Before a breeder invests selection pressure on a trait of interest, it needs to be established whether that trait is actually heritable. Some traits may not have been measured widely in pedigreed populations, for exam...Before a breeder invests selection pressure on a trait of interest, it needs to be established whether that trait is actually heritable. Some traits may not have been measured widely in pedigreed populations, for example, a disease or deformity may become more prevalent than previously, but is still relatively rare. One approach to detect inheritance would be to screen a commercial population to obtain a sample of "affecteds" (the test group) and to also obtain a random control group. These indi- viduals are then genotyped with a set of genetic markers and the relationships between individuals within each group estimated. If the relatedness is higher in the test group than in the control group, this provides initial evidence for the trait being heritable. A power simulation shows that this approach is feasible with moderate resources.展开更多
Quantitative trait locus(QTL) mapping is frequently used to understand the genetic architecture of quantitative traits.Herein,we performed a genome scanfor QTL affecting the morphometric characters in eight full-sib f...Quantitative trait locus(QTL) mapping is frequently used to understand the genetic architecture of quantitative traits.Herein,we performed a genome scanfor QTL affecting the morphometric characters in eight full-sib families containing 522 individuals using different statistical methods(Sib-pair and half-sib model).A total of 194 QTLs were detected in 25 different regions on 10 linkage groups(LGs).Among them,37 QTLs on five LGs(eight,13,24,40 and 45) were significant(5%genome-wide level),while the remaining 40(1%chromosome-wide level) and 117(5%chromosome-wide level) indicated suggestive effect on those traits.Heritabilities for most morphometric traits were moderate to high,ranging from 0.21 to 0.66,with generally strong phenotypic and genetic correlations between the traits.A large number of QTLs for morphometric traits were co-located,consistent with their high correlations,and may reflect pleiotropic effect on the same genes.Biological pathways were mapped for possible candidate genes on QTL regions.One significantly enriched pathway was identified onLG45,which had a P-value of 0.04 and corresponded to the "regulation of actin cytoskeleton pathway".The results are expected to be useful in marker-assisted selection(MAS) and provide valuable information for the study of gene pathway for morphometric and growth traits of the common carp.展开更多
文摘Before a breeder invests selection pressure on a trait of interest, it needs to be established whether that trait is actually heritable. Some traits may not have been measured widely in pedigreed populations, for example, a disease or deformity may become more prevalent than previously, but is still relatively rare. One approach to detect inheritance would be to screen a commercial population to obtain a sample of "affecteds" (the test group) and to also obtain a random control group. These indi- viduals are then genotyped with a set of genetic markers and the relationships between individuals within each group estimated. If the relatedness is higher in the test group than in the control group, this provides initial evidence for the trait being heritable. A power simulation shows that this approach is feasible with moderate resources.
基金supported by the National Natural Science Foundation of China(31302174)the Central-Level Non-profit Scientific Research Institutes Special Funds(HSY201303)the China Ministry of Agriculture Recommend International Advanced Agricultural Science and Technology Plan(2016-X15)
文摘Quantitative trait locus(QTL) mapping is frequently used to understand the genetic architecture of quantitative traits.Herein,we performed a genome scanfor QTL affecting the morphometric characters in eight full-sib families containing 522 individuals using different statistical methods(Sib-pair and half-sib model).A total of 194 QTLs were detected in 25 different regions on 10 linkage groups(LGs).Among them,37 QTLs on five LGs(eight,13,24,40 and 45) were significant(5%genome-wide level),while the remaining 40(1%chromosome-wide level) and 117(5%chromosome-wide level) indicated suggestive effect on those traits.Heritabilities for most morphometric traits were moderate to high,ranging from 0.21 to 0.66,with generally strong phenotypic and genetic correlations between the traits.A large number of QTLs for morphometric traits were co-located,consistent with their high correlations,and may reflect pleiotropic effect on the same genes.Biological pathways were mapped for possible candidate genes on QTL regions.One significantly enriched pathway was identified onLG45,which had a P-value of 0.04 and corresponded to the "regulation of actin cytoskeleton pathway".The results are expected to be useful in marker-assisted selection(MAS) and provide valuable information for the study of gene pathway for morphometric and growth traits of the common carp.
