Twelve steroids, including eight ergostane-type sterols and four mono-glucosides of ergostane-type sterols, were isolated from the ethyl acetate soluble fraction of the fungus Pleurotus ostreatus ( Jacq. : Fr.) Kummer...Twelve steroids, including eight ergostane-type sterols and four mono-glucosides of ergostane-type sterols, were isolated from the ethyl acetate soluble fraction of the fungus Pleurotus ostreatus ( Jacq. : Fr.) Kummer. A new compound 3-O-beta-D-glucopyranosyl-22E, 24R-ergosta-7, 22-diene-5alpha, 6beta, 9alpha-triol, was structurally elucidated by spectroscopic and chemical methods. The eleven known compounds were 22E, 24R-ergosta-7, 22-diene-3beta, 5alpha, 6beta, 9alpha-tetraol; 3-O-beta-D-glucopyranosyl-22E, 24R-ergosta-7, 22-diene-5alpha, 6beta-diol; 22E, 24R-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol; 22E, 24R-ergosta-5, 7, 22-trien-3beta-ol 3-O-beta-D-glucopyranoside; ergosterol; 22E, 24R-ergosta-7, 22-dien-3beta-ol 3-O-beta-D-glucopyranoside; 22E, 24R-ergosta-7, 22-diene-3beta-ol; 22E, 24R-ergosta-4, 6, 8, 22-tetraen-3-one; 22E, 24R-ergosta-7, 22-diene-3beta, 5alpha, 6alpha-triol; ergosterol peroxide; 22E, 24R-ergosta-7, 22-diene-3beta, 5alpha-diol-6-one.展开更多
Arrhenia pontevedrana is described as a new species to science from Galicia(NW of Iberian Peninsula)based on morphological characteristics.A detailed description of basidiomata and microstructures of this species and ...Arrhenia pontevedrana is described as a new species to science from Galicia(NW of Iberian Peninsula)based on morphological characteristics.A detailed description of basidiomata and microstructures of this species and a comparison with its morphologically closely related taxa are given.Arrhenia subglobisemen is recorded for the first time from the Iberian Peninsula.The new combination Arrhenia kuhnerii is also proposed.展开更多
Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chroma...Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chromatography and their structures were elucidated on the basis of the 1D and 2D NMR spectroscopic and HRESIMS data.Cytotoxicity of all isolates against A549,HCT-116,M231 and W256 human tumor cells was determined by the MTT method.Results:A new sesquiterpene aryl ester,armimelleolide C(1),and eight known ones including armillarivin(2),melleolide F(3),6'-chloromelleolide F(4),melleolide(5),melleolide K(6),melledonol(7),13-hydroxydihydromelleolide(8),and armillane(9),were isolated from the EtOAc extract of A.gallica 012m.All isolates showed potential cytotoxic activities against at least one of the human cancer cell lines with IC_(50) values ranging from(3.17±0.54)to(17.57±0.47)μmol/L.Compound 1 showed significant inhibitory activity against M231 with an IC_(50) value of(7.54±0.24)μmol/L compared with paclitaxel as the positive control.Compounds 2,3,and 7,9 showed obvious inhibitory activity against HCT-116 and were better than that of the positive control.Conclusion:The chemical constituents including a new sesquiterpene aryl ester armimelleolide C(1)from the EtOAc extract of A.gallica 012m have a variety of structures and potential antitumor activities.展开更多
文摘Arrhenia pontevedrana is described as a new species to science from Galicia(NW of Iberian Peninsula)based on morphological characteristics.A detailed description of basidiomata and microstructures of this species and a comparison with its morphologically closely related taxa are given.Arrhenia subglobisemen is recorded for the first time from the Iberian Peninsula.The new combination Arrhenia kuhnerii is also proposed.
基金This project was in part supported financially by National Natural Science Foundation of China(Nos.31960095 and 82160670)National Natural Science Foundation of Yunnan Province(Nos.202101AS070022 and 202201AT070228)Yunnan Ten thousand Talents Program to Weiguang Wang and Yanping Li.
文摘Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chromatography and their structures were elucidated on the basis of the 1D and 2D NMR spectroscopic and HRESIMS data.Cytotoxicity of all isolates against A549,HCT-116,M231 and W256 human tumor cells was determined by the MTT method.Results:A new sesquiterpene aryl ester,armimelleolide C(1),and eight known ones including armillarivin(2),melleolide F(3),6'-chloromelleolide F(4),melleolide(5),melleolide K(6),melledonol(7),13-hydroxydihydromelleolide(8),and armillane(9),were isolated from the EtOAc extract of A.gallica 012m.All isolates showed potential cytotoxic activities against at least one of the human cancer cell lines with IC_(50) values ranging from(3.17±0.54)to(17.57±0.47)μmol/L.Compound 1 showed significant inhibitory activity against M231 with an IC_(50) value of(7.54±0.24)μmol/L compared with paclitaxel as the positive control.Compounds 2,3,and 7,9 showed obvious inhibitory activity against HCT-116 and were better than that of the positive control.Conclusion:The chemical constituents including a new sesquiterpene aryl ester armimelleolide C(1)from the EtOAc extract of A.gallica 012m have a variety of structures and potential antitumor activities.
