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Transglutaminase 2 serves as a pathogenic hub gene of KRAS mutant colon cancer based on integrated analysis
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作者 Wei-Bin Peng Yu-Ping Li +1 位作者 Yong Zeng Kai Chen 《World Journal of Gastrointestinal Oncology》 SCIE 2024年第5期2074-2090,共17页
BACKGROUND Colon cancer is acknowledged as one of the most common malignancies worldwide,ranking third in United States regarding incidence and mortality.Notably,approximately 40%of colon cancer cases harbor oncogenic... BACKGROUND Colon cancer is acknowledged as one of the most common malignancies worldwide,ranking third in United States regarding incidence and mortality.Notably,approximately 40%of colon cancer cases harbor oncogenic KRAS mutations,resulting in the continuous activation of epidermal growth factor receptor signaling.AIM To investigate the key pathogenic genes in KRAS mutant colon cancer holds considerable importance.METHODS Weighted gene co-expression network analysis,in combination with additional bioinformatics analysis,were conducted to screen the key factors driving the progression of KRAS mutant colon cancer.Meanwhile,various in vitro experiments were also conducted to explore the biological function of transglutaminase 2(TGM2).RESULTS Integrated analysis demonstrated that TGM2 acted as an independent prognostic factor for progression-free survival.Immunohistochemical analysis on tissue microarrays revealed that TGM2 was associated with an elevated probability of perineural invasion in patients with KRAS mutant colon cancer.Additionally,biological roles of the key gene TGM2 was also assessed,suggesting that the downregulation of TGM2 attenuated the proliferation,invasion,and migration of the KRAS mutant colon cancer cell line.CONCLUSION This study underscores the potential significance of TGM2 in the progression of KRAS mutant colon cancer.This insight not only offers a theoretical foundation for therapeutic approaches but also highlights the need for additional clinical trials and fundamental research to support our preliminary findings. 展开更多
关键词 Colon cancer KRAS mutation Transglutaminase 2 Weighted gene co-expression network analysis
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TG2基因修饰的鼻粘膜间充质干细胞向神经样细胞分化的研究 被引量:2
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作者 崔学文 史文涛 +5 位作者 戴瑶 毕士奇 杨开元 陈平波 孙勇 张志坚 《神经解剖学杂志》 CAS CSCD 北大核心 2018年第3期297-304,共8页
目的:研究重组transglutaminase 2(TG2)腺病毒转染对鼻粘膜骨髓间充质干细胞(EMSCs)向神经样细胞分化影响。方法:利用贴壁筛选法分离培养和扩增EMSCs,通过免疫荧光方法分析EMSCs的纯度,采用免疫荧光和免疫印迹方法测定转染效率。实验分... 目的:研究重组transglutaminase 2(TG2)腺病毒转染对鼻粘膜骨髓间充质干细胞(EMSCs)向神经样细胞分化影响。方法:利用贴壁筛选法分离培养和扩增EMSCs,通过免疫荧光方法分析EMSCs的纯度,采用免疫荧光和免疫印迹方法测定转染效率。实验分组:重组TG2腺病毒转染EMSCs(ad EMSCs)组、重组GFP腺病毒转染EMSCs组(GFP-EMSCs)和空白对照组(control),诱导细胞向神经样细胞分化,倒置显微镜观察分化过程中细胞形态的变化。免疫荧光和免疫印迹方法检测诱导后EMSCs的轴突膜蛋白(GAP-43),微管相关蛋白(MAP2)和髓磷脂碱性蛋白(MBP)的表达情况。向大鼠脊髓内注射ad EMSCs,2周后取脊髓。结果:实验数据表明转染TG2腺病毒的EMSCs可稳定表达TG2。诱导7 d后,ad EMSCs组细胞呈现双极和多极的典型神经样细胞形态,并且GAP-43,MAP2和MBP的表达水平较对照组高。移植入体内2周的ad EMSCs存活良好且部分细胞表达GAP-43。结论:TG2基因修饰有利于EMSCs在体外向神经样细胞分化,并且具有良好的可移植性。 展开更多
关键词 鼻粘膜 间充质干细胞 组织型谷胺酰氨转胺酶 神经样细胞
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Novel method for extracting exosomes of hepatocellular carcinoma cells 被引量:9
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作者 Lin Zhu Xiu-Hua Qu +2 位作者 Yu-Lin Sun Yang-Ming Qian Xiao-Hang Zhao 《World Journal of Gastroenterology》 SCIE CAS 2014年第21期6651-6657,共7页
AIM: To develop a novel method for the rapid and efficient extraction of exosomes secreted by tumor cells.
