BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding ...BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding region, so its polymorphism may result in the abnormality of APOE gene and protein expression, and finally lead to dementia. OBJECTIVE: To observe the association between APOE promoter-219G/T polymorphisms with serum total cholesterol in patients with Alzheimer disease, and compare it with non-dementia people. DESIGN: Case-control, comparative observation. SETTING: Department of Neurology, Fengtian Hospital of Shenyang Medical College. PARTICIPANTS: Fifty-five dementia patients including 27 males and 28 females aged (66±3) years and treated in the Department of Neurology, Fengtian Hospital were selected from January 2002 to December 2005 as the Alzheimer disease group. They all diagnosed according to the DSM-Ⅳdiagnostic criteria of Alzheimer disease instituted by American Psychiatry Association in 1994. Meanwhile, 44 none-dementia patients including 21 males and 23 females aged (66±3) years were selected from other clinical departments of Fengtian Hospital as control group. All the participants were informed the detection and agreed. METHODS: Genomic DNA was extracted from the peripheral blood of all subjects, then 'NEST'PCR, DNA sequence and enzyme digestion were adopted to detect the expression of APOE promoter-219 polymorphism, following by biomedical statistics analysis based on the clinical total cholesterol level. MAIN OUTCOME MEASURES: Polymorphism of APOE promoter-219 G/T and total cholesterol level. RESULTS: All 55 dementia patients and 44 non-dementia ones were involved in the result analysis. ①Allele and genotype frequency: The T allele frequency of the Alzheimer disease group was significantly higher than that in the control group [88.2% (97/110), 54.5% (48/88)], while G allele frequency was remarkably lower than that in the control group [11.8%(13/110), 45.5%(40/88), χ2=8.2, P < 0.01]. The TT allele frequency of the Alzheimer disease group was significantly higher than that in the control group [76% (42/55), 48% (21/44)], while GT+GG allele frequency was remarkably lower than that in the control group [24%(13/55), 52%(23/44), χ2=8.7, P < 0.01]. ②Total cholesterol level: The level of the TT genotype patients in the Alzheimer group was obviously higher than that in GT+GG genotype patients (t =2.46, P < 0.05); the cholesterol level in the two genotypes of the control group was similar (P > 0.05). CONCLUSION: TT genotype and allele T in the APOE promoter-219 polymorphisms are the sensitive gene, and genotype TT has a relationship with the increase of total cholesterol level.展开更多
BACKGROUND In recent years,the incidence of colorectal cancer(CRC)has been increasing.With the popularization of endoscopic technology,a number of early CRC has been diagnosed.However,despite current treatment methods...BACKGROUND In recent years,the incidence of colorectal cancer(CRC)has been increasing.With the popularization of endoscopic technology,a number of early CRC has been diagnosed.However,despite current treatment methods,some patients with early CRC still experience postoperative recurrence and metastasis.AIM To search for indicators associated with early CRC recurrence and metastasis to identify high-risk populations.METHODS A total of 513 patients with pT2N0M0 or pT3N0M0 CRC were retrospectively enrolled in this study.Results of blood routine test,liver and kidney function tests and tumor markers were collected before surgery.Patients were followed up through disease-specific database and telephone interviews.Tumor recurrence,metastasis or death were used as the end point of study to find the risk factors and predictive value related to early CRC recurrence and metastasis.RESULTS We comprehensively compared the predictive value of preoperative blood routine,blood biochemistry and tumor markers for disease-free survival(DFS)and overall survival(OS)of CRC.Cox multivariate analysis demonstrated that low platelet count was significantly associated with poor DFS[hazard ratio(HR)=0.995,95% confidence interval(CI):0.991-0.999,P=0.015],while serum carcinoembryonic antigen(CEA)level(HR=1.008,95%CI:1.001-1.016,P=0.027)and serum total cholesterol level(HR=1.538,95%CI:1.026-2.305,P=0.037)were independent risk factors for OS.The cutoff value of serum CEA level for predicting OS was 2.74 ng/mL.Although the OS of CRC patients with serum CEA higher than the cutoff value was worse than those with lower CEA level,the difference between the two groups was not statistically significant(P=0.075).CONCLUSION For patients with T2N0M0 or T3N0M0 CRC,preoperative platelet count was a protective factor for DFS,while serum CEA level was an independent risk factor for OS.Given that these measures are easier to detect and more acceptable to patients,they may have broader applications.展开更多
