目的探讨基于MRI征象与影像组学诊断进展期胃癌T3和T4a分期效能的对比分析。方法选取于同济大学附属东方医院庐江分院行MRI检查,经病理结果证实,共计纳入208例胃癌患者,其中T3期96例,T4a期112例。首先应用传统影像学征象判断进展期胃癌...目的探讨基于MRI征象与影像组学诊断进展期胃癌T3和T4a分期效能的对比分析。方法选取于同济大学附属东方医院庐江分院行MRI检查,经病理结果证实,共计纳入208例胃癌患者,其中T3期96例,T4a期112例。首先应用传统影像学征象判断进展期胃癌侵犯浆膜层等征象,比较多序列MRI征象在病理证实T3和T4a期胃癌中表现的差异性;其次按7:3的比例随机分为训练组(n=145)和验证组(n=63),分别从常规T2非抑脂序列及高比值DWI序列(b=1000 s/mm^(2))图像中提取影像组学特征,构建影像组学联合模型;然后分别绘制基于传统MRI征象与影像组学联合模型工作特征(receiver operating characteristic,ROC)曲线,并计算ROC曲线下面积(area under the curve,AUC)、特异度及灵敏度,量化两种诊断方式对胃癌T3和T4a分期的诊断效能。结果传统多序列MRI征象诊断AUC:0.929(95%CI:0.887~0.970),特异度0.912,灵敏度0.916;MRI影像组学联合模型训练组诊断AUC:0.975(95%CI:0.974~0.976),特异度0.946,灵敏度0.956,验证组诊断AUC:0.971(95%CI:0.965~0.974),特异度0.946,灵敏度0.943,均具有统计学意义(P<0.05)。结论基于MRI影像组学模型诊断T3和T4a分期效能高于传统MRI征象,值得临床工作中进一步推广使用。展开更多
The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as...The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as selenium and certain vitamins. To assess the thyroid disturbances in the mountain population of Benin, a survey was conducted in Natitingou, a mountain town located in the department of Atacora, in the northwest of Benin, on a sample of thirty (30) adults (15 men and 15 women), most of whom are educated. The results of the questionnaire revealed that 43% of the surveyed population acknowledged having knowledge on the mentioned subject and have dietary habits based on the consumption of seafood, and also legumes (20%). The examination of the serum results of the dosage of T3, T4 and TSH hormones revealed cases of thyroid disturbances in the region (36.32% in men and 44.98% in women). The analysis of a comparative table including the “VEDALAB Easy Reader+” and five (05) other readers, presents the performance, reading techniques, principles, advantages and disadvantages of each device. Pending further studies, some recommendations were made at the end of this study to the academic authorities regarding probable cases of dysthyroidism for which additional examinations are required and an awareness for the improvement of dietary habits.展开更多
Vaccarin,a flavonoid glycoside isolated from Vaccaria segetalis,is non-toxic to 3T3-L1 cells up to concentrations of 200μM.Accordingly,we investigated the effects of this natural product on adipogenesis and lipolysis...Vaccarin,a flavonoid glycoside isolated from Vaccaria segetalis,is non-toxic to 3T3-L1 cells up to concentrations of 200μM.Accordingly,we investigated the effects of this natural product on adipogenesis and lipolysis in 3T3-L1 adipocytes.Our results revealed that vaccarin significantly inhibited lipid accumulation by suppressing the adipogenesis-related transcription factors peroxisome proliferator-activated receptorγ(PPARγ)and the CCAAT/enhancer-binding proteinα(C/EBPα).Specifically,lipid accumulation decreased by up to 27.7±2.7%when 3T3-L1 adipocytes were treated with a 10μM concentration of vaccarin.Mechanistic studies showed that the compound inhibited adipogenesis through activation of the Hedgehog(Hh)signaling pathway and so restoring Smo and Gli1 expression at an early stage of differentiation.In mature 3T3-L1 cells,vaccarin significantly increased the secretion of glycerol into the surrounding medium and thus indicating that it accelerated the degradation of triglycerides.In addition,vaccarin,was shown to enhance lipolysis through stimulation of the transcription levels of lipoprotein lipase,monoglycerides lipase,adipose triacylglyceride lipase,hormone-sensitive lipase and adipose differentiated-related protein.All told,vaccarin suppressed lipid accumulation and enhanced lipolysis during adipocyte differentiation by restoring Hh signaling.As such,it is a phytochemical capable of halting adipocyte hyperplasia and,thereby,ameliorating the effects of obesity.展开更多
