We reported an unusual case of disseminated cryptococcal lymphadenitis in an immunocompetent host who presented with fever and lymphadenopathy, which were the only two symptoms and signs. Latex agglutination test of s...We reported an unusual case of disseminated cryptococcal lymphadenitis in an immunocompetent host who presented with fever and lymphadenopathy, which were the only two symptoms and signs. Latex agglutination test of serum and cerebrospinal fluid （CSF） were negative, while lymph node biopsy showed Cryptococcus neoformans. A diagnosis of disseminated cryptococcal lymphadenitis was made. Then the patient was treated with amphotericin B for 15 days as initial therapy and itraconazole for 6 months as maintenance therapy respectively. The patient received re-examination per 6 months and was followed up for 2 years. Swollen lymph nodes diminished gradually, and no fever or other symptoms were found. Latex agglutination test of serum and CSF were negative throughout the follow-up period, and anti-HIV, syphilis and tuberculosis antibody were all negative.展开更多
The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine repr...The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.展开更多
In order to develop a simple and safe test for the detection of vaccinated as well as wild type Pseudorabies virus (PRV) infected pigs,the modified gE gene of PRV Ea strain,obtained by cutting the 5’ UTR using PCR an...In order to develop a simple and safe test for the detection of vaccinated as well as wild type Pseudorabies virus (PRV) infected pigs,the modified gE gene of PRV Ea strain,obtained by cutting the 5’ UTR using PCR and DNA recombinant technique,was inserted into baculovirus expression vector pFastBac 1,resulting the trans-position plamid pFE1.75.After homologous recombination,recombinant baculovirus rvBacE1.75 was gained and high level expression of glycoprotein E (gE) was observed after the infection of rvBacE1.75 to Tn-5B1-4 cells.The expression product was 80～88kD and was specific to antisera against PRV Ea strain by Western-blotting.Purified recombinant proteins were used as an antigen in Latex Agglutination Test(gE-LAT) and the test was specific,sensitive,safe and simple.展开更多
文摘We reported an unusual case of disseminated cryptococcal lymphadenitis in an immunocompetent host who presented with fever and lymphadenopathy, which were the only two symptoms and signs. Latex agglutination test of serum and cerebrospinal fluid （CSF） were negative, while lymph node biopsy showed Cryptococcus neoformans. A diagnosis of disseminated cryptococcal lymphadenitis was made. Then the patient was treated with amphotericin B for 15 days as initial therapy and itraconazole for 6 months as maintenance therapy respectively. The patient received re-examination per 6 months and was followed up for 2 years. Swollen lymph nodes diminished gradually, and no fever or other symptoms were found. Latex agglutination test of serum and CSF were negative throughout the follow-up period, and anti-HIV, syphilis and tuberculosis antibody were all negative.
文摘The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration,temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.
文摘In order to develop a simple and safe test for the detection of vaccinated as well as wild type Pseudorabies virus (PRV) infected pigs,the modified gE gene of PRV Ea strain,obtained by cutting the 5’ UTR using PCR and DNA recombinant technique,was inserted into baculovirus expression vector pFastBac 1,resulting the trans-position plamid pFE1.75.After homologous recombination,recombinant baculovirus rvBacE1.75 was gained and high level expression of glycoprotein E (gE) was observed after the infection of rvBacE1.75 to Tn-5B1-4 cells.The expression product was 80～88kD and was specific to antisera against PRV Ea strain by Western-blotting.Purified recombinant proteins were used as an antigen in Latex Agglutination Test(gE-LAT) and the test was specific,sensitive,safe and simple.
