Effects of Seaweed Bio-organic Fertilizer on Growth and Yield of Winter Wheat

[Objective] This study aimed to investigate the effects of seaweed bio-or- ganic fertilizer on yield and quality of winter wheat. [Method] Seaweed bio-organic fertilizer was applied to leaves of winter wheat according to the dose of 45 kg/hm^2 from jointing stage to maturing stage, and plant height, dry matter accumulation, flag leaf photosynthetic characteristics and grain yield of winter wheat were investigated. [Result] Foliar spraying of seaweed bio-organic fertilizer showed little effect on plant height of winter wheat, thickened stems, promoted dry matter accumulation, in- creased flag leaf photosynthetic rate by 3.16%, and increased yield of winter wheat by 6.85%. [Conclusion] Foliar spraying of seaweed bio-organic fertilizer promoted the intelligent growth, thickened the stems, improved the lodging resistance, significantly increased the panicle weight per plant, and increased the bulk density of winter wheat, as well as improving the physical quality of wheat grain. In addition, foliar spraying of seaweed bio-organic fertilizer promoted the synthesis of chlorophyll and mitigated the decomposition of chlorophyll in winter wheat. Under the background of fertilizer-pesticide double reduction, the test results and data of this study can be promoted in the wheat-growing areas of Shandong Province and even whole China.

Characters of Bio-organic Fertilizer and Trails on Its Effect When Applied to Peach

[Objective] This study aimed to investigate the characteristics of bio-organic fertilizer and its effect when applied to peach.[Method] Through launching demonstration trial on the application of bio-organic fertilizer in the major fruit producing areas in Liaoning Province,the effects of bio-organic fertilizer on peach growth and soil were investigated.[Results] After application of bio-organic fertilizer,both the peach yield and fruit quality were improved to some extent,of which yields was increased by 16.4％ compared with the control,and vitamin C and total sugar contents were also significantly increased; application of bio-organic fertilizer also improved the contents of total nitrogen,rapidly available phosphorus,available potassium and organic matter in soil,and reduced the soil volume weight.[Conclusion] Bioorganic fertilizer can significantly improve fruit yield and quality,as well as improving orchard soil and protecting the environment,thus possessing a bright application prospect in the production of green fruits.

Effects of Liquid Seaweed Bio-organic Fertilizer on Seed Germination and Seedling Growth of Vegetables

[Objective] This study aimed to investigate the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of different vegetables. [Method] Serial dilution concentrations of liquid seaweed bio-organic fertilizer were prepared for seed soaking and pot incubation of cucumber, tomato and chili, to ob- serve the effects of liquid seaweed bio-organic fertilizer on seed germination and seedling growth of vegetables. [Result] Compared with the control, germination rate of cucumber and tomato seeds applied with 600-fold liquid seaweed bio-organic fer- tilizer varied significantly; germination rate of chili seeds applied with 400-fold liquid seaweed bio-organic fertilizer varied significantly; germination energy and germination index of chili seeds applied with different dilution concentrations of liquid seaweed bio-organic fertilizer presented no significant differences. In addition, 200-fold and 400-fold liquid seaweed bio-organic fertilizer significantly improved the root length, plant height, plant fresh weight, plant dry weight, chlorophyll content and leaf area of cucumber, tomato and chili seedlings; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content and leaf area of cucumber seedlings varied significantly compared with the control, but no significant differences were observed in plant height, plant fresh weight and plant dry weight; after treated with 600-fold liquid seaweed bio-organic fertilizer, root length, chlorophyll content, plant height, plant fresh weight and plant dry weight of chili and tomato varied sig- nificantly compared with the control, but no significant differences were observed in leaf area. [Conclusion] Soaking vegetable seeds with liquid seaweed bio-organic fer- tilizer can significantly improve seed generation rate and seedling growth.

Effects of Bio-organic Fertilizer with Antagonistic Bacteria against Tobacco Bacterial Wilt on Soil Microbial Communities and Disease Resistance of Tobacco

In this study, the effects of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt on soil microbial communities and disease resist- ance of tobacco were investigated by field experiment. The results showed that the incidence of tobacco bacterial wilt in bio-organic fertilizer treatments （T3 and T4） decreased remarkably among four treatments in the field. Compared with the local conventional fertilization group, the incidence of tobacco bacterial wilt was re- duced by 21.9% and 25.0% in T3 and T4, respectively ; the yield of flue-cured tobacco was improved by 5.7% and 5.3%, respectively ; the proportion of mid- high grade tobacco leaves increased by 2.3% and 2.6%, respectively. After application of bio-organie fertilizer with antagonistic bacteria against tobacco bacterial wilt, rhizosphere soil microbial communities exhibited vast amount and abundant species ; the amount of rhizosphere soil bacteria of infected tobacco plants was im- proved by 218.5% with fewer species. It could be concluded that the application of bio-organic fertilizer with antagonistic bacteria against tobacco bacterial wilt could improve the ecological environment of tobacco field, inhibit the growth of pathogenic bacteria, decrease the incidence of tobacco bacterial wilt, and enhance the quality of flue-cured tobacco. This study laid the foundation for further ecological prevention and control of soil-borne diseases of tobacco.