文摘目的探讨基于MRI征象与影像组学诊断进展期胃癌T3和T4a分期效能的对比分析。方法选取于同济大学附属东方医院庐江分院行MRI检查,经病理结果证实,共计纳入208例胃癌患者,其中T3期96例,T4a期112例。首先应用传统影像学征象判断进展期胃癌侵犯浆膜层等征象,比较多序列MRI征象在病理证实T3和T4a期胃癌中表现的差异性;其次按7:3的比例随机分为训练组(n=145)和验证组(n=63),分别从常规T2非抑脂序列及高比值DWI序列(b=1000 s/mm^(2))图像中提取影像组学特征,构建影像组学联合模型;然后分别绘制基于传统MRI征象与影像组学联合模型工作特征(receiver operating characteristic,ROC)曲线,并计算ROC曲线下面积(area under the curve,AUC)、特异度及灵敏度,量化两种诊断方式对胃癌T3和T4a分期的诊断效能。结果传统多序列MRI征象诊断AUC:0.929(95%CI:0.887~0.970),特异度0.912,灵敏度0.916;MRI影像组学联合模型训练组诊断AUC:0.975(95%CI:0.974~0.976),特异度0.946,灵敏度0.956,验证组诊断AUC:0.971(95%CI:0.965~0.974),特异度0.946,灵敏度0.943,均具有统计学意义(P<0.05)。结论基于MRI影像组学模型诊断T3和T4a分期效能高于传统MRI征象,值得临床工作中进一步推广使用。
文摘The thyroid, an endocrine gland located at the base of the neck, produces thyroid hormones (triiodothyronine (T3) and thyroxine (T4)). The production of these hormones is possible by iodine and other nutrients such as selenium and certain vitamins. To assess the thyroid disturbances in the mountain population of Benin, a survey was conducted in Natitingou, a mountain town located in the department of Atacora, in the northwest of Benin, on a sample of thirty (30) adults (15 men and 15 women), most of whom are educated. The results of the questionnaire revealed that 43% of the surveyed population acknowledged having knowledge on the mentioned subject and have dietary habits based on the consumption of seafood, and also legumes (20%). The examination of the serum results of the dosage of T3, T4 and TSH hormones revealed cases of thyroid disturbances in the region (36.32% in men and 44.98% in women). The analysis of a comparative table including the “VEDALAB Easy Reader+” and five (05) other readers, presents the performance, reading techniques, principles, advantages and disadvantages of each device. Pending further studies, some recommendations were made at the end of this study to the academic authorities regarding probable cases of dysthyroidism for which additional examinations are required and an awareness for the improvement of dietary habits.
基金This work was graciously supported by the Chinese National Natural Science Foundation(Grant 31901725 and 32201933)the Science and Technology Projects in Guangzhou(Grant 202201010170).
文摘Vaccarin,a flavonoid glycoside isolated from Vaccaria segetalis,is non-toxic to 3T3-L1 cells up to concentrations of 200μM.Accordingly,we investigated the effects of this natural product on adipogenesis and lipolysis in 3T3-L1 adipocytes.Our results revealed that vaccarin significantly inhibited lipid accumulation by suppressing the adipogenesis-related transcription factors peroxisome proliferator-activated receptorγ(PPARγ)and the CCAAT/enhancer-binding proteinα(C/EBPα).Specifically,lipid accumulation decreased by up to 27.7±2.7%when 3T3-L1 adipocytes were treated with a 10μM concentration of vaccarin.Mechanistic studies showed that the compound inhibited adipogenesis through activation of the Hedgehog(Hh)signaling pathway and so restoring Smo and Gli1 expression at an early stage of differentiation.In mature 3T3-L1 cells,vaccarin significantly increased the secretion of glycerol into the surrounding medium and thus indicating that it accelerated the degradation of triglycerides.In addition,vaccarin,was shown to enhance lipolysis through stimulation of the transcription levels of lipoprotein lipase,monoglycerides lipase,adipose triacylglyceride lipase,hormone-sensitive lipase and adipose differentiated-related protein.All told,vaccarin suppressed lipid accumulation and enhanced lipolysis during adipocyte differentiation by restoring Hh signaling.As such,it is a phytochemical capable of halting adipocyte hyperplasia and,thereby,ameliorating the effects of obesity.
