Objective:To examine the effect of moxibustion on the wound healing process and its mechanism using a rat wound model.Methods:Sixty male Sprague-Dawley rats were randomly divided into a sham-treated group(n=30,wound s...Objective:To examine the effect of moxibustion on the wound healing process and its mechanism using a rat wound model.Methods:Sixty male Sprague-Dawley rats were randomly divided into a sham-treated group(n=30,wound surgery only)and a moxibustion group(n=30,wound treated with moxibustion).Circular fullthick ness skin wounds were produced in rats.Moxibustion was applied to the edge of wound and was continued on alternating days till 14 days after surgery,followed by measurement of wound size.Expression of collagens,prolyl-4-hydroxylase(P4H)and transtorming growth factor-β(TGF-β)were evaluated by histochemical study and real-time polymerase chain reaction.Results:The size of the wound lesion was significantly reduced in rats treated with moxibustion as compared to that in sham-treated rats at 4-10 days after wounding(P<0.01).Moxibusti on stimulated mRNA expressi on of collage ns at 4 days(P<0.01),but not at 7 days,accompa nied by enhanced proliferation of P4H-positive fibroblasts.Of importanee,expression of TGF-β in tissue from the wound lesio n treated with moxibusti on was sign ifica ntly in creased as compared to that in sham-treated rats at 4 days(P<0.01 or P<0.05),but not at 7 days.Conclusions:The treatment with moxibustion promoted the wound healing process in the early phase through proliferation of fibroblasts and rapid formation of granulation,possibly mediated by induction of TGF-β which is a key molecule in the physiological process of wound healing.Moxibustion can be expected to be effective as complementary treatment for intractable ulcers.展开更多
目的：研究肝素与大鼠肝星状细胞（hepatic stellate cells，HSC）作用后转化生长因子β1（transforming growth factor β1，TGF-β1）和I型胶原表达的变化及意义。方法：大鼠肝星状细胞以1×10^8／L浓度接种于96孔培养板，每孔10...目的：研究肝素与大鼠肝星状细胞（hepatic stellate cells，HSC）作用后转化生长因子β1（transforming growth factor β1，TGF-β1）和I型胶原表达的变化及意义。方法：大鼠肝星状细胞以1×10^8／L浓度接种于96孔培养板，每孔100uL。实验分组为肝素I组、肝素Ⅱ组、肝素Ⅲ组，加入肝素使各组培养液中肝素浓度分别是10，100，1000mg／L，加生理盐水为对照组（每组6孔重复3次）培养48h。培养终止后吸取上清液-20℃冰冻保存，ELISA法检测其上清液TGF-β1和I型胶原水平，MTT法观察细胞增殖情况。结果：肝素Ⅱ组和Ⅲ组HSC培养上清液TGF-β1水平均显著低于对照组（4.59±1.27ng／L，3.34±1.13ng／L VS 5.95±1.72ng／L，P〈0．01），肝素各组I型胶原水平均低于对照组（87．20±9．30ng／L．73．17±12．04ng/L VS 95．61±12．55ng／L，63．31±10．93ng／L VS 95．61±12．55ng／L，P均〈0．05），肝素Ⅲ组平均吸光度低于肝素I组和对照组（0．29±0．07 VS 0．42±0．12，0．46±0．17，P均〈0．05）。结论：大鼠肝星状细胞在肝素作用TTGF-β1和I型胶原分泌受抑制，其增殖减少。展开更多
文摘Objective:To examine the effect of moxibustion on the wound healing process and its mechanism using a rat wound model.Methods:Sixty male Sprague-Dawley rats were randomly divided into a sham-treated group(n=30,wound surgery only)and a moxibustion group(n=30,wound treated with moxibustion).Circular fullthick ness skin wounds were produced in rats.Moxibustion was applied to the edge of wound and was continued on alternating days till 14 days after surgery,followed by measurement of wound size.Expression of collagens,prolyl-4-hydroxylase(P4H)and transtorming growth factor-β(TGF-β)were evaluated by histochemical study and real-time polymerase chain reaction.Results:The size of the wound lesion was significantly reduced in rats treated with moxibustion as compared to that in sham-treated rats at 4-10 days after wounding(P<0.01).Moxibusti on stimulated mRNA expressi on of collage ns at 4 days(P<0.01),but not at 7 days,accompa nied by enhanced proliferation of P4H-positive fibroblasts.Of importanee,expression of TGF-β in tissue from the wound lesio n treated with moxibusti on was sign ifica ntly in creased as compared to that in sham-treated rats at 4 days(P<0.01 or P<0.05),but not at 7 days.Conclusions:The treatment with moxibustion promoted the wound healing process in the early phase through proliferation of fibroblasts and rapid formation of granulation,possibly mediated by induction of TGF-β which is a key molecule in the physiological process of wound healing.Moxibustion can be expected to be effective as complementary treatment for intractable ulcers.
文摘目的：研究肝素与大鼠肝星状细胞（hepatic stellate cells，HSC）作用后转化生长因子β1（transforming growth factor β1，TGF-β1）和I型胶原表达的变化及意义。方法：大鼠肝星状细胞以1×10^8／L浓度接种于96孔培养板，每孔100uL。实验分组为肝素I组、肝素Ⅱ组、肝素Ⅲ组，加入肝素使各组培养液中肝素浓度分别是10，100，1000mg／L，加生理盐水为对照组（每组6孔重复3次）培养48h。培养终止后吸取上清液-20℃冰冻保存，ELISA法检测其上清液TGF-β1和I型胶原水平，MTT法观察细胞增殖情况。结果：肝素Ⅱ组和Ⅲ组HSC培养上清液TGF-β1水平均显著低于对照组（4.59±1.27ng／L，3.34±1.13ng／L VS 5.95±1.72ng／L，P〈0．01），肝素各组I型胶原水平均低于对照组（87．20±9．30ng／L．73．17±12．04ng/L VS 95．61±12．55ng／L，63．31±10．93ng／L VS 95．61±12．55ng／L，P均〈0．05），肝素Ⅲ组平均吸光度低于肝素I组和对照组（0．29±0．07 VS 0．42±0．12，0．46±0．17，P均〈0．05）。结论：大鼠肝星状细胞在肝素作用TTGF-β1和I型胶原分泌受抑制，其增殖减少。
