肝脏良性疾病人群为对照,联合诊断方式诊断AFP≥20 ng/ml的HCC和早期HCC的AUROC分别为0.960(95%CI:0.949~0.972)和0.945(95%CI:0.924~0.964),均明显高于AFP的0.805(95%CI:0.773~0.837)和0.734(95%CI:0.673~0.795)(P<0.001)。结论联合诊断方式适用于HCC的诊断,尤其在诊断AFP≥20 ng/ml的HCC和早期HCC时,联合诊断方式的诊断价值能得到进一步的提高。

Higher plasma bilirubin predicts veno-occlusive disease in early childhood undergoing hematopoietic stem cell transplantation with cyclosporine

AIM: To analyze the association between plasma bilirubin levels and veno-occlusive disease(VOD) in non-adult patients undergoing hematopoietic stem cell transplantation(HSCT) during cyclosporine therapy.METHODS: A total of 123 patients taking cyclosporinewere evaluated using an electronic medical system at the Seoul National University Children's Hospital from the years 2004 through 2011. Patients were grouped by age and analyzed for incidence and type of adverse drug reactions(ADRs) including VOD. RESULTS: The HSCT patients were divided into three age groups: G#1 ≥ 18; 9 ≤ G#2 ≤ 17; and G#3 ≤ 8 years of age). The majority of transplant donor types were cord blood transplantations. Most prevalent ADRs represented acute graft-vs-host disease(a GVHD) and VOD. Although the incidences of a GVHD did not vary among the groups, the higher frequency ratios of VOD in G#3 suggested that an age of 8 or younger is a risk factor for developing VOD in HSCT patients. After cyclosporine therapy, the trough plasma concentrations of cyclosporine were lower in G#3 than in G#1, indicative of its increased clearance. Moreover, in G#3 only, a maximal total bilirubin level(BILmax) of ≥ 1.4 mg/d L correlated with VOD incidence after cyclosporine therapy. CONCLUSION: HSCT patients 8 years of age or younger are more at risk for developing VOD, diagnosed as hyperbilirubinemia, tender hepatomegaly, and ascites/weight gain after cyclosporine therapy, which may be represented by a criterion of plasma BILmax being ≥ 1.4 mg/d L, suggestive of more sensitive VOD indication in this age group.

Correlation between Transcutaneous Bilirubinemia and Blood Bilirubinemia in Screening Term Newborn for Neonatal Jaundice at the Essos Hospital Centre (EHC), Yaoundé, Cameroon

Background: Early and non-invasive diagnosis of neonatal hyperbilirubinemia remains critical in dark skinned babies of low resource settings. Objective: To assess correlation/agreement between transcutaneous bilirubin (Tcb) and serum bilirubin (Tsb) values in full term neonates with jaundice. Methodology: An analytical cross-sectional study was conducted at the neonatology unit of the Essos Hospital Centre (EHC) from January to June 2019. All full-term neonates aged 0 to 7 days with suspected jaundice who did not receive phototherapy were eligible for the study. The enrolled neonates in the study were assessed clinically, then with the MBJ20 transcutaneous bilirubinometer (TcB). The MBJ20 transcutaneous bilirubinometer highest measurement over the forehead and the sternum were compared to TsB. Data were entered and then analysed with the CsPro7.2 and R (version 3.6.0) software. Correlation was captured by Bland & Alman plots and Concordance Correlation Coefficient (CCC) estimates. The Pearson correlation coefficient and Student test for paired data were used for descriptions purposes, and the significance level was 5%. Results: We recruited 88 neonates. The sex ratio of the babies included was 1.25 favouring males. Median Post-natal age was 3 days with 62% aged 72 hours or more. The mean TcB corresponding to the maximum average between frontal and sternal measurement was 153 mg/dl ± 48 and the average Tsb was 123.80 mg/dl ± 50.48. A good linear correlation was found between TcB and total serum bilirubin level r = 0.86 [0.80;0.91]. Positive correlation was noted between both (forehead and sternum) TcB measurements sites, namely r = 0.78 and r = 0.86. The Bland & Altman plot measured the bias at -29.68 mg/l (confidence interval at 95%, 21.14 - 80.50). The CCC estimate was 0.2 varying from -0.22 to 0.76 according to TcB measurement threshold and post-natal age. The ROC area under the curve value for a threshold < 100 mg/l equals 90% proving to be a good predictor for this threshold. Conclusion: A good linear correlation was found despite a poor agreement between TcB and Tsb. TcB method systematically overestimated the value of TsB.

