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Derivative Spectroscopy and its Application at Detecting the Weak Emission/Absorption Lines
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作者 Lihuan Yu Jiangdan Li +4 位作者 Jinliang Wang Jiajia Li Jiao Li Qiang Xi Zhanwen Han 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第3期205-223,共19页
The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened ... The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line. 展开更多
关键词 line identification-line profiles-methods data analysis-techniques spectroscopic-techniques radial velocities
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Experimental,Numerical,and Analytical Studies on the Bending of Mechanically Lined Pipe
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作者 WEI Wen-bin YUAN Lin +1 位作者 ZHOU Jia-sheng LIU Zheng 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期221-232,共12页
Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which cau... Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which causes the liner to separate from the outer pipe and buckles,affecting the stability of the whole line.In this paper,the buckling response of MLP subjected to bending is investigated to clarify its bending characteristics by employing both experiments,numerical simulation,as theoretical methods.Two types of MLPs were manufactured with GB 45 carbon steel(SLP)and Al 6061(ALP)used as the outer pipe material,respectively.The hydraulic expansion and bending experiments of small-scale MLPs are conducted.In addition to the ovalized shape of the cross-section for the SLP specimens,the copper liner was found to wrinkle on the compressive side.In contrast,the liner of ALP remains intact without developing any wrinkling and collapse mode.In addition,a dedicated numerical framework and theoretical models were also established.It was found both the manufacturing and bending responses of the MLP can be well reproduced,and the predicted maximum moment and critical curvatures are in good agreement with the experimental results. 展开更多
关键词 lined pipe BENDING nonlinear ring theory BUCKLING PLASTICITY
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Petrology of Spinel-Lherzolite Xenoliths from Mazéléand Others Northen Xenoliths Localities of Cameroon Volcanic Line: Exchange Reactions and Equilibrium State
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作者 Nguihdama Dagwai Kamgang Pierre +2 位作者 Mbowou Gbambié Isaac Bertrand Chazot Gilles Ngounouno Ismaïla 《Open Journal of Geology》 CAS 2024年第5期629-653,共25页
The alkaline volcanism of the Cameroon Volcanic Line in its northern domain has raised many fresh enclaves of peridotites. The samples selected come from five (05) different localities (Liri, in the plateau of Kapsiki... The alkaline volcanism of the Cameroon Volcanic Line in its northern domain has raised many fresh enclaves of peridotites. The samples selected come from five (05) different localities (Liri, in the plateau of Kapsiki, Mazélé in the NE of Ngaoundéré, Tello and Ganguiré in the SE of Ngaoundéré and Likok, locality located in the west of Ngaoundé). The peridotite enclaves of the above localities show restricted mineralogical variation. Most are four-phase spinel-lherzolites, indicating that this is the main lithology that forms the lithospheric mantle below the shallow zone. No traces of garnet or primary plagioclase were detected, which strongly limits the depth range from which the rock fragments were sampled. The textures and the wide equilibrium temperatures (884˚C - 1115˚C) indicate also entrainment of lherzolite xenoliths from shallow depths within the lithosphere and the presence of mantle diapirism. The exchange reactions and equilibrium state established in this work make it possible to characterize the chemical composition of the upper mantle of each region and test the equilibrium state of the phases between them. Variations of major oxides and incompatible elemental concentrations in clinopyroxene indicate a primary control by partial melting. The absence of typical “metasomatic” minerals, low equilibration temperatures and enriched LREE patterns indicate that the upper mantle below septentrional crust of Cameroun underwent an event of cryptic metasomatic enrichment prior to partial melting. The distinctive chemical features, LREE enrichment, strong U, Ce and Pr, depletion relative to Ba, Nb, La, Pb, and T, fractionation of Zr and Hf and therefore ligh high Zr/Hf ratio, low La/Yb, Nb/La and Ti/Eu are all results of interaction of refractory peridotite residues with carbonatite melts. 展开更多