目的分析甲状腺结节发生与糖脂代谢水平的相关性。方法选取厦门弘爱医院(2022年1—12月)接受甲状腺检查者100例,根据患者的检查结果不同分组,其中检查结果为甲状腺结节的患者50例为研究组,检查结果为未发生甲状腺结节的患者50例为对照...目的分析甲状腺结节发生与糖脂代谢水平的相关性。方法选取厦门弘爱医院(2022年1—12月)接受甲状腺检查者100例,根据患者的检查结果不同分组,其中检查结果为甲状腺结节的患者50例为研究组,检查结果为未发生甲状腺结节的患者50例为对照组。分析患者的基础资料,明确甲状腺结节发生与糖脂代谢水平[空腹血糖(fasting plasma glucose,FPG)、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)及高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)]的相关性。结果研究组FPG、TC、TG和LDL-C水平较对照组更高,差异有统计学意义(P<0.05)。而在HDL-C指标相比之下,差异无统计学意义(P>0.05)。因FT3、FT4、TSH均有不确定值为等级资料行Spearman秩相关分析。TSH与FPG、TC、LDL-C、HDL-C均呈正相关(r值分别为0.105、0.635、0.143、0.704、0.619,P<0.01),而FT3与FPG、TC、LDL-C、HDL-C均呈负相关(r值分别为-0.568、-0.676、-0.352、-0.702、-0.764,P<0.01),FT4与FPG、TC、LDL-C、HDL-C均呈负相关(r值分别为-0.401、-0.547、-0.425、-0.639、-0.493,P<0.01)。结论血清FPG、TC、TG、LDL-C升高,HDL-C降低,为甲状腺结节诱发因素。展开更多
目的:观察益气养阴活血汤对冠状动脉粥样硬化性心脏病介入治疗术后患者血脂水平的影响。方法:选取2014年2月至2016年4月本院收治入院的行介入治疗的冠状动脉粥样硬化性心脏病患者80例,随机分为对照组和治疗组,每组各40例。对照组给予瑞...目的:观察益气养阴活血汤对冠状动脉粥样硬化性心脏病介入治疗术后患者血脂水平的影响。方法:选取2014年2月至2016年4月本院收治入院的行介入治疗的冠状动脉粥样硬化性心脏病患者80例,随机分为对照组和治疗组,每组各40例。对照组给予瑞舒伐他汀,治疗组在对照组的基础上联合益气养阴活血汤。观察两组患者治疗前后血脂水平[三酰甘油(three acyl glycerol,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein,HDL)及低密度脂蛋白(low density lipoprotein,LDL)]变化、心脏缺血事件发生情况及不良反应情况。结果:两组患者治疗后TC及LDL-C水平低于治疗前,且治疗组低于对照组,差异具有统计学意义(P<0.05)。对照组心脏缺血发生率为45.0%,治疗组心脏缺血发生率为10.0%,两组患者心脏缺血发生率比较,差异具有统计学意义(P<0.05)。对照组不良反应发生率为5.0%,治疗组不良反应发生率为27.5%,两组患者不良反应发生率比较,差异具有统计学意义(P<0.05)。结论:益气养阴活血汤运用于冠状动脉粥样硬化性心脏病介入治疗后,能够有效改善患者TC、LDL-C水平,还可有效避免心脏缺血及不良反应发生率。展开更多
文摘BACKGROUND: Many researches have suggested that apolipoprotein E (APOE) and total cholesterol metabolism are closely related with dementia. In the supposed theory, 219 site of APOE promoter region is near gene coding region, so its polymorphism may result in the abnormality of APOE gene and protein expression, and finally lead to dementia. OBJECTIVE: To observe the association between APOE promoter-219G/T polymorphisms with serum total cholesterol in patients with Alzheimer disease, and compare it with non-dementia people. DESIGN: Case-control, comparative observation. SETTING: Department of Neurology, Fengtian Hospital of Shenyang Medical College. PARTICIPANTS: Fifty-five dementia patients including 27 males and 28 females aged (66±3) years and treated in the Department of Neurology, Fengtian Hospital were selected from January 2002 to December 2005 as the Alzheimer disease group. They all diagnosed according to the DSM-Ⅳdiagnostic criteria of Alzheimer disease instituted by American Psychiatry Association in 1994. Meanwhile, 44 none-dementia patients including 21 males and 23 females aged (66±3) years were selected from other clinical departments of Fengtian Hospital as control group. All the participants were informed the detection and agreed. METHODS: Genomic DNA was extracted from the peripheral blood of all subjects, then 'NEST'PCR, DNA sequence and enzyme digestion were adopted to detect the expression of APOE promoter-219 polymorphism, following by biomedical statistics analysis based on the clinical total cholesterol level. MAIN OUTCOME MEASURES: Polymorphism of APOE promoter-219 G/T and total cholesterol level. RESULTS: All 55 dementia patients and 44 non-dementia ones were involved in the result analysis. ①Allele and genotype frequency: The T allele frequency of the Alzheimer disease group was significantly higher than that in the control group [88.2% (97/110), 54.5% (48/88)], while G allele frequency was remarkably lower than that in the control group [11.8%(13/110), 45.5%(40/88), χ2=8.2, P < 0.01]. The TT allele frequency of the Alzheimer disease group was significantly higher than that in the control group [76% (42/55), 48% (21/44)], while GT+GG allele frequency was remarkably lower than that in the control group [24%(13/55), 52%(23/44), χ2=8.7, P < 0.01]. ②Total cholesterol level: The level of the TT genotype patients in the Alzheimer group was obviously higher than that in GT+GG genotype patients (t =2.46, P < 0.05); the cholesterol level in the two genotypes of the control group was similar (P > 0.05). CONCLUSION: TT genotype and allele T in the APOE promoter-219 polymorphisms are the sensitive gene, and genotype TT has a relationship with the increase of total cholesterol level.
