Extraction and Refining Process of Polysaccharide from Tricholoma matsutake and Verification of Its Whitening Effect

[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of eluent concentration,loading amount and diameter-to-height ratio on the refining process of T.matsutake polysaccharide were explored by orthogonal test,to optimize the dynamic elution conditions of T.matsutake polysaccharide.The survival rate of mouse melanoma cells(B16),inhibition of melanin synthesis and inhibition of tyrosinase activity of crude extract A1 and refined extracts A2-A4 were tested to verify the whitening effect of T.matsutake.[Results]The best extraction and refining process for T.matsutake polysaccharide was:eluent 40%ethanol(volume fraction),loading amount 30 mg/g(polysaccharide-resin),diameter-to-height ratio 1∶8.Under this condition,the extraction rate of T.matsutake can reach up to 33%(A3).The whitening efficacy test showed that crude extract A1 and refined extracts A2-A4 had good whitening effects,and the whitening effects were A3≥A4>A1>A2.[Conclusions]The study provides a reference for the application of T.matsutake polysaccharide in cosmetics.

Preliminary Analysis of Genetic Characteristics of Edible Fungus Tricholoma matsutake Isolated from Yajiang, China, by AFLP Technique

A total of 10 Tricholoma matsutake strains isolated from Yajiang County, Sichuan Province of China were analyzed by using AFLP technique. The results showed that the genetic characteristics of Tricholoma matsutake strains can be properly revealed by AFLP, and among the 10 Tricholoma matsutake strains, the genetic aspects were very similar. At the level of 90% similarity, all the strains collected together, and at the similarity of 92 % , three AFLP groups formed. Simultaneously, the effects of quantity of the primers on the second AFLP PCR were determined. The optimal quantity of Eco +1/Mse+1, Eco + 2/Mse + 2 and Eco + 3/ Mse+3 primer pairs used in the AFLP was 1 pmol/5 pmol, 0. 6 pmol/10 pmol and 0.1 pmol/8 pmol, respectively.

Conservation and Development of Forest Mushrooms: A Case Study on Song Rong (Tricholoma matsutake) from China

Song Rong (i.e. Matsutake, Tricholoma matsutake) is the most favorite edible mushroom in Japan and restrictedly distributes in forests in East Asia. The yield of Song Rong, however, has been extremely dropping since 1940 's in the region due to changes of environmental ecology of nature forests. As a compensation for consumption, Japan imported products of T. matsutake from Korea and China, and T. magnivelare from North America since mid-1970s, but T. matsutake stili accounts for a large portion of the ...

Climate change effects fruiting of the prize matsutake mushroom in China

Climate change affects various facets of life but there is little data on its effects on wild mushroom fruiting.Yunnan Province in China is a rich source of wild mushrooms and has experienced a temperature rise over recent decades.This has resulted in warmer temperatures but the impacts of these changes on mushroom production lack documentation.We collected data on the fruiting of the highly prized matsutake mushroom(Tricholoma matsutake)in West Yunnan,China over an 11 year period from 2000 to 2010.Fruiting phenology and productivity were compared against the driving meteorological variables using Projection to Latent Structure regression.The mushrooms appeared later in the season during the observation period,which is most likely explained by rising temperatures and reduced rain during May and June.High temperature and abundant rain in August resulted in good productivity.The climate response of matsutake production results from a sequence of processes that are possibly linked with regulatory signals and resource availability.To advance the knowledge of this complex system,a holistic research approach integrating biology,ecology,genetics,physiology,and phytochemistry is needed.Our results contribute to a general model of fungal ecology,which can be used to predict the responses of fungi to global climate change.