Corneal diseases are a major cause of blindness in the world. Although great progress has been achieved in the treatment of corneal diseases, wound healing after severe corneal damage and immunosuppressive therapy aft...Corneal diseases are a major cause of blindness in the world. Although great progress has been achieved in the treatment of corneal diseases, wound healing after severe corneal damage and immunosuppressive therapy after corneal transplantation remain prob-lematic. Mesenchymal stem cells(MSCs) derived from bone marrow or other adult tissues can differentiate into various types of mesenchymal lineages, such as osteocytes, adipocytes, and chondrocytes, both in vivo and in vitro. These cells can further differentiate into specific cell types under specific conditions. MSCs migrate to injury sites and promote wound healing by secreting anti-inflammatory and growth factors. In ad-dition, MSCs interact with innate and acquired immune cells and modulate the immune response through their powerful paracrine function. Over the last decade, MSCs have drawn considerable attention because of their beneficial properties and promising therapeutic prospective. Furthermore, MSCs have been applied to various studies related to wound healing, autoim-mune diseases, and organ transplantation. This review discusses the potential functions of MSCs in protecting corneal tissue and their possible mechanisms in corneal wound healing and corneal transplantation.展开更多
Major advances are currently being made in regenerative medicine for cornea. Stem cell-based therapies represent a novel strategy that may substitute conventional corneal transplantation, albeit there aremany challeng...Major advances are currently being made in regenerative medicine for cornea. Stem cell-based therapies represent a novel strategy that may substitute conventional corneal transplantation, albeit there aremany challenges ahead given the singularities of each cellular layer of the cornea. This review recapitulates the current data on corneal epithelial stem cells, corneal stromal stem cells and corneal endothelial cell progenitors. Corneal limbal autografts containing epithelial stem cells have been transplanted in humans for more than 20 years with great successful rates, and researchers now focus on ex vivo cultures and other cell lineages to transplant to the ocular surface. A small population of cells in the corneal endothelium was recently reported to have self-renewal capacity, although they do not proliferate in vivo. Two main obstacles have hindered endothelial cell transplantation to date: culture protocols and cell delivery methods to the posterior cornea in vivo. Human corneal stromal stem cells have been identified shortly after the recognition of precursors of endothelial cells. Stromal stem cells may have the potential to provide a direct cell-based therapeutic approach when injected to corneal scars. Furthermore, they exhibit the ability to deposit organized connective tissue in vitro and may be useful in corneal stroma engineering in the future. Recent advances and future perspectives in the field are discussed.展开更多
目的:探讨不同球结膜移植术及术后干预对翼状胬肉患者舒适度及疗效的影响。方法:选取2017年1月—2019年12月样本医院收治的200例(200眼)翼状胬肉患者作为研究对象,按随机数表法分为A、B、C、D 4组,每组50例(50眼)。A、B组采用切除术联...目的:探讨不同球结膜移植术及术后干预对翼状胬肉患者舒适度及疗效的影响。方法:选取2017年1月—2019年12月样本医院收治的200例(200眼)翼状胬肉患者作为研究对象,按随机数表法分为A、B、C、D 4组,每组50例(50眼)。A、B组采用切除术联合自体游离结膜瓣移植术,C、D组采用切除术联合角膜缘干细胞移植,B、D组术后佩戴角膜绷带镜。观察对比4组患者术后眼部不适、视力、角膜上皮愈合时间、治愈率及复发率情况。结果:4组患者术后均出现不同程度的眼部不适,3 d后缓解,2周后基本消失。术后1 d、3 d、7 d B组患者眼部不适较A组轻,D组患者眼部不适较C组轻,而A组与C组、B组与D组之间比较,差异无统计学意义(F=0.083,P>0.05);术后1 d、3 d 4组患者的裸眼视力均有不同程度的下降。术后1 d、3 d B组患者视力较A组高,D组患者视力较C组高,差异有统计学意义(F=3.026、3.088,P<0.05);4组患者术后角膜上皮愈合约需4~6 d,B组角膜上皮愈合时间较A组短,D组较C组短,差异有统计学意义(F=35.800,P<0.05);4组患者术后治愈率和术后翼状胬肉复发情况比较,差异无统计学意义(χ^(2)=0.632、1.031,P>0.05)。结论:切除术联合角膜缘干细胞移植对翼状胬肉患者治疗效果显著,复发率较低。佩戴角膜绷带镜可减轻术后眼部不适,有利于角膜上皮愈合和早期视力的稳定,对翼状胬肉的治疗具有积极意义。展开更多
