Familial White Piedra Caused by Trichosporon inkin

White piedra is a superficial, chronic, asymptomatic mycoses caused by yeast fungi of the genus Trichosporon. It affects the hair, especially of the head, less frequently of the pubis, perineum, armpit, beard, mustache, eyebrows and eyelashes, and is characterized by the formation of soft nodules or fungal clusters. It affects all age groups and both sexes, predominantly women. Diagnosis is made by direct examination of the affected hair and culture on Sabouraud dextrose agar. The identification of species occurs through more specific identification procedures, such as mass spectrometry (MALDI-TOF) and PCR). The objective of this work is to report two cases of familial white piedra caused by T. inkin identified by PCR.

国内首例皮瘤丝孢酵母真菌血症分离株的表型及rDNA序列鉴定

目的通过对1例"脂膜炎"患者外周血分离株的表型及分子生物学鉴定,报道国内首例皮瘤丝孢酵母菌血症。方法采集患者的血及鼻咽结节组织标本通过真菌培养获得分离株,经沙氏培养基、马铃薯培养基、科玛嘉念珠菌显色培养后形态学观察及API20CAUX系统鉴定,最后经PCR扩增rDNA基因测序证实。采用CLSI制定的M27-A2微量稀释法对病原菌株进行7种药物的体外药敏试验。结果分离株通过表型和分子生物学鉴定为"皮瘤丝孢酵母"。药敏结果显示菌株对两性霉素B耐药,氟康唑、伏立康唑、伊曲康唑、5-氟胞嘧啶敏感。卡泊芬净的抑菌浓度较低,而米卡芬净的抑菌浓度相对较高。患者因多种并发症死亡。结论皮瘤丝孢酵母引起的真菌血症为我国首例报道。API20CAUX酵母鉴定系统可作为鉴定皮瘤丝孢酵母快速而简便的方法。通过分子生物学DNA序列检测分析能鉴定皮瘤丝孢酵母。

皮瘤丝孢酵母所致导尿管相关性尿路感染1例

报道1例皮瘤丝孢酵母引起的导管相关性尿路感染。患者男性,63岁,尿频、尿急、尿痛,伴右侧腰部隐痛。通过对患者的清洁中段尿、留置双J管、肾盂穿刺尿、膀胱尿进行培养及鉴定。在不同培养温度(25℃、35℃)下,沙堡弱培养皿上表现为两种不同形态的菌落。一种是有脑回状褶皱的乳白色圆形菌落;另一种是中间明显凸起的花簇样灰色菌落。哥伦比亚血琼脂培养皿培养96 h以菌落为中心出现三条裂开琼脂的放射状直线。VITEK 2 COMPACT鉴定结果为皮瘤丝孢酵母,确诊患者为皮瘤丝孢酵母引起的导尿管相关性尿路感染。使用氟康唑0.4 g/d静滴1周,患者尿路感染症状基本缓解。