肝素结合蛋白、总胆红素和白细胞计数联合预测严重创伤合并脓毒症的效能评价

目的:评价肝素结合蛋白(HBP)联合脏器功能指标对严重创伤合并脓毒症患者预警诊断和预后预测效能评价研究。方法:回顾性分析2019年1月~2020年9月期间入住浙江大学医学院附属第二医院急诊医学科的多发伤并完成HBP检测患者184例,根据SEPSIS 3.0诊断标准将患者分为脓毒症组(n=89)和非脓毒症组(n=95),追踪患者临床结局分为死亡组(n=43)和非死亡组(n=141)。连续测定患者HBP水平,比较两组HBP峰值差异,评估其诊断脓毒症的效力,以HBP峰值的中位数为界值进一步分析其与临床预后相关性,评估HBP单独及联合总胆红素(TBil)及白细胞(WBC)评估预后的效力。结果:(1)脓毒症组(n=89)与非脓毒症组(n=95)HBP的峰值(71.7±68.6 vs 52.5±56.1)无显著差异(P=0.051)。(2)184例患者中HBP峰值与WBC计数呈正相关(r=0.244,P<0.01),与TBil水平呈正相关(r=0.241,P<0.01)。(3)TBil水平、WBC计数及PCT水平独立诊断脓毒症曲线下面积(AUC)分别是:0.618、0.631和0.718,三者联合AUC为0.684,诊断敏感度为60.7%,特异度为71.6%(P<0.05)。(4)死亡预后相关分析显示:高HBP水平组患者死亡率要显著高于低水平组(30.4%vs 16.3%,P<0.05);WBC计数值也是死亡组显著高于非死亡组(17.5±6.9 vs 12.8±4.7,P<0.01),尤其合并脓毒症者,该值有显著差异(P<0.01)。HBP峰值、TBil水平、WBC计数、SOFA评分及APACHE-II评分对预测脓毒症死亡预后的AUC分别是:0.618、0.603、0.719、0.823及0.811,HBP联合TBil及WBC评估脓毒症预后的AUC为0.750,评估的敏感度为74.4%,特异度为74.5%(P<0.05)。(5)三者联合评估在预测脓毒症预后效力上与人工评分差异无统计学意义(P>0.05)。结论:HBP、TBil及WBC三者联合用于评估多发伤患者发生脓毒症风险的预测效力较高,对于合并脓毒症的外伤患者死亡风险预测具有较高的临床指导价值。

