Plant growth promoting fungi are receiving increased attention as valuable beneficial microorganisms in crop cultivation due to their capacity to produce bioactive substances,promote plant growth and enhance immune de...Plant growth promoting fungi are receiving increased attention as valuable beneficial microorganisms in crop cultivation due to their capacity to produce bioactive substances,promote plant growth and enhance immune defense functions.In this study,a novel Trichoderma isolate,designated as TM2-4,was screened from healthy tomato rhizosphere soil and identified as Trichoderma afroharzianum.Culture filtrate of the isolate TM2-4 displayed obvious bioactive substance production and an evident effect in promoting tomato seed germination,with hypocotyl length,radical length and vigor index increased by 28.7,19.4 and 62.1%,respectively,after a 100-fold dilution treatment.To assess the promotion effect and related mechanism of isolate TM2-4,the plant biological indexes and gene expression profiles of tomato plants treated with or without T.afroharzianum TM2-4 microbial agent were investigated by greenhouse pot experiment and RNA sequencing.The results demonstrated that T.afroharzianum TM2-4 significantly promoted tomato plant growth in terms of plant height,dry weight,number of leaves per plant and root activity,through efficient colonization in the rhizosphere and root system of the plants.Transcriptome analyses identified a total of 984 differentially expressed genes in T.afroharzianum microbial agent inoculated tomato roots,which were mainly engaged in the biological process of phytohormone homeostasis,antioxidant activity,as well as metabolic pathways including phenylpropanoid biosynthesis and glutathione metabolism.These findings provide useful information for understanding the mechanism of isolate TM2-4 for tomato plant growth promotion,which would facilitate further development of T.afroharzianum TM2-4 microbial agent for use in vegetable crop production.展开更多
采用THSM选择性培养基,从西藏、山东和云南等地的土壤中分离获得木霉菌株Z19、CX58-4和CY358-5。通过形态学特征、ITS r DNA和TEF-1α序列对菌株进行鉴定。结果表明,3个菌株属于哈茨木霉(Trichoderma harzianum)复合种,分别是非洲哈茨木...采用THSM选择性培养基,从西藏、山东和云南等地的土壤中分离获得木霉菌株Z19、CX58-4和CY358-5。通过形态学特征、ITS r DNA和TEF-1α序列对菌株进行鉴定。结果表明,3个菌株属于哈茨木霉(Trichoderma harzianum)复合种,分别是非洲哈茨木霉(T.afroharzianum)、深褐木霉(T.atrobrunneum)和西蒙斯木霉(T.simmonsii),均为木霉属中国新记录种。展开更多
选取分离自河北安国中药材种植基地不同药用植物根区土壤的7株木霉菌,通过羧甲基纤维素钠培养基初筛及玉米秸秆、甘草药渣固态发酵培养基复筛,联合筛选纤维素降解木霉菌株,采用形态学和分子生物学相结合方法进行菌株鉴定,并对其生物学...选取分离自河北安国中药材种植基地不同药用植物根区土壤的7株木霉菌,通过羧甲基纤维素钠培养基初筛及玉米秸秆、甘草药渣固态发酵培养基复筛,联合筛选纤维素降解木霉菌株,采用形态学和分子生物学相结合方法进行菌株鉴定,并对其生物学特性进行探究.结果表明,经羧甲基纤维素钠培养基初筛,有4株菌表现出纤维素降解能力,分别为HQ1、BB1、DS3和DS1.经2种木质纤维素底物固态发酵培养基复筛发现,BB1以玉米秸秆为发酵基质时滤纸纤维素酶(filter paper cellulase,FPase)活性最高,HQ1以甘草药渣为基质时FPase活性最高.结合菌落形态、显微结构和DNA分子鉴定,HQ1被鉴定为长枝木霉(Trichoderma longibrachiatum),BB1为非洲哈茨木霉(Trichoderma afroharzianum).2株菌生物学特性存在趋同性,适宜生长产孢pH为5~6,适宜培养温度为28~33℃,且都表现出抵御干旱胁迫的能力.变差分解表明,不同培养条件对木霉菌生长速率及产孢有重要影响.本实验可为后续进一步优化HQ1和BB1降解不同木质纤维素底物固态发酵培养条件提供依据.展开更多
