Objective To evaluate the level of apoptosis in the corneal epithelium after photorefractive keratectomy(PRK).Methods Six rabbits were anesthetized systemically with sodium pentobarbital.PRK was then performed and the...Objective To evaluate the level of apoptosis in the corneal epithelium after photorefractive keratectomy(PRK).Methods Six rabbits were anesthetized systemically with sodium pentobarbital.PRK was then performed and the central 5.6mm area of the cornea was ablated to a depth of 156μm(-8.0D).Rabbits were killed 1 month and 3 months after the performance respectivelly,and the cornea were prepared for frozen tissue sections.The apoptosis of the corneal epithelium was evaluated by TUNEL method.Results The normal corneal epithelium exhibited a limited level of apoptosis,primarily in the superficial epithelium.The entire epithelial layer was found to be apoptotic 1 month after PRK.The apoptosis of the corneal epithelium 3 months after PRK was the same as the normal corneal epithelium.Conclusion The apoptosis of corneal epithelium exist in wound healing process after PRK.The findings suggest that apoptosis plays an important role in the corneal wound healing process.展开更多
AIM:To compare the efficacy between two different silicone hydrogel bandage contact lenses after transepithelial photorefractive keratectomy(T-PRK).METHODS:In this randomized controlled trial,a total of 89 patients(17...AIM:To compare the efficacy between two different silicone hydrogel bandage contact lenses after transepithelial photorefractive keratectomy(T-PRK).METHODS:In this randomized controlled trial,a total of 89 patients(178 eyes) who underwent T-PRK at the Qingdao Eye Hospital from October to December 2019 were selected.One random eye wore a Senofilcon A bandage contact lens after surgery,and the other eye a Balafilcon A bandage contact lens.Pain scores,uncorrected visual acuity(UCVA),spherical equivalent(SE),corneal epithelial healing status,epithelial thickness,bandage lenses deposits,lenses movement,and ocular surface conditions were measured and compared.RESULTS:There were no differences between the two groups in UCVA,SE,corneal epithelial healing status,corneal epithelial thickness,tear river heights and tear film rupture time at each follow-up visit.However,postoperative pain scores in the Senofilcon A group were significantly lower than those of the Balafilcon A group(F_(intergroups)=67.833,P<0.001;F_(time)=383.773,P<0.001;F_(interaction)=57.344,P<0.001).The duration of pain in eyes in the Senofilcon A group was shorter than that of the Balafilcon A group(t=-3.326,P=0.001).The surface deposition scores and movement scores of Senofilcon A bandage lenses on the first and fourth days after surgery were lower than those of Balafilcon A bandage lenses(Z=-5.385,-6.782,P<0.001;Z=-8.336,-8.906,P<0.001).CONCLUSION:Both Senofilcon A and Balafilcon A bandage lenses have good efficacy after T-PRK.Senofilcon A lenses are associated with less pain and more comfort compared to Balafilcon A.展开更多
文摘Objective To evaluate the level of apoptosis in the corneal epithelium after photorefractive keratectomy(PRK).Methods Six rabbits were anesthetized systemically with sodium pentobarbital.PRK was then performed and the central 5.6mm area of the cornea was ablated to a depth of 156μm(-8.0D).Rabbits were killed 1 month and 3 months after the performance respectivelly,and the cornea were prepared for frozen tissue sections.The apoptosis of the corneal epithelium was evaluated by TUNEL method.Results The normal corneal epithelium exhibited a limited level of apoptosis,primarily in the superficial epithelium.The entire epithelial layer was found to be apoptotic 1 month after PRK.The apoptosis of the corneal epithelium 3 months after PRK was the same as the normal corneal epithelium.Conclusion The apoptosis of corneal epithelium exist in wound healing process after PRK.The findings suggest that apoptosis plays an important role in the corneal wound healing process.
基金Supported by the Medical and Health Development Grant of Shandong Province,China (No.2017WS180)。
文摘AIM:To compare the efficacy between two different silicone hydrogel bandage contact lenses after transepithelial photorefractive keratectomy(T-PRK).METHODS:In this randomized controlled trial,a total of 89 patients(178 eyes) who underwent T-PRK at the Qingdao Eye Hospital from October to December 2019 were selected.One random eye wore a Senofilcon A bandage contact lens after surgery,and the other eye a Balafilcon A bandage contact lens.Pain scores,uncorrected visual acuity(UCVA),spherical equivalent(SE),corneal epithelial healing status,epithelial thickness,bandage lenses deposits,lenses movement,and ocular surface conditions were measured and compared.RESULTS:There were no differences between the two groups in UCVA,SE,corneal epithelial healing status,corneal epithelial thickness,tear river heights and tear film rupture time at each follow-up visit.However,postoperative pain scores in the Senofilcon A group were significantly lower than those of the Balafilcon A group(F_(intergroups)=67.833,P<0.001;F_(time)=383.773,P<0.001;F_(interaction)=57.344,P<0.001).The duration of pain in eyes in the Senofilcon A group was shorter than that of the Balafilcon A group(t=-3.326,P=0.001).The surface deposition scores and movement scores of Senofilcon A bandage lenses on the first and fourth days after surgery were lower than those of Balafilcon A bandage lenses(Z=-5.385,-6.782,P<0.001;Z=-8.336,-8.906,P<0.001).CONCLUSION:Both Senofilcon A and Balafilcon A bandage lenses have good efficacy after T-PRK.Senofilcon A lenses are associated with less pain and more comfort compared to Balafilcon A.