Effects of Partial Replacement of Chemical Fertilizer by Bio-organic Fertilizer Kunyijian on Yield and Quality of Tobacco Leaves

To explore the application effect of bio-organic fertilizer Kunyijian in flue-cured tobacco and provide scientific basis for its application in production,a plot trial was conducted with K326,a flue-cured tobacco variety,in the Science and Technology Test Base of Xundian County,Yunnan.Taking local fertilizer consumption(control 1)and 70%local fertilizer consumption(control 2)as controls,the following replacement groups were designed:under uniformly replacement 70%local fertilizer consumption,(i)organic fertilizer∶microbial agent=80∶1(kg),+600 kg/ha,+900 kg/ha,+1200 kg/ha,+1500 kg/ha Kunyijian;(ii)organic fertilizer∶microbial agent=40∶1(kg),+900 kg/ha Kunyijian.The results show that in the treatment of 70%local fertilizer consumption+1200 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)],the comprehensive performance of flue-cured tobacco was significantly better than that in the control groups,the yield of tobacco leaves reached 2237.1 kg/ha,the output value was 45505.2 yuan/ha,and the average price of the tobacco leaves was 20.53 yuan/kg.The performance of flue-cured tobacco in the treatment of 70%local fertilizer consumption+1500 kg/ha Kunyijian ranked second.The performance of other fertilization treatments was not good,but it did not differ significantly from that of control 1.In terms of chemical composition of tobacco leaves,partial replacement of chemical fertilizer by bio-organic fertilizer Kunyijian,was beneficial to increase the sugar content and reduce the nicotine and total nitrogen contents in tobacco leaves,especially the treatment of 70%local fertilizer consumption+900 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)],and it was also beneficial to increase the chlorine content in tobacco leaves,especially the treatment of 70%local fertilizer consumption+1500 kg/ha Kunyijian[organic fertilizer∶microbial agent=80∶1(kg)].Therefore,it is feasible to use the bio-organic fertilizer Kunyijian to replace 30%of chemical fertilizer in tobacco production.The rate is recommended to be 900-1500 kg/ha[organic fertilizer∶microbial agent=80∶1(kg)].It is suggested to further strengthen the demonstration and promotion of Kunyijian.

Application of Bio-organic Fertilizer Containing High-efficient Nitrogen-fixing Bacillus amyloliquefaciens on Strawberry

With strawberry as a test material,the effects of the bio-organic fertilizer containing high-efficiency nitrogen-fixing Bacillus amyloliquefaciens on nutrient contents in strawberry-planted soil,wilt occurrence and strawberry yield and quality were studied by a plot experiment,so as to provide reference for scientific use of the bio-organic fertilizer and green production of strawberry.The results showed that after hole-applying the bio-organic fertilizer at a rate of 22.5 t/hm^2,the contents of NH^+_4-N,available P,available K and organic matter did not change much with time;and when replacing 50%of chemical fertilizers with the bio-organic fertilizer at a rate of 11.25 t/hm^2(K_3),the contents of NH^+_4-N and available P in the soil did not change much with time,and the contents of available K and organic matter decreased slightly with time,but were both higher than the CK(the unfertilized treatment).Meanwhile,the disease index values of strawberry wilt disease in treatments K_2 and K_3were significantly lower than those of the CK and the conventional fertilization treatment(K_1),and the vitamin C contents of strawberry fruit in the two treatments were significantly higher than that of the CK.The yield determination showed that the cumulative yields of treatments K_2 and K_3 increased by 9.8% and 3.3%,respectively,and the increase rates of the early yields(before the Spring Festival)were 30.6% and 21.9%,respectively.Therefore,the application of the bio-organic fertilizer can replace chemical fertilizers,and can achieve the effects of reducing the occurrence of wilt,improving the early yield of fruit commodity and improving fruit quality.

Application Effect of Bio-organic Fertilizer on Chuanzhu 16

Through the application of bio-organic fertilizer on Chuanzhuo 16, the re- sults showed that the yield could reach 185.23 kg in treatment with bio-organic fer- tilizer, 40.22 kg more than the conventional fertilization, and the yield increase rate reached up to 27.7%.

Effects of Full-Element Bio-Organic Fertilizer on Growth,Yield,and Rhizosphere Soil Nutrients of Pepper

A field experiment was conducted to evaluate the effects of self-developed full-element bio-organic fertilizer on the growth,yield,and rhizosphere soil nutrients of pepper.Four treatments were designed,including full-element bio-organic fertilizer+conventional fertilizer reduced by 50%(T1),inactivated full-element bio-organic fertilizer+conventional fertilizer reduced by 50%(T2),conventional fertilizer(T3),and no fertilizer(CK).The results showed that T1 significantly increased the plant height,crown width,fruit number per plant,and yield of pepper.T1 had higher pH value,total nitrogen,total phosphorus,total potassium,available nitrogen,available phosphorus,and available potassium in the rhizosphere soil than T3 and CK,and it had higher available phosphorus and available potassium than T2.The disease index of bacterial wilt in T1 was 21.74,which was 10.37,20.19,and 35.48 lower than T2,T3,and CK,respectively.The control effect of T1 reached 56.71%.The above results indicated that whole bio-organic fertilizer promoted the growth to improve the yield and benefit of pepper.Moreover,the fertilizer activated soil nutrients to improve soil fertility and reduced soil-borne diseases.Therefore,the full-element bio-organic fertilizer can be promoted in the pepper fields with continuous cropping obstacles.