文摘BACKGROUND In recent years,the incidence of colorectal cancer(CRC)has been increasing.With the popularization of endoscopic technology,a number of early CRC has been diagnosed.However,despite current treatment methods,some patients with early CRC still experience postoperative recurrence and metastasis.AIM To search for indicators associated with early CRC recurrence and metastasis to identify high-risk populations.METHODS A total of 513 patients with pT2N0M0 or pT3N0M0 CRC were retrospectively enrolled in this study.Results of blood routine test,liver and kidney function tests and tumor markers were collected before surgery.Patients were followed up through disease-specific database and telephone interviews.Tumor recurrence,metastasis or death were used as the end point of study to find the risk factors and predictive value related to early CRC recurrence and metastasis.RESULTS We comprehensively compared the predictive value of preoperative blood routine,blood biochemistry and tumor markers for disease-free survival(DFS)and overall survival(OS)of CRC.Cox multivariate analysis demonstrated that low platelet count was significantly associated with poor DFS[hazard ratio(HR)=0.995,95% confidence interval(CI):0.991-0.999,P=0.015],while serum carcinoembryonic antigen(CEA)level(HR=1.008,95%CI:1.001-1.016,P=0.027)and serum total cholesterol level(HR=1.538,95%CI:1.026-2.305,P=0.037)were independent risk factors for OS.The cutoff value of serum CEA level for predicting OS was 2.74 ng/mL.Although the OS of CRC patients with serum CEA higher than the cutoff value was worse than those with lower CEA level,the difference between the two groups was not statistically significant(P=0.075).CONCLUSION For patients with T2N0M0 or T3N0M0 CRC,preoperative platelet count was a protective factor for DFS,while serum CEA level was an independent risk factor for OS.Given that these measures are easier to detect and more acceptable to patients,they may have broader applications.
文摘目的分析甲状腺结节发生与糖脂代谢水平的相关性。方法选取厦门弘爱医院(2022年1—12月)接受甲状腺检查者100例,根据患者的检查结果不同分组,其中检查结果为甲状腺结节的患者50例为研究组,检查结果为未发生甲状腺结节的患者50例为对照组。分析患者的基础资料,明确甲状腺结节发生与糖脂代谢水平[空腹血糖(fasting plasma glucose,FPG)、总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)及高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)]的相关性。结果研究组FPG、TC、TG和LDL-C水平较对照组更高,差异有统计学意义(P<0.05)。而在HDL-C指标相比之下,差异无统计学意义(P>0.05)。因FT3、FT4、TSH均有不确定值为等级资料行Spearman秩相关分析。TSH与FPG、TC、LDL-C、HDL-C均呈正相关(r值分别为0.105、0.635、0.143、0.704、0.619,P<0.01),而FT3与FPG、TC、LDL-C、HDL-C均呈负相关(r值分别为-0.568、-0.676、-0.352、-0.702、-0.764,P<0.01),FT4与FPG、TC、LDL-C、HDL-C均呈负相关(r值分别为-0.401、-0.547、-0.425、-0.639、-0.493,P<0.01)。结论血清FPG、TC、TG、LDL-C升高,HDL-C降低,为甲状腺结节诱发因素。
文摘目的:观察益气养阴活血汤对冠状动脉粥样硬化性心脏病介入治疗术后患者血脂水平的影响。方法:选取2014年2月至2016年4月本院收治入院的行介入治疗的冠状动脉粥样硬化性心脏病患者80例,随机分为对照组和治疗组,每组各40例。对照组给予瑞舒伐他汀,治疗组在对照组的基础上联合益气养阴活血汤。观察两组患者治疗前后血脂水平[三酰甘油(three acyl glycerol,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein,HDL)及低密度脂蛋白(low density lipoprotein,LDL)]变化、心脏缺血事件发生情况及不良反应情况。结果:两组患者治疗后TC及LDL-C水平低于治疗前,且治疗组低于对照组,差异具有统计学意义(P<0.05)。对照组心脏缺血发生率为45.0%,治疗组心脏缺血发生率为10.0%,两组患者心脏缺血发生率比较,差异具有统计学意义(P<0.05)。对照组不良反应发生率为5.0%,治疗组不良反应发生率为27.5%,两组患者不良反应发生率比较,差异具有统计学意义(P<0.05)。结论:益气养阴活血汤运用于冠状动脉粥样硬化性心脏病介入治疗后,能够有效改善患者TC、LDL-C水平,还可有效避免心脏缺血及不良反应发生率。