血清Hcy、TBIL联合SUA检测对首发缺血性脑卒中及不良预后的预测价值

目的探讨血清同型半胱氨酸(Hcy)、总胆红素(TBIL)联合血尿酸(SUA)水平检测对首发缺血性脑卒中(FIS)及不良预后的预测价值。方法该研究为回顾性分析,选择2020年1月至2021年7月在该院神经内科住院治疗的210例FIS患者作为观察组,另匹配同期体检健康者210例作为对照组。比较两组血清Hcy、TBIL和SUA水平。统计FIS患者出院后1个月时的脑卒中致残、复发情况并使用改良Rankin量表(mRS)评估出院3个月时的预后情况,采用多因素Logistic回归分析FIS及其不良预后事件的危险因素。采用受试者工作特征(ROC)曲线评价血清Hcy、TBIL和SUA联合检测对FIS及复发、致残、mRS评分≥3分等不良预后事件的预测价值。结果与对照组相比,FIS患者的血清Hcy和SUA水平均显著升高,血清TBIL水平显著降低,差异有统计学意义(P<0.01)。出现复发、致残和mRS评分≥3分等不良预后事件的患者群体血清Hcy和SUA水平显著升高,血清TBIL水平显著降低,差异有统计学意义(P<0.01)。多因素Logistic回归分析结果显示,血清Hcy、TBIL和SUA不仅是FIS的影响因素(P<0.01),也是FIS患者疾病复发、致残和mRS评分≥3分等不良预后事件的影响因素(P<0.01)。ROC曲线分析结果显示,血清Hcy、TBIL和SUA 3项指标联合诊断FIS的曲线下面积(AUC)为0.835(95%CI:0.789~0.875),3项指标联合诊断FIS的效能显著优于单指标检测(P<0.01)。3项指标联合检测对致残(AUC=0.859,95%CI:0.804~0.903)、复发(AUC=0.807,95%CI:0.747~0.858)、mRS≥3分(AUC=0.847,95%CI:0.791~0.893)等不良预后事件也具备一定的预测效能,且预测效能显著优于单指标检测(P<0.05)。结论血清Hcy、TBIL和SUA水平与FIS发生及预后密切相关,血清Hcy、TBIL和SUA联合检测在FIS的诊断和预后评估中具有较高的临床价值。

胆红素总量/白蛋白比值、听力筛查及MRI-T_(1)WI影像对新生儿急性胆红素脑病的诊断价值

目的:探究胆红素总量/白蛋白比值(B/A)、听力筛查及磁共振成像-T_(1)加权成像(MRI-T_(1)WI)对新生儿急性胆红素脑病(ABE)的诊断价值。方法:选取2013年6月—2023年6月于太仓市第一人民医院接受治疗的136例新生儿高胆红素血症(NHB)患儿作为研究对象,根据是否并发ABE分为并发组(n=42)和对照组(n=94)。对比两组的临床资料、B/A水平、听力筛查结果、苍白球T_(1)WI信号强度,对单因素分析有意义的指标进行多因素logistic回归分析,采用ROC曲线分析其对新生儿ABE的诊断价值。结果:并发组总血清胆红素(TSB)峰值、B/A水平均高于对照组,差异有统计学意义(P<0.05)。并发组初筛听力异常率为38.10%,复筛听力异常率为35.71%,均高于对照组的18.09%、8.51%,差异有统计学意义(P<0.05)。并发组苍白球与额叶脑皮质T_(1)WI信号强度比值(G/Gr)水平高于对照组,差异有统计学意义(P<0.05)。logistic回归分析显示,B/A、听力异常和G/Gr水平表达均为影响NHB患儿并发ABE的独立危险因素,差异有统计学意义(P<0.05)。ROC曲线分析显示,B/A、G/Gr、听力异常及三者联合检测NHB患儿并发ABE的AUC值分别为0.773、0.652、0.754、0.863,有较高诊断价值,且联合诊断价值最高(P<0.05)。结论:ABE患儿中B/A、听力异常和G/Gr表达水平明显升高,三者单独和联合检测NHB患儿并发ABE均有较高诊断价值,其中三者联合诊断效果最佳。