OBJECTIVE: To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burn...OBJECTIVE: To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burns. METHODS: The proliferation and differentiation of corneal stem cells in vitro had been examined using colony-forming efficiency and immunohistochemistry. The stem cells had been cultured on amniotic membranes and transplanted to the limbal area for treating corneal burns. RESULTS: Corneal stem cells had a high proliferation capacity in primary and first passage, cytokeratin 3 was not expressed in primary culture but partly in first passage. The stem cells could proliferate to form cell layer on an amniotic membrane. When transplanted, stem cells could survive on limbus. After transplantation, ocular inflammation resolved, the cornea re-epithelialized, the stromal opacity reduced, the superficial neovascularity was lessened and the conjunctival fornix re-established. CONCLUSIONS: Ocular surface conditions could be improved by allograft of corneal stem cells cultured on amniotic membranes.展开更多
目的:探究角膜缘干细胞移植术与羊膜移植术联合胬肉切除对翼状胬肉患者角膜上皮修复的影响及其美学效果。方法:选取2019年3月-2021年3月于笔者医院就诊的82例翼状胬肉患者的临床资料,根据手术方式分为羊膜组(n=40)和角膜组(n=42)。统计...目的:探究角膜缘干细胞移植术与羊膜移植术联合胬肉切除对翼状胬肉患者角膜上皮修复的影响及其美学效果。方法:选取2019年3月-2021年3月于笔者医院就诊的82例翼状胬肉患者的临床资料,根据手术方式分为羊膜组(n=40)和角膜组(n=42)。统计两组患者围术期指标,比较两组患者术前及术后3个月眼表泪液指标、视力指标、角膜上皮功能,对比两组患者术后3个月后美学效果,并记录两组患者术后3个月内不良反应发生情况。结果:角膜组患者手术时长长于羊膜组(P<0.05);角膜组患者眼部刺激症状消失时间、角膜上皮修复时间短于羊膜组(P<0.05);术后3个月后,两组患者泪膜破裂时间(Break-up time,BUT)、基础泪液分泌实验(Schirmer I test,SIT)结果高于术前,且角膜组高于羊膜组(P<0.05);术后3个月后,两组患者裸眼视力(Uncorrectedvisualacuity,UCVA)、角膜散光度及角膜上皮荧光素染色评分(Fluorescent,FL)均低于术前,且角膜组低于羊膜组(P<0.05);术后3个月,角膜组美学优良率高于羊膜组(P<0.05);术后3个月内,两组患者不良事件发生率比较差异无统计学意义(P>0.05)。结论:角膜缘干细胞移植术与羊膜移植术在翼状胬肉患者的临床治疗中均可取得较好的治疗及美学效果,且可有效改善患者眼表泪液系统及角膜上皮功能,具有较好的安全性,但角膜缘干细胞移植术对患者各项指标改善效果更佳。展开更多
文摘Corneal diseases are a major cause of blindness in the world. Although great progress has been achieved in the treatment of corneal diseases, wound healing after severe corneal damage and immunosuppressive therapy after corneal transplantation remain prob-lematic. Mesenchymal stem cells(MSCs) derived from bone marrow or other adult tissues can differentiate into various types of mesenchymal lineages, such as osteocytes, adipocytes, and chondrocytes, both in vivo and in vitro. These cells can further differentiate into specific cell types under specific conditions. MSCs migrate to injury sites and promote wound healing by secreting anti-inflammatory and growth factors. In ad-dition, MSCs interact with innate and acquired immune cells and modulate the immune response through their powerful paracrine function. Over the last decade, MSCs have drawn considerable attention because of their beneficial properties and promising therapeutic prospective. Furthermore, MSCs have been applied to various studies related to wound healing, autoim-mune diseases, and organ transplantation. This review discusses the potential functions of MSCs in protecting corneal tissue and their possible mechanisms in corneal wound healing and corneal transplantation.
文摘Major advances are currently being made in regenerative medicine for cornea. Stem cell-based therapies represent a novel strategy that may substitute conventional corneal transplantation, albeit there aremany challenges ahead given the singularities of each cellular layer of the cornea. This review recapitulates the current data on corneal epithelial stem cells, corneal stromal stem cells and corneal endothelial cell progenitors. Corneal limbal autografts containing epithelial stem cells have been transplanted in humans for more than 20 years with great successful rates, and researchers now focus on ex vivo cultures and other cell lineages to transplant to the ocular surface. A small population of cells in the corneal endothelium was recently reported to have self-renewal capacity, although they do not proliferate in vivo. Two main obstacles have hindered endothelial cell transplantation to date: culture protocols and cell delivery methods to the posterior cornea in vivo. Human corneal stromal stem cells have been identified shortly after the recognition of precursors of endothelial cells. Stromal stem cells may have the potential to provide a direct cell-based therapeutic approach when injected to corneal scars. Furthermore, they exhibit the ability to deposit organized connective tissue in vitro and may be useful in corneal stroma engineering in the future. Recent advances and future perspectives in the field are discussed.