关键词 XENOLITHS Upper Mantle Northern Region Adamawa Cameroon Volcanic line Exchange Reaction
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Evaluation of red soil-bentonite mixtures for compacted clay liners
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作者 A.S.Devapriya T.Thyagaraj 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期697-710,共14页
Compacted clay liners are an integral part of the waste landfills,which are provided to contain the leachate within the landfills and protect the surrounding environment.Generally,locally available natural soils are u... Compacted clay liners are an integral part of the waste landfills,which are provided to contain the leachate within the landfills and protect the surrounding environment.Generally,locally available natural soils are used for the construction of compacted clay liners if they satisfy the design criteria.However,not all soils in their natural state satisfy all the design criteria for the liner materials.Thus,there is a definite need to modify the locally available natural soils by blending with bentonite to meet the required design criteria for the liners.In view of this,the present study evaluates the suitability of an Indian red soil enhanced with bentonite as a liner material.To achieve this,a series of experiments were carried out using locally available red soil and bentonite.First,the suitability of the red soil was evaluated as a liner material.The experimental results showed that the red soil met all the selection criteria stipulated by the Environmental Protection Agencies(EPAs)for the liners except the hydraulic conductivity criterion.Therefore,the red soil was mixed with bentonite contents of 10%,20%and 30%,and the red soil-bentonite mixtures were evaluated for their suitability for liners in their compacted state.Further,as the liners in the arid and semi-arid regions are subjected to moisture variations due to seasonal moisture fluctuations and other factors,the red soil-bentonite mixtures were subjected to wetdry cycles,and their suitability was evaluated after wet-dry cycles.The experimental results revealed that all the red soil-bentonite mixtures met the stipulated EPA criteria for the liners in the as-compacted state.However,the red soil-bentonite mixtures with 20%and 30%bentonite contents only satisfied the hydraulic conductivity requirement even after wet-dry cycles.The experimental findings were supplemented with the microstructural insights captured through digital camera images,scanning electron microscopy(SEM),and mercury intrusion porosimetry(MIP)studies. 展开更多
关键词 Compacted clay lines Hydraulic conductivity Wet-dry cycles Microstructure
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ST-LaneNet: Lane Line Detection Method Based on Swin Transformer and LaneNet
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作者 Yufeng Du Rongyun Zhang +3 位作者 Peicheng Shi Linfeng Zhao Bin Zhang Yaming Liu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第1期130-145,共16页
The advancement of autonomous driving heavily relies on the ability to accurate lane lines detection.As deep learning and computer vision technologies evolve,a variety of deep learning-based methods for lane line dete... The advancement of autonomous driving heavily relies on the ability to accurate lane lines detection.As deep learning and computer vision technologies evolve,a variety of deep learning-based methods for lane line detection have been proposed by researchers in the field.However,owing to the simple appearance of lane lines and the lack of distinctive features,it is easy for other objects with similar local appearances to interfere with the process of detecting lane lines.The precision of lane line detection is limited by the unpredictable quantity and diversity of lane lines.To address the aforementioned challenges,we propose a novel deep learning approach for lane line detection.This method leverages the Swin Transformer in conjunction with LaneNet(called ST-LaneNet).The experience results showed that the true positive detection rate can reach 97.53%for easy lanes and 96.83%for difficult lanes(such as scenes with severe occlusion and extreme lighting conditions),which can better accomplish the objective of detecting lane lines.In 1000 detection samples,the average detection accuracy can reach 97.83%,the average inference time per image can reach 17.8 ms,and the average number of frames per second can reach 64.8 Hz.The programming scripts and associated models for this project can be accessed openly at the following GitHub repository:https://github.com/Duane 711/Lane-line-detec tion-ST-LaneNet. 展开更多