云南地区53例先天性高胆红素血症临床特征及UGT1A1基因多态性分析

目的探讨云南地区53例UGT1A1基因突变所致的先天性高胆红素血症(Gilbert综合征、Crigler-Najjar综合征)的临床特征及UGT1A1基因突变特点。方法选取2017年1月至2022年12月间昆明市儿童医院消化内科53例Gilbert综合征(GS组)、Crigler-NajjarⅡ型综合征(CN-2组)患儿的临床数据、UGT1A1基因突变结果,回顾性分析患儿的临床特征、实验室检查结果和基因多态性。结果少数民族患儿均在2组中发病率较高,CN-2组中女性比例比GS高。肝脏系统方面,CN-2组TBil、IDB水平较GS组更高,差异均有统计学意义(P<0.05),GS组肝脏结构正常,CN-2组有3例患者肝胆结构有异常。肝外系统方面,血液系统、糖代谢无异常,血脂、甲状腺功能代谢有异常,GS组有维生素D不足,CN-2组存在维生素A、D缺乏及维生素E水平下降。2组患儿均存在心脏结构异常,但CN-2组较GS组发病率高。在所有患者中,突变频率最高的为发生在5号外显子上的c.1456T>G(32例,60.37%),其次为c.1091C>T(14例,26.42%)、1号外显子c.211G>A(6例,11.32%)和c.1198A>C(4例,7.55%)。c.1456T>G位点突变在GS组和CN-2组的频率分别为69.23%和57.5%(9例和23例),2组间差异无统计学意义(P>0.05)。2组中,其次突变频率较高的为c.1091C>T(4例和10例),2组间差异无统计学意义(P>0.05)。UGT1A1基因单倍型1、3、4在GS组和CN-2组间的频率差异均有统计学意义(P<0.05)。UGT1A1基因的4种主要突变形式,在性别和不同民族间,差异无统计学意义(P>0.05)。结论GS组和CN-2组在肝脏系统、肝外系统的临床表现存在不同,UGT1A1基因突变频率最高的为发生在5号外显子上的c.1456T>G。

DBIL/TBIL表达与超声引导下射频消融治疗原发性肝癌患者复发的关系

目的分析血清直接胆红素(DBIL)/总胆红素(TBIL)比值与超声引导下射频消融治疗原发性肝癌(PLC)患者复发的关系。方法入选2018年3月至2021年2月124例拟行射频消融治疗的PLC患者作为研究对象,根据研究目的,在射频消融治疗前常规检测相关指标,包括血清DBIL、TBIL、肿瘤标志物及患者一般临床资料。以射频消融治疗达到完全消融视为消融成功,将消融治疗成功后2 a内复发视为随访终点,采用Cox回归,分析血清DBIL/TBIL与超声引导下射频消融治疗PLC患者复发的关系。结果2018年3月至2021年2月共收录124例PLC患者,一次消融成功率为92.06%(116/126),复发率为21.55%(25/116),射频消融治疗后复发患者肿瘤数目、肿瘤直径大于未复发患者,血清TBIL、DBIL低于未复发患者,甲胎蛋白、DBIL/TBIL比值高于未复发患者(P<0.05)。构建Cox回归分析显示,肿瘤数量≥3、瘤体直径、血清TBIL表达下调,DBIL/TBIL比值升高导致PLC患者射频消融治疗后复发风险增加(P<0.05)。绘制受试者工作特征曲线,血清DBIL/TBIL比值预测PLC患者射频消融治疗后复发的曲线下面积为0.776,当DBIL/TBIL截断值达到0.50时,可以获得最高敏感度(0.920)和特异度(0.495)。结论血清DBIL/TBIL比值升高导致PLC患者射频消融治疗后2 a内复发风险增加,消融治疗前计算DBIL/TBIL比值有助于预测复发风险。

