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侧耳菌与粗毛栓菌在麦草培养基中产生木质纤维素降解酶的研究 被引量:24
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作者 谢君 孙迅 +1 位作者 任路 张义正 《生物工程学报》 CAS CSCD 北大核心 2001年第5期575-578,共4页
Pleurotus sp2 and Trametes gallica were selected in this assay because of their high activities of lignocellulolytic enzymes and the enzyme peaks appeared at the early stage of liquid state fermentation.Solid state fe... Pleurotus sp2 and Trametes gallica were selected in this assay because of their high activities of lignocellulolytic enzymes and the enzyme peaks appeared at the early stage of liquid state fermentation.Solid state fermentation was also investigated for their abilities and behaviors of enzyme\|production.The capabilities and characteristics of the two strains in degrading biomass were studied.When \Pleurotus sp2\ was incubated in wheat straw powder containing the liquid medium of low\|nitrogen,no\|carbon and high inorganic salt,the activities of MnP and Lac reached the peaks on the tenth day,but the activities of hemicellulases reached the peak on the 40th day.\Pleurotus sp2\ caused 17.6 of biomass loss.When \T.gallica was incubated in wheat straw powder containing the liquid medium of hlig\|nitrogen,or low\|nitrogen,no\|carbon and high inorganic salt,the activities of MnP reached the peaks on the tenth day,the lac and hemicelluloses on the 40th day,and the lignin peroxidases reached the peaks on the 50th day,and it caused more than 64% of biomass loss.Among them the hemicellulose was degraded by 71.96%,and the cellulose 66.21%. T.gallica% was very capable of degrading lignin of wheat straw and caused 34.37 loss during 20 days,46.71 loss during 30 days and 70.14% loss during 60 days.It was interesting that \T.gallica \degraded lignin preferentially with respect to cellulose,which was very beneficial to biopulping of paper industry. 展开更多
关键词 侧耳菌 粗毛栓菌 木质纤维素降解酶 生物质降解 麦草培养基
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白腐菌液体和固体培养产生木质纤维素降解酶的比较研究 被引量:10
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作者 谢君 黄乾明 +2 位作者 冯蕾 徐宁 杨军 《菌物学报》 CAS CSCD 北大核心 2007年第2期266-272,共7页
侧耳sp2(Pleurotus sp.2)和粗毛栓菌(Trametes gallica)是产木质纤维素降解酶能力强,且产酶较快的菌株。对其在液体培养基、固体培养基中产生木质纤维素降解酶能力和行为进行了比较分析和研究。结果表明,Pleurotus sp.2在低氮高碳高... 侧耳sp2(Pleurotus sp.2)和粗毛栓菌(Trametes gallica)是产木质纤维素降解酶能力强,且产酶较快的菌株。对其在液体培养基、固体培养基中产生木质纤维素降解酶能力和行为进行了比较分析和研究。结果表明,Pleurotus sp.2在低氮高碳高无机盐培养基中的锰过氧化物酶(Manganese peroxidases, MnPs)、木质素过氧化物酶(Lignin peroxidases.LiPs)、漆酶(laccases,Lacs)和半纤维素酶(Hemicellulases, Hcels)的活性最高。当该菌株培养在含有低氮无碳高无机盐液体培养基的麦草粉中时,MnPs和Lacs的活性峰值均出现在10d,而Hcels的活性在40d时达到峰值。Trametes gallica在高氮低碳高无机盐培养基中的Lacs和LiPs的活性最高,在低氮高碳高无机盐培养基中的MnPs和Hcels的活性最高。当该菌株培养在含有高氮无碳高无机盐和低氮无碳高无机盐液体培养基的麦草粉中时,MnPs存10d、Lacs和Hcels在40d、LiPs存50d,分别达到峰值。Pleurotus sp.2和Trametes gallica在液体培养基中具有很强的木质纤维素降解酶产生能力且产酶速度较快,在固体培养基中具有很强的降解麦秸生物质能力,但这两株菌在液体和固体培养基中,产木质纤维素降解酶的能力和行为都有较大的差异,相关性小。 展开更多
关键词 侧耳菌 粗毛栓菌 木质纤维素降解酶活性
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三种白腐菌及其组合菌种木质素降解酶比较研究 被引量:10
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作者 段传人 朱丽平 姚月良 《菌物学报》 CAS CSCD 北大核心 2009年第4期577-583,共7页
朱红栓菌Trametes cinnabarina、糙皮侧耳Pleurotus ostreatus、黄孢原毛平革菌Phanerochaete chrysosporium是产生木质素降解酶能力强的菌株。对三种白腐菌及其组合菌种产生木质素降解酶能力和行为进行了比较分析和研究。结果表明,最... 朱红栓菌Trametes cinnabarina、糙皮侧耳Pleurotus ostreatus、黄孢原毛平革菌Phanerochaete chrysosporium是产生木质素降解酶能力强的菌株。对三种白腐菌及其组合菌种产生木质素降解酶能力和行为进行了比较分析和研究。结果表明,最佳培养方式为液体振荡培养;最佳培养基为酵母膏液体培养基。在产漆酶(laccases,lacs)方面,Pleurotus ostreatus和Phanerochaete chrysosporium的组合菌种的酶活最强,在第6天出现峰值,酶活达到450U/L;在产锰过氧化物酶(manganese peroxidases,mnps)方面,Trametes cinnabarina和Pleurotus ostreatus的组合菌种的酶活最强,在第10天出现峰值,酶活达到1050U/L;在产木质素过氧化物酶(lignin peroxidases,lips)方面,Trametes cinnabarina和Phanerochaete chrysosporium的组合菌种的酶活最强,在第8天出现产酶峰值,酶活达到2990U/L。筛选结果表明,组合菌种比单菌种产生的三种主要木质素降解酶的活性强,这为白腐菌高效产酶提供了一条新的途径,并为白腐菌研究领域的后续工作奠定基础。 展开更多
关键词 朱红栓菌 糙皮侧耳 黄孢原毛平革菌 酶活
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