Organic matrix proteins play an important role in the biomineralization of mollusc shells.We have identified and characterized a novel matrix protein,namely hic74,isolated from the mantle of the freshwater mussel Hyri...Organic matrix proteins play an important role in the biomineralization of mollusc shells.We have identified and characterized a novel matrix protein,namely hic74,isolated from the mantle of the freshwater mussel Hyriopsis cumingii.The hic74 gene encodes a 850 amino acid protein that is rich in alanine(ala,30.8%),glycine(gly,25.8%)and serine(ser,10.6%),with ala mainly existing in poly-ala forms within the ala/gly-rich regions.Quantitative PCR expression analysis demonstrated that hic74 was specifically amplified from the mantle and in situ hybridization showed a strong signal in the epithelial cells in the pallial region of the mantle.Lower levels of expression were detected during the early stages of pearl sac formation but increased and remained constant during nacreous layer formation suggesting that hic74 might be involved in this process.展开更多
UroporphyrinogenⅢsynthetase(UROS)is an important enzyme involved in the structural formation of porphyrin,which is responsible for color intensity.A UROS homolog was isolated and characterized from the pearl mussel,H...UroporphyrinogenⅢsynthetase(UROS)is an important enzyme involved in the structural formation of porphyrin,which is responsible for color intensity.A UROS homolog was isolated and characterized from the pearl mussel,Hyriopsis cumingii(HcUros).The HcUros cDNA was 858 bp in length with an open reading frame that encoded a 285 amino acid protein,which contained a conserved HemD domain.HcUros exhibited widespread tissue distribution,displaying particular enrichment in the mantle and haemolymph of purple mussels,compared to white mussels.In situ hybridization of HcUros in mantle tissue demonstrated that it was specifically expressed in the dorsal epithelial cells of the mantle pallia.Additionally,HcUros expression was upregulated following implantation of the pearl sac to produce pearls.The data suggest that HcUros contributes to shell pigmentation,and nacre formation and coloring in H.cumingii.展开更多
基金This work was supported by the National Science and Technology Support Program(2012BAD26B04)the National Natural Science Foundation of China(31272657)Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding(ZF1206).
文摘Organic matrix proteins play an important role in the biomineralization of mollusc shells.We have identified and characterized a novel matrix protein,namely hic74,isolated from the mantle of the freshwater mussel Hyriopsis cumingii.The hic74 gene encodes a 850 amino acid protein that is rich in alanine(ala,30.8%),glycine(gly,25.8%)and serine(ser,10.6%),with ala mainly existing in poly-ala forms within the ala/gly-rich regions.Quantitative PCR expression analysis demonstrated that hic74 was specifically amplified from the mantle and in situ hybridization showed a strong signal in the epithelial cells in the pallial region of the mantle.Lower levels of expression were detected during the early stages of pearl sac formation but increased and remained constant during nacreous layer formation suggesting that hic74 might be involved in this process.
基金the National Key R&D Program of China(2018YFD0901406)the National Natural Science Foundation of China(31872565)sponsored by the Program of the Shanghai Academic Research Leader(19XD1421500).
文摘UroporphyrinogenⅢsynthetase(UROS)is an important enzyme involved in the structural formation of porphyrin,which is responsible for color intensity.A UROS homolog was isolated and characterized from the pearl mussel,Hyriopsis cumingii(HcUros).The HcUros cDNA was 858 bp in length with an open reading frame that encoded a 285 amino acid protein,which contained a conserved HemD domain.HcUros exhibited widespread tissue distribution,displaying particular enrichment in the mantle and haemolymph of purple mussels,compared to white mussels.In situ hybridization of HcUros in mantle tissue demonstrated that it was specifically expressed in the dorsal epithelial cells of the mantle pallia.Additionally,HcUros expression was upregulated following implantation of the pearl sac to produce pearls.The data suggest that HcUros contributes to shell pigmentation,and nacre formation and coloring in H.cumingii.
