Observation of Traditional Chinese Medicine's Anti-bacterial Effect on the Main Pathogenic Bacteria of Dairy Cow Recessive Mastitis

[Objective]This paper aimed to study the anti-bacterial effect of traditional Chinese medicine on cow recessive mastitis＇ s main pathogenic bacteria and provide basis for its clinical application.[Method]Plating and test-tube methods were used to determine the anti-bacterial diameter,minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of 20 kinds of traditional Chinese medicine on dairy cow recessive mastitis＇ s clinically isolated main pathogenic bacteria,Staphylococcus aureus and Streptococcus agalactiae.[Conclusion]The results indicated that 7 kinds of traditional Chinese medicine,Herba Taraxaci,Rhizoma Coptidis,Fructus Forsythiae,Herba Andrographis,Radix Scutellar iae,Flos Carthami,Flos Chrysanthemi Indici had strong anti-bacterial effect.

中药感舒体内抗菌实验研究

目的通过感舒的体内抗菌实验，观察其抗菌作用，为其治疗上呼吸道感染性疾病提供实验依据。方法金黄色葡萄球菌和肺炎链球菌分别培养18h增殖后，用5％干酵母溶液稀释成不同浓度，分别对小鼠体内腹腔注射，选用80％～100％致死率的金黄色葡萄球菌和肺炎链球菌的5％干酵母溶液，感染给药小鼠，观测给药小鼠在染菌后7d内的平均存活天数和死亡率。结果中药感舒的1．00，0．50，0．25g/kg剂量均能不同程度的抑制金黄色葡萄球菌和肺炎链球菌感染小鼠的死亡率并延长平均存活天数。结论中药感舒具有一定的体内抗菌作用，与临床治疗上呼吸道感染效果相符。

24种中药及复方制剂对奶牛隐性乳房炎主要致病菌的抑菌试验

选取蒲公英、黄芪等24味中药及组成的两个复方制剂进行浸液制备。分别利用单味中药和两个复方制剂对致奶牛乳房炎的大肠杆菌、金黄色葡萄球菌和链球菌等进行体外抑菌试验,测定各种中药的最低抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明蒲公英、复方制剂一、复方制剂二对奶牛乳房炎主要致病菌具有很好的抑菌和杀菌作用。

五种蒙药体外抑制牛乳中主要病原菌生长的效果

目的观察大黄、黄芪、肉桂、蒲公英、丁香等5种蒙药对牛乳腺炎金黄葡萄球菌和无乳链球菌的体外抑菌效果。方法分别将上述5种蒙药制成水煎剂,在体外对分离的致病菌进行抑菌试验。结果大黄对金黄葡萄球菌和无乳链球菌的抑菌效果较好,黄芩、肉桂的抑菌效果次之,蒲公英和丁香的抑菌效果一般。结论蒙药大黄、黄芪、肉桂、蒲公英、丁香对金黄葡萄球菌和无乳链球菌有抑菌作用。该实验为进一步筛选治疗牛乳腺炎的蒙药奠定了基础。