Objective To study expression of adenoviral-mediated Hath1-EGFP gene in the guinea pig cochlea after transfer through intact round window membrane(RWM), and to assess its effects on hearing. Methods Twenty adult guine...Objective To study expression of adenoviral-mediated Hath1-EGFP gene in the guinea pig cochlea after transfer through intact round window membrane(RWM), and to assess its effects on hearing. Methods Twenty adult guinea pigs were used, of which: 12 were surgically inoculated with Ad-Hath1-EGFP in the bony groove of round window niche, and 8 with artificial perilymph. Auditory brainstem response(ABR) thresholds were determined in all animals before and 5 days after surgery. On post-surgery day 5 and day 14, animals were sacrificed and whole mounts of cochlea and frozen sections were examined. Results ABR tests showed no significant change of hearing after the surgery. Strong fluorescence staining in the cochleae was seen in Ad-Hath1-EGFP groups. The highest levels of gene expression were seen in the post-surgery day 5 group with little decrease on post-surgery day 14.The contralateral cochlea and those in the control groups were free of fluorescence staining. Conclusion The transgenic Hath1-EGFP can be effectively delivered into the inner ear through intact RWM, in an atraumatic manner.展开更多
Accurate prediction of thermal radiation by applying rigorous model for the radiative heat transfer combined with the conduction and the convection has been performed for a single and double window glazing subjected t...Accurate prediction of thermal radiation by applying rigorous model for the radiative heat transfer combined with the conduction and the convection has been performed for a single and double window glazing subjected to solar and thermal irradiation. The glass window is analysed as a non-gray plane-parallel medium disctritized to thin layer as-suming the glass material as participating media in one-dimensional case, using the Radiation Element Method by Ray Emission Model (REM2). The model allows the calculation of the steady-state heat flux and the temperature distribution within the glass cover. The spectral dependence of the relevant radiation properties of glass (i.e. specular reflectivity, refraction angle and absorption coefficient) is taken into account. Both solar and thermal incident irradiations are applied at the boundary surfaces using the spectral solar model proposed by Bird and Riordan. The optical constant of a commercial clear glass material have been used. The calculation has been performed during winter period and the effect of the thickness of the glass for a single glazing and of the air layer between the two panels for double glazing has been studied. The result shows that increasing the air layer, the steady heat flux decreases and the temperature distribution within the glass changes.展开更多
基金National NaturalScience Foundation grants No.30730040 and No.30628030.
文摘Objective To study expression of adenoviral-mediated Hath1-EGFP gene in the guinea pig cochlea after transfer through intact round window membrane(RWM), and to assess its effects on hearing. Methods Twenty adult guinea pigs were used, of which: 12 were surgically inoculated with Ad-Hath1-EGFP in the bony groove of round window niche, and 8 with artificial perilymph. Auditory brainstem response(ABR) thresholds were determined in all animals before and 5 days after surgery. On post-surgery day 5 and day 14, animals were sacrificed and whole mounts of cochlea and frozen sections were examined. Results ABR tests showed no significant change of hearing after the surgery. Strong fluorescence staining in the cochleae was seen in Ad-Hath1-EGFP groups. The highest levels of gene expression were seen in the post-surgery day 5 group with little decrease on post-surgery day 14.The contralateral cochlea and those in the control groups were free of fluorescence staining. Conclusion The transgenic Hath1-EGFP can be effectively delivered into the inner ear through intact RWM, in an atraumatic manner.
文摘Accurate prediction of thermal radiation by applying rigorous model for the radiative heat transfer combined with the conduction and the convection has been performed for a single and double window glazing subjected to solar and thermal irradiation. The glass window is analysed as a non-gray plane-parallel medium disctritized to thin layer as-suming the glass material as participating media in one-dimensional case, using the Radiation Element Method by Ray Emission Model (REM2). The model allows the calculation of the steady-state heat flux and the temperature distribution within the glass cover. The spectral dependence of the relevant radiation properties of glass (i.e. specular reflectivity, refraction angle and absorption coefficient) is taken into account. Both solar and thermal incident irradiations are applied at the boundary surfaces using the spectral solar model proposed by Bird and Riordan. The optical constant of a commercial clear glass material have been used. The calculation has been performed during winter period and the effect of the thickness of the glass for a single glazing and of the air layer between the two panels for double glazing has been studied. The result shows that increasing the air layer, the steady heat flux decreases and the temperature distribution within the glass changes.
