Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryoprotectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedica...Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryoprotectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedical applications.Trehalose,a nonreducing disaccharide that accumulates in desiccation-or cold-tolerant organisms,has been considered as a biocompatible cryoprotectant.Herein,a naturally occurring membrane-active cationic peptide,melittin,was utilized to facilitate membraneimpermeable trehalose entry into cells for effective cell cryopreservation.Poly(ethylene glycol)and poly(vinyl pyrrolidone)were applied as macromolecular protectants to improve the stabilization of cell membranes.Upon the optimal protocol,the postthaw recovery of human red blood cells in freezing bags at a hematocrit of~50%could achieve 82.9%with favorable cell morphologies and physiological functions.Furthermore,the cryosurvival of L929 fibroblasts reached 84.3%compared to the conventional method using 10%(v/v)DMSO.In short,this work by using trehalose and melittin provides a biocompatible solvent-free approach for long-term cryostorage of cells.展开更多
The synthesized C-phycocyanins （C-PCs） doped silica biomaterials were characterized by the SEM and BET surface area analysis measurement. The morphology of C-PCs doped silica biomaterials indicates that the surface ...The synthesized C-phycocyanins （C-PCs） doped silica biomaterials were characterized by the SEM and BET surface area analysis measurement. The morphology of C-PCs doped silica biomaterials indicates that the surface of the silica cluster is formed by a great number of silica particles with an average size of between 30 and 40 nm. Silica itself is a porous structure with the average pore diameter of 2.95 nm. Pores with their diameter less than 5 nm account for 84.07%. In addition, the C-PCs can be utilized as a fluorescent protein probe to monitor influence of the protein encapsulation and to study matrix and protein interaction and stability of protein in silica matrix. Application of protein encapsulation silica materials requires biomolecules to keep bioactivity and stability on potentially unfavorable industrial conditions. The C-PCs in solution or in silicate matrix irradiated by ultraviolet ray can result in photobleaching, whereas the protein in the silica is less affected. The measured photodamage rate constant of C-PCs in buffer solution is 25 times faster than that of C-PCs in silica matrix. However, the lifetime of C-PCs in silica matrix or phosphate buffer is unaffected. These studies suggest that entrapment of C-PCs into silica matrixes not only can maintain their biological activity but also noticeably improve their photostability.展开更多
文摘Cryopreservation plays an essential role in biobanking and cell therapy,but the physiological toxicity of traditional cryoprotectants such as glycerol and dimethyl sulfoxide(DMSO)has raised safety issues for biomedical applications.Trehalose,a nonreducing disaccharide that accumulates in desiccation-or cold-tolerant organisms,has been considered as a biocompatible cryoprotectant.Herein,a naturally occurring membrane-active cationic peptide,melittin,was utilized to facilitate membraneimpermeable trehalose entry into cells for effective cell cryopreservation.Poly(ethylene glycol)and poly(vinyl pyrrolidone)were applied as macromolecular protectants to improve the stabilization of cell membranes.Upon the optimal protocol,the postthaw recovery of human red blood cells in freezing bags at a hematocrit of~50%could achieve 82.9%with favorable cell morphologies and physiological functions.Furthermore,the cryosurvival of L929 fibroblasts reached 84.3%compared to the conventional method using 10%(v/v)DMSO.In short,this work by using trehalose and melittin provides a biocompatible solvent-free approach for long-term cryostorage of cells.
基金Funded by the National Natural Science Foundation of China (No. 20873005)Beijing Natural Science Fundation (No.2083028)+1 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry422 Funds from University of Science and Technology Beijing
文摘The synthesized C-phycocyanins （C-PCs） doped silica biomaterials were characterized by the SEM and BET surface area analysis measurement. The morphology of C-PCs doped silica biomaterials indicates that the surface of the silica cluster is formed by a great number of silica particles with an average size of between 30 and 40 nm. Silica itself is a porous structure with the average pore diameter of 2.95 nm. Pores with their diameter less than 5 nm account for 84.07%. In addition, the C-PCs can be utilized as a fluorescent protein probe to monitor influence of the protein encapsulation and to study matrix and protein interaction and stability of protein in silica matrix. Application of protein encapsulation silica materials requires biomolecules to keep bioactivity and stability on potentially unfavorable industrial conditions. The C-PCs in solution or in silicate matrix irradiated by ultraviolet ray can result in photobleaching, whereas the protein in the silica is less affected. The measured photodamage rate constant of C-PCs in buffer solution is 25 times faster than that of C-PCs in silica matrix. However, the lifetime of C-PCs in silica matrix or phosphate buffer is unaffected. These studies suggest that entrapment of C-PCs into silica matrixes not only can maintain their biological activity but also noticeably improve their photostability.