基金the Youth Research Fund of Beijing Academy of Agriculture and Forestry Sciences,China(QNJJ201814)the National Key R&D Program of China(2017YFD0201102)the Beijing Key Laboratory of Green Control of Fruit Tree Diseases and Pests in the North China(BZ0432)。
文摘Plant growth promoting fungi are receiving increased attention as valuable beneficial microorganisms in crop cultivation due to their capacity to produce bioactive substances,promote plant growth and enhance immune defense functions.In this study,a novel Trichoderma isolate,designated as TM2-4,was screened from healthy tomato rhizosphere soil and identified as Trichoderma afroharzianum.Culture filtrate of the isolate TM2-4 displayed obvious bioactive substance production and an evident effect in promoting tomato seed germination,with hypocotyl length,radical length and vigor index increased by 28.7,19.4 and 62.1%,respectively,after a 100-fold dilution treatment.To assess the promotion effect and related mechanism of isolate TM2-4,the plant biological indexes and gene expression profiles of tomato plants treated with or without T.afroharzianum TM2-4 microbial agent were investigated by greenhouse pot experiment and RNA sequencing.The results demonstrated that T.afroharzianum TM2-4 significantly promoted tomato plant growth in terms of plant height,dry weight,number of leaves per plant and root activity,through efficient colonization in the rhizosphere and root system of the plants.Transcriptome analyses identified a total of 984 differentially expressed genes in T.afroharzianum microbial agent inoculated tomato roots,which were mainly engaged in the biological process of phytohormone homeostasis,antioxidant activity,as well as metabolic pathways including phenylpropanoid biosynthesis and glutathione metabolism.These findings provide useful information for understanding the mechanism of isolate TM2-4 for tomato plant growth promotion,which would facilitate further development of T.afroharzianum TM2-4 microbial agent for use in vegetable crop production.
文摘采用THSM选择性培养基,从西藏、山东和云南等地的土壤中分离获得木霉菌株Z19、CX58-4和CY358-5。通过形态学特征、ITS r DNA和TEF-1α序列对菌株进行鉴定。结果表明,3个菌株属于哈茨木霉(Trichoderma harzianum)复合种,分别是非洲哈茨木霉(T.afroharzianum)、深褐木霉(T.atrobrunneum)和西蒙斯木霉(T.simmonsii),均为木霉属中国新记录种。
文摘选取分离自河北安国中药材种植基地不同药用植物根区土壤的7株木霉菌,通过羧甲基纤维素钠培养基初筛及玉米秸秆、甘草药渣固态发酵培养基复筛,联合筛选纤维素降解木霉菌株,采用形态学和分子生物学相结合方法进行菌株鉴定,并对其生物学特性进行探究.结果表明,经羧甲基纤维素钠培养基初筛,有4株菌表现出纤维素降解能力,分别为HQ1、BB1、DS3和DS1.经2种木质纤维素底物固态发酵培养基复筛发现,BB1以玉米秸秆为发酵基质时滤纸纤维素酶(filter paper cellulase,FPase)活性最高,HQ1以甘草药渣为基质时FPase活性最高.结合菌落形态、显微结构和DNA分子鉴定,HQ1被鉴定为长枝木霉(Trichoderma longibrachiatum),BB1为非洲哈茨木霉(Trichoderma afroharzianum).2株菌生物学特性存在趋同性,适宜生长产孢pH为5~6,适宜培养温度为28~33℃,且都表现出抵御干旱胁迫的能力.变差分解表明,不同培养条件对木霉菌生长速率及产孢有重要影响.本实验可为后续进一步优化HQ1和BB1降解不同木质纤维素底物固态发酵培养条件提供依据.