T2DM患者不同亚型血清胆红素水平与糖尿病肾病的相关性研究

目的探讨2型糖尿病(type 2 diabetes mellitus,T2DM)患者不同亚型血清胆红素水平与糖尿病肾病(diabetic nephropathy,DN)的相关性。方法回顾性分析湖北医药学院附属随州市中心医院2019年1月至2023年5月住院的494例T2DM患者的临床资料,按是否为DN将其分为DN组(175例)和非DN组(319例)。分析血清总胆红素(total bilirubin,TBil)、直接胆红素(direct bilirubin,DBil)、间接胆红素(indirect bilirubin,IBil)水平与DN发生的相关性。结果两组患者的年龄、糖尿病病程、高血压、冠心病、吸烟、饮酒、TBil、DBil、IBil、丙氨酸转氨酶(alanine aminotransferase,ALT)、血尿素氮(blood urea nitrogen,BUN)、血肌酐(serum creatinine,SCr)、尿酸(uric acid,UA)、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、低密度脂蛋白(low density lipoprotein,LDL)、高密度脂蛋白(high density lipoprotein,HDL)、糖化血红蛋白(glycosylated hemoglobin,HbA1c)、体质量指数(body mass index,BMI)、收缩压、空腹血糖(fasting plasma glucose,FPG)、估算的肾小球滤过率比较差异均有统计学意义(P<0.05)。年龄、糖尿病病程、高血压、冠心病、TBil、DBil、IBil、ALT、BUN、SCr、UA、TC、TG、HDL、LDL、HbA1c、BMI和FPG是女性T2DM患者发生DN的影响因素(P<0.05),年龄、糖尿病病程、高血压、冠心病、吸烟、饮酒、TBil、DBil、IBil、ALT、BUN、SCr、UA、TC、TG、HDL、LDL、HbA1c、BMI和FPG是男性T2DM患者发生DN的影响因素(P<0.05)。T2DM患者中,TBil、DBil、IBil与DN发生显著相关(P<0.001),随着TBil(1~15μmol/L)、IBil(1~10μmol/L)、DBil(1~4μmol/L)范围内升高,DN发生风险逐渐降低,但在TBil>15μmol/L、IBil>10μmol/L、DBil>4μmol/L后,TBil、DBil、IBil水平增高不再降低DN发病风险。结论T2DM患者的TBil、DBil、IBil水平均与DN患病风险存在非线性关联,一定水平范围内TBil、DBil、IBil是DN发生的独立保护因素,达到一定水平不再降低DN的发生风险;不同性别下不同亚型血清胆红素与DN的患病风险无明显差异。

新生儿病理性黄疸血清总胆红素、AST、ALT、ALB水平与蓝光治疗效果的关系

目的 研究新生儿病理性黄疸血清总胆红素、谷草转氨酶(AST)、谷丙转氨酶(ALT)、白蛋白(ALB)水平与蓝光治疗效果的关系。方法 收集2020年6月至2022年12月期间于南阳市中心医院新生儿重症监护病区诊治的97例新生儿病理性黄疸的病历资料,根据患儿蓝光照射治疗效果评估结果分为有效组和无效组,其中有效组共57例,无效组共40例。两组患儿均接受蓝光照射治疗,并进行血清总胆红素、AST、ALT、ALB检测。比较两组治疗前黄疸指数、血清总胆红素、AST、ALT、ALB水平,并分析患儿治疗前血清总胆红素、AST、ALT、ALB水平与蓝光治疗效果相关性。结果 治疗前,有效组黄疸指数低于无效组,差异有统计学意义(t=3.346,P<0.05);有效组血清总胆红素、AST、ALT水平低于无效组,血清ALB水平高于无效组,差异有统计学意义(t=3.635、2.273、2.167、8.689,P<0.05);经Logistic多因素分析,总胆红素、AST、ALT水平变化均为影响新生儿病理性黄疸病情严重程度的独立危险因素,ALB水平变化为影响新生儿病理性黄疸病情严重程度的独立保护因素(P<0.05);经Spearman相关性分析显示,患儿治疗前血清总胆红素、AST、ALT与病情严重程度成正比,ALB水平与病情严重程度成反比(P<0.05)。结论 治疗前新生儿病理性黄疸血清总胆红素、AST、ALT水平越低,ALB越高,可提示患儿病情程度越轻,从而越有利于蓝光治疗效果。