关键词 Autonomous driving Lane line detection Deep learning Swin transformer
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An Implementation of Multiscale Line Detection and Mathematical Morphology for Efficient and Precise Blood Vessel Segmentation in Fundus Images
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作者 Syed Ayaz Ali Shah Aamir Shahzad +4 位作者 Musaed Alhussein Chuan Meng Goh Khursheed Aurangzeb Tong Boon Tang Muhammad Awais 《Computers, Materials & Continua》 SCIE EI 2024年第5期2565-2583,共19页
Diagnosing various diseases such as glaucoma,age-related macular degeneration,cardiovascular conditions,and diabetic retinopathy involves segmenting retinal blood vessels.The task is particularly challenging when deal... Diagnosing various diseases such as glaucoma,age-related macular degeneration,cardiovascular conditions,and diabetic retinopathy involves segmenting retinal blood vessels.The task is particularly challenging when dealing with color fundus images due to issues like non-uniformillumination,low contrast,and variations in vessel appearance,especially in the presence of different pathologies.Furthermore,the speed of the retinal vessel segmentation system is of utmost importance.With the surge of now available big data,the speed of the algorithm becomes increasingly important,carrying almost equivalent weightage to the accuracy of the algorithm.To address these challenges,we present a novel approach for retinal vessel segmentation,leveraging efficient and robust techniques based on multiscale line detection and mathematical morphology.Our algorithm’s performance is evaluated on two publicly available datasets,namely the Digital Retinal Images for Vessel Extraction dataset(DRIVE)and the Structure Analysis of Retina(STARE)dataset.The experimental results demonstrate the effectiveness of our method,withmean accuracy values of 0.9467 forDRIVE and 0.9535 for STARE datasets,aswell as sensitivity values of 0.6952 forDRIVE and 0.6809 for STARE datasets.Notably,our algorithmexhibits competitive performance with state-of-the-art methods.Importantly,it operates at an average speed of 3.73 s per image for DRIVE and 3.75 s for STARE datasets.It is worth noting that these results were achieved using Matlab scripts containing multiple loops.This suggests that the processing time can be further reduced by replacing loops with vectorization.Thus the proposed algorithm can be deployed in real time applications.In summary,our proposed system strikes a fine balance between swift computation and accuracy that is on par with the best available methods in the field. 展开更多
关键词 line detector vessel detection LOCALIZATION mathematical morphology image processing
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On traceable iterated line graph and hamiltonian path index
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作者 NIU Zhao-hong XIONG Li-ming YANG Wei-hua 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2024年第2期239-252,共14页
Xiong and Liu[21]gave a characterization of the graphs G for which the n-iterated line graph L^(n)(G)is hamiltonian,for n≥2.In this paper,we study the existence of a hamiltonian path in L^(n)(G),and give a characteri... Xiong and Liu[21]gave a characterization of the graphs G for which the n-iterated line graph L^(n)(G)is hamiltonian,for n≥2.In this paper,we study the existence of a hamiltonian path in L^(n)(G),and give a characterization of G for which L^(n)(G)has a hamiltonian path.As applications,we use this characterization to give several upper bounds on the hamiltonian path index of a graph. 展开更多
关键词 iterated line graph TRACEABLE hamiltonian index hamiltonian path index
