The influence of intestine-based treatment using Xuan Bai Cheng Qi Tang on the concentration of trace elements in the main organs of COPD rats

Objective:To test two theories from traditional Chinese medicine:'exterior -interior relationship between the lung and large intestine' and 'treating from the intestine principle for lung disorders'.The influence of intestine-based treatment using Xuan Bai Cheng Qi Tang (XBCQT) on the concentration of three trace elements-copper (Cu),zinc (Zn),and manganese (Mn)-was observed in the tissues of the lung,small intestine,large intestine,and stomach of rats suffering from chronic obstructive pulmonary disease (COPD).Methods:Thirty-five male Wistar rats were divided randomly and equally into five groups:control;model;Fei treatment (A);Chang treatment (B);and Fei-Chang treatment (C).A rat model of COPD was prepared by tracheal injection of lipopolysaccharide plus exposure to cigarette smoke.Treatments with medicinal herbs started day-22 of administration and exposure to cigarette smoke for 7 days.The control group and model group were administered physiologic (0.9%) saline solution via the stomach.After 7 days of intervention,the tissues of the lung,small intestine,large intestine,and stomach were removed.Inductively coupled plasma-atomic emission spectroscopy was used to detect the levels of Cu,Zn,and Mn in those tissues.Results:Compared with the control group,the Cu concentration in the tissues of the small intestine,large intestine,and stomach increased significantly in the model group (P <.05);the Mn concentration in the tissues of the lung,large intestine,and stomach increased significantly in the model group (P <.05);the Zn concentration in the tissues of the lung and large intestine decreased significantly in the model group (P <.05).In comparison of the model group:the Cu concentration in the tissues of the lung and large intestine decreased significantly in the B group (P <.05);the Mn concentration in the tissues of the lung,small intestine,and large intestine decreased significantly in the B group (P <.05);the Zn concentration in the tissues of the lung,small intestine,and large intestine increased significantly in the B group (P <.05).For the A group versus C group comparison,the Zn concentration in the tissues of the small intestine and stomach increased significantly in the latter (P <.05).Conclusion:This study showed that 'treating from the intestine' using Xuan Bai Cheng Qi Tang and its modified formulae can regulate the concentration of trace elements in the main organs of COPD rats.This may be one of the mechanisms for intestine-based treatment for COPD.

大肠癌组织和癌旁组织微量元素分析

[目的]探讨微量元素在大肠癌组织和癌旁组织中的含量及其变化规律。[方法]采用电感耦合等离子体发射光谱法和原子荧光法对35例结肠癌患者癌及癌旁组织中Mn、Fe、Cu、Zn、Mo、Se等6种微量元素含量进行测定。[结果]结肠癌组织中Se的含量明显低于癌旁(P﹤0.05),Fe、Cu、Mo、等含量降低,Mn和Zn含量升高,但无统计学意义。[结论]大肠癌组织和癌旁组织中元素的含量有差异,说明微量元素与癌症的发生、发展有密切的关系。

大肠癌与微量元素研究

对４８例大肠癌患者手术前后血清和正常大肠组织与癌组织中微量元素测定、比较。发现大肠癌患者手术前后血清微量元素变化，除Ｃｒ、Ｍｎ有显著差异外均无显著差异，而癌瘤组织与正常大肠组织中Ｃａ、Ｃｄ、Ｍｎ、Ｚｎ、Ｃｕ／Ｚｎ有明显差异。探讨了大肠癌发生的机理．大肠癌病因是复杂的，但与微量元素改变有一定关系。并指出，组织测定观察微量元素变化比血清测定观察更敏感。

大肠癌与溃疡性结肠炎患者血清微量元素测定

用ＩＣＰ－ＡＥＳ方法，对３０例大肠癌、２６例溃疡性结肠炎进行血清钼、锌、铬、铁、铜、锰、铅、铝、硒、铋、钡、钛、钴、钒等微量元素测定，并与２０例健康者对照。结果显示大肠癌组血清钼（±ＳＤ＝０．２１±０．１０μｍｏｌ／Ｌ）、锌（±ＳＤ＝１．６５±１．３４μｍｏｌ／Ｌ）、铬（±ＳＤ＝１．６５±１．３４μｍｏｌ／Ｌ）低于对照组钼（±ＳＤ＝１．１９±０．３１μｍｏｌ／Ｌ）、锌（±ＳＤ＝２０．９４±６．３１μｍｏｌ／Ｌ）、铬（±ＳＤ＝２．８８±１．１５μｍｏｌ／Ｌ），Ｐ分别＜０．０１、＜０．０５、＜０．０５；溃疡性结肠炎组钼（±ＳＤ＝０．２１±０．０８μｍｏｌ／Ｌ）低于对照组，Ｐ＜０．０５。提示微量元素代谢异常可能直接影响酶系统，使自由基失控，体内促氧化和抗氧化平衡失调，促使肿瘤的发生。钼、锌、铬在大肠癌的发病中起一定作用。

微量元素检测与临床应用研究

研究显示,随食管上皮病变的进展,血清铜呈显著增加趋势;大肠癌患者血清锌水平明显低于正常对照和大肠良性病组;糖尿病患者血清锌,铁明显低于对照组,而血清铜则高于对照组,经t检验差别有显著意义。本文就微量元素多种相关疾病中的应用简要概述。

不同发病情况大肠癌的微量元素变化

分析了近１０年内本院手术切除的大肠癌标本３０１例，从中抽取３１例用原子吸收分光光度计检测癌组织与远离癌的肠粘膜中Ｃｕ、Ｚｎ、Ｆｅ、Ｍｎ、Ｎｉ等微量元素含量，发现病程长者Ｚｎ、Ｎｉ含量降低，隆起缩窄型者锌含量低于溃疡型，癌肿位于结肠者Ｆｅ含量较位于直肠者为低。