胰管支架置入对急性胆源性胰腺炎患者预后及并发症的影响

目的分析急性胆源性胰腺炎(ABP)患者应用胰管支架置入治疗对预后及并发症的影响。方法选取2019年1月至2022年12月收治的300例ABP患者,按治疗方法不同分组,A组100例行鼻胆管引流治疗,B组100例行开腹胆总管探查联合T型管引流术治疗,C组100例行鼻胆管引流联合胰管支架置入治疗,对比3组肝功能、并发症、死亡率及恢复情况。结果B组术后总胆红素(TBIL)、天冬酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)水平低于A组(P<0.05);C组术后TBIL、AST、ALT水平低于A组和B组(P<0.05);B组恢复进食时间、体温恢复时间、住院时间较A组更短(P<0.05);C组恢复进食时间、腹痛消失时间、体温恢复时间及住院时间短于A组和B组(P<0.05);C组并发症发生率4.00%低于A组的12.00%(P<0.05);C组1.00%死亡率低于A组8.00%(P<0.05)。结论ABP患者应用胰管支架置入治疗,可有缩短患者恢复时间,有利于改善肝功能,死亡率低,且并发症少。

新生儿病理性黄疸血清总胆红素、甲状腺功能、转铁蛋白表达水平及其临床意义

目的探究新生儿病理性黄疸血清总胆红素(TBIL)、转铁蛋白(TRF)、甲状腺功能指标[甲状腺素(T_(4))、三碘甲状腺原氨酸(T_(3))、促甲状腺素(TSH)]的表达水平及临床意义。方法回顾性分析150例朝阳妇幼保健院和华北石油管理局总医院2022年1月至2023年12月期间收治的病理性黄疸新生儿资料,根据血清TBIL水平分为轻度组、中度组和重度组;所有患儿均接受蓝光照射治疗,根据治疗效果将其分为有效组(112例)和无效组(38例),收集患儿基本资料,比较不同病情、不同治疗效果患儿血清TBIL、TRF、T_(4)、T_(3)、TSH表达水平差异;Pearson相关性分析血清TBIL、TRF、T_(4)、T_(3)、TSH与病理性黄疸患儿病情严重程度的关系;多因素Logistic回归分析患儿治疗无效的影响因素。结果随新生儿病理性黄疸病情加重,其血清TBIL水平逐渐升高,TRF、T_(3)逐渐下降,差异有统计学意义(F=495.073、110.290、19.609,均P<0.05);重度组患儿血清T_(4)水平较轻度组显著下降,差异有统计学意义(t=2.747,P<0.05);TRF、T_(3)水平均与TBIL呈负相关关系(r=-0.745、-0.683,P<0.05);蓝光照射治疗无效组患儿血清TBIL水平高于有效组,TRF、T_(3)水平则低于有效组,差异有统计学意义(t=4.698、17.174、5.796,均P<0.05),多因素Logistic回归分析显示血清TBIL、TRF、T_(3)均是患儿治疗无效的独立影响因素(P<0.05)。结论新生儿病理性黄疸血清TBIL、TRF、T_(3)水平与患儿病情严重程度有关,TBIL水平升高,TRF、T_(3)水平降低,可影响蓝光治疗效果,有助于新生儿病理性黄疸的诊疗评估。

不明原因缺血性脑卒中潜在危险因素的分析研究

目的分析不明原因缺血性脑卒中的潜在危险因素。方法选取2017年6月至2022年6月江西省抚州市第一人民医院神经内科50例不明原因缺血性脑卒中住院患者作为试验组,并选取同一时期非缺血性脑卒中及既往无脑卒中病史的50例住院患者作为对照组。采用单因素及多因素分析两组患者的一般资料及血液指标与不明原因缺血性脑卒中的关系。结果单因素结果显示,试验组中的年龄、收缩压、体重指数(BMI)、尿酸(UA)、肌酐(SCr)、平均血小板体积(MPV)、大血小板比率(P-LCR)、红细胞体积分布宽度(RDW)均高于对照组,而总胆红素(TBil)低于对照组,差异有统计学意义(P<0.05)。多因素分析结果显示,高UA(β=0.005,OR=1.005,95%CI=1.001~1.009)、高SCr(β=0.021,OR=1.021,95%CI=1.005~1.038)、高MPV(β=1.818,OR=6.158,95%CI=2.892~13.113)、高P-LCR(β=0.685,OR=1.983,95%CI=1.459~2.694)、高RDW(β=0.343,OR=1.410,95%CI=1.165~1.705)是不明原因缺血性脑卒中的独立危险因素(P<0.05);高TBil(β=-0.375,OR=0.687,95%CI=0.590~0.802)是不明原因缺血性脑卒中的独立保护因素(P<0.05)。结论UA、SCr、MPV、P-LCR、RDW以及TBil是不明原因缺血性脑卒中的独立影响因素,其中高UA、SCr、MPV、P-LCR、RDW是不明原因缺血性脑卒中的独立危险因素,高TBil是不明原因缺血性脑卒中的独立保护因素。