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Learning Epipolar Line Window Attention for Stereo Image Super-Resolution Reconstruction
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作者 Xue Li Hongying Zhang +1 位作者 Zixun Ye Xiaoru 《Computers, Materials & Continua》 SCIE EI 2024年第2期2847-2864,共18页
Transformer-based stereo image super-resolution reconstruction(Stereo SR)methods have significantly improved image quality.However,existing methods have deficiencies in paying attention to detailed features and do not... Transformer-based stereo image super-resolution reconstruction(Stereo SR)methods have significantly improved image quality.However,existing methods have deficiencies in paying attention to detailed features and do not consider the offset of pixels along the epipolar lines in complementary views when integrating stereo information.To address these challenges,this paper introduces a novel epipolar line window attention stereo image super-resolution network(EWASSR).For detail feature restoration,we design a feature extractor based on Transformer and convolutional neural network(CNN),which consists of(shifted)window-based self-attention((S)W-MSA)and feature distillation and enhancement blocks(FDEB).This combination effectively solves the problem of global image perception and local feature attention and captures more discriminative high-frequency features of the image.Furthermore,to address the problem of offset of complementary pixels in stereo images,we propose an epipolar line window attention(EWA)mechanism,which divides windows along the epipolar direction to promote efficient matching of shifted pixels,even in pixel smooth areas.More accurate pixel matching can be achieved using adjacent pixels in the window as a reference.Extensive experiments demonstrate that our EWASSR can reconstruct more realistic detailed features.Comparative quantitative results show that in the experimental results of our EWASSR on the Middlebury and Flickr1024 data sets for 2×SR,compared with the recent network,the Peak signal-to-noise ratio(PSNR)increased by 0.37 dB and 0.34 dB,respectively. 展开更多
关键词 Stereo SR epipolar line window attention feature distillation
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A Simple and Effective Surface Defect Detection Method of Power Line Insulators for Difficult Small Objects
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作者 Xiao Lu Chengling Jiang +2 位作者 Zhoujun Ma Haitao Li Yuexin Liu 《Computers, Materials & Continua》 SCIE EI 2024年第4期373-390,共18页
Insulator defect detection plays a vital role in maintaining the secure operation of power systems.To address the issues of the difficulty of detecting small objects and missing objects due to the small scale,variable... Insulator defect detection plays a vital role in maintaining the secure operation of power systems.To address the issues of the difficulty of detecting small objects and missing objects due to the small scale,variable scale,and fuzzy edge morphology of insulator defects,we construct an insulator dataset with 1600 samples containing flashovers and breakages.Then a simple and effective surface defect detection method of power line insulators for difficult small objects is proposed.Firstly,a high-resolution featuremap is introduced and a small object prediction layer is added so that the model can detect tiny objects.Secondly,a simplified adaptive spatial feature fusion(SASFF)module is introduced to perform cross-scale spatial fusion to improve adaptability to variable multi-scale features.Finally,we propose an enhanced deformable attention mechanism(EDAM)module.By integrating a gating activation function,the model is further inspired to learn a small number of critical sampling points near reference points.And the module can improve the perception of object morphology.The experimental results indicate that concerning the dataset of flashover and breakage defects,this method improves the performance of YOLOv5,YOLOv7,and YOLOv8.In practical application,it can simply and effectively improve the precision of power line insulator defect detection and reduce missing detection for difficult small objects. 展开更多
关键词 Insulator defect detection small object power line deformable attention mechanism
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Rigidity Symmetry Line for Thermodynamic Fluid Equations-of-State
