Objective:To investigate the effect of total ginsenoside of ginseng stems and leaves (TGSL) on the pharmacokinetics of aspirin in rats.Methods:Sprague-Dawley rats were randomly divided into two groups (n =6),a combine...Objective:To investigate the effect of total ginsenoside of ginseng stems and leaves (TGSL) on the pharmacokinetics of aspirin in rats.Methods:Sprague-Dawley rats were randomly divided into two groups (n =6),a combined group that received TGSL (625 mg/kg body weight) and aspirin (10 mg/kg body weight) by gavage,and an aspirin group that received aspirin (10 mg/kg body weight) by gavage.The concentration of salicylic acid,an important metabolite of aspirin,was determined by highperformance liquid chromatography in supernatant from blood obtained from the orbital sinus at various time points to examine the effect of TGSL on aspirin.Results:The results showed that the Tmax of salicylic acid was [0.92 (0.58)] hours in the aspirin group and [2.50 (1.22)] hours in the combined group,and was statistically significantly different between the groups (p <.05).Conclusions:TGSL can affect the pharmacokinetics of aspirin at Tmax in rats.展开更多
A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2...A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2 2. On the basis of chemical and physicochemical evidence , the structure of ginsenoside-Rg6 have been elucidated as 6-O-(-L-rhamnosyl-(1?2)-(-D-glucopyranosyl-dammarane-(E)-20(22), 24-diene-3(, 6(, 12(-triol.展开更多
Shenshao Tablet(SST),prepared from Paeoniae Radix Alba(PRA)and total ginsenoside of Ginseng Stems and Leaves(GSL),is a traditional Chinese medicine(TCM)preparation prescribed to treat coronary heart disease.However,it...Shenshao Tablet(SST),prepared from Paeoniae Radix Alba(PRA)and total ginsenoside of Ginseng Stems and Leaves(GSL),is a traditional Chinese medicine(TCM)preparation prescribed to treat coronary heart disease.However,its chemical composition and the components that can migrate into blood potentially exerting the therapeutic effects have rarely been elucidated.We developed an HPLC/DAD/ESI-MS^n approach aiming to comprehensively profile and identify both the chemical components of SST and its absorbed ingredients(and metabolites)in rat plasma and urine.Chromatographic separation was performed on an Agilent Eclipse XDB C_(18) column using acetonitrile/0.1% formic acid as the mobile phase.MS detection was conducted in both negative and positive ESI modes to yield more structure information.Comparison with reference compounds(t_R,MS^n),interpretation of the fragmentation pathways,and searching of in-house database,were utilized for more reliable structure elucidation.A total of 82 components,including 21monoterpene glycosides,four galloyl glucoses,two phenols from PRA,and 55 ginsenosides from GSL,were identified or tentatively characterized from the 70% ethanolic extract of SST.Amongst them,seven and 24 prototype compounds could be detectable in the plasma and urine samples,respectively,after oral administration of an SST extract(4 g×kg^(–1))in rats.No metabolites were observed in the rat samples.The findings of this work first unveiled the chemical complexity of SST and its absorbed components,which would be beneficial to understanding the therapeutic basis and quality control of SST.展开更多
文摘Objective:To investigate the effect of total ginsenoside of ginseng stems and leaves (TGSL) on the pharmacokinetics of aspirin in rats.Methods:Sprague-Dawley rats were randomly divided into two groups (n =6),a combined group that received TGSL (625 mg/kg body weight) and aspirin (10 mg/kg body weight) by gavage,and an aspirin group that received aspirin (10 mg/kg body weight) by gavage.The concentration of salicylic acid,an important metabolite of aspirin,was determined by highperformance liquid chromatography in supernatant from blood obtained from the orbital sinus at various time points to examine the effect of TGSL on aspirin.Results:The results showed that the Tmax of salicylic acid was [0.92 (0.58)] hours in the aspirin group and [2.50 (1.22)] hours in the combined group,and was statistically significantly different between the groups (p <.05).Conclusions:TGSL can affect the pharmacokinetics of aspirin at Tmax in rats.
基金The Ninth 5-year Plan" Key Science and Technique R & D Programme Foundation of China (96-901-01-12A).
文摘A novel dammarane-type triterpene oligoglycoside, named ginsenoside-Rg6 3, was isolated from the stem-leaves of Panax ginseng C. A. Mey., together with two known ones, 20(S)-ginsenoside-Rg2 1 and 20(R)-ginsenoside-Rg2 2. On the basis of chemical and physicochemical evidence , the structure of ginsenoside-Rg6 have been elucidated as 6-O-(-L-rhamnosyl-(1?2)-(-D-glucopyranosyl-dammarane-(E)-20(22), 24-diene-3(, 6(, 12(-triol.
基金financially supported by Tianjin Municipal Education Commission Research Project(No.2017ZD07)
文摘Shenshao Tablet(SST),prepared from Paeoniae Radix Alba(PRA)and total ginsenoside of Ginseng Stems and Leaves(GSL),is a traditional Chinese medicine(TCM)preparation prescribed to treat coronary heart disease.However,its chemical composition and the components that can migrate into blood potentially exerting the therapeutic effects have rarely been elucidated.We developed an HPLC/DAD/ESI-MS^n approach aiming to comprehensively profile and identify both the chemical components of SST and its absorbed ingredients(and metabolites)in rat plasma and urine.Chromatographic separation was performed on an Agilent Eclipse XDB C_(18) column using acetonitrile/0.1% formic acid as the mobile phase.MS detection was conducted in both negative and positive ESI modes to yield more structure information.Comparison with reference compounds(t_R,MS^n),interpretation of the fragmentation pathways,and searching of in-house database,were utilized for more reliable structure elucidation.A total of 82 components,including 21monoterpene glycosides,four galloyl glucoses,two phenols from PRA,and 55 ginsenosides from GSL,were identified or tentatively characterized from the 70% ethanolic extract of SST.Amongst them,seven and 24 prototype compounds could be detectable in the plasma and urine samples,respectively,after oral administration of an SST extract(4 g×kg^(–1))in rats.No metabolites were observed in the rat samples.The findings of this work first unveiled the chemical complexity of SST and its absorbed components,which would be beneficial to understanding the therapeutic basis and quality control of SST.