总胆红素改良候选参考方法与常规检测系统检测结果的可比性分析

目的:依据CLSI EP9-A3的要求评价两种检测系统检测血清总胆红素(T-BIL)结果的可比性,以评估两种检测系统检测结果的偏倚与一致性。方法:收集2021年8月北京航天总医院门诊及住院患者完成检测后的剩余血清40份,以德国汉诺威临床化学研究所改良T-BIL候选参考方法作为参比系统,实验室在用的常规检测系统作为待评系统,首先对两个系统进行正确度实验,再对两组系统的检测结果进行统计学分析,选取Passing-Baklok回归模型拟合回归方程,计算其在医学决定水平处的偏移,以国家卫健委临床检验中心室间质评1/2TEa(7.5%)为可接受标准进行结果评价。结果:待评系统与参比系统正确度实验结果显示:两个系统的检测结果的均在标物规定有效范围之内。使用Passing-Baklok回归对结果进行线性拟合,回归方程为Y=0.973X-1.397(r=0.998)。将血清T-BIL医学决定水平值34.20、171.00、342.00分别代入回归方程,计算得到的相对偏移分别为-7.02%、-3.58%、-3.16%,小于国家卫健委临床检验中心室间质评1/2TEa(7.5%),满足要求。结论:实验室在用的常规检测系统与德国汉诺威临床化学研究所T-BIL改良候选参考方法间存在一定负偏移,在医学决定水平处的偏移可接受,满足临床需要,改良T-BIL候选参考方法应用于常规检测系统检测结果评价时效果良好。

基于总胆红素和白蛋白比值的新生儿急性胆红素脑病预测模型

目的构建和验证结合总胆红素和白蛋白比值(B/A)的预测模型,以预测高胆红素血症新生儿发生急性胆红素脑病(ABE)的风险。方法选取2015年1月至2022年12月期间在黄河三门峡医院住院的535例高胆红素血症新生儿为研究对象,根据有无ABE的发生,将患儿分为ABE新生儿(n=96)和无ABE新生儿(n=439)两组。比较两组新生儿人口统计学特征、病理信息及入院检查结果等,受试者工作特征(ROC)曲线分析各变量预测ABE发生的能力。单因素和多因素Logistic回归分析评估急性胆红素脑病的影响因素,并进一步构建列线图模型。采用ROC曲线、校正曲线评估模型的效能,采用临床决策曲线(DCA)评估模型的临床实用价值。结果多因素Logistic回归分析显示,母龄、白细胞计数、总胆红素、B/A、高敏C反应蛋白(CRP)是新生儿胆红素脑病的独立危险因素(aOR值分别为2.548、3.640、1.236、6.289、1.680,P<0.05),白蛋白是新生儿胆红素脑病的独立保护因素(aOR=0.456,P<0.05);进一步构建了基于母亲年龄、白细胞计数、B/A、CRP共4个变量的ABE发生风险的列线图,模型C指数为0.879,曲线下面积(AUC)为0.899(95%CI:0.869~0.930),且校准曲线显示模型具有较好的辨别能力;DCA显示模型在实际临床工作中使得患者有更多获益。结论B/A是高胆红素血症新生儿发生ABE的独立影响因素和有效预测指标,构建的包含B/A的列线图,具有良好预测能力。