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作者 Leslie V. Woodcock 《Journal of Modern Physics》 2024年第5期613-633,共21页
We report progress towards a modern scientific description of thermodynamic properties of fluids following the discovery (in 2012) of a coexisting critical density hiatus and a supercritical mesophase defined by perco... We report progress towards a modern scientific description of thermodynamic properties of fluids following the discovery (in 2012) of a coexisting critical density hiatus and a supercritical mesophase defined by percolation transitions. The state functions density ρ(p,T), and Gibbs energy G(p,T), of fluids, e.g. CO<sub>2</sub>, H<sub>2</sub>O and argon exhibit a symmetry characterised by the rigidity, ω = (dp/dρ)<sub>T</sub>, between gaseous and liquid states along any isotherm from critical (T<sub>c</sub>) to Boyle (T<sub>B</sub>) temperatures, on either side of the supercritical mesophase. Here, using experimental data for fluid argon, we investigate the low-density cluster physics description of an ideal dilute gas that obeys Dalton’s partial pressure law. Cluster expansions in powers of density relate to a supercritical liquid-phase rigidity symmetry (RS) line (ω = ρ<sub>rs</sub>(T) = RT) to gas phase virial coefficients. We show that it is continuous in all derivatives, linear within stable fluid phase, and relates analytically to the Boyle-work line (BW) (w = (p/ρ)<sub>T</sub> = RT), and to percolation lines of gas (PB) and liquid (PA) phases by: ρ<sub>BW</sub>(T) = 2ρ<sub>PA</sub>(T) = 3ρ<sub>PB</sub>(T) = 3ρ<sub>RS</sub>(T)/2 for T T<sub>B</sub>. These simple relationships arise, because the higher virial coefficients (b<sub>n</sub>, n ≥ 4) cancel due to clustering equilibria, or become negligible at all temperatures (0 T T<sub>B</sub>)<sub> </sub>within the gas phase. The Boyle-work line (p/ρ<sub>BW</sub>)<sub>T</sub> is related exactly at lower densities as T → T<sub>B</sub>, and accurately for liquid densities, by ρ<sub>BW</sub>(T) = −(b<sub>2</sub>/b<sub>3</sub>)<sub>T</sub>. The RS line, ω(T) = RT, defines a new liquid-density ground-state physical constant (ρ<sub>RS</sub>(0) = (2/3)ρ<sub>BW</sub>(0) for argon). Given the gas-liquid rigidity symmetry, the entire thermodynamic state functions below T<sub>B</sub> are obtainable from b<sub>2</sub>(T). A BW-line ground-state crystal density ρ<sub>BW</sub>(0) can be defined by the pair potential minimum. The Ar<sub>2</sub> pair potential, ∅ij</sub>(r<sub>ij</sub>) determines b<sub>2</sub>(T) analytically for all T. This report, therefore, advances the salient objective of liquid-state theory: an argon p(ρ,T) Equation-of-state is obtained from ∅<sub>ij</sub>(r<sub>ij</sub>) for all fluid states, without any adjustable parameters. 展开更多
关键词 Liquid-State Theory Cluster Physics Percolation lines Fluid Thermodynamics Boyle line Rigidity-Symmetry line
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Digital Twin Modeling and Simulation Optimization of Transmission Front and Middle Case Assembly Line
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作者 Xianfeng Cao Meihua Yao +2 位作者 Yahui Zhang Xiaofeng Hu Chuanxun Wu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第6期3233-3253,共21页
As the take-off of China’s macro economy,as well as the rapid development of infrastructure construction,real estate industry,and highway logistics transportation industry,the demand for heavy vehicles is increasing ... As the take-off of China’s macro economy,as well as the rapid development of infrastructure construction,real estate industry,and highway logistics transportation industry,the demand for heavy vehicles is increasing rapidly,the competition is becoming increasingly fierce,and the digital transformation of the production line is imminent.As one of themost important components of heavy vehicles,the transmission front andmiddle case assembly lines have a high degree of automation,which can be used as a pilot for the digital transformation of production.To ensure the visualization of digital twins(DT),consistent control logic,and real-time data interaction,this paper proposes an experimental digital twin modeling method for the transmission front and middle case assembly line.Firstly,theDT-based systemarchitecture is designed,and theDT model is created by constructing the visualization model,logic model,and data model of the assembly line.Then,a simulation experiment is carried out in a virtual space to analyze the existing problems in the current assembly line.Eventually,some improvement strategies are proposed and the effectiveness is verified by a new simulation experiment. 展开更多
