Transitional cell carcinoma （TCC） of the ovary is a rare and recently recognized subtype of ovarian epithelial cancer. We presented the first case report from our Institute （Sheri Kashmir Instiute of Medical Scienc...Transitional cell carcinoma （TCC） of the ovary is a rare and recently recognized subtype of ovarian epithelial cancer. We presented the first case report from our Institute （Sheri Kashmir Instiute of Medical Sciences, Srinagar, India）, which was initially rnisdiagnosed as stromal cell carcinoma （granulosa cell tumour）, and on review of histopathology with immunohistochemistry, the diagnosis of TCC of the ovary was established. The aim of this article was to describe the typical case of primary TCC of the ovary and to review the literature for information on TCC management.展开更多
目的研究一种新的凋亡抑制蛋白(Inhibitor of Apoptosis Proten,IAP)livin两种异构体livin-α和livin-βmRNA在膀胱移行细胞癌(Transitional Cell Carcinoma of Bladder,TCC)组织中的表达,及其与TCC的病理分级、临床分期的相关性。方法...目的研究一种新的凋亡抑制蛋白(Inhibitor of Apoptosis Proten,IAP)livin两种异构体livin-α和livin-βmRNA在膀胱移行细胞癌(Transitional Cell Carcinoma of Bladder,TCC)组织中的表达,及其与TCC的病理分级、临床分期的相关性。方法采用逆转录聚合酶链反应(RT-PCR)法检测30例TCC患者的肿瘤组织、3例良性前列腺增生症(Benign Prostatic Hyperplasia,BPH)和2例外伤所致的膀胱破裂患者的膀胱组织中livin-α和livin-βmRNA的表达。结果30例TCC组织中livin mRNA表达阳性率为16.7%,其表达阳性的病例病理分级均为Ⅲ级,肿瘤均侵及深肌层。而3例BPH患者和2例膀胱破裂患者的膀胱组织livin mRNA表达均呈阴性。结论livin可作为TCC的分子标记物,可成为对TCC恶性程度和预后判断的参考指标。展开更多
目的建立快速用于筛查膀胱移行细胞癌(transitional cell carcinoma of bladder,TCC)的尿液细胞角蛋白20(CK20)单克隆抗体胶体金检测试条的方法。方法自行研制CK20单克隆抗体,建立CK20单克隆抗体胶体金试条,对膀胱移行细胞癌、泌尿系统...目的建立快速用于筛查膀胱移行细胞癌(transitional cell carcinoma of bladder,TCC)的尿液细胞角蛋白20(CK20)单克隆抗体胶体金检测试条的方法。方法自行研制CK20单克隆抗体,建立CK20单克隆抗体胶体金试条,对膀胱移行细胞癌、泌尿系统良性疾病和健康人尿液进行检测。结果成功获得一株稳定分泌抗CK20单克隆抗体的杂交瘤细胞,其腹水抗体和纯化的抗体效价均为1∶106,Western-blotting显示得到纯化CK20单克隆抗体。CK20试条检测结果:50例膀胱移行细胞癌患者尿样检出阳性率为78%(39/50),特异性为87%。20例泌尿系统良性疾病患者血尿检测的阳性率是25%(5/20),20例健康人尿液检测均阴性。肿瘤组与对照组敏感性之间差异具有统计学意义(P<0.005)。结论CK20试条法具有特异性好、敏感性高、操作简单、快速和一般实验室可开展的特点,可作为膀胱移行细胞癌快速筛查和疗效观察的一种新方法。展开更多
文摘Transitional cell carcinoma （TCC） of the ovary is a rare and recently recognized subtype of ovarian epithelial cancer. We presented the first case report from our Institute （Sheri Kashmir Instiute of Medical Sciences, Srinagar, India）, which was initially rnisdiagnosed as stromal cell carcinoma （granulosa cell tumour）, and on review of histopathology with immunohistochemistry, the diagnosis of TCC of the ovary was established. The aim of this article was to describe the typical case of primary TCC of the ovary and to review the literature for information on TCC management.
文摘目的建立快速用于筛查膀胱移行细胞癌(transitional cell carcinoma of bladder,TCC)的尿液细胞角蛋白20(CK20)单克隆抗体胶体金检测试条的方法。方法自行研制CK20单克隆抗体,建立CK20单克隆抗体胶体金试条,对膀胱移行细胞癌、泌尿系统良性疾病和健康人尿液进行检测。结果成功获得一株稳定分泌抗CK20单克隆抗体的杂交瘤细胞,其腹水抗体和纯化的抗体效价均为1∶106,Western-blotting显示得到纯化CK20单克隆抗体。CK20试条检测结果:50例膀胱移行细胞癌患者尿样检出阳性率为78%(39/50),特异性为87%。20例泌尿系统良性疾病患者血尿检测的阳性率是25%(5/20),20例健康人尿液检测均阴性。肿瘤组与对照组敏感性之间差异具有统计学意义(P<0.005)。结论CK20试条法具有特异性好、敏感性高、操作简单、快速和一般实验室可开展的特点,可作为膀胱移行细胞癌快速筛查和疗效观察的一种新方法。
