期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Transient receptor potential-related risk model predicts prognosis of hepatocellular carcinoma patients 被引量:1
1
作者 Xiao-Cai Mei Qian Chen Shi Zuo 《World Journal of Gastrointestinal Oncology》 SCIE 2023年第12期2064-2076,共13页
BACKGROUND Members of the transient receptor potential(TRP)protein family shape oncogenic development,but the specific relevance of TRP-related genes in hepatocellular carcinoma(HCC)has yet to be defined.AIM To invest... BACKGROUND Members of the transient receptor potential(TRP)protein family shape oncogenic development,but the specific relevance of TRP-related genes in hepatocellular carcinoma(HCC)has yet to be defined.AIM To investigate the role of TRP genes in HCC,their association with HCC development and treatment was examined.METHODS HCC patient gene expression and clinical data were downloaded from The Cancer Genome Atlas database,and univariate and least absolute shrinkage and selection operator Cox regression models were employed to explore the TRP-related risk spectrum.Based on these analyses,clinically relevant TRP family genes were selected,and the association between the key TRP canonical type 1(TRPC1)gene and HCC patient prognosis was evaluated.RESULTS In total,28 TRP family genes were screened for clinical relevance,with multivariate analyses ultimately revealing three of these genes(TRPC1,TRP cation channel subfamily M member 2,and TRP cation channel subfamily M member 6)to be significantly associated with HCC patient prognosis(P<0.05).These genes were utilized to establish a TRP-related risk model.Patients were separated into low-and high-risk groups based on the expression of these genes,and high-risk patients exhibited a significantly poorer prognosis(P=0.001).Functional analyses highlighted pronounced differences in the immune status of patients in these two groups and associated enriched immune pathways.TRPC1 was identified as a candidate gene in this family worthy of further study,with HCC patients expressing higher TRPC1 levels exhibiting poorer survival outcomes.Consistently,quantitative,immunohistochemistry,and western blot analyses revealed increased TRPC1 expression in HCC.CONCLUSION These three TRP genes help determine HCC patient prognosis,providing insight into tumor immune status and immunological composition.These findings will help design combination therapies including immunotherapeutic and anti-TRP agents. 展开更多
关键词 transient receptor potential family genes Hepatocellular carcinoma transient receptor potential canonical type 1 Novel oncogene
下载PDF
Measuring Ca^(2+) influxes of TRPC1-dependent Ca^(2+) channels in HL-7702 cells with Non-invasive Micro-test Technique 被引量:4
2
作者 Zhen-Ya Zhang Wen-Jun Wang +2 位作者 Li-Jie Pan Yue Xu Zong-Ming Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第33期4150-4155,共6页
AIM: To explore the possibility of using the Noninvasive Micro-test Technique (NMT) to investigate the role of Transient Receptor Potential Canonical 1 (TRPC1) in regulating Ca^2+ influxes in HL-7702 cells, a no... AIM: To explore the possibility of using the Noninvasive Micro-test Technique (NMT) to investigate the role of Transient Receptor Potential Canonical 1 (TRPC1) in regulating Ca^2+ influxes in HL-7702 cells, a normal human liver cell line.METHODS: Net Ca^2+ fluxes were measured with NMT, a technology that can obtain dynamic information of specific/selective ionic/molecular activities on material surfaces, non-invasively. The expression levels of TRPCl were increased by liposomal transfection, whose effectiveness was evaluated by Western-blotting and single cell reverse transcription-polymerase chain reaction.RESULTS: Ca^2+ influxes could be elicited by adding 1 mmol/L CaCl2 to the test solution of HL-7702 cells. They were enhanced by addition of 20 μmol/L noradrenalin and inhibited by 100 μmol/L LaCl3 (a non-selective Ca^2+ channel blocker); 5 μmol/L nifedipine did not induce any change. Overexpression of TRPCl caused increased Ca^2+ influx. Five micromoles per liter nifedipine did not inhibit this elevation, whereas 100 μmol/L LaCI3 did.CONCLUSION: In HL-7702 cells, there is a type of TRPCl-dependent Ca^2+ channel, which could be detected v/a NMT and inhibited by La^3+. 展开更多
关键词 Non-invasive Micro-test Technique Ca^2+ channels transient receptor potential canonical 1 Gene expression HL-7702 cells
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部