关键词 Transmission front and middle case assembly line digital twin(DT) simulating optimization intelligent manufacturing
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Simulation and Experimental Study of Staple Line Reinforcement Surgery
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作者 Chengjie Hu Jian Zhang 《Journal of Biomedical Science and Engineering》 2024年第4期83-95,共13页
The aim of this study was to evaluate the effectiveness of BM (basement membrane) and SIS (small intestine submucosa) composite extracellular matrix staple line reinforcement in surgical procedures through finite elem... The aim of this study was to evaluate the effectiveness of BM (basement membrane) and SIS (small intestine submucosa) composite extracellular matrix staple line reinforcement in surgical procedures through finite element modelling simulations and leak-proof performance experiments. The mechanical analyses of soft tissues with and without staple line reinforcement were performed by establishing finite element models of three tissues, namely, stomach, intestine and lungs, under the use scenarios of different anastomosis staple models;and the leak-proof performance of the staple line reinforcement was evaluated by simulating leak-proof experiments of gastric incision margins, intestinal sections, and lung incision margins in vitro. The results showed that the equivalent average stresses of the staple line reinforcement were increased by 20 kPa-68 kPa in gastric and intestinal tissues, and 8 kPa-22 kPa in lung tissues. and that the BM and SIS composite extracellular matrix staple line reinforcement could strengthen the anastomotic structure, and at the same time disperse the high stresses of the anastomosed tissues, which could effectively reduce the postoperative complications such as anastomotic bleeding and anastomotic leakage, and provide a safer and more effective optimized design for surgical mechanical anastomosis. It can effectively reduce postoperative complications such as anastomotic bleeding and anastomotic leakage, and provide a safer and more effective optimized design for surgical mechanical anastomosis. 展开更多
关键词 Staple line Reinforcement Surgical Anastomosis Soft Tissue Damage Finite Element Analysis Leak-Proof Performance ExperimentsStaple cartridge
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Research on Transmission Line Tower Tilting and Foundation State Monitoring Technology Based onMulti-Sensor Cooperative Detection and Correction
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作者 Guangxin Zhang Minghui Liu +4 位作者 Shichao Cheng Minzhen Wang Changshun Zhao Hongdan Zhao Gaiming Zhong 《Energy Engineering》 EI 2024年第1期169-185,共17页
The transmission line tower will be affected by bad weather and artificial subsidence caused by the foundation and other factors in the power transmission.The tower’s tilt and severe deformation will cause the buildi... The transmission line tower will be affected by bad weather and artificial subsidence caused by the foundation and other factors in the power transmission.The tower’s tilt and severe deformation will cause the building to collapse.Many small changes caused the tower’s collapse,but the early staff often could not intuitively notice the changes in the tower’s state.In the current tower online monitoring system,terminal equipment often needs to replace batteries frequently due to premature exhaustion of power.According to the need for real-time measurement of power line tower,this research designed a real-time monitoring device monitoring the transmission tower attitude tilting and foundation state based on the inertial sensor,the acceleration of 3 axis inertial sensor and angular velocity raw data to pole average filtering pre-processing,and then through the complementary filtering algorithm for comprehensive calculation of tilt angle,the system meets the demand for inclined online monitoring of power line poles and towers regarding measurement accuracy,with low cost and power consumption.The optimization multi-sensor cooperative detection and correction measured tilt angle result relative accuracy can reach 1.03%,which has specific promotion and application value since the system has the advantages of unattended and efficient calculation. 展开更多