网织红细胞及血清TBiL联合溶血三项在新生儿溶血病中的临床诊断分析

目的探讨网织红细胞及血清总胆红素(TBi L)联合溶血三项在新生儿溶血病中的临床诊断价值。方法对安阳市中医院2019年10月至2022年10月收治的140例疑似新生儿溶血病的新生儿患者的临床资料进行回顾性分析,以临床确诊为标准,将所有患儿分为非新生儿溶血病组(40例)和新生儿溶血病组(100例)。检测两组患儿溶血三项、网织红细胞及血清TBi L。分析溶血三项的检测结果,对比溶血三项检查与临床确诊新生儿溶血病的结果,将新生儿溶血病组和非新生儿溶血病组患儿网织红细胞、血清TBi L水平进行对比,分析溶血三项、网织红细胞、血清TBi L,以及上述指标联合检测诊断新生儿溶血病的临床价值。结果140例疑似新生儿溶血病的新生儿患者中确诊患儿100例,非疾病患儿40例,溶血三项检测结果诊断出新生儿溶血病患儿102例,阳性检出率为72.86%,非新生儿溶血病患儿38例(27.14%),新生儿溶血病组患儿网织红细胞(6.99±1.40)%、TBi L(237.21±77.11)μmol/L均比非新生儿溶血病组高(3.47±0.95)%、(170.05±48.65)μmol/L(均P<0.05);溶血三项诊断新生儿溶血病的灵敏度为89.00%(89/100),特异度为67.50%(27/40),阳性预测值和阴性预测值分别为87.25%(89/102)、71.05%(27/38);以5.11%、198.75μmol/L为网织红细胞、TBi L诊断新生儿溶血病的最佳截断值,溶血三项、网织红细胞、血清TBi L及联合检测诊断新生儿溶血病的灵敏度、特异度分别为95.70%、87.56%,均比单项检测的数值高。结论新生儿溶血病患儿网织红细胞、TBi L均比非新生儿溶血病组高,且溶血三项、网织红细胞及血清TBi L联合检测应用于对新生儿溶血病的诊断中,临床应用价值较高。

直接胆红素、总胆红素、炎性因子在腹腔镜胆囊切除术围手术期的变化及术后并发症风险预测模型构建

目的:探讨直接胆红素、总胆红素(TBIL)及炎性因子在腹腔镜胆囊切除术(LC)围手术期的变化,并以此构建LC术后并发症风险预测模型。方法:选择2022年1月至2023年5月收治的296例LC患者,收集患者临床资料,分析手术前后血清直接胆红素、TBIL及炎性因子水平,根据患者术后1个月内有无并发症发生分为并发症组与无并发症组,进行单因素分析与多因素Logistic回归模型分析,构建风险预测模型。结果:并发症组手术前后各时点血清直接胆红素、TBIL、白细胞介素-6、肿瘤坏死因子-α、C反应蛋白均高于无并发症组(P<0.05),年龄、体质指数、胆囊壁厚度及胆囊与周围脏器粘连、Calot三角粘连、胆囊颈部结石、糖尿病史的比例亦高于无并发症组(P<0.05)。Logistic回归结果显示,年龄、糖尿病史、胆囊壁厚度、胆囊与周围脏器粘连、术前血清TBIL、白细胞介素-6、肿瘤坏因子-α、C反应蛋白水平均是LC术后出现并发症的独立危险因素(P<0.05)。受试者工作特征曲线分析显示,曲线下面积为0.756(95%CI=0.658~0.825,P<0.05)。结论:术前TBIL、白细胞介素-6、肿瘤坏死因子-α、C反应蛋白水平升高均是LC术后发生并发症的独立危险因素,以此构建的预测模型对胆囊疾病患者LC术后发生并发症的预测价值较高。