Polymeric immunoglobulin receptors（pIgR） are key participants in the formation and secretion of secretory Ig A（S-Ig A）, which is critical for the prevention of microbial infection and colonization in the respirato...Polymeric immunoglobulin receptors（pIgR） are key participants in the formation and secretion of secretory Ig A（S-Ig A）, which is critical for the prevention of microbial infection and colonization in the respiratory system. Although increased respiratory colonization and infections are common in HIV/AIDS, little is known about the expression of pIgR in the airway mucosa of these patients. To address this, the expression levels of pIgR in the tracheal mucosa and lungs of SHIV/SIV-infected rhesus macaques were examined by real-time RTPCR and confocal microscopy. We found that the levels of both PIGR m RNA and pIgR immunoreactivity were lower in the tracheal mucosa of SHIV/SIVinfected rhesus macaques than that in non-infected rhesus macaques, and the difference in pIgR immunoreactivity was statistically significant. IL-17 A, which enhances pIgR expression, was also changed in the same direction as that of pIgR. In contrast to changes in the tracheal mucosa, pIgR and IL-17 A levels were higher in the lungs of infected rhesus macaques. These results indicated abnormal pIgR expression in SHIV/SIV, and by extension HIV infections, which might partially result from IL-17 A alterations and might contribute to the increased microbial colonization and infection related to pulmonary complications in HIV/AIDS.展开更多
目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8...目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8、24及48 h 5个时间点处死大鼠各3只。取气管下1/3段的腹侧部,采用扫描电镜观察气道表面结构。结果阿米卡星组均出现不同程度的黏液分泌增加,黏稠度增加,纤毛倒伏、粘着在一起、排列紊乱、部分断裂缺失,恢复较慢,2、4、8、24及48 h受损面积百分比分别为98.2%、98.5%、97.5%、92.7%及82.1%。氨溴索干预组上述改变较轻,修复较快,2、4、8、24及48 h受损面积百分比分别为85.7%、81.9%、73.0%、61.9%及50.2%,恢复明显较对照组快。经气管注药在各时间点阿米卡星组纤毛受损面积明显高于氨溴索干预组(P<0.01)。结论阿米卡星可对大鼠气道黏膜表面结构造成严重损害,氨溴索可促进气管黏膜的修复,氨溴索注射液法治疗气管损伤效果理想,值得临床推广使用。展开更多
基金supported by the Beijing Natural Science Foundation(7162136)
文摘Polymeric immunoglobulin receptors（pIgR） are key participants in the formation and secretion of secretory Ig A（S-Ig A）, which is critical for the prevention of microbial infection and colonization in the respiratory system. Although increased respiratory colonization and infections are common in HIV/AIDS, little is known about the expression of pIgR in the airway mucosa of these patients. To address this, the expression levels of pIgR in the tracheal mucosa and lungs of SHIV/SIV-infected rhesus macaques were examined by real-time RTPCR and confocal microscopy. We found that the levels of both PIGR m RNA and pIgR immunoreactivity were lower in the tracheal mucosa of SHIV/SIVinfected rhesus macaques than that in non-infected rhesus macaques, and the difference in pIgR immunoreactivity was statistically significant. IL-17 A, which enhances pIgR expression, was also changed in the same direction as that of pIgR. In contrast to changes in the tracheal mucosa, pIgR and IL-17 A levels were higher in the lungs of infected rhesus macaques. These results indicated abnormal pIgR expression in SHIV/SIV, and by extension HIV infections, which might partially result from IL-17 A alterations and might contribute to the increased microbial colonization and infection related to pulmonary complications in HIV/AIDS.
文摘目的观察腹腔注射盐酸氨溴索对气管内注射阿米卡星后大鼠气管黏膜损伤的超微结构的影响。方法将30只Wistar大鼠经气管穿刺注入阿米卡星,随机分为对照组(阿米卡星组,15只)及氨溴索干预组(15只),经大鼠腹腔注射盐酸氨溴索注射液,在2、4、8、24及48 h 5个时间点处死大鼠各3只。取气管下1/3段的腹侧部,采用扫描电镜观察气道表面结构。结果阿米卡星组均出现不同程度的黏液分泌增加,黏稠度增加,纤毛倒伏、粘着在一起、排列紊乱、部分断裂缺失,恢复较慢,2、4、8、24及48 h受损面积百分比分别为98.2%、98.5%、97.5%、92.7%及82.1%。氨溴索干预组上述改变较轻,修复较快,2、4、8、24及48 h受损面积百分比分别为85.7%、81.9%、73.0%、61.9%及50.2%,恢复明显较对照组快。经气管注药在各时间点阿米卡星组纤毛受损面积明显高于氨溴索干预组(P<0.01)。结论阿米卡星可对大鼠气道黏膜表面结构造成严重损害,氨溴索可促进气管黏膜的修复,氨溴索注射液法治疗气管损伤效果理想,值得临床推广使用。
