Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In ...Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In this study, we evaluated the inhibitory effect of and the mechanism underlying the effect of TJF extract on adipogenesis in 3T3-L1 cells. The effects of TJF extract on cell viability were analyzed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, and the anti-adipogenic effect was measured by oil red O staining. The expression of peroxisomal proliferator activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-α (C/EBP)α, adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), adiponectin, and fatty acid binding protein (FABP)4 involved in adipogenesis was determined by western blot analysis. TJF extract effectively inhibited lipid accumulation and the expression of PPARγ and C/EBPα in 3T3-L1 cells. TJF also increased the phosphorylation of AMPK and ACC, and decreased the expression of adiponectin and FABP4. These results indicate that TJF extract exerts its anti-obesity effect through the downregulation of adipogenic transcription factors and adipogenic marker genes.展开更多
The present study screens activity of different enzymes in Trapa natans under aerobic and anaerobic physiological conditions. Experimental analysis was carried out to assay the activities of ALDH and ADH in the seed e...The present study screens activity of different enzymes in Trapa natans under aerobic and anaerobic physiological conditions. Experimental analysis was carried out to assay the activities of ALDH and ADH in the seed extract of T. natans. These enzymes are associated with the alcohol metabolism of the T. natans. It was observed that a metabolic pathway shifts from aerobic respiration to anaerobic fermentation under anoxic conditions. Physiological stress related response in trapa seeds gives the leads to understand the other model for better fermentable products. During the experiment it was found that there is difference between the concenteration of enzymes in anaerobic and aerobic conditions. Concentration was check with the help of spectrophotometer at 340 nm.展开更多
The low-cost activated carbon was prepared from a renewable aquatic plant residue,Trapa natans husk,and tested for its ability to remove norfloxacin (NOR) from aqueous solutions. Physical and chemical properties of th...The low-cost activated carbon was prepared from a renewable aquatic plant residue,Trapa natans husk,and tested for its ability to remove norfloxacin (NOR) from aqueous solutions. Physical and chemical properties of the Trapa natans husk activated carbon (TAC) were characterized. TAC has a large surface area of 1274 m2/g and mesoporous structure. Carboxylic and hydroxyl groups contributed to the sorption of NOR onto TAC but they were not the most important factors in the sorption process. The rates of adsorption followed the pseudo-second-order kinetics and the overall rate of NOR uptake was controlled by both external mass transfer and intro particle diffusion during the entire adsorption period. The equilibrium data fitted well with the Freundlich and Tempkin models and the sorption was found to be a favorable process. The adsorption of NOR by TAC was strongly dependent on the solution pH. Electrostatic interaction and hydrophobic interaction were proposed to be the principal NOR sorption mechanism.展开更多
文摘Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In this study, we evaluated the inhibitory effect of and the mechanism underlying the effect of TJF extract on adipogenesis in 3T3-L1 cells. The effects of TJF extract on cell viability were analyzed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, and the anti-adipogenic effect was measured by oil red O staining. The expression of peroxisomal proliferator activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-α (C/EBP)α, adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), adiponectin, and fatty acid binding protein (FABP)4 involved in adipogenesis was determined by western blot analysis. TJF extract effectively inhibited lipid accumulation and the expression of PPARγ and C/EBPα in 3T3-L1 cells. TJF also increased the phosphorylation of AMPK and ACC, and decreased the expression of adiponectin and FABP4. These results indicate that TJF extract exerts its anti-obesity effect through the downregulation of adipogenic transcription factors and adipogenic marker genes.
文摘The present study screens activity of different enzymes in Trapa natans under aerobic and anaerobic physiological conditions. Experimental analysis was carried out to assay the activities of ALDH and ADH in the seed extract of T. natans. These enzymes are associated with the alcohol metabolism of the T. natans. It was observed that a metabolic pathway shifts from aerobic respiration to anaerobic fermentation under anoxic conditions. Physiological stress related response in trapa seeds gives the leads to understand the other model for better fermentable products. During the experiment it was found that there is difference between the concenteration of enzymes in anaerobic and aerobic conditions. Concentration was check with the help of spectrophotometer at 340 nm.
基金support from the National Key Technology R&D Program for the Eleventh Five-year Plan (2006BAC10B03)Major National Science and Technology Project (2009ZX07210-009)Graduate Independent Innovation Foundation of Shandong University (2009JQ009)
文摘The low-cost activated carbon was prepared from a renewable aquatic plant residue,Trapa natans husk,and tested for its ability to remove norfloxacin (NOR) from aqueous solutions. Physical and chemical properties of the Trapa natans husk activated carbon (TAC) were characterized. TAC has a large surface area of 1274 m2/g and mesoporous structure. Carboxylic and hydroxyl groups contributed to the sorption of NOR onto TAC but they were not the most important factors in the sorption process. The rates of adsorption followed the pseudo-second-order kinetics and the overall rate of NOR uptake was controlled by both external mass transfer and intro particle diffusion during the entire adsorption period. The equilibrium data fitted well with the Freundlich and Tempkin models and the sorption was found to be a favorable process. The adsorption of NOR by TAC was strongly dependent on the solution pH. Electrostatic interaction and hydrophobic interaction were proposed to be the principal NOR sorption mechanism.