Effects of Bioorganic Fertilizers on the Leaf Antioxidant Enzyme Activity and the Photosynthetic Characteristics of Replanted Szechuan Pepper, Zanthoxylum simulans

Continuous cropping obstacles hamper the efficient growth and yield of Szechuan pepper,Zanthoxylum simulans.The current study investigated the impact of different levels of bioorganic fertilizer on the leaf physiological and photosynthetic characteristics of Z.simulans to provide a theoretical reference for continuous Z.simulans crop cultivation.A bioorganic fertilizer was used to treat seedlings growing in 25-year-old continuous cropping soil.Five fertilizer treatments were applied.The impacts of the treatments on the activity of defense enzyme and photosynthetic parameters of Z.simulans leaves were determined.The different concentrations of bioorganic fertilizer reduced to varying degrees the malondialdehyde(MDA)content and intercellular CO2 concentration(Ci),and increased the activity of peroxidase(POD),superoxide dismutase(SOD),and ascorbate peroxidase(APX),as well as the chlorophyll content,net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of Z.simulans leaves.The results showed that most significant increases or decreases were achieved with 100 g/L bioorganic fertilizer(Y2).Thus,the application of bioorganic fertilizer at a rate of 100 g/L can significantly improve the activity of relevant defense enzymes and photosynthetic parameters of Z.simulans,and reduce the MDA content,enhancing the stress resistance of the plants,promoting their growth and addressing,to some extent,obstacles associated with continuous cultivation.

Effects of <i>Bacillus cereus</i>F-6 on Promoting Vanilla (<i>Vanilla planifolia</i>Andrews.) Plant Growth and Controlling Stem and Root Rot Disease

A lipopeptide-producing bacterium, Bacillus cereus (F-6), was isolated from the rhizosphere soil of a healthy vanilla (Vanilla planifolia) plant cultivated on a plantation under 21 years of continuous cropping with vanilla. A pot experiment was conducted to investigate the effects of the green fluorescent protein-tagged F-6 (F-6-gfp) and its bio-organic fertilizer (BIO) on vanilla plant growth and stem and root rot disease, using the same plantation soil. The application of BIO significantly increased the vanilla plant root, stem and leave dry weights;however, there was not a significant difference between the F-6-gfp-inoculated treatment and the control. Meanwhile, the BIO application also significantly reduced the severity of stem and root rot disease compared to the control. The rhizosphere soil population of Fusarium was approximately 10-fold smaller in the BIO treatment compared to the control treatment at 150 days after transplantation. The number of B. cereus F-6-gfp in the rhizosphere soil of the BIO treatment remained significantly higher than that of the F-6-gfp-inoculated treatment throughout the experiment. In conclusion, F-6-gfp successfully colonized the rhizosphere soil in the BIO treatment, promoting vanilla plant growth, reducing the disease severity index, and decreasing the Fusarium population number, helping to remove barriers to the continuous cropping of vanilla.

Bacillus amyloliquefaciens Strain W19 can Promote Growth and Yield and Suppress Fusarium Wilt in Banana Under Greenhouse and Field Conditions

Plant growth-promoting rhizobacteria （PGPR） are considered to be the most promising agents for cash crop production via increasing crop yields and decreasing disease occurrence. The Bacillus amyloliquefaciens strain W19 can produce secondary metabolites （iturin and bacillomycin D） effectively against Fusarium oxysporum f. sp. cubense （FOC）. In this study, the ability of a bio-organic fertilizer （BIO） containing strain W19 to promote plant growth and suppress the Fusarium wilt of banana was evaluated in both pot and field experiments. The results showed that application of BIO significantly promoted the growth and fruit yield of banana while suppressing the banana Fusariurn wilt disease. To further determine the beneficial mechanisms of the strain, the colonization of green fluorescent protein-tagged strain W19 on banana roots was observed using confocal laser scanning microscopy and scanning electron microscopy. The effect of banana root exudates on the formation of biofilm of strain W19 indicated that the banana root exudates may enhance colonization. In addition, the strain W19 was able to produce indole-3-acetic acid （IAA）, a plant growth-promoting hormone. The results of these experiments revealed that the application of strain W19-enriched BIO improved the banana root colonization of strain W19 and growth of banana and suppressed the Fusarium wilt. The PGPR strain W19 can be a useful biocontrol agent for the production of banana under field conditions.