文摘目的:探讨不同球结膜移植术及术后干预对翼状胬肉患者舒适度及疗效的影响。方法:选取2017年1月—2019年12月样本医院收治的200例(200眼)翼状胬肉患者作为研究对象,按随机数表法分为A、B、C、D 4组,每组50例(50眼)。A、B组采用切除术联合自体游离结膜瓣移植术,C、D组采用切除术联合角膜缘干细胞移植,B、D组术后佩戴角膜绷带镜。观察对比4组患者术后眼部不适、视力、角膜上皮愈合时间、治愈率及复发率情况。结果:4组患者术后均出现不同程度的眼部不适,3 d后缓解,2周后基本消失。术后1 d、3 d、7 d B组患者眼部不适较A组轻,D组患者眼部不适较C组轻,而A组与C组、B组与D组之间比较,差异无统计学意义(F=0.083,P>0.05);术后1 d、3 d 4组患者的裸眼视力均有不同程度的下降。术后1 d、3 d B组患者视力较A组高,D组患者视力较C组高,差异有统计学意义(F=3.026、3.088,P<0.05);4组患者术后角膜上皮愈合约需4~6 d,B组角膜上皮愈合时间较A组短,D组较C组短,差异有统计学意义(F=35.800,P<0.05);4组患者术后治愈率和术后翼状胬肉复发情况比较,差异无统计学意义(χ^(2)=0.632、1.031,P>0.05)。结论:切除术联合角膜缘干细胞移植对翼状胬肉患者治疗效果显著,复发率较低。佩戴角膜绷带镜可减轻术后眼部不适,有利于角膜上皮愈合和早期视力的稳定,对翼状胬肉的治疗具有积极意义。
文摘OBJECTIVE: To investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burns. METHODS: The proliferation and differentiation of corneal stem cells in vitro had been examined using colony-forming efficiency and immunohistochemistry. The stem cells had been cultured on amniotic membranes and transplanted to the limbal area for treating corneal burns. RESULTS: Corneal stem cells had a high proliferation capacity in primary and first passage, cytokeratin 3 was not expressed in primary culture but partly in first passage. The stem cells could proliferate to form cell layer on an amniotic membrane. When transplanted, stem cells could survive on limbus. After transplantation, ocular inflammation resolved, the cornea re-epithelialized, the stromal opacity reduced, the superficial neovascularity was lessened and the conjunctival fornix re-established. CONCLUSIONS: Ocular surface conditions could be improved by allograft of corneal stem cells cultured on amniotic membranes.
文摘目的:探究角膜缘干细胞移植术与羊膜移植术联合胬肉切除对翼状胬肉患者角膜上皮修复的影响及其美学效果。方法:选取2019年3月-2021年3月于笔者医院就诊的82例翼状胬肉患者的临床资料,根据手术方式分为羊膜组(n=40)和角膜组(n=42)。统计两组患者围术期指标,比较两组患者术前及术后3个月眼表泪液指标、视力指标、角膜上皮功能,对比两组患者术后3个月后美学效果,并记录两组患者术后3个月内不良反应发生情况。结果:角膜组患者手术时长长于羊膜组(P<0.05);角膜组患者眼部刺激症状消失时间、角膜上皮修复时间短于羊膜组(P<0.05);术后3个月后,两组患者泪膜破裂时间(Break-up time,BUT)、基础泪液分泌实验(Schirmer I test,SIT)结果高于术前,且角膜组高于羊膜组(P<0.05);术后3个月后,两组患者裸眼视力(Uncorrectedvisualacuity,UCVA)、角膜散光度及角膜上皮荧光素染色评分(Fluorescent,FL)均低于术前,且角膜组低于羊膜组(P<0.05);术后3个月,角膜组美学优良率高于羊膜组(P<0.05);术后3个月内,两组患者不良事件发生率比较差异无统计学意义(P>0.05)。结论:角膜缘干细胞移植术与羊膜移植术在翼状胬肉患者的临床治疗中均可取得较好的治疗及美学效果,且可有效改善患者眼表泪液系统及角膜上皮功能,具有较好的安全性,但角膜缘干细胞移植术对患者各项指标改善效果更佳。
