The potential of several endophytic Trichoderma isolates, isolated from healthy oil palm roots was assessed against Ganoderma boninense (PER71), the causal agent of Basal Stem Rot (BSR) disease through in vitro screen...The potential of several endophytic Trichoderma isolates, isolated from healthy oil palm roots was assessed against Ganoderma boninense (PER71), the causal agent of Basal Stem Rot (BSR) disease through in vitro screening. In the dual culture assay, all endophytic Trichoderma isolates were found to be capable of inhibiting the growth of PER71 mycelium, by causing more than 70% inhibition of the pathogen’s radial growth (PIRG). Trichoderma asperellum M103 has caused the highest inhibition at 93.14%. The volatile compound produced by M103 also suppressed 65% of the pathogen’s radial growth. On the other hand, T. harzianum, M108 was found to have the ability to produce non-volatile compounds that is toxic and it has effectively inhibited 98.18% of the growth of PER71. Collectively, T. asperellum M103 and T. harzianum M108 have the potential to inhibit the growth of G. boninense PER71. These isolates were shown to be viable options for the future management of G. boninense in the oil palm field.展开更多
From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized...From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D ( 14−17 ) were also identified. The structural configurations of the newly identified peptaibols ( 1−13 ) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey’s method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols ( 1 − 7 , 14 − 17 ) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8−32 μg·mL^(−1). Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL^(−1).展开更多
文摘The potential of several endophytic Trichoderma isolates, isolated from healthy oil palm roots was assessed against Ganoderma boninense (PER71), the causal agent of Basal Stem Rot (BSR) disease through in vitro screening. In the dual culture assay, all endophytic Trichoderma isolates were found to be capable of inhibiting the growth of PER71 mycelium, by causing more than 70% inhibition of the pathogen’s radial growth (PIRG). Trichoderma asperellum M103 has caused the highest inhibition at 93.14%. The volatile compound produced by M103 also suppressed 65% of the pathogen’s radial growth. On the other hand, T. harzianum, M108 was found to have the ability to produce non-volatile compounds that is toxic and it has effectively inhibited 98.18% of the growth of PER71. Collectively, T. asperellum M103 and T. harzianum M108 have the potential to inhibit the growth of G. boninense PER71. These isolates were shown to be viable options for the future management of G. boninense in the oil palm field.
基金supported by the National Key Research and Development Program of China(No.2018YFA0903200//2018YFA0903201)the National Natural Science Foundation of China(Nos.81925037 and 81973213)+6 种基金National High-level Personnel of Special Support Program(No.2017RA2259)the 111 Project of Ministry of Education of the People's Republic of China(No.B13038)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036)Guangdong International Science and Technology Cooperation Base(No.2021A0505020015)Innovative and Research Teams Project of Guangdong Higher Education Institution(No.2021KCXTD001)K.C.Wong Education Foundation(No.Guo-Dong Chen,2021,China)the Science and Technology Planning Project of Guangzhou(No.202201020048)。
文摘From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D ( 14−17 ) were also identified. The structural configurations of the newly identified peptaibols ( 1−13 ) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey’s method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols ( 1 − 7 , 14 − 17 ) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8−32 μg·mL^(−1). Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL^(−1).