关键词 Transmission line tower tilting MULTI-SENSOR foundation state monitoring collaborative detection
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N^(6)-腺苷甲基化修饰及其对LINE-1的调控机制
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作者 张傲 岑山 李晓宇 《遗传》 CAS CSCD 北大核心 2024年第3期209-218,共10页
长散布元件-1(long interspersed elements-1,LINE-1)是现今在人类基因组中唯一具有自主转座能力的转座子,其转座会引起细胞基因组结构和功能的改变,是导致多种严重疾病的重要因素。在转座过程中,LINE-1 mRNA是转座中间体的核心,宿主细... 长散布元件-1(long interspersed elements-1,LINE-1)是现今在人类基因组中唯一具有自主转座能力的转座子,其转座会引起细胞基因组结构和功能的改变,是导致多种严重疾病的重要因素。在转座过程中,LINE-1 mRNA是转座中间体的核心,宿主细胞对其进行相关修饰直接影响转座。N^(6)-腺苷甲基化修饰(m^(6)A)是真核细胞RNA上最丰富且动态可逆的表观遗传修饰。目前发现m^(6)A修饰也存在于LINE-1 mRNA上,参与LINE-1整个生命周期的调控,影响其转座和基因组中LINE-1相邻基因的表达,进而影响基因组稳定性、细胞自我更新与分化潜能,在人类发育和疾病中具有重要作用。本文介绍了LINE-1 m^(6)A修饰的位置、功能以及相关机制,并总结了LINE-1的m^(6)A修饰对其转座调控的研究进展,以期为相关疾病发生发展的机制研究和治疗提供新的思路。 展开更多
关键词 m^(6)A修饰 逆转录转座子 line-1 基因组 基因组稳定性
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Multi-Branch Fault Line Location Method Based on Time Difference Matrix Fitting
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作者 Hua Leng Silin He +3 位作者 Jian Qiu Feng Liu Xinfei Huang Jiran Zhu 《Energy Engineering》 EI 2024年第1期77-94,共18页
The distribution network exhibits complex structural characteristics,which makes fault localization a challenging task.Especially when a branch of the multi-branch distribution network fails,the traditional multi-bran... The distribution network exhibits complex structural characteristics,which makes fault localization a challenging task.Especially when a branch of the multi-branch distribution network fails,the traditional multi-branch fault location algorithm makes it difficult to meet the demands of high-precision fault localization in the multi-branch distribution network system.In this paper,the multi-branch mainline is decomposed into single branch lines,transforming the complex multi-branch fault location problem into a double-ended fault location problem.Based on the different transmission characteristics of the fault-traveling wave in fault lines and non-fault lines,the endpoint reference time difference matrix S and the fault time difference matrix G were established.The time variation rule of the fault-traveling wave arriving at each endpoint before and after a fault was comprehensively utilized.To realize the fault segment location,the least square method was introduced.It was used to find the first-order fitting relation that satisfies the matching relationship between the corresponding row vector and the first-order function in the two matrices,to realize the fault segment location.Then,the time difference matrix is used to determine the traveling wave velocity,which,combined with the double-ended traveling wave location,enables accurate fault location. 展开更多
关键词 Multi-branch lines distribution network fault location double-ended traveling wave positioning least square method
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GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines
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作者 Yang-Zhou Jiang Lan-Yue Hu +11 位作者 Mao-Shan Chen Xiao-Jie Wang Cheng-Ning Tan Pei-Pei Xue Teng Yu Xiao-Yan He Li-Xin Xiang Yan-Ni Xiao Xiao-Liang Li Qian Ran Zhong-Jun Li Li Chen 《World Journal of Stem Cells》 SCIE 2024年第5期538-550,共13页
BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untran... BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown. 展开更多
关键词 Ankyrin repeat domain containing 26 GATA binding protein 2 Thrombocytopenia 2 Transcriptional regulation Myeloid-derived cell lines
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低压低氧对肠黏膜屏障及LINE-1核酸内切酶变异体GCRG213表达的影响
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作者 张王静怡 张少杰 +4 位作者 陶虹锦 孟繁森 刘静 朱玲玲 王刚石 《解放军医学院学报》 CAS 2024年第3期296-301,F0003,共7页