关键词 EXOSOME Membrane vesicles Tissue transglutaminase 2 Annexin A2 NANOMATERIALS
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特应性皮炎患者皮肤中转谷氨酰胺酶2蛋白的检测 被引量:2
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作者 沈辉 高艳薇 《实用皮肤病学杂志》 2018年第6期343-344,共2页
目的检测特应性皮炎患者皮肤中转谷氨酰胺酶2(TG2)蛋白表达的变化,探讨其在特应性皮炎致病中的作用。方法收集2017年1月—2018年1月我院皮肤科诊治的特应性皮炎患者52例的皮损组织,另取我院同期痣切除患者切缘周围正常皮肤标本30例作为... 目的检测特应性皮炎患者皮肤中转谷氨酰胺酶2(TG2)蛋白表达的变化,探讨其在特应性皮炎致病中的作用。方法收集2017年1月—2018年1月我院皮肤科诊治的特应性皮炎患者52例的皮损组织,另取我院同期痣切除患者切缘周围正常皮肤标本30例作为正常对照。酶联免疫吸附试验(ELISA)检测皮肤组织TG2蛋白表达水平。结果特应性皮炎患者皮损组织TG2蛋白为(11.57±3.25)ng/ml,显著高于对照组的(0.16±0.03)ng/ml(P <0.01)。特应性皮炎患者嗜酸粒细胞计数为(0.52±0.13)×10~9/L,显著高于对照组(0.31±0.07)×10~9/L(P<0.01)。特应性皮炎患者特应性皮炎积分(SCORAD)为(8.46±1.54)分,显著高于对照组(0.09±0.02)分(P <0.01)。TG2蛋白与特应性皮炎患者的嗜酸粒细胞计数(r=0.473,P <0.05)和SCORAD评分呈显著正相关(r=0.462,P <0.05)。结论特应性皮炎患者皮损组织中TG2蛋白高表达,TG2蛋白表达与患者的嗜酸粒细胞计数和临床SCORAD评分呈显著正相关。 展开更多
关键词 皮炎 特应性 转谷氨酰胺酶2
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Endomysial antibodies predict celiac disease irrespective of the titers or clinical presentation
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作者 Kalle Kurppa Tiia Rsnen +8 位作者 Pekka Collin Sari Iltanen Heini Huhtala Merja Ashorn Pivi Saavalainen Katri Haimila Jukka Partanen Markku Mki Katri Kaukinen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第20期2511-2516,共6页
AIM:To investigate the association between serum antibody levels and a subsequent celiac disease diag-nosis in a large series of children and adults. METHODS:Besides subjects with classical gastrointestinal presentati... AIM:To investigate the association between serum antibody levels and a subsequent celiac disease diag-nosis in a large series of children and adults. METHODS:Besides subjects with classical gastrointestinal presentation of celiac disease,the study cohort included a substantial number of individuals with extraintestinal symptoms and those found by screening in at-risk groups.Altogether 405 patients underwent clinical,serological and histological evaluations.After collection of data,the antibody values were further graded as low[endomysial(EmA)1:5-200,transglutaminase 2 antibodies(TG2-ab)5.0-30.0 U/L]and high (EmA 1:≥500,TG2-ab≥30.0 U/L),and the serological results were compared with the small intestinal mucosal histology and clinical presentation. RESULTS:In total,79%of the subjects with low and 94%of those with high serum EmA titers showed small-bowel mucosal villous atrophy.Furthermore, 96%of the 47 EmA positive subjects who had normal mucosal villi and remained on follow-up either subsequently developed mucosal atrophy while on a glutencontaining diet,or responded positively to a glutenfree diet. CONCLUSION:Irrespective of the initial serum titers or clinical presentation,EmA positivity as such is a very strong predictor of a subsequent celiac disease diagnosis. 展开更多
关键词 Celiac disease DIAGNOSIS Endomysial antibodies Transglutaminase 2 antibodies Clinical presentations
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