Objective: To study the effects of co-administration of Gynura procumbens (GP) and kelulut honey (KH) on male fertility and libido in diabetes-induced rats. Methods: A total 42 males Sprague Dawley rats aged 8 weeks w...Objective: To study the effects of co-administration of Gynura procumbens (GP) and kelulut honey (KH) on male fertility and libido in diabetes-induced rats. Methods: A total 42 males Sprague Dawley rats aged 8 weeks were randomly and equally divided into six different groups. All groups except a normal control group were induced with 50 mg/kg of streptozotocin (STZ) intravenously to induce diabetes. A positive control group was treated with an antidiabetic drug, metformin (500 mg/kg) whereas a negative control group remained untreated throughout the experiment. Meanwhile, another three treatments on diabetic rat groups were performed and categorised as Group 1 (450 mg/kg GP+ 300 mg/kg KH), Group 2 (450 mg/kg GP + 600 mg/kg KH) and Group 3 (450 mg/kg GP+ 1200 mg/kg KH). Treatments were given for seven consecutive days through oral gavage and all rats were euthanized on day 8th for fasting blood glucose analysis, sperm quality, spermatogenesis, sexual behaviour and implantation sites analysis. Results: Fasting blood glucose levels were significantly reduced after treatment of GP and KH, compared to negative and positive controls. The treated groups showed significant increment in sperm quality compared to all control groups. Testes histology illustrated significant damages on leydig and sertoli cells for both negative and positive controls. On the contrary, co-administration of GP and KH displayed regeneration of leydig and sertoli cells in the testes. Additionally, the number of implantation sites significantly increased in females copulated with treated groups, compared to controls. Besides, the libido analysis displayed improvement of libido in treated groups, compared to all controls. Throughout the study, insignificant variances were recorded between the treated groups, indicating that treatment in Group 1 was sufficient to trigger significant improvement on fasting blood glucose level, fertility, and libido in diabetic male rats. Conclusions: Co-administration of GP and KH has great potential to serve as a pro-fertility agent amongst diabetic patients.展开更多
Dermatitis is a combination of various skin disease agents such as bacteria,fungi,ectoparasites,viruses and metabolic diseases with various clinical symptoms or signs.Dermatitis can cause an imbalance between ROS and ...Dermatitis is a combination of various skin disease agents such as bacteria,fungi,ectoparasites,viruses and metabolic diseases with various clinical symptoms or signs.Dermatitis can cause an imbalance between ROS and antioxidants.Exogenous antioxidants are needed to help balance antioxidant and ROS levels in the body by providing trigona honey.This study aims to determine the MDA and SOD levels of dogs with complex dermatitis after administration of trigona honey.This study used 12 dogs with complex dermatitis,with a moderate severity score.Dogs 4-6 months old.Samples were divided into control group(P0)without honey,fresh honey group(P1)5 mL/head/day and capsule honey group(P2)0.1 mg/head/day.Honey was given for 4 weeks in the treatment group.Examination of MDA and SOD levels was carried out using the ELISA kit.The results showed that the Trigona honey treatment had a significant effect(P<0.05)in reducing MDA levels and increasing SOD levels in dogs with complex dermatitis.Duncan's test results showed that SOD levels increased significantly at week 4,while week 2 and week 0 did not increase significantly.Trigona honey has anti-inflammatory,antibacterial,antiaging,antiulcer effects,helps in wound healing dermatitis and is an antioxidant.So that trigona honey can reduce plasma MDA levels and increase plasma SOD levels in dogs with complex dermatitis.展开更多
文摘Objective: To study the effects of co-administration of Gynura procumbens (GP) and kelulut honey (KH) on male fertility and libido in diabetes-induced rats. Methods: A total 42 males Sprague Dawley rats aged 8 weeks were randomly and equally divided into six different groups. All groups except a normal control group were induced with 50 mg/kg of streptozotocin (STZ) intravenously to induce diabetes. A positive control group was treated with an antidiabetic drug, metformin (500 mg/kg) whereas a negative control group remained untreated throughout the experiment. Meanwhile, another three treatments on diabetic rat groups were performed and categorised as Group 1 (450 mg/kg GP+ 300 mg/kg KH), Group 2 (450 mg/kg GP + 600 mg/kg KH) and Group 3 (450 mg/kg GP+ 1200 mg/kg KH). Treatments were given for seven consecutive days through oral gavage and all rats were euthanized on day 8th for fasting blood glucose analysis, sperm quality, spermatogenesis, sexual behaviour and implantation sites analysis. Results: Fasting blood glucose levels were significantly reduced after treatment of GP and KH, compared to negative and positive controls. The treated groups showed significant increment in sperm quality compared to all control groups. Testes histology illustrated significant damages on leydig and sertoli cells for both negative and positive controls. On the contrary, co-administration of GP and KH displayed regeneration of leydig and sertoli cells in the testes. Additionally, the number of implantation sites significantly increased in females copulated with treated groups, compared to controls. Besides, the libido analysis displayed improvement of libido in treated groups, compared to all controls. Throughout the study, insignificant variances were recorded between the treated groups, indicating that treatment in Group 1 was sufficient to trigger significant improvement on fasting blood glucose level, fertility, and libido in diabetic male rats. Conclusions: Co-administration of GP and KH has great potential to serve as a pro-fertility agent amongst diabetic patients.
文摘Dermatitis is a combination of various skin disease agents such as bacteria,fungi,ectoparasites,viruses and metabolic diseases with various clinical symptoms or signs.Dermatitis can cause an imbalance between ROS and antioxidants.Exogenous antioxidants are needed to help balance antioxidant and ROS levels in the body by providing trigona honey.This study aims to determine the MDA and SOD levels of dogs with complex dermatitis after administration of trigona honey.This study used 12 dogs with complex dermatitis,with a moderate severity score.Dogs 4-6 months old.Samples were divided into control group(P0)without honey,fresh honey group(P1)5 mL/head/day and capsule honey group(P2)0.1 mg/head/day.Honey was given for 4 weeks in the treatment group.Examination of MDA and SOD levels was carried out using the ELISA kit.The results showed that the Trigona honey treatment had a significant effect(P<0.05)in reducing MDA levels and increasing SOD levels in dogs with complex dermatitis.Duncan's test results showed that SOD levels increased significantly at week 4,while week 2 and week 0 did not increase significantly.Trigona honey has anti-inflammatory,antibacterial,antiaging,antiulcer effects,helps in wound healing dermatitis and is an antioxidant.So that trigona honey can reduce plasma MDA levels and increase plasma SOD levels in dogs with complex dermatitis.