背景长散在核元件-1(long interspersed nuclear element-1,LINE-1或L1)是目前基因组中唯一活跃的自主反转录转座元件,低压低氧环境可以改变L1的甲基化程度。目的探究低压低氧环境对肠黏膜中L1核酸内切酶(L1 endonuclease,L1-EN)变异体G... 背景长散在核元件-1(long interspersed nuclear element-1,LINE-1或L1)是目前基因组中唯一活跃的自主反转录转座元件,低压低氧环境可以改变L1的甲基化程度。目的探究低压低氧环境对肠黏膜中L1核酸内切酶(L1 endonuclease,L1-EN)变异体GCRG213表达水平及以闭合蛋白(Occludin)和紧密连接蛋白-1(Claudin-1)表达水平为指标的肠黏膜机械屏障功能的影响。方法利用免疫组织化学染色(immunohistochemical staining,IHC)检测人正常小肠、结肠组织及小鼠正常结肠组织的GCRG213表达,观察GCRG213蛋白在正常肠道中的分布规律。将雄性C57BL/6小鼠随机分为实验组和对照组。实验组模拟海拔6000 m环境,饲养7 d构建低压低氧小鼠模型,对照组常压常氧饲养。将人正常结肠上皮细胞NCM-460随机分为常氧组以及低氧24 h、48 h组,常氧组于常氧环境正常培养,低氧组分别于0.3%O2浓度下培养24 h、48 h构建低氧细胞模型。使用qPCR、Western blot技术检测GCRG213、Occludin和Claudin-1在小鼠结肠组织及人NCM-460细胞中表达水平的变化。结果人正常小肠、结肠及小鼠结肠组织IHC结果一致显示,GCRG213表达于肠黏膜柱状上皮细胞胞质中,而在杯状细胞中未见表达。在动物实验中,与常氧组相比,低压低氧组小鼠结肠组织GCRG213在mRNA水平(P<0.05)和蛋白水平(P<0.01)均表达上调;同时,Occludin在蛋白水平表达下调(P<0.05)。在细胞实验中,低氧暴露后NCM-460细胞中GCRG213在mRNA(P<0.001)和蛋白水平(P<0.01)均表达上调,其中低氧24 h组GCRG213表达水平低于低氧48 h组,Occludin、Claudin-1在mRNA水平(P<0.01;P<0.001)表达下调,其中低氧24 h组表达水平均低于低氧48 h组,Occludin蛋白水平表达在低氧48 h组出现下调(P<0.05)。结论L1-EN变异体GCRG213在人和小鼠正常结肠组织黏膜柱状上皮细胞胞质内存在表达;在体内外模型中,低压低氧暴露下结肠黏膜上皮细胞GCRG213表达升高,同时观察到低压低氧对结肠黏膜紧密连接完整性产生影响,表现为Occludin、Claudin-1蛋白表达水平的降低。 展开更多
关键词 低压低氧 肠黏膜 紧密连接蛋白 GCRG213 line-1
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Pumpingline管道预制与安装研究——以2023 SH 二次配Pumping-line工程项目为例
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作者 张俊刚 《中国科技期刊数据库 工业A》 2024年第3期0195-0198,共4页
本文以中芯南方集成电路制造有限公司负责的2023?SH?二次配Pumping-line工程项目为例,首先对Pumping line管道预制与安装流程进行了简单介绍。在此基础上,本文围绕机台Pumping line会勘、Pumping line管道测量绘图作业、Pumping line管... 本文以中芯南方集成电路制造有限公司负责的2023?SH?二次配Pumping-line工程项目为例,首先对Pumping line管道预制与安装流程进行了简单介绍。在此基础上,本文围绕机台Pumping line会勘、Pumping line管道测量绘图作业、Pumping line管道的预制与现场安装作业要素、机台Monve-in定位后Hook up相关作业要素等展开分析,希望为相关从业人员提供一定的参考。 展开更多
关键词 PUMPING line管道 会勘 预制 现场安装
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Analysis of the Design and Application of a Novel CT Secondary Cable Line Calibrator
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作者 Jiaxing Ren 《Journal of Electronic Research and Application》 2024年第2期7-11,共5页
With the rapid development of electrical power systems,ensuring the accuracy and reliability of power transmis-sion has become particularly crucial.The secondary cable line calibrator for current transformers(CT)plays... With the rapid development of electrical power systems,ensuring the accuracy and reliability of power transmis-sion has become particularly crucial.The secondary cable line calibrator for current transformers(CT)plays an essential role in calibrating electrical power systems.It is not only related to the safe operation of the system but also directly im-pacts the accuracy of energy metering.This study aims to design and analyze an efficient CT secondary cable line calibra-tor to explore its application effects in the power system.By thoroughly analyzing the characteristics of CT secondary ca-ble lines and the design requirements of the calibrator,this paper proposes an innovative design scheme for the calibrator.This device demonstrates significant effects in enhancing the accuracy and stability of power system calibration,providing robust technical support for the optimization and upgrade of the power system.This research not only offers a theoretical basis and practical guidance for the design and application of CT secondary cable line calibrators but also contributes new ideas and methods for the precise calibration and efficient management of the power system. 展开更多
关键词 CT secondary cable line CALIBRATOR Power system DESIGN Application
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Online-SPE-UPLC-MS/MS检测生活污水中20种毒品
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作者 裴海燕 鄢茜 +5 位作者 周衍霄 张宏建 朱思琪 杨继伟 俞晓峰 赵鹏 《化学研究与应用》 CAS 北大核心 2024年第2期413-423,共11页
对生活污水的前处理进行优化,建立了一种在线固相萃取-超高效液相色谱-串联质谱法(Online-SPE-UPLC-MS/MS)同时测定污水中20种毒品及人口标记物的方法。用金属螯合剂Na_(2)EDTA·2H_(2)O处理污水后加入同位素内标,接着用滤膜过滤后... 对生活污水的前处理进行优化,建立了一种在线固相萃取-超高效液相色谱-串联质谱法(Online-SPE-UPLC-MS/MS)同时测定污水中20种毒品及人口标记物的方法。用金属螯合剂Na_(2)EDTA·2H_(2)O处理污水后加入同位素内标,接着用滤膜过滤后,以双柱交替模式上样。用Waters Oasis HLB固相萃取柱(2.1 mm×30 mm,20μm)进行富集浓缩后,用流动相将固相萃取柱上吸附的目标物反冲洗到Waters ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm)上。柱温40℃,以乙腈-0.1%(体积分数)甲酸水溶液为流动相,采用梯度程序洗脱。采用电喷雾离子源正离子、多反应监测(MRM)模式定量检测。可替宁的线性范围在20~5000 ng·L^(-1),其余20种物质的线性范围在1~250 ng·L^(-1)之间,相关系数R2均大于0.99,检出限在0.01~0.25 ng·L^(-1)之间,加标回收率在91.57%~113.94%,精密度均小于15%。基于Online-SPE-UPLC-MS/MS建立了同时测定污水中20种毒品及人口标记物方法,该方法操作简单、灵敏高,具有良好的准确度和精密度,已成功应用于城市污水中的毒品检测,用于监测毒品滥用情况。 展开更多
关键词 在线固相萃取 双柱交替 超高液相色谱串联质谱 污水 毒品
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